scholarly journals Karakterisasi Senyawa Bioaktif Antimikroba Ekstrak Umbi Bawang Tiwai (Eleutherine Bulbosa (MILL.) URB.)

2019 ◽  
Vol 2 (1) ◽  
pp. 63-69
Author(s):  
Mahfuzun Bone ◽  
Yusnita Rifai ◽  
Gemini Alam
Keyword(s):  
1H Nmr ◽  
E Coli

bulbosa adalah tanaman khas suku Kalimantan yang telah digunakan secara turun-temurun sebagai antibakteri. Penelitian ini bertujuan untuk menentukan karakterisasi senyawa aktif antimikroba E. bulbosa. Tahapan penelitian diawali dengan ekstraksi E.bulbosa dengan pelarut metanol dan partisi cair-cair menggunakan pelarut n-heksan, etil asetat, n-butanol. Uji Skrining antimikroba ekstrak methanol, n-heksan, etil asetat, dan n-butanol dengan konsentrasi 1 mg/mL. Ekstrak n-heksan difraksinasi dengan kromatografi cair vakum dan direfraksinasi kembali menggunakan sepacore. Fraksi aktif diisolasi dengan kromatografi lapis tipis preparatif dan diperoleh isolat aktif E. bulbosa. Hasil Identifikasi berdasarkan kromatogram menunjukkan isolat aktif E.bulbosa adalah golongan naphthalene. interpretasi data spektroskopi FTIR isolat aktif E.bulbosa menunjukkan adanya gugus OH hidroksil, CH alifatik, dan C=O. Hasil Interpretasi data 1H NMR isolat aktif E. bulbosa menunjukkan Data 1H NMR memperlihatkan adanya spektrum dari atom H-6 dengan nilai δ 6,92 ppm Mult. doblet dan J 10,5 Hz, spektrum dari atom H-8 dengan nilai δ 7,75 ppm, Mult. doblet dan J 10,5 Hz, spektrum dari atom H-9 dengan nilai δ 6,29 ppm, dan spektrum H-11 dengan nilai δ 3,21 ppm. Nilai Konsentrasi Hambat Minimum (KHM) isolat aktif E.bulbosa terhadap bakteri uji V. cholera, B. subtilis, S. mutans, S. aureus 0,0125% dan bakteri E. coli 0,025%. Disimpulkan bahwa isolat aktif E. bulbsoa adalah golongan senyawa naftalen dan memiliki aktivitas antimikroba.  

scholarly journals 1H NMR spectroscopy-based metabolomics analysis for the diagnosis of symptomatic E. coli-associated urinary tract infection (UTI)

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Milena Lussu ◽  
Tania Camboni ◽  
Cristina Piras ◽  
Corrado Serra ◽  
Francesco Del Carratore ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Nmr Spectroscopy ◽  
Tract Infection ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
E Coli ◽  
Metabolomics Analysis

scholarly journals Synthesis, Characterization and Molecular Docking Studies of Mn (II) Complex of Sulfathiazole

2019 ◽  
pp. 95-102
Author(s):  
I. E. Otuokere ◽  
J. G. Ohwimu ◽  
K. C. Amadi ◽  
C. O. Alisa ◽  
F. C. Nwadire ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Charge Transfer ◽  
1H Nmr ◽  
Ir Spectrum ◽  
Antibacterial Drug ◽  
Organosulfur Compounds ◽  
Monodentate Ligand ◽  
Ligand Complex ◽  
Sulfa Drug ◽  
E Coli

Sulfathiazole (SFTZ) is an antibacterial drug that contains the organosulfur compounds. It is used as a short-acting sulfa drug. The metal complexes of sulfa-drug have gained considerable importance due to their pronounced biological activity. The sulfa-drugs have received great attention because of their therapeutic applications against bacterial infections. Mn(II) complex of sulfathiazole was synthesized by the reaction of sulfathiazole with MnCl2.4H2O. The Mn (II) complex was characterized based on UV, IR, 1H NMR Spectroscopy and x-ray powder diffraction. The electronic spectrum of the ligand showed intra charge transfer which was assigned to the chromophores present in the ligand, while that of the complex suggested intra ligand charge transfer (ILCT) and ligand to metal charge transfer (LMCT). In the IR spectrum of sulfathiazole, the N-H stretch of SO2NH appeared at 3255.23 cm-1. In the IR spectrum of the metal complex, this band was absent. This suggested the deprotonation of the N-H of SO2NH during the complexation reaction. This showed that sulfathiazole acted as a monodentate ligand. 1H NMR spectrum of [Mn(SFTZ)] complex showed the involvement of the nitrogen atom of SO2NH. The crystal structure of [Mn(SFTZ)] complex belongs to monoclinic system, space group P1, with cell parameters of a= 4.519Å, b = 8.704Å, c = 12.608Å, V = 493.5Å3, β = 95.69º. Molecular docking suggested that the ligand/complex bonded effectively with the E.coli and S.aureus because their global binding energies were negative. The binding interactions of ligand/complex with E. coli and S. aureus were predicted. Molecular docking predicted the feasibility of the biochemical reactions before experimental investigation. It was concluded that sulfathiazole behaved as a monodentate ligand towards Mn (II) ion. The binding energy and interaction of [Mn(SFTZ)] with E.coli and S. aureus have also shown that inhibition of the bacterial species is feasible. The mechanism of action of [Mn(SFTZ)] with E. coli and S. aureus is now well understood.

Synthesis of new photobactericidal polymers by “click chemistry” and a study of their biological activity

e-Polymers ◽  
2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Abdelmajid Memmi ◽  
Robert Granet ◽  
Mahjoub Aouni ◽  
Amina Bakhrouf ◽  
Pierre Krausz
Keyword(s):  
Biological Activity ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Addition Reaction ◽  
Fatty Ester ◽  
E Coli ◽  
Plastic Materials ◽  
Covalently Linked ◽  
High Yields

AbstractPhotobactericidal plastic materials have been synthesized from cellulose and natural or synthetic porphyrins. The photosensitizer was covalently linked to a cellulose fatty ester by means of a Huisgen cyclo addition reaction resulting in high yields and degrees of substitution (DS). The polymers obtained were characterized by UV vis., IR and 1H NMR spectroscopy. Photobactericidal properties were evaluated on the three pathogenic strains E. coli, S. aureus and P. aeruginosa. A total killing of the bacteria was achieved with plastics with DS values higher than 1% upon illumination.

scholarly journals STRUCTURE ELUCIDATION OF ANTIBACTERIAL COMPOUND FROM Ficus deltoidea Jack LEAVES

2011 ◽  
Vol 11 (1) ◽  
pp. 67-70 ◽  
Author(s):  
Suryati Suryati ◽  
Hazli Nurdin ◽  
Dachriyanus Dachriyanus ◽  
Md Nordin Hj Lajis
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Spectroscopic Data ◽  
Structure Elucidation ◽  
Antibacterial Activities ◽  
Ficus Deltoidea ◽  
E Coli ◽  
Minimum Inhibition Concentration ◽  
Antibacterial Compound ◽  
Inhibition Concentration

An antibacterial compound has been isolated from Ficus deltoidea Jack leaves. Based on spectroscopic data (IR, 1H-NMR, 13C NMR 1D and 2D and MS), the structure of this compound was identified as 3β-hydroksilup-20(29)-en, (lupeol), C30H50O. This compound showed antibacterial activities against E. coli, B. subtilis and S. aureus. The minimum inhibition concentration (MIC) against E. coli, B. subtilis and S. aureus are 150, 220 and 130 μg/mL respectively.

scholarly journals Synthesis and Evaluation of Antimicrobial Activities of New Functional Derivatives of 3-[5-(4-Nitrophenyl)-2-Furyl]-4-Pyrazole-Carbaldehydes

2021 ◽  
Vol 11 (4) ◽  
pp. 12159-12169
Keyword(s):  
1H Nmr ◽  
Antimicrobial Agents ◽  
Ethyl Cyanoacetate ◽  
Synthesis Method ◽  
Antimicrobial Activities ◽  
E Coli ◽  
Functional Derivatives ◽  
Composition And Structure ◽  
Biological Potential ◽  
Derivatives Of

The analysis of the biological potential of derivatives of 4-alkenyl- and imino functionalized pyrazoles is carried out, based on which the expediency of design of new structures with pharmacophore 5-(4-nitrophenyl)furanyl fragment is substantiated. Their synthesis method using a structural modification of 3-[5-(4-nitrophenyl)furan-2-yl]pyrazole-4-carbaldehyde to the corresponding alkenyl derivatives under the action of malononitrile, ethyl cyanoacetate, cyanoacetamide, and thioxoimidazolidine is proposed. The hydrazones, (thio)semicarbazones, and oximes were obtained by the condensation of corresponding aldehydes with hydrazides, (thio)semicarbazides, and hydroxylamine. The synthesized compounds' composition and structure were determined by elemental analysis, IR, and 1H NMR spectra. The fact existence of a mixture of E/Z-isomers among the series of obtained hydrazones of 1-phenyl-4-pyrazolecarbaldehydes was determined, and the quantitative ratio of geometric isomers was determined using 1H NMR spectroscopy data. The results of the microbiological evaluation of the synthesized pyrazole derivatives showed that they have a pronounced effect on strains of bacteria S. aureus ATCC 25923, E. coli ATCC 25922, and fungi of the genus Candida and are promising for the creation of effective antimicrobial agents.

scholarly journals Synthesis and Antimicrobial Studies of Novel N-Glycosyl Hydrazino Carbothioamide

2020 ◽  
Vol 33 (1) ◽  
pp. 127-131
Author(s):  
Riddhi A. Nayak ◽  
Anvita D. Mangte
Keyword(s):  
Antimicrobial Activity ◽  
1H Nmr ◽  
Medicinal Chemistry ◽  
Antimicrobial Activities ◽  
Spectral Studies ◽  
Mass Spectral ◽  
E Coli ◽  
Antimicrobial Studies ◽  
Antimicrobial Evaluation

In view of applications of N-glycosylated compounds in medicinal chemistry and in many other ways, herein the synthesis of novel N-glycosyl hydrazino carbothioamides is reported. New N-glycosyl hydrazino carbothioamides were synthesized by the condensation of per-O-acetyl glycosyl isothiocyanate with different aromatic hydrazides. The newly synthesized compounds were characterized by using the IR, 1H NMR and mass spectral studies. Antimicrobial evaluation of the synthesized N-glycosyl hydrazino carbothioamide was also examined. Antimicrobial activities of the synthesized compound were evaluated against bacteria E. coli, P. aeruginosa, S. aureus, S. pyogenus and fungi C. albicans, A. niger and A. clavatus. All the N-glycosyl hydrazino carbothioamides exhibit promising antimicrobial activity.

scholarly journals The SYNTHESIS, SCREENING OF NOVEL 1-SUBSTITUTED-3-(4-OXO-2-PHENYLQUINAZOLIN-3(4H)-YL) UREA AND THIOUREA ANALOGUES AS POTENT ANTIBACTERIALS

2019 ◽  
pp. 38-42
Author(s):  
SANDHYA R. DHOKALE ◽  
SNEHAL R. THAKAR ◽  
DEEPALI A. BANSODE ◽  
KAKASAHEB R. MAHADIK
Keyword(s):  
Antibacterial Activity ◽  
Melting Point ◽  
1H Nmr ◽  
Ir Spectrum ◽  
Mass Spectra ◽  
Substitution Pattern ◽  
Thiourea Derivatives ◽  
E Coli ◽  
Capillary Method

Objective: The proposed study is an attempt to determine antibacterial activity of synthesized novel 1-substituted-3-(4-oxo-2-phenylquinazolin-3(4H)-yl) urea and thiourea analogues as potent antibacterials against S. aureus and E. coli bacteria. Methods: The present study reports new series of 1-substituted-3-(4-oxo-2-phenylquinazolin-3(4H)-yl) urea and thiourea derivatives as potent antibacterial agents. Reagents used in the present study were of synthetic grade and solvents were used after distillation. Novel quinazolinone analogues were synthesized by considering substitution pattern, characterization of the synthesized analogues was performed using various techniques like Thin layer chromatography, Melting point, Infrared spectroscopy, Proton NMR spectrometry and Mass spectrometry. TLC of the synthesized analogues was carried out by using (toluene: methanol in the ratio 2:1), melting point was found by open capillary method, IR spectrum was recorded on JASCO V-530, 1H NMR was recorded on Bruker Avance Spectrometer and Mass spectra were obtained from G6460A, triple quadrupole/MS/MS system. In vitro antibacterial activity was performed against S. aureus and E. coli. Results: Six derivatives of quinazolinone analogues were synthesized. The structures of 1-substituted-3-(4-oxo-2-phenylquinazolin-3(4H)-yl) urea and thiourea derivatives were confirmed by physical and spectral analysis. Synthesized molecules showed Rf of 0.45-0.80 in toluene: methanol mobile phase, melting point was carried out by open capillary method and were in range of 90-210 ° C, IR spectrum was recorded in range of 14000-400 cm-1and showed characteristic peaks of NH and of C-O-NH, 1H NMR of the compounds was distinct to confirm structures with delta values in the range of 7.53-11.960, Mass spectra proved parent peaks of synthesized compounds confirming molecular weight. The compounds were assayed for antibacterial activity against S. aureus and E. coli using ciprofloxacin as standard. The synthesized analogues have shown good yield and comparable antibacterial. Conclusion: The present study delivers a convenient and efficient protocol for the quinazolinone analogues synthesis.

Synthesis of Novel Oxazin Analogs of Thio-4-azaspiro[4.5]decan-3-one for their Antimicrobial Activity

2021 ◽  
Vol 6 (2) ◽  
pp. 116-120
Author(s):  
Raj Bahadur Singh ◽  
Krishna Srivastava ◽  
Ram Prakash Tiwari ◽  
Jyoti Srivastava
Keyword(s):  
Antimicrobial Activity ◽  
Hydrazine Hydrate ◽  
1H Nmr ◽  
Absorption Spectroscopy ◽  
Elemental Analysis ◽  
Spectral Studies ◽  
Good Activity ◽  
Mass Spectral ◽  
E Coli

The present work reports the synthesis of 4-(1-(substituted phenyl)-1H-naphtho[1,2-e][1,3]oxazin- 2(3H)-yl)-1-thia-4-azaspiro-[4.5]-decan-3-one IV(a-h) by 4-(((substituted phenyl)(2-hydroxynaphthalen- 1-yl) ethyl)amino)-1-thia-4-azaspiro[4.5]decan-3-one with formaldehyde in acetonitrile, containing a spiro ring obtained from the reaction of cyclohexylidene hydrazine and thioglycollic acid in DMF (cyclohexanone reacts with hydrazine hydrate in pyridine). The structures of the synthesized compounds have been established on the basis of elemental analysis, UV-vis absorption spectroscopy, IR, 1H NMR and mass spectral studies. The in vitro antimicrobial screening of all novel compounds was done against S. aureus, E. coli, P. aeruginosa and B. subtilis. The activity of compounds IVb, IVc, IVe and IVf compounds showed moderate to good activity against the tested microbes.

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