The article is presented daily dynamics of the magnesium using of cow’s mammary gland during the new lactation period. During the time from milking to milking the breast tissue in the new lactation period absorbed and released Magnesium into the flowing out blood. In general, the use of Magnesium by the tissues of the cow’s mammary gland during the new lactation period during the time of the first milking ranged from 1.44±0.288 to 1.46±0.292 mmol/l. Magnesium from the incoming blood. For the fourth hour after milking, the breast tissue of the cows was absorbed only 0.01±0.002 mmol/l of Magnesium, or 0.68%. By the sixth hour after milking the breast tissue secrete 0.01 ± 0.002 mmol/l of Magnesium in the flowing out blood, and they absorbed the same amount of Magnesium for eight hours after milking. It should be noted that during the period from the first to the second milking breast tissue absorbed only 0.003±0.0006 mmol/l of Magnesium, which is 0.21 % of its content in arterial blood. From the second to the third milking breast tissue used Magnesium in small quantities. In the second hour after milking, they absorbed 0.02 ± 0.004 mmol/l of Magnesium from the incoming blood. In the fourth and sixth hours of the second milking only 0.70 % of Magnesium content in the arterial blood (0.01±0.002 mmol/l) was used by breast tissue. At eight hours after milking, breast tissue secretes 0.70 % of Magnesium (0.01±0.002 mmol/l) into the blood. From the second to the third milking, on average, the cow’s breast tissue of absorbed 0.007±0.0014 mmol/l of Magnesium, which is 1.48 % of its content in the arterial blood. During the period from the third evening to the first morning milking breast tissues for the second and fourth hours after the third milking absorbed 0.01±0.002 mmol/l of Magnesium. In the sixth and eighth hours after milking the cow’s breast tissue excreted 0.01±0.002 mmol/l of Magnesium into flowing out from the breast blood. During the period from the third to the first milking cow’s breast tissue absorbed only 0.005±0.001 mmol/l of Magnesium, which is 1.4 times less (p<0.001) than after the second milking, which is 0.35 %. On average per day cow’s breast tissue absorbed 0.005±0.001 mmol/l of Magnesium, which is only 0.35 % of its content in arterial blood. During the day, the breast tissue of cows in the period of milking isolated Magnesium in the blood from the breast at the level of 0.28 %.During the period of lactation recession the use of Magnesium by cows breast tissue had a corresponding characteristic. It should be noted, breast tissue of cows was excreted Magnesium in the flowing out blood only for the second hour after milking during the period from the first to the second milking (- 0.01±0.002 mmol/l, or 0.69 %). Breast tissue of cows absorbed Magnesium at the level 0.01±0.002 mmol/l in the next hour after the milking. Breast tissue of the cows did not absorb Magnesium from the incoming to them blood in the sixth and eighth hours after milking the. Breast tissue of cows did not adsorb Magnesium from the blood flowing to the breast the incoming blood in the period from the third to the first milking. Breast tissue absorbed it from incoming blood and released into the flowing out blood wavily during the period from the second to the third milking. In the second hour after milking the breast tissue of cows excreted 0.01±0.002 mmol/l of Magnesium into the blood, for the fourth hour absorbed Magnesium in the same amount. Magnesium only on the second hour after milking. In the following, at the fourth, sixth and eight hours after milking breast tissue was excreted in the flowing out from the breast of cows blood at the level of 0.02±0.004 mmol/l and 0.01±0.002 mmol/l of Magnesium. On average, from the third (evening) to the first (morning) milking of the breast tissue excreted Magnesium into the flowing out blood at the level -0.002±0.0004 mmol/l, or 0.13 %. In general during the day, breast tissue of cows during the period of lactation recession, Magnesium was excreted into the flowing out blood at the level of 0.02±0.004 mmol/l, or 0.14 %, which is 2.5 times and 1.5 times less than in the middle of lactation less than in the new-period of lactation (p<0.01).