Isolation and Identification Candida spp from Urine and Antifungal Susceptibility Test

2018 ◽  
Vol 59 (4B) ◽  
Keyword(s):  
Antifungal Susceptibility ◽  
Susceptibility Test ◽  
Candida Spp ◽  
Isolation And Identification ◽  
Antifungal Susceptibility Test

scholarly journals Antifungal susceptibility of clinically significant candida species by disk diffusion method

2021 ◽  
Vol 7 (2) ◽  
pp. 77-80
Author(s):  
Ajitha Reddy Edula
Keyword(s):  
Antifungal Agents ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Susceptibility Test ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Candida Spp ◽  
Disk Diffusion Method ◽  
Antifungal Susceptibility Test

Antifungal susceptibility of candida. To perform antifungal susceptibility testing on candida isolates by disk diffusion method & study its susceptibility pattern. The present study was conducted in the department of Microbiology in a tertiary care hospital in Hyderabad from January 2013 to June 2014, with prior approval of the Institutional Ethics Committee. The present study was designed to perform antifungal susceptibility test on Candida isolates by Disk Diffusion Method and study its susceptibility pattern. 102 Candida isolates were subjected to Antifungal susceptibility testing by Disk diffusion method using Mueller-Hinton Agar + 2% Glucose and 0.5 μg/mL Methylene Blue Dye (GMB) Mediumas per CLSI guidelines. : Antifungal susceptibility test shows that C. albicans is more susceptible to all the antifungal agents tested. Resistance to azole group of drugs was more pronounced in non-albicans candida spp. Voriconazole seemed to be superior to Fluconazole with a better susceptibility in the Fluconazole resistant strains also.Findings of the antifungal susceptibility test suggest that Candida spp., differ in their susceptibility to antifungal agents. Antifungal susceptibility testing of Candida isolates will be helpful in guiding physicians to select the appropriate antifungal drug so that therapeutic failures can be avoided thus decreasing patient morbidity and mortality.

scholarly journals Evaluation of semisolid agar method for antifungal susceptibility test of T. rubrum

2016 ◽  
Vol 7 (1) ◽  
pp. 11
Author(s):  
Sultana Razia ◽  
Shahida Anwar ◽  
Md. Ruhul Amin Miah ◽  
Najmun Nahar ◽  
Ripon Barua
Keyword(s):  
Antifungal Agents ◽  
Antifungal Susceptibility ◽  
Antifungal Drugs ◽  
Test Method ◽  
Susceptibility Test ◽  
Broth Microdilution ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Antifungal Susceptibility Test ◽  
Mic Value

<p><strong>Background:</strong> With increasing fungal disease many newer antifungal drugs are available with different spectrum of activ­ity. Antifungal susceptibility test will help clinicians for selection of effective drug and thereby treatment of patient.<strong> </strong></p><p><strong>Objective:</strong> The study was undertaken to perform a simple screening drug susceptibility test of T. rnbrum by Semi Solid Agar Antifungal Susceptibility (SAAS) <strong></strong></p><p><strong>Method:</strong> Perfonnance of susceptibility method was assessed by comparing the MICs of three commonly prescribed antifungal agents namely- tluconazole (FCZ), itraconazole (ITZ) and terbinafine (TER) to the CLSI (Clinical and Laboratory Standard Institute) recommended M-38, a broth microdilution method. <strong></strong></p><p><strong>Results:</strong> In SAAS method, among twenty nine T. rubrum, twenty five (86.2%) were susceptible (MIC range 0.5-64 µg/ml) to Fluconazole (FCZ) and four (13.7%) were resistant (MIC value &gt;64 µg/ml). In broth microdilution method, among twenty nine T. rubrum, twenty six (89.6%) were susceptible (MIC range 0.3-64 µg/ml) to FCZ and three (10.3%) were resistant (MIC value &gt;64 µg/ml). In case of both ITZ and TER, all were susceptible (MIC range 0.3-64 µg/ml) to both methods. The SAAS method demonstrated the susceptibility pattern of T. rubrum against FCZ, ITZ and TER usually within 72 to 96 hours after organism isolation and results were concordance with the results of CLSI broth microdilution method. <strong></strong></p><p><strong>Conclusion:</strong> Though it is a newer method with proper standardization of the test method, SAAS method is simple and easily applicable screening method for susceptibility testing of antifungal agents against dermatophytes in any microbiology laboratories.</p>

Comparative analysis of three antifungal susceptibility test methods against prospectively collected Candida species

1994 ◽  
Vol 18 (2) ◽  
pp. 89-94
Author(s):  
Kara M. Villareal ◽  
Rebecca A. Cook ◽  
John N. Galgiani ◽  
Richard P. Wenzel ◽  
Peter G. Pappas ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Candida Species ◽  
Antifungal Susceptibility ◽  
Test Methods ◽  
Susceptibility Test ◽  
Antifungal Susceptibility Test

scholarly journals Evaluation of a Colorimetric Antifungal Susceptibility Test by Using 2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride

2004 ◽  
Vol 48 (11) ◽  
pp. 4457-4459 ◽  
Author(s):  
Jeong Hwan Shin ◽  
Jae-Cheol Choi ◽  
Jeong Nyeo Lee ◽  
Hyung Hoi Kim ◽  
Eun Yup Lee ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Colorimetric Assay ◽  
Antifungal Susceptibility ◽  
Susceptibility Test ◽  
National Committee ◽  
Tetrazolium Chloride ◽  
Antifungal Susceptibility Test

ABSTRACT A colorimetric antifungal susceptibility test was performed using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride. Among 24 strains of Candida species, no trailing growth was found. In 22 and 20 strains, the MICs obtained in the colorimetric assay were within two dilutions of those obtained by the National Committee for Clinical Laboratory Standards method for ketoconazole and itraconazole, respectively.

Sign in / Sign up

Export Citation Format

Share Document