[Delta]-Aminolevulinic acid dehydratase activity during tomato fruit development and ripening

1994 ◽  
Author(s):  
Marios Costa Kyriacou
HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 538d-538
Author(s):  
Marios C. Kyriacou ◽  
David J. Hannapel ◽  
Richard J. Gladon

Tomato fruit ripening is characterized by a decrease in chlorophyll content and an increase in lycopene synthesis. 5-Aminolevulinic acid (ALA) dehydratase (ALAD) is the fruit committed enzyme in the chlorophyll and heme biosynthetic pathways, and it catalyzes the dimerization of two ALA molecules into porphobilinogen We have focused our attention on the potential pivotal role of ALAD in the developmental regulation of chlorophyll biosynthesis during tomato fruit growth, development, and ripening. We have standardized an assay procedure for measuring the enzymatic activity of ALAD in tomato fruit tissues. The activity of ALAD was assayed from ten days past anthesis to day 60, when fruits where void of chlorophyll. We observed a several-fold decline in ALAD activity to residual levels during fruit ontogeny. Our data also show greater ALAD activity in chlorophyllous organs (leaves, stems, immature fruits) than in nonchlorophyllous organs (roots, ripe fruits), where heme production is predominant.


1998 ◽  
Vol 1 (1) ◽  
pp. 24-25
Author(s):  
I. Elezaj ◽  
D.A. Rozhaja

In comparison with control animals, a significant negative correlation (r = 0.77 -), between lead level and the activity of daminolevulinic acid dehydratase activity in the blood of land turtle (Testudo hermanni, Gmel.) caught in five different localities from heavy polluted environment of lead and zinc smelter “Trepca” was found. The concentration ratio of lead level in blood between control animals and those from the vicinity of smelter was 10:160 % and ratio of ALA-D activity was 10:18,3 U/LE.


2020 ◽  
Author(s):  
Ricardo Bianchetti ◽  
Nicolas Bellora ◽  
Luis A de Haro ◽  
Rafael Zuccarelli ◽  
Daniele Rosado ◽  
...  

AbstractPhytochrome-mediated light and temperature perception has been shown to be a major regulator of fruit development. Furthermore, chromatin remodelling via DNA demethylation has been described as a crucial mechanism behind the fruit ripening process; however, the molecular basis underlying the triggering of this epigenetic modification remains largely unknown. Here, an integrative analyses of the methylome, siRNAome and transcriptome of tomato fruits from phyA and phyB1B2 null mutants was performed, revealing that PHYB1 and PHYB2 influences genome-wide DNA methylation during fruit development and ripening. The experimental evidence indicates that PHYB1B2 signal transduction relies on a gene expression network that includes chromatin organization factors (DNA methylases/demethylases, histone-modifying enzymes and remodelling factors) and transcriptional regulators, ultimately leading to altered mRNA profile of photosynthetic and ripening-associated genes. This new level of understanding provides insights into the orchestration of epigenetic mechanisms in response to environmental cues affecting agronomical traits in fleshy fruits.


2002 ◽  
Vol 45 (1) ◽  
pp. 85-89 ◽  
Author(s):  
V.M. Morsch ◽  
M.R.C. Schetinger ◽  
A.F. Martins ◽  
J.B.T. Rocha

Sign in / Sign up

Export Citation Format

Share Document