Assessment of novel EMS-induced genetic variability in rapeseed (Brassica napus L.) and selection of promising mutants

The purpose of this work was to research the morphological characteristics of monofloral bee pollen obtained from Brassica napus L. The formulated goals were: determine the period of bee pollen receiving; implement the selection of bee pollen samples during blooming period of species; establish the monoflorality ratio of the total pollen collection; explore the morphometric indicators of bee pollen by the parameters of pollen lump, shaping level and weight. Bee pollen collection was carried out in Skvyra district of Kyiv region from local population of Apis mellifera L. families. Morphometric measurements were performed in the Institute of Biodiversity Conservation and Biosafety, Slovak University of Agriculture in Nitra. Here were determined the amount of bee pollen per one day brought by one family: from 27.04 to 03.05.16 is 50.8 ± 2.25 g; from 04.05 to 06.05.16 – 100.0 ± 3.33 g; from 07.05 to 17.05.16 – 270.7 ± 11.79 g; from 18.05 to 22.05.16 – 100.5 ± 3.028 g; from 23.05 – 25.05.16 – 39.8 ± 2.74 g. Here were defined the morphological parameters of bee pollen lump from B. napus: length is in the range from 3.40 ± 0.061 mm to 3.66 ± 0.048 mm; width is in the range from 2.88 ± 0.059 mm to 3.26 ± 0.067 mm; weight is in the range from 8.65 ± 0.317 mg to 11.31 ± 0.241 mg; shaping level of bee pollen is in the range 4.62 – 4.96 points. The total monoflorality ratio constitutes from 79.94 ± 0.619 % to 98.00 ± 0.202 %. The obtained results confirmed that the activity of bee flying depends on the blooming period and intensity of the nectar secretion, on the collection of protein feed from B. napus. Pollen lumps from Brassica napus L. bee pollen were large, dense, regular shape without splits, which specified the possibility of its use for the producing on a commercial scale.

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Marker assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value

PLoS ONE ◽

10.1371/journal.pone.0233959 ◽

2020 ◽

Vol 15 (6) ◽

pp. e0233959 ◽

Cited By ~ 1

Author(s):

Stanisław Spasibionek ◽

Katarzyna Mikołajczyk ◽

Hanna Ćwiek–Kupczyńska ◽

Teresa Piętka ◽

Krystyna Krótka ◽

...

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Marker Assisted Selection ◽

Inbred Lines ◽

Winter Oilseed Rape ◽

Brassica Napus L ◽

High Oleic ◽

Low Linolenic ◽

Selection Of

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Genetic Variability, Correlation and Path Coefficient Analysis in Advanced Generation of Brassica napus L.

Journal of Scientific Research and Reports ◽

10.9734/jsrr/2019/v22i330090 ◽

2019 ◽

pp. 1-12

Author(s):

Md. Nur-E-Nabi ◽

Md. Ehsanul Haq ◽

Montasir Ahmed ◽

Md. Monir Hossain ◽

Md. Shefat-al-Maruf ◽

...

Keyword(s):

Brassica Napus ◽

Genetic Variability ◽

Plant Height ◽

Seed Yield ◽

Coefficient Of Variation ◽

Yield Potential ◽

Path Coefficient ◽

Path Coefficient Analysis ◽

Brassica Napus L ◽

Positive Direct

The present study was conducted involving 62 F3 genotypes of Brassica napus L. at the experimental farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh to ponder the genetic variability, phenotypic, genotypic and environmental coefficient of variation, heritability and genetic advance, correlation, path coefficient and genetic diversity analysis in a randomized complete block design (RCBD) with sixty-two genotypes (treatments) with three replications during November 2014 to February 2015. The investigations aimed to select the best segregating genotypes for the yield improvement of Brussica napus (rapeseed). Analysis of variance indicated that the genotypes were found significantly different for all the characters considered. The relative phenotypic coefficient of variation (PCV) was higher than the genotypic coefficient of variation (GCV) for all the traits investigated. The high GCV value was observed for the number of siliqua per plant (NSP), plant height (PH), silique length (SL), number of seed per silique (NSS) and seed yield per plant (SYP) indicated high broad sense heritability. The significant positive correlation with seed yield per plant (SYP) was found in plant height (PH) (0.368**), the number of primary branches per plant (NPB) (0.332**), number of secondary branches per plant (NSB) (0.382**), number of silique per plant (NSP) (0.549**), and siliqua length (SL) (0.037**). The results of path coefficient analysis uncovered that plant height (PH) (0.582), days to 50% flowering (50F) (0.390), days to maturity (DM) (0.575), number of primary branches per plant (NPB) (0.678), number of secondary branches per plant (NSB) (0.182),and thousand seed weight (TSW) (0.289) had a positive direct impact on seed yield per plant (SYP) and thus it was concluded that these traits could be exploited for the enhancement of yield potential of rapeseed. This study showed that based on the agronomic performance execution, genotypes G8, G14, G19, G21, G47, and G55 might be proposed for future hybridization program in Bangladesh and this could help rapeseed breeders to upgrade their breeding activities.

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Early in vitro selection of winter oilseed rape (Brassica napus L.) plants with the fertility restorer gene for CMS Shaan 2A via non-destructive molecular analysis of microspore-derived embryos

Czech Journal of Genetics and Plant Breeding ◽

10.17221/94/2018-cjgpb ◽

2019 ◽

Vol 55 (No. 4) ◽

pp. 162-165

Author(s):

Miroslav Klíma ◽

Eva Jozová ◽

Irena Jelínková ◽

Vratislav Kučera ◽

Shengwu Hu ◽

...

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

In Vitro Selection ◽

Restorer Gene ◽

Brassica Napus L ◽

Rf Gene ◽

Dh Lines ◽

Non Destructive ◽

Selection Of

Cotyledons of 201 microspore-derived oilseed rape (Brassica napus) embryos were analysed for the presence of the cytoplasmic male sterility Shaan 2A restorer gene using the primer pair 3F/4R. In total, 172 plants regenerated and were grown to the generative stage. Among phenotypically non-haploid flowering plants, there was a broad spectrum of different flower types from normally developed flowers to flowers with short anthers, stamens, or deformed flowers with atypically developed floral organs, often with traces of pollen. For this reason, only 115 typically fertile or sterile accessions were selected for further phenotype-genotype comparisons. In 14 plants (12.2%) the phenotype did not match the genotype: two plants were marked as fertile, but without the Rf gene. Vice versa, in 12 sterile plants the Rf gene was determined. Nevertheless, the two fertile plants did not produce any pods after self-pollination. In total, 47 doubled haploid (DH) lines with good seed yield, carrying the Rf gene were harvested.

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