Novel multiplex immunoassay for simultaneous detection of treponemal and nontreponemal antibodies in patients with syphilis infection

2016 ◽  
Author(s):  
Chisanga Lwatula
Keyword(s):  
Simultaneous Detection ◽  
Syphilis Infection ◽  
Multiplex Immunoassay

F.53. Development of a Quantitative Multiplex Immunoassay Panel for the Simultaneous Detection of 32 Cytokines/Chemokines in Mouse Sample

2008 ◽  
Vol 127 ◽  
pp. S60
Author(s):  
Yao Chen ◽  
Jiaxin Dong ◽  
Deborah Droll ◽  
Michelle Grigsby ◽  
Jehangir Mistry ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Multiplex Immunoassay ◽  
Mouse Sample

Rapid, Sensitive, and Simultaneous Detection of Three Foodborne Pathogens Using Magnetic Nanobead–Based Immunoseparation and Quantum Dot–Based Multiplex Immunoassay

2011 ◽  
Vol 74 (12) ◽  
pp. 2039-2047 ◽  
Author(s):  
HONG WANG ◽  
YANBIN LI ◽  
ANDREW WANG ◽  
MICHAEL SLAVIK
Keyword(s):  
Salmonella Typhimurium ◽  
Fluorescence Intensity ◽  
Foodborne Pathogens ◽  
Pathogenic Bacteria ◽  
Human Life ◽  
Simultaneous Detection ◽  
Food Product ◽  
Food Samples ◽  
Multiplex Immunoassay ◽  
E Coli

Losses caused by foodborne diseases are enormous in terms of human life, illness, medical costs, and food product recalls. Rapid detection of multiple bacterial pathogens in foods is extremely important to ensure food safety. The objective of this research was to develop a multiplex immunoassay by integrating magnetic nanobeads (MNBs) for immunoseparation with quantum dots (QDs) as fluorescent labels for rapid, sensitive, and simultaneous detection of three major pathogenic bacteria, Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes, in food products. In this research, both streptavidin-conjugated MNBs (30- and 150-nm diameter) and QDs (530-, 580-, and 620-nm emission wavelength) were separately coated with biotinylated anti-Salmonella, anti–E. coli, and anti-Listeria antibodies. The immuno-MNBs were mixed with a food sample to capture the three target bacteria. After being magnetically separated from the sample, the MNB-cell conjugates were mixed with the immuno-QDs to form the MNB-cell-QD complexes, and unattached QDs were removed. The fluorescence intensity of the MNB-cell-QD complexes was measured at wavelengths of 530, 580, and 620 nm to determine the populations of Salmonella Typhimurium, E. coli O157:H7, and L. monocytogenes, respectively. This multiplex immunoassay simultaneously detected Salmonella Typhimurium, E. coli O157:H7, and L. monocytogenes at levels as low as 20 to 50 CFU/ml in food samples in less than 2 h without enrichment. The change in fluorescence intensity was linearly correlated (R2 > 0.96) with the logarithmic value of bacterial level in the range of 10 to 103 CFU/ml. More than 85% of the three target pathogens could be simultaneously separated from food samples. The multiplex immunoassay could be expanded to detect more target pathogens, depending on the availability of specific antibodies and QDs with different emission wavelengths.

scholarly journals Simultaneous Detection of Haemophilus influenzae Type b Polysaccharide-Specific Antibodies and Neisseria meningitidis Serogroup A, C, Y, and W-135 Polysaccharide-Specific Antibodies in a Fluorescent-Bead-Based Multiplex Immunoassay

2009 ◽  
Vol 16 (3) ◽  
pp. 433-436 ◽  
Author(s):  
Richarda M. de Voer ◽  
Rutger M. Schepp ◽  
Florens G. A. Versteegh ◽  
Fiona R. M. van der Klis ◽  
Guy A. M. Berbers
Keyword(s):  
Haemophilus Influenzae ◽  
Neisseria Meningitidis ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Haemophilus Influenzae Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Specific Antibodies ◽  
Multiplex Immunoassay

ABSTRACT We expanded the meningococcal serogroup A, C, Y, and W-135 multiplex immunoassay (MIA) to simultaneously detect immunoglobulin type G antibodies directed toward Haemophilus influenzae type b polysaccharide (HibPS). The monoplex HibPS assay was compared to a HibPS-specific competitive enzyme-linked immunosorbent assay and showed a good correlation (R = 0.96). Furthermore, no cross-reactivity between HibPS and the four meningococcal serogroups was detected. This pentaplex meningococcal Hib MIA is a useful tool to investigate serological responses toward different childhood PS vaccines.

Syphilis Infection Rates in U.S. Jumped 12% From 2006 to 2007

2008 ◽  
Vol 38 (8) ◽  
pp. 5
Author(s):  
MICHELE G. SULLIVAN
Keyword(s):  
Infection Rates ◽  
Syphilis Infection

Species Specific Immunoassays to Measure Blood Platelet and Coagulation Activation in the Rat

1996 ◽  
Vol 76 (06) ◽  
pp. 1090-1095 ◽  
Author(s):  
C Ravanat ◽  
M Freund ◽  
S Schuhler ◽  
P Grunert ◽  
L Meyer ◽  
...  
Keyword(s):  
Antithrombin Iii ◽  
Plasma Concentrations ◽  
Simultaneous Detection ◽  
Blood Platelet ◽  
Coagulation Activation ◽  
Platelet Factor ◽  
Prethrombotic State ◽  
Low Levels ◽  
Species Specific ◽  
Higher Sensitivity

SummaryThe purpose of this study was to develop specific and sensitive immunoassays to detect early indices of hypercoagulability in the rat. Rat platelet factor 4 (rPF4) and rat fibrinopeptide A (rFPA) assays, tools for the detection of activation of platelets and coagulation respectively, were designed using antibodies raised against purified rPF4 and against synthetic rFPA. The relevance of these new assays and of the commercially available ELISA kit for thrombin-antithrombin III (TAT) complexes was demonstrated in a rat model of a prethrombotic state induced by intravenous infusion of varying doses of thromboplastin (90 to 2400 μl/kg/h). In this model, the immunoassays allowed simultaneous detection of low levels of rFPA and rPF4 which were correlated with fibrinogen and platelet consumption and TAT generation and further proved to be of higher sensitivity than the classical methods of platelet count or measurement of fibrinogen levels. Plasma concentrations of rFPA, rPF4 and TAT were dependent on infusion time and thromboplastin dose, while hirudin (1 mg/kg) prevented their appearance. Thus the new specific immunoassays for rPF4 and rFPA and the commercial human TAT assay represent useful tools for pathophysiological studies or the screening of antithrombotic drugs in rats.

The antiphospholipid syndrome in patient after syphilis infection

2019 ◽  
pp. 63-62
Author(s):  
M.V. Zhuchkov ◽  
◽  
E.E. Bolshakova ◽  
D.B. Sonin ◽  
◽  
...  
Keyword(s):  
Antiphospholipid Syndrome ◽  
Syphilis Infection
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