Application of the fluorescent antibody method for rapid diagnosis of viral lesions of the anterior segment of the eye

2020 ◽  
pp. 189-190
Author(s):  
N.R. Marchenko ◽  
◽  
E.A. Kasparova ◽  
A.A. Kasparov ◽  
M.A. Makarova ◽  
...  
Keyword(s):  
Fluorescent Antibody ◽  
Anterior Segment ◽  
Rapid Diagnosis ◽  
Antibody Method ◽  
Fluorescent Antibody Method

Localization of pancreatic enzymes in ultrathin frozen sections stained with metal labeled antibody

1978 ◽  
Vol 36 (2) ◽  
pp. 90-91
Author(s):  
Kenjiro Yasuda
Keyword(s):  
Fluorescent Antibody ◽  
Pancreatic Enzymes ◽  
Tissue Preparation ◽  
Zymogen Granules ◽  
Frozen Sections ◽  
En Bloc ◽  
Antibody Method ◽  
Ultrathin Frozen Sections ◽  
Fluorescent Antibody Method

Localization of amylase,chymotrypsinogen and trypsinogen in pancreas was demonstrated by Yasuda and Coons (1966), by using fluorescent antibody method. These enzymes were naturally found in the zymogen granules. Among them, amylase showed a diffuse localization around the nucleus, in addition to the zymogen granules. Using ferritin antibody method, scattered ferritin granules were also found around the Golgi area (Yasuda et al.,1967). The recent advance in the tissue preparation enables the antigen to be localized in the ultrathin frozen sections, by applying the labeled antibodies onto the sections instead of staining the tissue en bloc.The present study deals with the comparison of the localization of amylase and lipase demonstrated by applying the bismuth-labeled, peroxidase-labeled and ferritin-labeled antibody methods on the ultrathin frozen sections of pancreas, and on the blocks of the same tissue.

Identification of Group A Beta-Hemolytic Streptococci by the Fluorescent Antibody Method

1978 ◽  
Vol 143 (11) ◽  
pp. 782-784
Author(s):  
Jerome B. Myers ◽  
John W. Crum ◽  
Lynn P. Beaulieu
Keyword(s):  
Fluorescent Antibody ◽  
Antibody Method ◽  
Group A ◽  
Fluorescent Antibody Method

Comparison of detection specificity of nitrifying bacteria in biofilm using fluorescence in situ hybridization and in situ fluorescent antibody methods

2003 ◽  
Vol 47 (5) ◽  
pp. 129-132
Author(s):  
N. Noda ◽  
Y. Ebie ◽  
M. Matsumura ◽  
S. Tsuneda ◽  
A. Hirata ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Specific Fluorescence ◽  
Antibody Method ◽  
In Situ Detection ◽  
Detection Specificity ◽  
Fluorescent Antibody Method

The in situ fluorescent antibody and fluorescence in situ hybridization (FISH) methods are very useful in the in situ detection of specific bacteria like nitrifiers in a biofilm. In this study, simultaneous staining using the FISH and in situ fluorescent antibody methods was examined. As a result, no specific fluorescence was observed with either method when FISH was performed followed by the in situ fluorescent antibody method; however, when the in situ fluorescent antibody method was performed first followed by FISH, specific fluorescence was observed in both cases. Moreover, it was suggested that the detection specificities of FISH and the in situ fluorescent antibody method are almost identical.

scholarly journals Fluorescent Antibody Method of Detecting Gonorrhea in Asymptomatic Females

1961 ◽  
Vol 76 (2) ◽  
pp. 93 ◽  
Author(s):  
Ad Harris ◽  
W. E. Deacon ◽  
John Tiedemann ◽  
William L. Peacock, Jr.
Keyword(s):  
Fluorescent Antibody ◽  
Antibody Method ◽  
Fluorescent Antibody Method
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