EFFECTS OF LIPOPOLYSACCHARIDEINDUCED ENDOTOXEMIA ON OOCYTE MEIOTIC MATURATION, DNA DAMAGE AND VIABILITY OF OVARIAN GRANULOSA CELLS IN MICE

2018 ◽  
Author(s):  
O. Kondratska ◽  
N. Grushka ◽  
N. Makogon ◽  
S. Pavlovych ◽  
R. Yanchii
Keyword(s):  
Dna Damage ◽  
Granulosa Cells ◽  
Meiotic Maturation ◽  
Ovarian Granulosa Cells ◽  
Oocyte Meiotic Maturation

Effects of DNA damage and short-term spindle disruption on oocyte meiotic maturation

2014 ◽  
Vol 142 (2) ◽  
pp. 185-194 ◽  
Author(s):  
T. Zhang ◽  
G. L. Zhang ◽  
J. Y. Ma ◽  
S. T. Qi ◽  
Z. B. Wang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Meiotic Maturation ◽  
Short Term ◽  
Oocyte Meiotic Maturation

Doxorubicin Exposure Affects Oocyte Meiotic Maturation through DNA Damage-Induced Meiotic Arrest

2019 ◽  
Vol 171 (2) ◽  
pp. 359-368 ◽  
Author(s):  
Zhi-Ming Ding ◽  
Shou-Xin Zhang ◽  
Xiao-Fei Jiao ◽  
Li-Ping Hua ◽  
Muhammad Jamil Ahmad ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cancer Patients ◽  
Polar Body ◽  
Young Female ◽  
Meiotic Maturation ◽  
Antitumor Antibiotic ◽  
Meiotic Arrest ◽  
First Polar Body ◽  
Female Cancer Patients ◽  
Oocyte Meiotic Maturation

Abstract Developments in chemotherapeutics have enhanced the survival rate of cancer patients, however, adverse effects of chemotherapeutics on ovarian functions causes the fertility loss in young female cancer patients. Doxorubicin (DOX), as an anthracycline antitumor antibiotic, is extensively used to cure various malignancies. Recent studies have suggested that DOX can cause ovarian damage and affect the oocyte maturation, nevertheless the mechanism by which DOX on oocytes meiosis is poorly understood. In this study, we explored the mechanism for DOX-induced oocytes meiotic failure in vitro at human relevant exposure levels and time periods. Results described that DOX (100 nM) can interrupt the mouse oocytes meiotic maturation directly with reduced first polar body extrusion. Cell cycle analysis showed that most oocytes were arrested at metaphase I (MI) stage. However, DOX treatment had no effect on spindle structure but chromosomal misalignment. We observed that kinetochore-microtubule structure was affected and the spindle assemble checkpoint was provoked after DOX treatment. Moreover, severe DNA damage was found in DOX-treated oocytes indicated by the positive γ-H2A.X foci signal, which then may trigger oocytes early apoptosis. Besides, metaphase II oocytes with disorganized spindle morphologies and misaligned chromosomes were observed after DOX treatment. In conclusion, DOX have the potential to disrupt oocyte meiotic maturation through DNA damage induced meiotic arrest mediated by spindle assemble checkpoint activation. These findings can contribute to design the new therapies to alleviate DNA damage to preserve fertility for young female cancer patients with chemotherapeutics.

Ultrastructural localisation of calcium deposits in pig oocytes maturing in vitro: effects of verapamil

Zygote ◽  
2003 ◽  
Vol 11 (3) ◽  
pp. 253-260 ◽  
Author(s):  
J. Rozinek ◽  
Z. Vaňourková ◽  
M. Sedmíkova ◽  
V. Lánská ◽  
J. Petr ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Channel Blocker ◽  
Meiotic Maturation ◽  
Oocyte Nucleus ◽  
Calcium Dependent ◽  
Calcium Deposits ◽  
In Vitro Effects ◽  
Oocyte Meiotic Maturation ◽  
Metaphase I

The culture of pig oocytes in the presence of the calcium channel blocker verapamil (0.02 mM) resulted in the blocking of meiosis at the metaphase I stage, and only a small fraction (about 28%) of the oocytes were able to continue their maturation to the stage of metaphase II. Hence, meiotic maturation in pig oocytes is a calcium-dependent process. After isolation of the pig oocytes from their follicles, the intracellular calcium deposits in the oocyte and granulosa cells, detectable using the combined oxalate-pyroantimonate method, are depleted. The amount of calcium deposits in the oocyte and granulosa cells increased during oocyte meiotic maturation in vitro, especially in the nucleus, mitochondria, vacuoles and cytoplasm. The replenishment of calcium deposits is significantly changed under the effect of verapamil. The increase in calcium deposits in the oocyte nucleus was delayed, a much larger amount of deposits was formed in the mitochondria, and the amount of deposits in the vacuoles was demonstrably smaller. A significant peak in the accumulation of calcium deposits was observed in the cytoplasm of verapamil-treated oocytes after 16 h of in vitro culture. We propose that an altered pattern in the replenishment of calcium deposits can disturb intracellular signalling and prevent the exit of oocytes from the metaphase I stage.

scholarly journals The Function of Cumulus Cells in Oocyte Growth and Maturation and in Subsequent Ovulation and Fertilization

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2292
Author(s):  
Bongkoch Turathum ◽  
Er-Meng Gao ◽  
Ri-Cheng Chian
Keyword(s):  
Granulosa Cells ◽  
Follicular Development ◽  
Cumulus Cells ◽  
Oocyte Quality ◽  
Early Embryo ◽  
Developmental Competence ◽  
Meiotic Maturation ◽  
Supporting Cells ◽  
Oocyte Growth ◽  
Oocyte Meiotic Maturation

Cumulus cells (CCs) originating from undifferentiated granulosa cells (GCs) differentiate in mural granulosa cells (MGCs) and CCs during antrum formation in the follicle by the distribution of location. CCs are supporting cells of the oocyte that protect the oocyte from the microenvironment, which helps oocyte growth and maturation in the follicles. Bi-directional communications between an oocyte and CCs are necessary for the oocyte for the acquisition of maturation and early embryonic developmental competence following fertilization. Follicle-stimulation hormone (FSH) and luteinizing hormone (LH) surges lead to the synthesis of an extracellular matrix in CCs, and CCs undergo expansion to assist meiotic resumption of the oocyte. The function of CCs is involved in the completion of oocyte meiotic maturation and ovulation, fertilization, and subsequent early embryo development. Therefore, understanding the function of CCs during follicular development may be helpful for predicting oocyte quality and subsequent embryonic development competence, as well as pregnancy outcomes in the field of reproductive medicine and assisted reproductive technology (ART) for infertility treatment.

scholarly journals Bisphenol B Exposure Disrupts Mouse Oocyte Meiotic Maturation in vitro Through Affecting Spindle Assembly and Chromosome Alignment

2020 ◽  
Vol 8 ◽  
Author(s):  
Shou-Xin Zhang ◽  
Zhi-Ming Ding ◽  
Muhammad Jamil Ahmad ◽  
Yong-Sheng Wang ◽  
Ze-Qun Duan ◽  
...  
Keyword(s):  
Dna Damage ◽  
Spindle Assembly ◽  
Epigenetic Modifications ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Meiotic Arrest ◽  
First Polar Body ◽  
Chromosome Alignment ◽  
Oocyte Meiotic Maturation

Bisphenol B (BPB), a substitute of bisphenol A (BPA), is widely used in the polycarbonate plastic and resins production. However, BPB proved to be not a safe alternative to BPA, and as an endocrine disruptor, it can harm the health of humans and animals. In the present study, we explored the effects of BPB on mouse oocyte meiotic maturation in vitro. We found that 150 μM of BPB significantly compromised the first polar body extrusion (PBE) and disrupted the cell cycle progression with meiotic arrest. The spindle assembly and chromosome alignment were disordered after BPB exposure, which was further demonstrated by the aberrant localization of p-MAPK. Also, BPB exposure increased the acetylation levels of α-tubulin. As a result, the spindle assemble checkpoint (SAC) was continuously provoked, contributing to meiotic arrest. We further demonstrated that BPB severely induced DNA damage, but the ROS and ATP production were not altered. Furthermore, the epigenetic modifications were changed after BPB exposure, as indicated by increased K3K9me3 and H3K27me3 levels. Besides, the pattern of estrogen receptor α (ERα) dynamics was disrupted with a mass gathering on the spindle in BPB-exposed oocytes. Our collective results indicated that exposure to BPB compromised meiotic maturation and damaged oocyte quality by affecting spindle assembly and chromosome alignment, acetylation of α-tubulin, DNA damage, epigenetic modifications, and ERα dynamics in mouse oocytes.

cDNA cloning reveals the potential of human ovarian granulosa cells to secrete collagenase inhibitor

1989 ◽  
Vol 120 (3_Suppl) ◽  
pp. S138
Author(s):  
J. FREUDENSTEIN ◽  
J. MUCHA ◽  
G. RAPP ◽  
K. H. SHEIT
Keyword(s):  
Cdna Cloning ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells ◽  
Collagenase Inhibitor
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