Differential Effects in Vivo of Thyroid Hormone on the Expression of Surfactant Phospholipid, Surfactant Protein mRNA and Antioxidant Enzyme mRNA in Fetal Rat Lung

1998 ◽  
Vol 24 (5) ◽  
pp. 641-657 ◽  
Author(s):  
Sujatha M. Ramadurai ◽  
Heber C. Nielsen ◽  
Youwei Chen ◽  
Dimitrios Hatzis ◽  
Llene R. S. Sosenko
Keyword(s):  
Thyroid Hormone ◽  
Antioxidant Enzyme ◽  
Surfactant Protein ◽  
Rat Lung ◽  
Differential Effects ◽  
Fetal Rat ◽  
Fetal Rat Lung

Effects of Betamethasone and Thyroid Hormone on Fetal Rat Lung Maturation in vivo

1992 ◽  
Vol 18 (1-2) ◽  
pp. 14-19 ◽  
Author(s):  
Fernando R. Moya ◽  
Ignacio Sanchez ◽  
Deborah Baudy
Keyword(s):  
Thyroid Hormone ◽  
Rat Lung ◽  
Lung Maturation ◽  
Fetal Rat ◽  
Fetal Rat Lung

scholarly journals Glucocorticoid-Thyroid Hormone Interactions in Fetal Rat Lung

1984 ◽  
Vol 18 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Jan Gross ◽  
Diane W Dynia ◽  
Christine M Wilson ◽  
Linda D Ingleson ◽  
Ira H Gewolb ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Rat Lung ◽  
Hormone Interactions ◽  
Fetal Rat ◽  
Fetal Rat Lung

Retinoic acid increases surfactant protein mRNA in fetal rat lung in culture

1996 ◽  
Vol 271 (5) ◽  
pp. L862-L868 ◽  
Author(s):  
C. W. Bogue ◽  
H. C. Jacobs ◽  
D. W. Dynia ◽  
C. M. Wilson ◽  
I. Gross
Keyword(s):  
Retinoic Acid ◽  
Dose Response ◽  
Consensus Sequence ◽  
Surfactant Protein ◽  
Rat Lung ◽  
Response Element ◽  
Response Characteristics ◽  
Receptor Complexes ◽  
Fetal Rat ◽  
Fetal Rat Lung

Retinoic acid has both early or immediate (within hours) and late (after days) effects on gene expression. We studied the early effects of retinoic acid on the surfactant protein (SP) genes. Exposure of fetal rat lung explants to all trans-retinoic acid for 4 h resulted in a significant dose-dependent increase in SP-A, -B, and -C mRNA with markedly different dose-response characteristics. The maximal (2.5x) increase in SP-A mRNA was observed with 10(-10) M retinoic acid, whereas treatment with 10(-5) M resulted in a tendency to decreased levels. In contrast, maximal stimulation of SP-C (6x) was noted at 10(-5) M retinoic acid and that of SP-B (2x) at 10(-7) to 10(-5) M retinoic acid. Similar differences in the dose-response characteristics of SP-A and SP-C were observed with 9-cis-retinoic acid. A retinoic acid response element consensus sequence was identified in the rat SP-A gene; we hypothesize that retinoic acid-receptor complexes act directly on the SP-A gene via this response element.

An organ culture model for study of biochemical development of fetal rat lung

1978 ◽  
Vol 45 (3) ◽  
pp. 355-362 ◽  
Author(s):  
I. Gross ◽  
G. J. Smith ◽  
W. M. Maniscalco ◽  
M. R. Czajka ◽  
C. M. Wilson ◽  
...  
Keyword(s):  
Organ Culture ◽  
Late Gestation ◽  
In Vivo Studies ◽  
Morphological Development ◽  
Rat Lung ◽  
Fetal Rat ◽  
Culture Model ◽  
Fetal Rat Lung

We have developed a short-term organ culture model for the study of the biochemical and morphological development of late gestation fetal rat lung. Explants (1 mm3) of 19-day lung were cultured in an oxygen enriched environment in the presence of synthetic serum-free medium for 3 days. Morphological maturation continued in culture. The rate of incorporation of choline into disaturated phosphatidylcholine and the content of this phospholipid in the explants increased in vitro in a pattern very similar to that which occurs in vivo. The activities of choline kinase and cholinephosphotransferase were also similar in cultured lung and in vivo. Studies of glucose oxidation to CO2 provided additional evidence that the explants remained viable in culture. The explants retained the sensitivity of fetal lung to hormonal action. This was demonstrated by the stimulation of choline incorporation into phospholipid by cyclic AMP and an increase in the glycogen content after exposure to insulin.

Butyrate modulates surfactant protein mRNA in fetal rat lung by altering mRNA transcription and stability

1994 ◽  
Vol 267 (1) ◽  
pp. L9-L15 ◽  
Author(s):  
S. M. Peterec ◽  
K. V. Nichols ◽  
D. W. Dynia ◽  
C. M. Wilson ◽  
I. Gross
Keyword(s):  
Butyric Acid ◽  
Protein A ◽  
Maternal Diabetes ◽  
Surfactant Protein ◽  
Rat Lung ◽  
Mrna Transcription ◽  
Lung Maturation ◽  
Mrna Concentration ◽  
Fetal Rat ◽  
Fetal Rat Lung

We examined the effects of Na butyrate, a known regulator of gene expression, on surfactant protein mRNA concentration, transcription, and degradation. Exposure of explants of 18-day fetal rat lung to Na butyrate resulted in a decrease in surfactant protein A (SP-A) mRNA concentration to 7% of control after 6 h and to 18% of control after 24 h. The reduction in SP-A mRNA concentration was associated with decreased mRNA transcription and stability at both these times. The effects on SP-B mRNA were similar to those on SP-A, but quantitatively less. In contrast, butyrate had a biphasic effect on SP-C mRNA concentration. There was an initial decrease to 30% of control at 6 h, followed by an increase to control levels by 24 h. Transcription of SP-C was increased at both these times, whereas degradation was enhanced at 6 h, but not at 24 h. The level of surfactant protein mRNA after butyrate treatment therefore depends on the balance between induced changes in transcription and degradation. Butyrate had no effect on gamma-actin mRNA concentration in this system. Circulating levels of butyric acid analogues are elevated in the mothers and fetuses in diabetic pregnancies. Some of these fetuses have delayed lung maturation and decreased amniotic fluid SP-A levels. We speculate that butyric acid analogues partially mediate the changes in pulmonary maturation induced by maternal diabetes.

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