Parahippocampal corpora amylacea and neuronal lipofuscin in human aging

Open Medicine ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. 749-761
Author(s):  
Mirjana Bakić ◽  
Ivan Jovanović ◽  
Slađana Ugrenović ◽  
Ljiljana Vasović ◽  
Miljan Krstić ◽  
...  
Keyword(s):  
Periodic Acid ◽  
Age Groups ◽  
Neuron Specific Enolase ◽  
Parahippocampal Gyrus ◽  
Aging Brain ◽  
Numerical Density ◽  
Corpora Amylacea ◽  
Periodic Acid Schiff ◽  
Morphometric Analyses ◽  
Altered Metabolism

AbstractThe aim of this research was to quantify the number of corpora amylacea and lipofuscin-bearing neurons in the parahippocampal region of the brain. Right parahippocampal gyrus specimens of 30 cadavers were used as material for histological and morphometric analyses. A combined Alcian Blue and Periodic Acid-Schiff technique was used for identification and quantification of corpora amylacea and lipofuscin-bearing neurons. Immunohistochemistry was performed using S100 polyclonal, neuron-specific enolase and glial fibrillary acidic protein monoclonal antibodies for differentiation of corpora amylacea and other spherical inclusions of the aging brain. Cluster analysis of obtained data showed the presence of three age groups (median age: I = 41.5, II = 68, III = 71.5). The second group was characterized by a significantly higher numerical density of subcortical corpora amylacea and number of lipofuscin-bearing neurons than other two groups. Values of the latter cited parameters in the third group were insignificantly higher than the first younger group. Linear regression showed that number of parahippocampal lipofuscin-bearing neurons significantly predicts numerical density of subcortical corpora amylacea. The above results suggest that more numerous parahippocampal region corpora amylacea and lipofuscin-bearing neurons in some older cases might represent signs of its’ neurons quantitatively-altered metabolism.

scholarly journals Lipopolysaccharide, Identified Using an Antibody and by PAS Staining, Is Associated With Corpora amylacea and White Matter Injury in Alzheimer's Disease and Aging Brain

2021 ◽  
Vol 13 ◽  
Author(s):  
Xinhua Zhan ◽  
Marisa Hakoupian ◽  
Lee-Way Jin ◽  
Frank R. Sharp
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
White Matter ◽  
Periodic Acid ◽  
White Matter Injury ◽  
Aging Brain ◽  
Periventricular White Matter ◽  
Corpora Amylacea ◽  
Periodic Acid Schiff ◽  
Pas Staining

Corpora amylacea (CA) increase in number and size with aging. Their origins and functions remain unknown. Previously, we found that Alzheimer's disease (AD) brains have more CA in the periventricular white matter (PVWM) compared to aging controls. In addition, CA is associated with neurodegeneration as indicated by colocalization of degraded myelin basic protein (dMBP) with periodic acid-Schiff (PAS), a CA marker. We also found that bacterial lipopolysaccharide is present in aging brains, with more LPS in AD compared with controls. Periodic acid-Schiff staining is used to identify CA by virtue of their high polysaccharide content. Despite the growing knowledge of CA as a contributor to AD pathology, the molecules that contribute to the polysaccharides in CA are not known. Notably, lipopolysaccharides (LPS) are important cell-surface polysaccharides found in all Gram-negative bacteria. However, it is unknown whether PAS could detect LPS, whether the LPS found in aging brains contribute to the polysaccharide found in CA, and whether LPS associate with myelin injury. In this study, we found that aging brains had a myelin deficit zone (MDZ) adjacent to the ventricles in PVWM. The MDZ contained vesicles, most of which were CA. LPS and dMBP levels were higher in AD than in control brains. LPS was colocalized with dMBP in the vesicles/CA, linking white matter injury with a bacterial pro-inflammatory molecule. The vesicles also contained oxidized fibers, C-reactive protein, NG2, and GALC, markers of oligodendrocyte precursor cells (OPCs) and oligodendrocyte cells (OLs), respectively. The vesicles/CA were surrounded by dense astrocyte processes in control and AD brains. LPS was co-localized with CA by double staining of PAS with LPS in aging brains. The relationship of LPS with PAS staining was confirmed by PAS staining of purified LPS on nitrocellulose membranes. These findings reveal that LPS is one of the polysaccharides found in CA which can be stained with PAS. In addition, vesicles/CA are associated with oxidized and damaged myelin. The LPS in these vesicles/CA may have contributed to this oxidative myelin damage and may have contributed to oxidative stress to OPCs and OLs which could impair the ability to repair damaged myelin in AD and control brains.

Immunohistochemical Demonstration of Keratin in Canine Neuroepithelioma

1987 ◽  
Vol 24 (6) ◽  
pp. 500-503 ◽  
Author(s):  
W. Baumgärtner ◽  
P. V. Peixoto
Keyword(s):  
Spinal Cord ◽  
Glial Fibrillary Acidic Protein ◽  
Fourth Ventricle ◽  
Phosphotungstic Acid ◽  
Squamous Metaplasia ◽  
Periodic Acid ◽  
Neuron Specific Enolase ◽  
Periodic Acid Schiff ◽  
The Third ◽  
Intradural Extramedullary

Morphological features and immunoreactivity for cytokeratin (CK), glial fibrillary acidic protein (GFAP) and neuron-specific enolase (NSE) of three canine neuroepitheliomas and three canine ependymomas were investigated. Neuroepitheliomas were in three German shepherds as intradural-extramedullary solitary masses, with spinal cord displacement between T10 and L2. Histologically, they contained tubules and acini, lined by epithelial cells with focal squamous metaplasia, rosette-like structures, and polygonal to spindle-shaped cells between tubules. Acini were empty or filled with a homogeneous, eosinophilic periodic acid-Schiff (PAS)-positive material. Mitotic indices varied from low to moderate. Ependymomas occurred in the third (two cases) and fourth ventricle in adult boxers. Histologically, they were composed of cells with an ill-defined, scant amphophilic cytoplasm, with a central round euchromatic nucleus; cells formed pseudorosettes, with a central fibro-vascular stroma. Neuroepitheliomas stained for CK, but ependymomas did not. Both failed to stain for GFAP, NSE, or phosphotungstic acid hematoxylin (PTAH). Thus, antibodies to cytokeratin are useful to distinguish neuroepitheliomas from ependymomas.

Histologic and Immunohistochemical Studies of Granular Cell Tumors in Seven Dogs, Three Cats, One Horse, and One Bird

1993 ◽  
Vol 30 (2) ◽  
pp. 176-185 ◽  
Author(s):  
A. K. Patnaik
Keyword(s):  
Incidental Finding ◽  
Periodic Acid ◽  
Neuron Specific Enolase ◽  
Granular Cell ◽  
The Other ◽  
Intracranial Meningioma ◽  
Surgical Biopsy ◽  
Periodic Acid Schiff ◽  
Rhabdoid Cells ◽  
Eosinophilic Cytoplasm

In a retrospective study, granular cell tumors in six dogs (Nos. 1–6), three cats (Nos. 1–3), one horse (No. 1), and one cockatiel ( Nymphicus hollandicus) (No. 1) and a meningioma with a granular cell component in one dog (No. 7) were examined histologically and immunohistochemically. These tumors were identified by histologic examination of surgical biopsy specimens, except in the horse, in which the tumor was an incidental finding at necropsy. These diagnoses were initially made by more than one pathologist. Five of the six granular cell tumors in the dogs were in the oral cavity; one of these was in the maxillary gingiva of a 6-month-old puppy. The tumors in the cats were located in the tongue, vulva, and digit. The tumor in the horse was in the lung, and the tumor in the cockatiel was in the periocular tissue. Histologically, all granular cell tumors were characterized by oval to polygonal cells of various sizes. The cells had abundant, pale, eosinophilic cytoplasm with distinct intracytoplasmic granules, distinct cell margins, and mostly central nuclei. In the dogs, the gingival tumor had a large amount of collagen tissue, the tumor in the tongue had dilated blood vessels, and the maxillary tumor in the puppy was more cellular than the other tumors. The tumors in the cats were more anaplastic than the other tumors; one, located in the digit, was considered malignant. The granules in all of the tumors stained with periodic acid-Schiff and were diastase resistant. On staining with Luxol fast blue, the granules of all tumors stained different shades of pink, with the exception of the tumor in the tongue of a cat, which stained bluish green. Immunocytochemically, all tumors except the tumor in the cockatiel reacted against antibodies to vimentin. The granular cell tumor in the lung of the horse and the intracranial meningioma in a dog reacted to the antibody S-100 protein; the tumor in the horse reacted to neuron-specific enolase; tumors in two dogs (gingiva and skin) reacted to L-antitrypsin, and the maxillary tumor also reacted to lysozyme; the malignant tumor in the digit of a cat and the periocular tumor in the cockatiel reacted to muscle common actin and actin; the tumor in the cockatiel also reacted to desmin. Results of these immunocytochemical studies suggest that granular cell tumors, like tumors composed of rhabdoid cells, clear cells, and oncocytes, can have similar morphologic features but be of different cellular origins.

Canine Peripheral Nerve Sheath Tumor with Eosinophilic Cytoplasmic Globules

1998 ◽  
Vol 35 (3) ◽  
pp. 223-226 ◽  
Author(s):  
M. Kuwamura ◽  
J. Yamate ◽  
T. Kotani ◽  
T. Takeuchi ◽  
S. Sakuma
Keyword(s):  
Peripheral Nerve ◽  
Tumor Cells ◽  
Periodic Acid ◽  
Neuron Specific Enolase ◽  
Granular Cell ◽  
Nerve Sheath Tumor ◽  
Periodic Acid Schiff ◽  
Peripheral Nerve Sheath Tumor ◽  
Nerve Sheath ◽  
Spindle Cell Proliferation

A 13-year-old male Shetland Sheepdog had a subcutaneous tumor in the left brachium. The tumor was removed and recurred several times at 5, 13, 16, 22, and 31 months after the initial presentation. Histologically, the removed nodules from the fourth resection were composed of neoplastic proliferation of round to fusiform cells, which possessed eosinophilic globules in their cytoplasm. The globules were periodic acid–Schiff positive and diastase resistant. Positive reactions for acid phosphatase were observed in the cytoplasm of the tumor cells. Ultrastructurally, these globules consisted of membrane-bound, dense structures containing dense granules, lucent vacuoles, and homogeneous materials. The recurrent tumors removed at the fifth resection consisted of spindle cell proliferation arranged in interlacing fascicles with wavy nuclei and containing a small number of cells with cytoplasmic globules. The tumor cells were immunoreactive to vimentin, S-100 protein, myelin basic protein, and neuron-specific enolase. The tumor was diagnosed as a peripheral nerve sheath tumor with eosinophilic cytoplasmic globules. These findings are unique for the histogenesis of granular cell tumors.

scholarly journals Functional anatomy of the lacrimal gland in African black ostrich Struthio camelus domesticus in the embryonic and postnatal period

2015 ◽  
Vol 82 (1) ◽  
Author(s):  
Joanna Klećkowska-Nawrot ◽  
Karolina Goździewska-Harłajczuk ◽  
Renata Nowaczyk ◽  
Krzysztof Krasucki
Keyword(s):  
Morphological Changes ◽  
Periodic Acid ◽  
Age Groups ◽  
Functional Anatomy ◽  
Postnatal Period ◽  
Age Group ◽  
Periodic Acid Schiff ◽  
Struthio Camelus ◽  
Aldehyde Fuchsin ◽  
African Black

The aim of the present study was morphological and histochemical analysis of the lacrimalgland (LG) in African black ostrich Struthio camelus domesticus in the embryonic and postnatalperiod. Studies were conducted on 50 ostriches aged between the 28th day of incubation until7 months old. Tissue sections were stained with haematoxylin and eosin, Azan trichrome,periodic acid-Schiff, Alcian blue pH 2.5, aldehyde fuchsin and Hale’s dialysed iron. The LGin ostrich was classified as a tubulo-acinar type. The primordia of the lobes were determinedin the LG structure on the 28th day of incubation, whilst the weakly visible lobes with aciniand tubules were observed on the 40th day of incubation. Morphometric studies of the LGshowed steady growth, characterised by an increase in both length and width. Histometricmeasurements of lobe size showed little difference between the first, second and third agegroups, whilst in the fourth age group a marked increase in size of lobes was observed.The study showed that, apart from morphological changes, during the growth of the LGthe character of acid mucopolysaccharides changed. Sulphated acid mucopolysaccharideswere indicated, particularly with aldehyde fuchsin (AF) staining in the fourth age group.The Hale’s dialysed iron (HDI) staining showed a low concentration of carboxylated acidmucopolysaccharides in the first and second age groups and a higher concentration in thethird and fourth age groups. Periodic acid-Schiff staining (PAS)-positive cells were observedin each age group, but only a small number of cells with a weakly PAS-positive reaction weredemonstrated in the first age group.

scholarly journals Hypertrophic gastropathy associated with gastric sarcoma in a dog

2020 ◽  
Vol 33 (1) ◽  
pp. 112-115
Author(s):  
Mariarita Romanucci ◽  
Paolo E. Crisi ◽  
Maria Veronica Giordano ◽  
Morena Di Tommaso ◽  
Francesco Simeoni ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Periodic Acid ◽  
Neuron Specific Enolase ◽  
Partial Gastrectomy ◽  
Abdominal Ultrasound ◽  
Gastric Body ◽  
Gastric Glands ◽  
Periodic Acid Schiff ◽  
Gastric Lumen

A 14-y-old spayed female Labrador Retriever was presented with an 8-mo history of chronic vomiting. Abdominal ultrasound and gastrointestinal endoscopy revealed a mass protruding into the gastric lumen, with cytologic features suggestive of sarcoma. A partial gastrectomy was performed; the gastric body and antrum were thickened, with a cerebriform appearance of the mucosal surface. Histologic examination revealed a submucosal neoplastic proliferation of fusiform cells variably arranged in irregular bundles and scattered whorls. Fusiform cells strongly reacted to antibodies against vimentin, S100, and neuron-specific enolase; glial fibrillary acidic protein was moderately and multifocally expressed. Pancytokeratin, KIT, α–smooth muscle actin, and desmin were nonreactive. Histologic and immunohistochemical findings suggested a diagnosis of gastric sarcoma with features referable to a non-GIST (gastrointestinal stromal tumor), non–smooth muscle NIMT (non-angiogenic, non-lymphogenic intestinal mesenchymal tumor). The overlying gastric mucosa was thickened by elongated and dilated gastric glands, predominantly lined by intensely periodic acid-Schiff–stained mucous cells. This altered mucosal architecture was suggestive of Ménétrier-like disease. Although this disease has been hypothesized to predispose to gastric adenocarcinoma in dogs, an association with gastric sarcoma has not been documented previously in the veterinary literature, to our knowledge.

MO342ADMINISTRATION OF ASTRAGALUS MEMBRANACEUS PREVENTED CISPLATIN-INDUCED AKI, ESPECIALLY IN OLD MICE

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Kagemasa Kajiwara ◽  
Makoto Arai ◽  
Tatsuya Nogami ◽  
Yoshinobu Nakada ◽  
Go Nagashima ◽  
...  
Keyword(s):  
Technical Assistance ◽  
Periodic Acid ◽  
Critical Role ◽  
Age Groups ◽  
Astragalus Membranaceus ◽  
Control Group ◽  
Blood Collection ◽  
Periodic Acid Schiff ◽  
Old Mice ◽  
Young Mice

Abstract Background and Aims Recent findings suggest that acute kidney injury (AKI), which occurs frequently but is believed to be completely reversible, is an important factor driving chronic kidney disease (CKD) pathogenesis or progression. We have investigated Astragalus membranaceus (AM), which has been shown to have various pharmacological effects on several organs (see http://nccih.nih.gov/health/astragalus). However, up to now, little evidence of its effectiveness against CKD has been provided. We hypothesized that AM could target AKI and that sustainable prevention of AKI by AM might delay pathogenesis and progression of CKD. Here we focused on AKI as a promoter of CKD progression in order to examine the effects of AM histologically and histochemically in mice. Method Here we used two groups of female C57BL/6 mice: one that was aged 12 weeks and one that was aged 52 weeks. After the first blood collection (of approximately 0.2 ml), the two age groups of mice were administered AM powder mixed with sterilized 0.5% methylcellulose 400 (w/v) (the AM-administered group) or sterilized 0.5% methylcellulose 400 (the control group), respectively. Two hours after the administration, 0.5 mg/ml cisplatin (20 mg/kg or 14 mg/kg) or 0.9% NaCl were injected intraperitoneally. Three days after injection, blood was collected and kidneys were harvested. We measured blood urea nitrogen (BUN) and creatinine (CRE) to detect AKI, and assessed histological change in dissected kidney sections stained with Hematoxylin-Eosin and Periodic acid-Schiff and histochemical change in sections stained with anti-CD3 and anti-CD68 antibodies. Generally, 20 mg/kg cisplatin was used to induce AKI in the experimental model. Results Injection with 20 mg/kg cisplatin was shown to induce AKI pathology in young mice and shorten survival in old mice, and AM administration was unable to improve AKI pathology in young mice or survival in old mice. Next, we injected mice in both age groups with 14 mg/kg cisplatin. We found that this dose significantly increased serum BUN or CRE and caused histological damage in renal tubule epithelial cells and glomeruli in old mice but not in young mice, which showed no pathological change. And Astragalus treatment in advance almost totally prevented these pathological changes in old mice. The AKI generated in old mice with 14 mg/kg of cisplatin was significantly normalized by pretreatment with AM. Next, histochemical analysis of renal CD3- and CD68-positive cells revealed both were increased in the murine AKI model induced by injection with 20 mg/kg cisplatin. Interestingly, in old mice, 14 mg/kg cisplatin-AKI increased CD3-positive cells but not CD68-positive cells. These findings suggest that AM may improve daily minor disturbances, such as AKI, that cause pathogenesis and progression of CKD especially in old age. Conclusion AM administration, at least in part, can reduce day-to-day AKI occurrence, but is ineffective in young kidneys. However, sustained use of AM could play a critical role in prolonging the activity of aged kidneys. Acknowledgments We thank the Education and Research Support Center, Tokai University, for technical assistance. This work was supported by a grant from the Japan Society for Promotion of Science JSPS KAKENHI KIBAN-C (Grant Number 16K09259) and by Tokai university general research organization grant.

scholarly journals Gastroesophageal tube of the Iguana iguana (Iguanidae): histological description, histochemical and immunohistochemical analysis of 5-HT and SS cells

2023 ◽  
Vol 83 ◽  
Author(s):  
A. O. Serra-Campos ◽  
A. N. G. Abreu-Junior ◽  
A. A. Nascimento ◽  
M. Abidu-Figueiredo ◽  
M. S. C. S. Lima ◽  
...  
Keyword(s):  
Adaptive Response ◽  
Unit Area ◽  
Periodic Acid ◽  
Immunohistochemical Analysis ◽  
Goblet Cells ◽  
Numerical Density ◽  
Periodic Acid Schiff ◽  
Iguana Iguana ◽  
Cervical Oesophagus ◽  
Pseudostratified Epithelium

Abstract The work aims were to describe the histological and histochemical structure of the gastroesophageal tube of Iguana iguana and verify the occurrence and distribution of immunoreactive serotonin (5-HT) and somatostatin (SS) cells. Fragments of the gastrointestinal tract (GIT) of five iguanas were which underwent standard histological and immunohistochemistry technique. Immunoreactive cells for 5-HT and SS were quantified using the STEPanizer. The oesophagus has ciliated columnar pseudostratified epithelium with staining Alcian blue (AB) + and goblet cells highly reactive to periodic acid Schiff (PAS). In the cervical oesophagus, the numerical density of 5-HT cells per unit area (QA [5-HT cells]/µm2) was 4.6x10-2 ± 2.0 and celomatic oesophagus presented QA = 4.0x10-2 ± 1.0. The epithelium of the stomach is simple columnar, PAS and AB +. The cranial and middle regions of the stomach presented (QA [5-HT cells]/µm2) = 6.18x10-2 ± 3.2 and the caudal region, QA = 0.6x10-2 ± 0.2. The SS cells were only observed in the caudal stomach, with numerical density (QA [SS cells]/µm2) = 1.4x10-2 ± 0.9 In I. iguana, variation was observed in terms of the distribution of mucus secretions and the pattern of occurrence of serotonin and somatostatin-secreting enteroendocrine cells in the TGI, which possibly will result in an interspecific adaptive response.

Brain Lesions in Aging Zoo-Housed Naked Mole-Rats (Heterocephalus glaber)

2020 ◽  
pp. 030098582096998
Author(s):  
Jerrold M. Ward ◽  
Andrew N. Cartoceti ◽  
Martha A. Delaney
Keyword(s):  
Hippocampal Neurons ◽  
Brain Aging ◽  
Periodic Acid ◽  
Limited Information ◽  
Corpora Amylacea ◽  
Aging Research ◽  
Periodic Acid Schiff ◽  
Heterocephalus Glaber ◽  
Cytoplasmic Vacuoles ◽  
Cerebellar Purkinje Cells

Naked mole-rats (NMRs) are common in the managed care of zoos and valuable models for aging research. Limited information on NMR neuropathology is available despite many studies regarding their aging physiology. Histologic sections of brain from 27 adult (5–27 years old) NMRs from 2 zoos were reviewed to determine presence or absence of lesions associated with advanced age in humans and other mammals. A majority (23/27; 85%) of NMR brains had cerebral cortical neuronal changes with rounded or angular neurons, cytoplasmic vacuoles containing pale yellow pigment, periodic acid–Schiff (PAS)-positive granules and green autofluorescence, compatible with lipofuscinosis. Less severe lesions were present in cerebellar Purkinje cells, medulla, and hippocampal neurons. The hypothalamic neuropil of all NMRs had scattered variably sized PAS-positive granules and 10 (37%) had larger round bodies consistent with corpora amylacea. The youngest NMRs, 5 to 7 years old, generally had minimal or no cerebrocortical lesions. Further studies will help understand brain aging in this long-lived species.

scholarly journals The histomorphological pattern of various tonsillar lesions. A 3-year study in the tertiary care center.

2020 ◽  
Vol 6 (8) ◽  
pp. 486-492
Author(s):  
Dr. Ritu Sharma ◽  
◽  
Dr. Megha Bansal ◽  
Dr. (Brig.) Nikhilesh Kumar ◽  
Dr. Gaurav Kumar ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Care Center ◽  
Periodic Acid ◽  
Tertiary Care ◽  
Age Groups ◽  
Lymphoid Hyperplasia ◽  
Chronic Tonsillitis ◽  
Reactive Lymphoid Hyperplasia ◽  
Periodic Acid Schiff ◽  
Pathogenic Agents

Introduction: Palatine tonsils are paired masses of lymphoid tissue, which act as an immunologicbarrier against the entry of pathogenic agents into the respiratory and digestive tracts. Tonsillitis is acommon disease, especially among children. Chronic tonsillitis is a disease with repeated attacks ofacute tonsillitis or a sub-clinic form of a resistant or poorly treated infection. Aim: To determine theclinicopathological findings in various lesions of tonsillectomy in the palatine tonsil. Materials andmethods: This is a retrospective study where 105 (Unilateral-20, Bilateral) cases ofhistopathologically identified tonsillectomy specimens as well as 4 tonsillar biopsies were included.The available data for all the patients as regards age, sex, and clinical symptoms were collected.Sections were taken from tonsillectomy specimens from representative areas while tonsillar biopsieswere processed completely. Routine hematoxylin and eosin staining was done along with this specialstain- PAS (periodic acid- Schiff) for actinomycosis and ZN stain for AFB. Results: Amongst thecases, 78 cases showed chronic tonsillitis. 11 cases were chronic tonsillitis with actinomycosis, acutechronic tonsillitis in 07 cases, granulomatous tonsillitis in 03 cases, acute ulcerative tonsillitis withmicroabscesses in 01 case, and reactive lymphoid hyperplasia in 02 cases. Three malignancies wereobserved – Two cases of squamous cell carcinoma and one case of undifferentiatedcarcinoma/lymphoma. Conclusion: Chronic tonsillitis is a common problem facing in all age groups,Histopathology plays a significant role in diagnosing both benign and malignant lesions of the tonsil.

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