scholarly journals Comparison between EPC and FHM cell lines on IHN virus susceptivility and effect of fetal bovine serum concentration on IHNV-CPE appearance.

1985 ◽  
Vol 20 (1) ◽  
pp. 69-70
Author(s):  
Teiichi NISHIMURA ◽  
Motohiko SANO ◽  
Fuyuki MOTOYAMA ◽  
Yoshikazu KANAI ◽  
Tokuo SANO
2009 ◽  
Vol 12 (9) ◽  
pp. 12-22
Author(s):  
Phuc Van Pham ◽  
Tam Thanh Nguyen ◽  
Nhung Thi Hong Vuong ◽  
Tuyet Thi Bach Duong ◽  
Ngoc Kim Phan

Mesenchymal stem cells (MSCs) can be derived from many different sources. Umbilical cord blood is a rich source of MSCs. The cryopreservation of MSCs that MSCs are still alive and differentiate into many different kinds of functional cells is very important. The aims of this research are to identify ratio of alive and dead cells as well as stemness of them after thaw. The results showed that the stemness was not affected by cryopreservative protocols or media. All cells being alive after thaw could form colonies and differentiate into adipocytes and osteoblasts. Ratio of alive and dead cells was affected very much by cryopreservative protocols and media.


Lipids ◽  
2010 ◽  
Vol 45 (3) ◽  
pp. 275-283 ◽  
Author(s):  
Ana L. Villasuso ◽  
Patricio Romero ◽  
Mariela Woelke ◽  
Patricia Moyano ◽  
Estela Machado ◽  
...  

BioTechniques ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 126-132
Author(s):  
Nan Yang ◽  
Don D Sin ◽  
Delbert R Dorscheid

Commercially available lipopolysaccharide (LPS) is commonly used in research. Although protocols for its use are well established, we experienced a loss of LPS responsiveness in our cell cultures despite no obvious experimental changes. Our cell lines were stimulated with LPS and the media quantified for LPS responsiveness via an IL-8 ELISA. We discovered that the major cause of signal loss was differences in fetal bovine serum (FBS) formulation and concentration. One FBS formulation was notably better at eliciting an IL-8 signal than the second FBS, and 10% FBS in media was better at inducing LPS responsiveness than lower concentrations. We urge researchers to be aware of inherent variations in seemingly commonplace reagents as they may be unexpected sources of inconsistencies.


2016 ◽  
Vol 17 (6) ◽  
pp. 476-483 ◽  
Author(s):  
Di Chen ◽  
Xiao-xuan Xin ◽  
Hao-cheng Qian ◽  
Zhang-yin Yu ◽  
Li-rong Shen

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