Adenosine Inhibits Polymorphonuclear Leukocyte in Vitro Activation: A Possible Role as an Endogenous Calcium Entry Blocker

1985 ◽  
Vol 7 (2) ◽  
pp. 203-215 ◽  
Author(s):  
F. Laghi Pasini ◽  
P. L. Capecchi ◽  
A. Orrico ◽  
L. Ceccatelli ◽  
T. DÍ Perri
Keyword(s):  
Polymorphonuclear Leukocyte ◽  
Calcium Entry ◽  
Calcium Entry Blocker ◽  
Entry Blocker ◽  
In Vitro Activation

scholarly journals Signal transduction mechanisms involved in in vitro ram sperm capacitation

Reproduction ◽  
2006 ◽  
Vol 132 (5) ◽  
pp. 721-732 ◽  
Author(s):  
Patricia Grasa ◽  
José Álvaro Cebrián-Pérez ◽  
Teresa Muiño-Blanco
Keyword(s):  
Tyrosine Phosphorylation ◽  
Calcium Influx ◽  
Calcium Entry ◽  
Calcium Entry Blocker ◽  
Protein Tyrosine Phosphorylation ◽  
Sperm Capacitation ◽  
Protein Tyrosine ◽  
Entry Blocker ◽  
Ram Sperm

We validate the chlortetracycline (CTC) technique for the evaluation of capacitation and acrosome reaction-like changes in ram sperm, carrying out a double estimation of the acrosome status after treatment with lysophosphatidylcholine, using fluorescein isocyanate (FITC)-RCA/ethidium homodimer 1 (EthD-1) and CTC/EthD-1. Highly consistent results and a positive correlation between the results of acrosome-reacted sperm evaluated with both techniques were obtained. In this study, we evaluate the effects of ram sperm capacitation of BSA, Ca2+, NaHCO3and cAMP agonists and their influence on the associated protein tyrosine phosphorylation. We found a time-dependent increase in capacitation related to protein tyrosine phosphorylation, either in the absence or the presence of BSA. The addition of an increasing concentration of cholesterol to samples containing BSA did not influence results. The effect of bicarbonate was concentration-dependent, with a significantly lowered value of non-capacitated sperm in the presence 18 and 25 mM. The addition of extracellular calcium did not significantly increase either the proportion of capacitated sperm or the protein tyrosine phosphorylation signalling, although a significantly higher value of acrosome-reacted sperm was found in samples containing 4 mM Ca2+. cAMP agonists increased capacitated sperm and protein tyrosine phosphorylation signalling. The inhibition of protein kinase A by H-89 caused a decrease in sperm capacitation. Addition of a calcium-entry blocker (Verapamil; Sigma) did not influence results, which suggests that the calcium entry blocker was unable to inhibit the calcium influx associated with capacitation in ram sperm. Our findings might benefit our understanding of the biochemical mechanisms involved in mammalian sperm capacitation and ultimately, fertility.

In Vivo and in Vitro Evidence of an Adenosine-Mediated Mechanism of Calcium Entry Blocker Activities

Angiology ◽  
1989 ◽  
Vol 40 (3) ◽  
pp. 190-198 ◽  
Author(s):  
T. Di Perri ◽  
F. Laghi Pasini ◽  
S. Pecchi ◽  
V. De Franco ◽  
P. Damiani ◽  
...  
Keyword(s):  
Calcium Entry ◽  
Calcium Entry Blocker ◽  
Entry Blocker

Renal effects of nicardipine, a calcium entry blocker, in hypertensive type II diabetic patients with nephropathy

Diabetes ◽  
1986 ◽  
Vol 35 (11) ◽  
pp. 1206-1214 ◽  
Author(s):  
T. Baba ◽  
T. Ishizaki ◽  
Y. Ido ◽  
K. Aoyagi ◽  
S. Murabayashi ◽  
...  
Keyword(s):  
Calcium Entry ◽  
Diabetic Patients ◽  
Calcium Entry Blocker ◽  
Type Ii ◽  
Renal Effects ◽  
Entry Blocker

Selective Effect of KB-2796, a New Calcium Entry Blocker, on Cerebral Circulation

1990 ◽  
Vol 16 (3) ◽  
pp. 430-437 ◽  
Author(s):  
Toshiro Kanazawa ◽  
Tominori Morita ◽  
Kengo Harada ◽  
Takahiro Iwamoto ◽  
Hiroshi Ohtaka ◽  
...  
Keyword(s):  
Cerebral Circulation ◽  
Calcium Entry ◽  
Calcium Entry Blocker ◽  
Selective Effect ◽  
Entry Blocker

scholarly journals Effect of a New Calcium Entry Blocker, NB-818, on Delayed Neuronal Death in the Ischemic Gerbil Hippocampus.

1991 ◽  
Vol 56 (3) ◽  
pp. 279-286 ◽  
Author(s):  
Kazuo Kamei ◽  
Sonoko Tsuchida ◽  
Kazuhiro Taguchi ◽  
Masaru Nishikibe
Keyword(s):  
Neuronal Death ◽  
Calcium Entry ◽  
Delayed Neuronal Death ◽  
Calcium Entry Blocker ◽  
Entry Blocker

Suppression of Water Shift into Intracellular Space by TA-3090 (Calcium Entry Blocker) Measured with NMR

1990 ◽  
pp. 204-206
Author(s):  
M. Yamamoto ◽  
M. Haida ◽  
R. Taniguchi ◽  
K. Yazaki ◽  
D. Kurita ◽  
...  
Keyword(s):  
Calcium Entry ◽  
Calcium Entry Blocker ◽  
Intracellular Space ◽  
Water Shift ◽  
Entry Blocker
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