scholarly journals Piperine Levels in Java Chili and Black Fruits Extracts from Regions with Different Altitude

2021 ◽  
Vol 6 (1) ◽  
pp. 16-22
Author(s):  
Ni Putu Ermi Hikmawanti ◽  
Endang Hanani ◽  
Shintia Maharani ◽  
Ajeng Istiningtyas Wahyudi Putri
Keyword(s):  
High Altitude ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Pepper Fruit ◽  
Uv Visible ◽  
Low Altitude ◽  
Piper Retrofractum ◽  
Layer Chromatography

Piper retrofractum Vahl. (java chili or cabe jawa) and Piper nigrum L. (black pepper or lada hitam) are parts of the Piperaceae. One of the chemical constituents found in the fruit of the two plants is piperine, which has properties as antimicrobial, antidiabetic, anti-inflammatory, anticancer etc. This study aims to determine the piperine levels contained in 95% ethanol extract of java chili and black pepper fruits from regions with different altitude using UV-Visible spectrophotometer. Each sample were extracted by maceration method using 95% ethanol. Qualitative identification of piperine was carried out using thin layer chromatography (TLC). Determination of piperine levels was carried out with UV-Visible spectrophotometer at maximum wavelength of 253.8 nm. The results showed that the levels of piperine contained in the 95% ethanol extract of java chili fruit from low altitude (Lampung), medium altitude (Madura) and high altitude (Bogor) are 1.54±0.02%; 1.44±0.02%; and 1.41±0.02% (w/v), respectively. Whereas the levels of piperine contained in the 95% ethanol extract of black pepper fruit from low altitude (East Luwu), medium altitude (Central Lampung) and high altitude (Bogor) are 5.63±0.14%; 5.38±0.05%; and 4.87±0.09% (w/v), respectively.

scholarly journals PENETAPAN KADAR PIPERIN DALAM EKSTRAK BUAH LADA HITAM (Piper nigrum Linn.) MENGGUNAKAN LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC–MS/MS)

2018 ◽  
Vol 1 (2) ◽  
pp. 69-79
Author(s):  
Alifia Putri Febriyanti ◽  
Siti Jazimah Iswarin ◽  
Susanti Susanti
Keyword(s):  
Quantitative Analysis ◽  
Ethanol Extract ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Value Recovery ◽  
Pepper Fruit ◽  
Validation Parameters ◽  
Liquid Chromatography Tandem Mass ◽  
Layer Chromatography ◽  
Dark Blue

The utilization of medicinal plants have started growing rapidly in the world, including in Indonesia, along with the thinking back to nature, one of the plants that are often used as a medicine is black pepper fruit (Piper nigrum Linn.). Piperine is a major compound and potent substances contained in black pepper fruit as antidiarrheal activity. The aim of this study was to determine levels of piperine in 96% ethanol extract of black pepper fruit. The extraction method used Soxhlet in 96% ethanol as solvent. Analysis of piperine using qualitative analysis by thin layer chromatography (TLC) produces yellow stain after being sprayed by dragendorf and dark blue on observation Uv-Vis 365 nm with Rf extract value 0.49 and Rf piperine value 0.5. Quantitative analysis using LC – MS produces piperine levels at 26%. Before quantitative analysis, validation method needs to be done with the parameters, among others: linearity have regression equation y = has 1,981,691.1333x + 561,445.0000 and correlation coefficient (R2) of 0.9973, LOD and LOQ at 0.14 and 0.44 ppm, accuracy value (% recovery) between 95.90 – 100.77%, precision value (% KV) between 0.02 – 1.84%, and selectivity (RT) between 2.47 – 2.49. The results of the validation parameters are eligible so that piperine levels were obtained by LC – MS declared accurate, specific, and precise.

scholarly journals Cycloartane-Type Triterpenes and Botanical Origin of Propolis of Stingless Indonesian Bee Tetragonula sapiens

Plants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 57 ◽  
Author(s):  
Niken Pujirahayu ◽  
Toshisada Suzuki ◽  
Takeshi Katayama
Keyword(s):  
High Performance ◽  
Chemical Constituents ◽  
Mangifera Indica ◽  
Ethanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Botanical Origin ◽  
Characteristic Peak ◽  
Hplc Chromatogram ◽  
Main Components ◽  
Layer Chromatography

This study clarifies the chemical constituents and botanical origin of Tetragonula sapiens Cockerell bee propolis collected from Southeast Sulawesi, Indonesia. Propolis samples and resin of Mangifera indica were extracted with 99% ethanol to obtain an ethanol extract of propolis (EEP) and an ethanol extract of M. indica resin (EEM). Column chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) were developed and used for the separation and isolation of compounds from the ether-soluble fraction. The structure of the compounds was determined by nuclear magnetic resonance (NMR) spectroscopic analysis, and their molecular weight analyzed by gas chromatography–mass spectrometry (GC–MS). The HPLC chromatogram of the EEP was then compared with the HPLC chromatogram of EEM to investigate the botanical origin of propolis. Five compounds were isolated from the EEP, and their structures were determined as mangiferolic acid, cycloartenol, ambonic acid, mangiferonic acid, and ambolic acid, which are cycloartane-type triterpenes. The characteristic peak of the HPLC chromatograms of EEP and EEM showed a similar pattern, which is that the main components of propolis were also found in M. indica resin. These results suggested that the propolis from Southeast Sulawesi was rich in cycloartane-type triterpenes, and the plant source of the propolis could be Mangifera indica (mango).

Pharmacognostic evaluation and instrumental analysis (SEM) for the standardization of Piper nigrum L., (black pepper) fruit

2019 ◽  
Vol 51 (5) ◽  
Author(s):  
Najma Shaheen ◽  
Shahlla Imam ◽  
Rafi Akhter Sultan ◽  
Safia Abidi ◽  
Iqbal Azhar ◽  
...  
Keyword(s):  
Black Pepper ◽  
Instrumental Analysis ◽  
Piper Nigrum ◽  
Pepper Fruit

scholarly journals EFFECT OF FEEDING TREATMENT WITH SOME EXTRACTS OF BLACK PEPPER ON SOME BIOLOGICAL ASPECTS OF COTTON LEAF WORM

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
AL-Khazraji & et al.
Keyword(s):  
Ethanol Extract ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Cotton Leaf ◽  
Food Treatment ◽  
Malva Sylvestris ◽  
Effect Of Food ◽  
Oil Extract ◽  
Biological Aspects ◽  
Effect Of Feeding

This research was conducted to study the effect of food treatment leaves of Malva sylvestris with ethanol ,ethyl acetate, hexane and oil extract of  black pepper fruits Piper nigrum on some biological aspects of cotton leaf worm Spodoptera littoralis by using the concentrations of 1.25, 2.5 and 5% of each extract, The results of feeding treatments of second larvae instar showed an increase in mortality percentages which reached 100% at the all extracts when using 5% concentrate. used in the test, while the highest mortality reached to 26.7% when using ethanol extract at 5% concentrate for sixth larvae instar fed on treated leaves for 24 hrs., it was also found that the period of larvae growth and pupae were prolonged by ethanol extract which reached to 33 days at the conc. 2.5% for the second larvae instar while reached 25 days in control, and pupae growth reached to 14 days at the same conc. while reached 10.3 days in control.

scholarly journals Performance, diversity analysis and character association of black pepper (Piper nigrum L.) accessions in the high altitude of Idukki district, Kerala

1970 ◽  
pp. 17
Author(s):  
T T Preethy, T S Aswathy, T Sathyan, M K Dhanya, M Murugan
Keyword(s):  
High Altitude ◽  
Growth Parameters ◽  
Dry Weight ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Diversity Analysis ◽  
Genotypic Correlation ◽  
Fresh Weight ◽  
Berry Weight ◽  
Character Association

The experiment was conducted to evaluate black pepper accessions for growth parameters, yield attributing characters and yield. Out of the ten accessions tested, Karimunda recorded the highest fresh (1.61 kg) and dry (508.7 g) yield of berries plant-1. Fresh weight showed significant positive genotypic correlation to dry weight and while negative correlated to 100 berry volume, 100 berry weight and number of berries spike-1. Hence, selection based on number of berries spike-1, 100 berry volume and 100 berry weight may not lead to the high yielding black pepper variety. The results showed that Karimunda is the most suitable black pepper variety for high altitude areas of Idukki district.

scholarly journals Phytochemistry and therapeutic potential of black pepper [Piper nigrum (L.)] essential oil and piperine: a review

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Kaliyaperumal Ashokkumar ◽  
Muthusamy Murugan ◽  
M. K. Dhanya ◽  
Arjun Pandian ◽  
Thomas D. Warkentin
Keyword(s):  
Animal Models ◽  
Essential Oil ◽  
Therapeutic Potential ◽  
Biological Activities ◽  
Biological Effects ◽  
Chemical Constituents ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Bioactive Constituents ◽  
Intermittent Fever

Abstract Background Black pepper [Piper nigrum (L.), Family: Piperaceae] is used traditionally for the treatment of various diseases including; cough, cold, dyspnea throat diseases, intermittent fever, dysentery, stomachache, worms and piles. The pharmacological potential of black pepper is due to the presence of metabolites like phenolic compounds, alkaloids, flavonoids, carotenoids, terpenoids, etc. The multipurpose use of black pepper dried seeds has several other beneficial health effects that also received in the light of traditional as well as current medicine perspectives. The review aims to discuss the botany, phytochemical constituents, and pharmacological properties of piperine and black pepper essential oil (BPEO). Results Phytochemical analyses have described the main chemical constituents of black pepper, including carbohydrates, proteins, calcium, magnesium, potassium, iron, vitamin C, tannins, flavonoids and carotenoids. The volatile oil content ranges from 0.4 to 7 % in dried berries. The major constituents of BPEO are sabinene, 3-carene, D-limonene, α-pinene, caryophyllene, β-phellandrene, α-phellandrene, α-thujene, and β-bisabolene. Additionally, piperine is the naturally occurring and principal bioactive alkaloid constituent of black pepper owing to its potential therapeutic properties, including cerebral brain functioning and increased nutrient absorption. The BPEO has several biological roles, including antioxidant, anti-inflammatory, anticancer, anti-obesity, antidepressant, antidiabetic, antimicrobial, gastroprotective, and insecticidal activities. Conclusions This review examines and presents the appropriate evidence on black pepper and its traditional uses as well as biological activities of BPEO and piperine. Although several previous reports showed diverse biological effects for piperine and bioactive constitutes of BPEO. Thus, minimal investigations were conducted using animal models, and many of these studies also lacked appropriate experimental setting like doses, control details. Hence, future studies are necessary to understand the mechanism of piperine, BPEO, bioactive constituents and their effects upon their use by animal models and humans with the proper experimental procedure which we can facilitate the protection of human health from several diseases.

scholarly journals Studying the Effectiveness of Some Plant Powders on the Biological Activities of the Confused Flour Beetle Tribolium confusum du Val (Coleoptera: Tenebrio-nidae).

2020 ◽  
Vol 35 (2) ◽  
pp. 166-175
Author(s):  
Nagat Ali Abuelnnor
Keyword(s):  
Biological Activities ◽  
Black Pepper ◽  
Tribolium Confusum ◽  
Piper Nigrum ◽  
Flour Beetle ◽  
Pepper Fruit ◽  
Plant Powders ◽  
Leaf Powder ◽  
Myristica Fragrans Houtt ◽  
Confused Flour Beetle

This study aims to test the biological effect of three plant powders of (black pepper fruit Piper nigrum L., nutmeg seeds Myristica fragrans Houtt and bay leaf Laurus nobilis L.) when mixed with wheat flour at concentrations of; 0.2, 0.5, 0.75, 1 and 1.5 % (w/w) against the confused flour beetle Tribolium confusum. Results showed that the black pepper fruit caused a decrease in the production of larvae that reached 65.36%, while the nutmeg seeds powder caused a decrease of 50.93% compared with the bay leaf powder, which caused a decrease of 38.20%. The results also showed a decrease in the first generation progeny (F1) that reached 90.75% and 75.34% due to the treatment with the black pepper fruit and the nutmeg seeds powders respectively. However, the bay leaf powder was less effective, with only a decrease of 30.13%.

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