The Effect Extract Bioactive Compounds Seaweed Codium Sp On Total Hemocyte Count (THC) OF Tiger shrimp (Penaeus monodon)

2021 ◽  
Vol 11 (2) ◽  
pp. 94-100
Author(s):  
Siswati Hilal Anshary ◽  
Andi Parenrengi
Keyword(s):  
Bioactive Compounds ◽  
Penaeus Monodon ◽  
Tiger Shrimp ◽  
Total Hemocyte Count ◽  
Hemocyte Count

scholarly journals RESPONS IMUN UDANG WINDU Penaeus monodon TERHADAP VAKSIN dsRNA VP-24 PADA DOSIS BERBEDA

2018 ◽  
Vol 13 (1) ◽  
pp. 77
Author(s):  
Sri Redjeki Hesti Mulyaningrum ◽  
Andi Parenrengi ◽  
Bunga Rante Tampangallo ◽  
Ike Trismawanti
Keyword(s):  
Immune Response ◽  
Survival Rate ◽  
Saline Solution ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Injection Method ◽  
Tiger Shrimp ◽  
Total Hemocyte Count ◽  
Hemocyte Count

Peningkatan produksi udang windu Penaeus monodon terus diupayakan, salah satunya dengan peningkatan respons imun udang terhadap infeksi penyakit WSSV. Penelitian ini bertujuan untuk mengetahui respons imun udang terhadap pemberian vaksin dsRNA VP-24 pada berbagai dosis. Konstruksi vaksin dsRNA VP-24 dilakukan menggunakan Megascript kit dengan DNA genom VP-24 sebagai template. Vaksinasi dilakukan dengan metode injeksi pada udang windu yang berukuran rata-rata 15,88 ± 3,50 g; dosis vaksin yang diujikan adalah 0,02 µg; 0,2 µg; 2 µg; dan sebagai kontrol adalah udang yang tidak diberi vaksin. Penelitian terdiri atas empat perlakuan dosis vaksin dengan masing-masing dua ulangan dan dipelihara selama lima hari. Uji tantang dilakukan selama enam hari dengan menginjeksi virus WSSV dalam saline solution (1:3 v/v). Pengamatan terhadap sintasan udang windu dilakukan setiap hari, sedangkan penghitungan total hemocyte (THC) dan ProPO diamati pada hari I, III, dan VI setelah diinfeksi WSSV. Pada akhir pengujian dilakukan pengambilan jaringan hepatopankreas untuk analisis histopatologi. Analisis data dilakukan secara statistik dengan analisis ragam (ANOVA). Hasil yang diperoleh memperlihatkan bahwa injeksi vaksin dsRNA VP-24 dengan dosis 0,2 µg memiliki pengaruh yang signifikan terhadap sintasan dan respons imun udang windu (P<0,05). Vaksin dsRNA VP-24 dengan dosis 0,2 µg mampu memberikan sintasan udang windu P. monodon sebesar 65% dan meningkatkan respons imun udang dengan THC (1.550 x 10t sel/mL) dan ProPO (0,042 Abs).One of the efforts to increase the production of tiger shrimp Penaeus monodon is increasing the immune response against WSSV disease. This study aims to evaluate shrimp immune response to dsRNA VP-24 vaccination at various doses. The construction of dsRNA VP-24 vaccine was performed using Megascript kit with the VP-24 DNA genome as a template. The vaccination was done by injection method on shrimp sized 15.88 ± 3.50 g. The tested vaccine doses (treatments) were 0.02 ¼g; 0.2 ¼g; 2 ¼g; and unvaccinated shrimp as the control. The study consisted of four treatments of vaccine doses with two replicates for each treatment. The challenge test was performed by injecting the WSSV virus in saline solution (1:3 v/v). The observation on shrimp survival rate was done daily, while the total hemocyte count (THC) and ProPO observation were performed on the 1st day, 3rd day, and 6th day after WSSV infection. At the end of the experiment, samplings of hepatopancreas for analysis were performed. Data were statistically analyzed using ANOVA. The present study indicated that the injection of 0.2 ¼ g dsRNA VP-24 vaccine had a significant effect on the survival rate and immune response of shrimp (P<0.05). The dose of 0.2 ¼g dsRNA VP-24 had resulted in 65% of survival rate and increased immune response of P. monodon with THC (1,550 x 10t cell/mL) and ProPO (0.042 Abs).

scholarly journals THE EFFECT OF MARINE SPONGE Aaptos aaptos EXTRACT IN VIBRIOSIS TREATMENT OF BLACK TIGER SHRIMP Penaeus monodon LARVAE

2014 ◽  
Vol 9 (2) ◽  
pp. 103
Author(s):  
Rosmiati Rosmiati ◽  
Najiah Musa ◽  
Habsah Mohammad ◽  
Tengku Sifzizul Tengku Muhammad ◽  
Andi Parenrengi ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Marine Sponge ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Toxicity Study ◽  
Histological Observation ◽  
Black Tiger Shrimp ◽  
Butanol Extract ◽  
Tiger Shrimp ◽  
Bioactive Potential

Black tiger shrimp Penaeus monodon post larvae were challenged with Vibrio harveyi and butanol extract of selected marine sponge Aaptos aaptos to determine its antibacterial bioactive potential in vibriosis treatment. Based on the preliminary toxicity study, the A. aaptos butanol extract with concentrations of 31.25, 62.5, and 125 mg/L were selected in the study. Black tiger shrimp post larvae were challenged with V. harveyi at 107 cfu/mL and immersed A. aaptos butanol extract with the concentration of 125 mg/L showed significantly in (P<0.05) decrease mortality of the post larvae treated. Besides at this concentration, V. harveyi population in the rearing water and the post larvae treated decreased compared to control (untreated post larvae). Histological observation indicated that there was no changing on hepatopancreas of the black tiger shrimp post larvae. Based on this result, it is suggested that the butanol extract of A. aaptos is a potential bioactive compounds source in the treatment of vibriosis which may replaced the current antibiotics application.

Acute toxicity, accumulation and sublethal effects of four neonicotinoids on juvenile Black Tiger Shrimp (Penaeus monodon)

Chemosphere ◽  
2021 ◽  
Vol 275 ◽  
pp. 129918
Author(s):  
Peter Butcherine ◽  
Brendan P. Kelaher ◽  
Matthew D. Taylor ◽  
Corinne Lawson ◽  
Kirsten Benkendorff
Keyword(s):  
Acute Toxicity ◽  
Sublethal Effects ◽  
Penaeus Monodon ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp

Characterization and functional analysis of fibrinogen-related protein (FreP) in the black tiger shrimp, Penaeus monodon

2021 ◽  
Vol 109 ◽  
pp. 87-96
Author(s):  
Prawit Oangkhana ◽  
Piti Amparyup ◽  
Anchalee Tassanakajon ◽  
Elumalai Preetham ◽  
Ratree Wongpanya
Keyword(s):  
Functional Analysis ◽  
Penaeus Monodon ◽  
Related Protein ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp

A key gene of the RNA interference pathway in the black tiger shrimp, Penaeus monodon: Identification and functional characterisation of Dicer-1

2008 ◽  
Vol 24 (2) ◽  
pp. 223-233 ◽  
Author(s):  
Jianguo Su ◽  
Dang T.H. Oanh ◽  
Russell E. Lyons ◽  
Lisa Leeton ◽  
Marielle C.W. van Hulten ◽  
...  
Keyword(s):  
Rna Interference ◽  
Penaeus Monodon ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp ◽  
Functional Characterisation

Unique lesions and viral-like particles found in growth retarded black tiger shrimp Penaeus monodon from East Africa

Aquaculture ◽  
2006 ◽  
Vol 253 (1-4) ◽  
pp. 197-203 ◽  
Author(s):  
Gun Anantasomboon ◽  
Siriporn Sriurairatana ◽  
Timothy W. Flegel ◽  
Boonsirm Withyachumnarnkul
Keyword(s):  
East Africa ◽  
Penaeus Monodon ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp

Identification of reproduction-related proteins and characterization of proteasome alpha 3 and proteasome beta 6 cDNAs in testes of the giant tiger shrimp Penaeus monodon

2012 ◽  
Vol 355 (1) ◽  
pp. 143-152 ◽  
Author(s):  
Sirawut Klinbunga ◽  
Sasithorn Petkorn ◽  
Suthathip Kittisenachai ◽  
Narumon Phaonakrop ◽  
Sittiruk Roytrakul ◽  
...  
Keyword(s):  
Penaeus Monodon ◽  
Tiger Shrimp ◽  
Related Proteins
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