Reproductive characteristics of a precocious vaccine line (Rt3+15) of Eimeria tenella in embryonating chicken eggs

2006 ◽  
Vol 51 (2) ◽  
Author(s):  
D. Grant Richards ◽  
Kevin Whithear ◽  
Peter Scott ◽  
Robin Gasser
Keyword(s):  
Economic Importance ◽  
Eimeria Tenella ◽  
Intestinal Disease ◽  
Chicken Embryos ◽  
Reproductive Characteristics ◽  
Precocious Line ◽  
Chicken Eggs

AbstractCoccidiosis of chickens, caused by species of Eimeria (Protozoa, Apicomplexa), is an intestinal disease of major economic importance worldwide. In the present study, the reproductive characteristics of a precocious line (designated E. tenella Rt3+15) from Australia were investigated in chicken embryos and the implications of the findings briefly discussed.

scholarly journals Optimum conditions for cultivation of Eimeria tenella in chicken embryos.

1982 ◽  
Vol 44 (6) ◽  
pp. 951-956
Author(s):  
Yutaka NAKAI ◽  
Keiji OGIMOTO ◽  
Masanori KATSUNO
Keyword(s):  
Eimeria Tenella ◽  
Optimum Conditions ◽  
Chicken Embryos

scholarly journals Simplified Method for Efficient Intravascular Inoculation of Chicken Embryos

1976 ◽  
Vol 4 (1) ◽  
pp. 104-105
Author(s):  
C. L. Kelling ◽  
I. A. Schipper
Keyword(s):  
Vascular Trauma ◽  
Chicken Embryos ◽  
Simplified Method ◽  
Chicken Eggs ◽  
Embryonated Chicken ◽  
Embryonic Death

The simple syringe-stabilizer unit described in this note provides a means for rapid intravascular inoculation of embryonated chicken eggs with minimal embryonic death from vascular trauma.

Altering the surface area available for transport of calcium from the eggshell does not affect calcium metabolism of chicken embryos

1991 ◽  
Vol 69 (12) ◽  
pp. 3084-3087
Author(s):  
Mary J. Packard
Keyword(s):  
Surface Area ◽  
Calcium Metabolism ◽  
Phosphorus Content ◽  
Chorioallantoic Membrane ◽  
Chicken Embryos ◽  
Calcium Magnesium ◽  
Inner Aspect ◽  
Chicken Eggs

The amount of contact between the chorionic surface of the chorioallantoic membrane and the inner aspect of the eggshell was reduced by causing formation of an extra air cell in chicken eggs on day 8 of incubation. This manipulation reduced the surface area available for transport of calcium from the eggshell to the embryo. Concentrations of calcium, magnesium, and phosphorus in serum were determined for embryos sampled on days 12, 15, and 18, and for hatchlings. Calcium, magnesium, and phosphorus content of residual yolks and yolk-free carcasses of hatchlings was also determined. The presence of an extra air cell did not lead to variation in any of these indices of calcium metabolism. One interpretation of these results is that embryos compensate for the reduction in surface area available for transport of calcium by increasing the transport of calcium across those areas of the chorioallantois that remain in contact with the eggshell.

scholarly journals The Neonicotinoid Thiacloprid Interferes with the Development, Brain Antioxidants, and Neurochemistry of Chicken Embryos and Alters the Hatchling Behavior: Modulatory Potential of Phytochemicals

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Mayada R. Farag ◽  
Mahmoud Alagawany ◽  
Attia A. A. Moselhy ◽  
Enas N. Said ◽  
Tamer A. Ismail ◽  
...  
Keyword(s):  
Brain Activity ◽  
In Ovo ◽  
Neuroprotective Effects ◽  
Chicken Embryos ◽  
Complementary Effect ◽  
Brain Antioxidants ◽  
Chicken Eggs ◽  
The Brain ◽  
Different Doses

The present experiment was performed to investigate the toxic impact of thiacloprid (TH) on the brain of developing chicken embryos and also to measure its influence on the behavioral responses of hatchlings. The role of chicoric acid (CA) and rosmarinic acid (RA) in modulating the resulted effects was also investigated. The chicken eggs were in ovo inoculated with TH at different doses (0.1, 1, 10, and 100 ug/egg). TH increased the mortality and abnormality rates and altered the neurochemical parameters of exposed embryos dose-dependently. TH also decreased the brain level of monoamines and amino acid neurotransmitters and decreased the activities of acetylcholine esterase (AchE) and Na+/K+-ATPase. The brain activity of catalase (CAT) and superoxide dismutase (SOD) was diminished with downregulation of their mRNA expressions in the brain tissue. When TH was co-administered with CA and RA, the toxic impacts of the insecticide were markedly attenuated, and they showed a complementary effect when used in combination. Taken together, these findings suggested that TH is neurotoxic to chicken embryos and is possibly neurotoxic to embryos of other vertebrates. The findings also demonstrated the antioxidant and neuroprotective effects of CA and RA. Based on the present findings, the CA and RA can be used as invaluable ameliorative of TH-induced toxicity.

scholarly journals TOXICITY TEST OF INDIVIDUAL AND COMBINED TOXIC EFFECTS OF GLYPHOSATE HERBICIDE AND HEAVY METALS ON CHICKEN EMBRYOS

AGROFOR ◽  
2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Rita SZABÓ ◽  
Dalma CSONKA ◽  
László MAJOR ◽  
József LEHEL ◽  
Péter BUDAI
Keyword(s):  
Heavy Metals ◽  
Body Weight ◽  
The Body ◽  
Embryo Mortality ◽  
Developmental Anomalies ◽  
Chicken Embryos ◽  
Post Test ◽  
Test Materials ◽  
The Individual ◽  
Chicken Eggs

The aim of this study was to determine the individual and combined toxicity of Glialka Star herbicide (glyphosate 360 g/l) and heavy metals (copper and cadmium) on the development of chicken embryos. On the first day of incubation, chicken eggs were injected by 0.1 ml of the test materials. The applied concentration of copper and cadmium sulphate was 0.01% and that of herbicide Glialka Star was 2%. The chicken embryos were examined on day 19 by the followings: rate of embryo mortality, body mass, type of developmental anomalies by macroscopic examination. The body weight was evaluated statistically by oneway ANOVA combined with Dunnett post-test, the embryo mortality and the developmental anomalies were analysed by Fisher test. Our teratogenicity study revealed that the combined administration of heavy metals (copper, cadmium) and glyphosate (K-salt) containing herbicide formulation caused significant reduction in the body weight of embryos and a significant increase in the rate of embryonic mortality and the incidence of developmental anomalies. The joint toxic effect of heavy metals and Glialka Star is an additive effect compared to the individual toxicity of the test materials.

scholarly journals In situ DNA transfer to chicken embryos by biolistics

1999 ◽  
Vol 22 (4) ◽  
pp. 525-529 ◽  
Author(s):  
Luciana A. Ribeiro ◽  
João L. Azevedo ◽  
Francisco J.L. Aragão ◽  
Elibio L. Rech ◽  
Gilberto S. Schmidt ◽  
...  
Keyword(s):  
Survival Rate ◽  
Dna Transfer ◽  
Lacz Gene ◽  
Galactosidase Activity ◽  
Cmv Promoter ◽  
Gold Particles ◽  
Chicken Embryos ◽  
Helium Gas ◽  
Chicken Eggs

Fertilized chicken eggs were bombarded with a biolistic device. Transient expression of the lacZ gene under the control of a human cytomegalovirus (CMV) promoter was assessed after in situ gene transfer using this approach. The influence of different pressures, vacuum levels and particles was tested. Survival rate improved as particle velocity decreased, but resulted in lower levels of expression. The best survival and expression were obtained with gold particles, a helium gas pressure of 600 psi and a vacuum of 600 mmHg. Under these conditions, all bombarded embryos showed <FONT FACE="Symbol">b</FONT>-galactosidase activity, indicating that this was an effective method for transformation of chicken embryos.

scholarly journals Distinct Intensity of Host-Pathogen Interactions in Chlamydia psittaci- and Chlamydia abortus-Infected Chicken Embryos

2012 ◽  
Vol 80 (9) ◽  
pp. 2976-2988 ◽  
Author(s):  
Maria Braukmann ◽  
Konrad Sachse ◽  
Ilse D. Jacobsen ◽  
Martin Westermann ◽  
Christian Menge ◽  
...  
Keyword(s):  
Immune Response ◽  
Chorioallantoic Membrane ◽  
Chlamydia Psittaci ◽  
Internal Organs ◽  
Content Type ◽  
Chlamydia Abortus ◽  
Chicken Embryos ◽  
Related Proteins ◽  
Chicken Eggs ◽  
Infectious Potential

ABSTRACTFactors and mechanisms determining the differences in virulence and host specificity between the zoonotic agentsChlamydia psittaciandChlamydia abortusare still largely unknown. In the present study, two strains were compared for their invasiveness, virulence, and capability of eliciting an immune response in chicken embryos. On breeding day 10, embryonated chicken eggs were inoculated with 5 × 104inclusion-forming units. As shown by immunohistochemistry and quantitative real-time PCR,C. psittacidisplayed a significantly better capability of disseminating in the chorioallantoic membrane (CAM) and internal organs thanC. abortus. The higher infectious potential ofC. psittaciin birds was underlined by significantly higher mRNA expression rates of essential chlamydial genes, such asincA,groEL(in CAM, liver, and spleen),cpaf, andftsW(in CAM). Although the immune responses to both pathogens were similar,C. psittacielicited higher macrophage numbers and a stronger expression of a subset of immune-related proteins. The data imply that invasiveness ofChlamydiaspp. and propagation in the host are not solely dependent on the level of host immune response but, even to a greater extent, on the expression of bacterial factors related to virulence. The fact thatC. psittacihas coped far better thanC. abortuswith the avian embryo's response by upregulating essential genes may be a key to understanding the mechanisms underlying host adaptation and etiopathology.

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