Single Nucleotide Polymorphism of ADIPOQ Gene (Rs822393) Is Associated with Lipid Profile, Adiponectin Levels and Ratio of Adiponectin/Leptin ratio in Adult Obese Subjects

This study was conducted to assess the possible association between polymorphisms of the transcription factor 7-like 2 (TCF7L2) gene and obese Iraqi adults. DNA was obtained from 158 obese subjects and 142 controls. Two specific PCR fragments were designed to incorporate two highly frequent single nucleotide polymorphism (SNP)s within TCF7L2, rs11196208 and rs7908486. Both amplified loci were genotyped by PCR-single-strand conformation polymorphism (SSCP) followed by sequencing. Logistic regression analysis was performed to detect the association between both genetic variants and obesity. Concerning rs11196208-based amplicons, PCR-SSCP genotyping showed homogeneous banding patterns for all investigated samples. In contrast with rs11196208, three SSCP banding patterns in the rs7908486-based amplicons, GG, AG and AA, were observed. Individuals with the AA genotype showed significantly higher (P<0.05) body mass index (BMI), waist circumference (WC), fasting blood glucose (FBG), and insulin values than those with either AG or GG genotypes. Association analysis revealed that individuals with the A allele exhibited a greater risk of obesity than individuals with the B allele. Data indicate that rs7908486 SNP exerted a tight association with obesity. The study suggests implementing TCF7L2 rs7908486-based genotyping in the early detection of obesity.

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Link between single nucleotide polymorphism of rs266729 and rs2241766 in the ADIPOQ gene and gestational diabetes in an Iranian population

Gene Reports ◽

10.1016/j.genrep.2018.11.009 ◽

2019 ◽

Vol 14 ◽

pp. 72-75 ◽

Cited By ~ 1

Author(s):

Fatemeh Nezamzadeh ◽

Aylin Esmailkhani ◽

Elahe Edalati ◽

Seyedeh Saeedeh Hosseini ◽

Ahmad Ghasemi ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

Gestational Diabetes ◽

Iranian Population ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Adipoq Gene

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THE ROLE OF GENETIC VARIATION SINGLE NUCLEOTIDE POLYMORPHISM T45G AND SINGLE NUCLEOTIDE POLYMORPHISM G276T OF THE ADIPONECTIN GENE IN FATTY LIVER PATHOMECHANISM ON OBESE MALE SUBJECT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11s1.26615 ◽

2018 ◽

Vol 11 (13) ◽

pp. 229

Author(s):

Sulaeman A ◽

Wahid S ◽

Sulaiman A ◽

Lawrence Sg

Keyword(s):

Insulin Resistance ◽

Single Nucleotide Polymorphism ◽

Genetic Variation ◽

Fatty Liver ◽

Adiponectin Gene ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Obese Subjects ◽

High Level ◽

Obese Male

Objective: The aim of this study was to observe the role of genetic variation T45G and G276T of adiponectin gene in pathomechanism of fatty liver on obese subjects.Methods: An observational study with case–control design was conducted on 94 obese male subjects (50 subjects are obese male with fatty liver and 44 subjects are obese male without fatty liver). The research is taken place in Prodia Clinical Laboratory Makassar to determine genetic variation single nucleotide polymorphism (SNP) T45G (Genotype TT, thyroglobulin [TG], and GG) and SNP G276T (Genotype GG, GT, and TT) of adiponectin gene, technique of polymerase chain reaction-restriction fragment length polymorphism was used. The level of adiponectin, soluble tumor necrosis factor-α receptor 2 (sTNF-αR2), and insulin serum were measured by enzyme-linked immunosorbent assay method, meanwhile, fatty liver was detected by ultrasonography.Results: The result of the study showed genetic variation, T45G of adiponectin gene was genotype TT 62.8 %, genotype TG 30.9%, and genotype GG 6.3%; meanwhile, genetic variation G276T of adiponectin gene was genotype GG 43.6%, genotype GT 38.3 %, and genotype TT 18.1 %. There was no significant correlation of genetic variation T45G as well as G276T of adiponectin gene, to the level of adiponectin serum (>0.05). Insulin resistance was more frequent on genotype TT genetic variation T45G of adiponectin gene as compare to that on TG+GG (p=0.069). Genotype TT on genetic variation T45G of adiponectin gene was significant correlated with fatty liver (p=0.010). Genotype TG+TT on genetic variation G276T of adiponectin gene was more likely to have insulin resistance and fatty liver than that of genotype GG. Allele T carrier on genetic variation T45G and G276T of adiponectin gene had a higher chance to have insulin resistance and fatty liver as compare to that of allele G carrier. The odds ratio of having fatty liver insulin resistance is 5.3, genotype TT on genetic variation T45G of adiponectin gene is 3.8, low level of adiponectin is 3.4, and high level of sTNF-αR2 is 3.3.Conclusion: Genotype TT on genetic variation T45G of adiponectin gene has a role in fatty liver on obese subjects. Genotype TG+TT on genetic variation G276T of adiponectin gene was more high frequent to have fatty liver compare to that on genotype GG. Allele T carrier on genetic variation T45G of adiponectin gene had higher frequency on the occurrence of insulin resistance than that on allele G carrier. Insulin resistance has the highest influence as compare to genotype TT on genetic variation T45G of adiponectin gene, low level of adiponectin serum, high level of sTNF-αR2 serum in the pathomechanism of fatty liver in obese subjects.

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