scholarly journals Some Characteritics of Si Target used for an Electron Microscopic Image Intensifier

Shinku ◽  
1971 ◽  
Vol 14 (5) ◽  
pp. 178-183
Author(s):  
Hideo TODOKORO ◽  
Tsutomu KONODA ◽  
Mikio ASHIKAWA
Keyword(s):  
Image Intensifier ◽  
Microscopic Image ◽  
Electron Microscopic ◽  
Electron Microscopic Image

An Efficient Adaptive Algorithm for Electron Microscopic Image Enhancement and Feature Extraction

2019 ◽  
Vol 9 (1) ◽  
pp. 1-16
Author(s):  
Vivek Arya ◽  
Vipul Sharma ◽  
Garima Arya
Keyword(s):  
Feature Extraction ◽  
Contrast Enhancement ◽  
Block Size ◽  
Sigmoid Function ◽  
Microscopic Image ◽  
Electron Microscopic ◽  
Microscopic Images ◽  
Electron Microscopic Image ◽  
Block Based

In this article, a block-based adaptive contrast enhancement algorithm has been proposed, which uses a modified sigmoid function for the enhancement and features extraction of electron microscopic images. The algorithm is based on a modified sigmoid function that adapts according to the input microscopic image statistics. For feature extraction, the contrast of the image is very important and authentic property by which this article enhances the visual quality of the image. In this work, for better contrast enhancement of image, a block based on input value, combined with a modified sigmoid function that is used as contrast enhancer provides better EMF values for a smaller block size. It provides localized contrast enhancement effects adaptively which is not possible using other existing techniques. Simulation and experimental results demonstrate that the proposed technique gives better results compared to other existing techniques when applied to electron microscopic images. After the enhancement of microscopic images of actinomycetes, various important features are shown, like coil or spiral, long filament, spore and rod shape structures. The proposed algorithm works efficiently for different dark and bright microscopic images.

scholarly journals Correlating Fluorescence Microscopy with Electron Microscopy

2004 ◽  
Vol 12 (1) ◽  
pp. 3-7
Author(s):  
Stephen W. Carmichael ◽  
Jon Charlesworth
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fluorescence Microscopy ◽  
Fluorescent Probes ◽  
Cell Biology ◽  
Microscopic Image ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Electron Microscopic Image ◽  
Auto Fluorescence

The use of fluorescent probes is becoming more and more common in cell biology. It would be useful if we were able to correlate a fluorescent structure with an electron microscopic image. The ability to definitively identify a fluorescent organelle would be very valuable. Recently, Ying Ren, Michael Kruhlak, and David Bazett-Jones devised a clever technique to correlate a structure visualized in the light microscope, even a fluorescing cell, with transmission electron microscopy (TEM).Two keys to the technique of Ren et al are the use of grids (as used in the TEM) with widely spaced grid bars and the use of Quetol as the embedding resin. The grids allow for cells to be identified between the grid bars, and in turn the bars are used to keep the cell of interest in register throughout the processing for TEM. Quetol resin was used for embedding because of its low auto fluorescence and sectioning properties. The resin also becomes soft and can be cut and easily peeled from glass coverslips when heated to 70°C.

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