Flow Cytometric Detection of Minimal Residual Disease in Acute Lymphoblastic Leukemia

2003 ◽  
Vol 44 (9) ◽  
pp. 1445-1455
Author(s):  
Michael N. Dworzak ◽  
Eva R. Panzer-Grümayer
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Lymphoblastic Leukemia ◽  
Flow Cytometric ◽  
Minimal Residual

scholarly journals Improved flow cytometric detection of minimal residual disease in childhood acute lymphoblastic leukemia

Leukemia ◽  
2012 ◽  
Vol 27 (3) ◽  
pp. 635-641 ◽  
Author(s):  
B Denys ◽  
A J van der Sluijs-Gelling ◽  
C Homburg ◽  
C E van der Schoot ◽  
V de Haas ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Lymphoblastic Leukemia ◽  
Childhood Acute Lymphoblastic Leukemia ◽  
Flow Cytometric ◽  
Minimal Residual

scholarly journals Simplified flow cytometric assay to detect minimal residual disease in childhood with acute lymphoblastic leukemia

2008 ◽  
Vol 30 (4) ◽  
Author(s):  
Elizabete Delbuono ◽  
Yumi H. Maekawa ◽  
Maria do Rosário D. O. Latorre ◽  
Adriana Seber ◽  
Antonio Sergio Petrilli ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Lymphoblastic Leukemia ◽  
Flow Cytometric Assay ◽  
Flow Cytometric ◽  
Minimal Residual

Flow cytometric evidence for minimal residual disease and cytological heterogeneities in acute lymphoblastic leukemia with severe hypodiploidy

1989 ◽  
Vol 32 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Masahito Tsurusawa ◽  
Yasuhiko Kaneko ◽  
Naoyuki Katano ◽  
Makoto Niwa ◽  
Mari Ito ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Lymphoblastic Leukemia ◽  
Flow Cytometric ◽  
Minimal Residual

Clinical importance of minimal residual disease in childhood acute lymphoblastic leukemia

Blood ◽  
2000 ◽  
Vol 96 (8) ◽  
pp. 2691-2696 ◽  
Author(s):  
Elaine Coustan-Smith ◽  
Jose Sancho ◽  
Michael L. Hancock ◽  
James M. Boyett ◽  
Frederick G. Behm ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Minimal Residual Disease ◽  
Induction Therapy ◽  
Residual Disease ◽  
Lymphoblastic Leukemia ◽  
Remission Induction ◽  
Induction Phase ◽  
Continuation Therapy ◽  
Flow Cytometric ◽  
Minimal Residual

Abstract By using rapid flow cytometric techniques capable of detecting one leukemic cell in 104 normal cells, we prospectively studied minimal residual disease (MRD) in 195 children with newly diagnosed acute lymphoblastic leukemia (ALL) in clinical remission. Bone marrow aspirates (n = 629) were collected at the end of remission induction therapy and at 3 intervals thereafter. Detectable MRD (ie, ≥0.01% leukemic mononuclear cells) at each time point was associated with a higher relapse rate (P < .001); patients with high levels of MRD at the end of the induction phase (≥1%) or at week 14 of continuation therapy (≥0.1%) had a particularly poor outcome. The predictive strength of MRD remained significant even after adjusting for adverse presenting features, excluding patients at very high or very low risk of relapse from the analysis, and considering levels of peripheral blood lymphoblasts at day 7 and day 10 of induction therapy. The incidence of relapse among patients with MRD at the end of the induction phase was 68% ± 16% (SE) if they remained with MRD through week 14 of continuation therapy, compared with 7% ± 7% if MRD became undetectable (P = .035). The persistence of MRD until week 32 was highly predictive of relapse (all 4 MRD+patients relapsed vs 2 of the 8 who converted to undetectable MRD status; P = .021). Sequential monitoring of MRD by the method described here provides highly significant, independent prognostic information in children with ALL. Recent improvements in this flow cytometric assay have made it applicable to more than 90% of all new patients.

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