scholarly journals Restorative effect of Azadirachta indica against fenvalerate induced haematological and biochemical toxicity in a freshwater fish Clarias batrachus

2020 ◽  
Vol 12 (4) ◽  
pp. 491-496
Author(s):  
Pratibha Kumari ◽  
Sanjay Kumar Jigyasu ◽  
Dilip Kumar Paul

Pesticide use in recent times has increased many folds to enhance the yield of crops. The water bodies like ponds and others are extensively used for the cultivation of fishes. Since these fishes are a good source of protein and nutritious value, hence are consumed by humans. These pesticide-contaminated fishes are consumed by the human, causing health hazards. The objective of the present research work was to know the bio remedial effect of Azadirachta indica on fenvalerate induced toxicity in a freshwater air-breathing catfish "Mangur" known as Clarias batrachus ( Linnaeus). The fishes were treated with 1/3rd of LC50, i.e. 0.92 ppm of a pyrethroid pesticide fenvalerate and observed the fishes for 96 hours. Thereafter, the aqueous leaf extract of Azadirachta indica was administered orally, daily by gastric intubation method at the dose of 100 mg/Kg body weight per day for 15 days. The study revealed that, after the exposure of 0.92 ppm of fenvalerate, there was a significant decrease in RBC count from 2.76 to 2.14 X (106) (mm-3), Total leukocyte count from18.10 to 16.30 X (103) (mm-3), , packed ell volume from 24 % to 16% , haemoglobin from 6.89 g/100 ml to 4.23 g/100ml of blood whereas increase in the level of serum glucose and cholesterol level from 50.33 mg/dl to 103.73 mg/dl and 168.52 g/dl to 248.99 g/dl respectively. At the same time, serum protein level decreased from  3.16g/dl to2.52 g/dl due to pesticide in the experimental fishes.   But, after the administration of aqueous leaf extract of Azadirachta indica there was significant normalisation in the test parameters of the fishes. The study indicated that neem leaves possessed restorative effect against fenvalerate induced toxicity in Clarias batrachus.

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Voravuth Somsak ◽  
Sukanya Chachiyo ◽  
Ubonwan Jaihan ◽  
Somrudee Nakinchat

Malaria is a major public health problem in the world because it can cause of death in patients. Malaria-associated renal injury is associated with 45% of mortality in adult patients hospitalized with severe form of the disease. Therefore, new plant extracts to protect against renal injury induced by malaria infection are urgently needed. In this study, we investigated the protective effect of aqueous crude extract ofAzadirachta indica(neem) leaves on renal injury induced byPlasmodium bergheiANKA infection in mice. ICR mice were injected intraperitoneally with 1 × 107parasitized erythrocytes of PbANKA, and neem extracts (500, 1,000, and 2,000 mg/kg) were given orally for 4 consecutive days. Plasma blood urea nitrogen (BUN) and creatinine levels were subsequently measured. Malaria-induced renal injury was evidenced as marked increases of BUN and creatinine levels. However, the oral administration of neem leaf extract to PbANKA infected mice for 4 days brought back BUN and creatinine levels to near normalcy, and the highest activity was observed at doses of 1,000 and 2,000 mg/kg. Additionally, no toxic effects were found in normal mice treated with this extract. Hence, neem leaf extract can be considered a potential candidate for protection against renal injury induced by malaria.


2011 ◽  
Vol 12 (1) ◽  
Author(s):  
Vânia Campos SILVA ◽  
Delineide Pereira GOMES ◽  
Ceália Cristine SANTOS

Este estudo teve o objetivo de avaliar o efeito do extrato aquoso de folhas de nim sobre a germinação e incidência de fungos em sementes de três cultivares (Serrinha, BR-17 e Maranhão) de feijão-caupi. Folhas de nim foram secas, trituradas e preparadas diluições de 0,5; 1,0; 2,0 e 4,0 g dm-3 e testemunha. Avaliou-se a incidência de fungos, pelo teste de papel de filtro e a germinação de acordo com as Regras para Análise de Sementes. Nas três cultivares analisadas, observouse redução na incidência de Aspergillus sp e Fusarium sp. Em relação à influência dos extratos das folhas de nim sobre a germinação das sementes, observou efeito significativo do extrato na cultivar Maranhão, onde todas as concentrações diferiram da testemunha e proporcionaram aumento considerável na porcentagem de plântulas normais. Conclui- se que o extrato de folhas de nim foi efetivo no controle de Aspergillus sp, Fusarium sp, Phoma sp e Macrophomina phaseolina em diferentes concentrações nos diferentes cultivares e a germinação das sementes foi estimulada para a cultivar Maranhão Abstract This study aimed to evaluate the effect of aqueous extract of neem on germination and fungi incidence on seeds of three cultivars (Serrinha, BR 17 and Maranhão) of cowpea. Neem leaves were dryed, crushed and prepared dilutions of 0.5; 1.0; 2.0, 4.0 g dm-3 and control. The fungi incidence was evaluated by the test filter paper and germination according to the Rules for Seeds Testing (Regras para Análise de Sementes). In the three cultivars analyzed, reduction in the incidence of Aspergillus sp and Fusarium sp was observed. In relation to the influence of extracts of neem leaves on seed germination, significant effect of extract in Maranhão cultivar was observed, where all concentrations differed from the control, and propovided a considerable increase in the percentage of normal seedlings. It was concluded that the leaf extract of neem was effective in controlling Aspergillus sp, Fusarium sp , Phoma sp and Macrophomina phaseolina at different concentrations in different cultivars and seed germination was stimulated for the Maranhão cultivar. 


2019 ◽  
Vol 6 (9) ◽  
pp. 3359-3367 ◽  
Author(s):  
Emmanuel Obasi ◽  
Kizito Iheanacho ◽  
Ngwu Nwachukwu ◽  
Nc Agha ◽  
Paul Chidoka Chikezie

Background: The present study evaluated the effect of phenolic aqueous leaf extract of Vitex doniana on body weight, serum glucose and oxidative stress parameters in diabetes mellitus (DM) rats. Methods: DM was induced in rats by intraperitoneal injection of 100 mg/kg alloxan monohydrate in phosphate buffered saline (PBS; pH = 7.4). A total of 36 adult male Wister albino rats were divided into 6 groups of 6 rats. The groups consist of normal untreated rats, untreated DM rats, DM rats treated with 500 mg/kg dimethylguanide (MetforminTM) and DM rats treated with 100, 200 and 400 mg/kg body weight of phenolic aqueous leaf extract of Vitex doniana. The rats were treated for 28 days. Serum glucose, malondialdehyde (MDA), glutathione (GSH) and ascorbic acid concentrations, and serum superoxide dismutase (SOD) and catalase (CAT) activities were measured using standard methods. The changes in body weight was also measured. Results: The results showed a significant reduction (p < 0.05) in serum glucose and MDA concentration, whereas serum SOD, CAT activities, as well as GSH and ascorbic acid concentration were significantly elevated (p < 0.05) in treated DM groups in a dose dependent manner. Conclusion: The results of the present study indicated that phenolic aqueous leaf extract of V. doniana promoted increased body weight, ameliorated DM and alleviated reduced antioxidant activities in alloxan-induced DM rats. Phenolic aqueous leaf extract of V. doniana could serve as a potential natural and safe remedy for the management of DM.  


2016 ◽  
Vol 9 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Shakeel Ahmed ◽  
Saifullah ◽  
Mudasir Ahmad ◽  
Babu Lal Swami ◽  
Saiqa Ikram

2021 ◽  
Vol 104 (2) ◽  
pp. 003685042110121
Author(s):  
Kashan Khan ◽  
Saleem Javed

A wide variety of methods have synthesized silver nanoparticles (Ag-NPs) in the recent past; however, biological methods have attracted much attention over the traditional chemical synthesis method due to being non-hazardous and eco-friendly. Here, a detailed and systemic study was performed to compare two different synthesis routes for Ag-NPs, that is, the chemical and the biological; their possible outcomes have also been described. Ag-NPs were synthesized chemically (cAg-NPs) using a chemical reductant and biologically (bAg-NPs) by using aqueous leaf extract of Azadirachta indica (neem). The synthesized nanoparticles were characterized using UV-visible spectrophotometry, FT-IR, EDX, and TEM. The average particle sizes (APS) of cAg-NPs were found to be 8 and 13 nm and of bAg-NPs to be 19 and 43 nm under different AgNO3 concentrations. The antimicrobial tests of differently sized NPs were performed against Escherichia coli (Gram −ve) and S taphylococcus aureus (Gram +ve). The results revealed that bAg-NPs of APS 43 nm were highly antimicrobial against both the tested bacterial stains followed by cAg-NPs of 8 nm. We found the effect of cAg-NPs to be size-dependent, whereas bAg-NPs showed a more significant antimicrobial effect than cAg-NPs.


AGROINTEK ◽  
2021 ◽  
Vol 15 (2) ◽  
pp. 649-657
Author(s):  
Supriyanto Supriyanto ◽  
Simon Bambang Widjanarko ◽  
Muhaimin Rifa'i ◽  
Yunianta Yunianta

The neem plant (Azadirachta indica) is a herb with the potential as a source of antioxidants. This study aimed to identify compounds that exist in the neem leaf and determine their antioxidant activity. Neem leaf extract was collected by using 80% methanol.  Furthermore, methanol extracts from neem leaves with the highest antioxidant activity were partitioned with n-hexane, ethyl acetate, and water. This research were analyzed by partition for antioxidant activity, and DPPH (1,1-diphenyl-2-pycrilhydrazil) and the half-maximal inhibitory concentration (IC50) values were determined. The results showed that the ethyl acetate partition had the highest antioxidant activity, with IC50 values of 1.004 µg/ml. Neem leaf extract has the potential for a use as an herbal medicine in the treatment of various diseases..


2020 ◽  
Vol 17 (2) ◽  
Author(s):  
R. Roy ◽  
M. H. Hasan ◽  
F. B. Aziz ◽  
R. Islam ◽  
S. Sarkar

Background: Coccidiosis is recognized as the parasitic disease with the greatest economic impact on poultry industries worldwide. Sonali chicken are commonly affected by coccidiosis in Bangladesh. Recently, the concerns about possible drug resistance have aroused great caution in the usage of drug in the animal industry. As an alternative herbal extracts are used and Neem leaves (Azadirachta indica) have anti-coccidial property. This study was conducted to evaluate the comparative efficacy of Neem leaves suspension and toltrazuril on coccidial load, blood constituents, growth performance of Sonali chicken infected with Eimeria tenella. Methods: The study was conducted in the laboratory and experimental shed under the department of physiology and pharmacology, HSTU, Dinajpur during, 2nd January to 3rd February, 2019. Eighty (80) Sonali chicken of seven days old were randomly divided into four groups named T0, T1 T2 and T3 and each group contained 20 birds. All groups were supplied E. tenella orally except T0 group and after 3 days T2 group was treated with Neem leaves suspension (5%) for 15 days and T3 groups was treated with toltrazuril (topzuril ®) solution (1 ml/lit) for 2 days. Results: Results showed that protozoal load decreased significantly (P<0.01) in T2 & T3 groups whereas increased significantly (P<0.01) in T0 and T1 group. The total erythrocyte count and hemoglobin in different treatment groups were similar and the differences were non-significant (P>0.05) statistically except total leukocyte count (TLC). The body weight of T0, T1, T2 and T3 group at day 30 were 432.65, 469.81, 548.81, 489.22 gm respectively which is statistically significant (P<0.01) and highest body weight gain was recorded from Neem leaves suspension treated group. Conclusion: Neem leaves suspension have significant effects on protozoal load, body weight and no significant effect on haematological parameters except TLC.


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