Research of carbohydrate type influence on Lonicera caerulea l. Morphogenetic potential in vitro

2020 ◽  
Vol 61 ◽  
pp. 54-60
Author(s):  
N. D. Orlova ◽  
D. A. Egorova
Keyword(s):  
Multiplication Factor ◽  
Lonicera Caerulea ◽  
Genetic Characteristics ◽  
Clonal Micropropagation ◽  
Morphogenetic Potential

The clonal micropropagation technique of valuable Lonicera caerulea L. varieties (Indigo Gem, Moskovskaya 23, Diana, Jugana) has been improved. The effect of various types and concentrations of carbohydrates on microshoots regeneration of the genus Lonicera L. representatives was studied. It is shown that the concentration of carbohydrates has the greatest effect on microshoots height and the type of carbohydrate nutrition has the greatest effect on multiplication factor. The type of carbohydrate nutrition (42 %) had the greatest influence on multiplication factor. Peculiarities of varieties, genetic characteristics interaction, carbohydrate nutrition type and concentration had a lesser effect (20 %, 14 % and 11 % respectively). The variety Moskovskaya 23 was characterized by the highest microshoots number (49.6 mm). The highest multiplication factor in the studied varieties, regardless of the type of carbohydrate nutrition, was observed at concentration of 40 g/l (11.57).

scholarly journals Clonal micropropagation of bog cranberry (Vaccinium oxycoccos L.) in the Udmurt Republic

2021 ◽  
Vol 22 (1) ◽  
pp. 57-66
Author(s):  
E. N. Cheremnykh ◽  
T. G. Lekontseva ◽  
A. V. Khudyakova ◽  
A. V. Fedorov
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Root Formation ◽  
Sphagnum Moss ◽  
Multiplication Factor ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Udmurt Republic

The paper presents the results of 2018-2019 research on improving the technology of growing planting material of bog cranberry (Vaccinium oxycoccos L.) of Krasa Severa, Severyanka, Virussaare varieties on the basis of in vitro. Studied was the effect of the concentrations of growth regulators in the composition of the nutrient medium according to Anderson's recipe on the reproduction and subsequent rooting of micro cuttings, as well as the duration of cultivation and adaptation of micro plants depending on partial pruning of shoots. It has been established that at the stage of introduction into in vitro culture, sterilization of explants with 33% hydrogen peroxide in an exposure of 5-8 minutes with washing in 5 portions of sterile distillate gives 60-80 % of viable shoots. The optimum phase of plant development for the successful introduction of in vitro culture is the swelling of buds. Cultivation of micro cuttings was carried out in a light room at a temperature of 25±2 °С, a photoperiod of 16 hours. The duration of each subculturing was 30-60 days. For the stage of actual micropropagation on Anderson's nutrient medium, an increase in the dose of cytokinin 6-benzylaminopurine (6-BAP) from 0.2 to 0.5 mg/l and an increase in the duration of cultivation from 30 to 60 days contributed to a significant increase in the multiplication factor on average for the tested cranberry varieties.According to the efficiency of micropropagation, the varieties Virussaare and Krasa Severa were distinguished – 9.3-12.0 pcs/stalk, respectively. At the rooting stage, the use of a root-forming reagent of indolyl-3-acetic acid (IUK) in doses of 0.2, 0.5 and 1.0 mg/l in the composition of Anderson's nutrient medium did not affect the quality of root formation and the length of shoots of Virussaare micro-plants. No significant varietal differences in the root-forming ability of microcuttings were found. The tendency of better rooting of micro cuttings was observed in the Virussaare variety (90.3 %) compared to the Severyanka (85.7 %) and Krasa Severa (79.3 %) varieties. Micro plants of the Krasa Severa cultivar were characterized by the longest shoots, the total number of roots was less, but their length was longer in comparison with other cultivars. For the adaptation stage, a substrate from a mixture of lowland peat and sphagnum moss was used (1:1). The efficiency of adaptation of micro plants of cranberry varieties when cutting the tip of the shoots was 100 %. Pruning of micro plants shoots contributed to the formation of more side shoots and better development of the aboveground part of the plants.

scholarly journals Clonal micropropagation of essential oil rose cultivars and breeding samples at long-term subcultivation in vitro

2020 ◽  
Vol 224 ◽  
pp. 04010
Author(s):  
N. A. Yegorova ◽  
I V Stavtzeva
Keyword(s):  
Essential Oil ◽  
Morphometric Parameters ◽  
Rosa Damascena ◽  
The Third ◽  
Clonal Micropropagation ◽  
Morphogenetic Potential

The development of explants of 12 cultivars and breeding samples of essential oil rose (obtained with the participation of Rosa damascena Mill., R. gallica L., R. alba L.) during long-term micropropagation was investigated. At the second multiplication stage 5 subcultures were carried out. Increase of the studied morphometric parameters of explants to 3-4th subcultures was established. In some genotypes, the maximum multiplication index was in the third subculture (сultivars ‘Raduga’, ‘Zolushka’, ‘Lada’, ‘Krymskaya Krasnaya’, ‘Lany’, ‘Vesna’ and samples N37-24, M215), while in others (сultivar ‘Kazanlykskaya’ and samples N138, G2168, N37-2) – in the fourth. The largest increase of the multiplication index in 3-4th subcultures compared to the first (3.0-4.8 times) was found in the cultivars ‘Lada’, ‘Lany’, ‘Raduga’, ‘Krymskaya Krasnaya’ and sample N37-24. In the fifth subculture the multiplication index decreased. However, in most cultivars and breeding samples it was higher than in the first subculture. The best morphogenetic potential was noted for сultivar ‘Raduga’ and samples G2168, N37-2, in which the multiplication index reached 14.2, 14.4 and 11.8, respectively. The minimal ability to propagation in vitro was in samples M215 and N138 – their multiplication index did not exceed 1.1 - 4.0.

scholarly journals Influence of different concentrations of 6-benzylaminopurine and thidiazuron on the proliferative activity of apple varieties in in vitro culture

2021 ◽  
Vol 36 ◽  
pp. 03012
Author(s):  
L.V. Tashmatova ◽  
O.V. Matsneva ◽  
T.M. Khromova ◽  
V.V. Shakhov
Keyword(s):  
In Vitro Culture ◽  
Apical Dominance ◽  
Proliferative Activity ◽  
Essential Role ◽  
Cytokinin Activity ◽  
Multiplication Factor ◽  
Apple Varieties ◽  
Clonal Micropropagation ◽  
Study Results

An essential role in the process of clonal micropropagation is played by cytokinins, with the help of which apical dominance is removed and the formation of additional shoots and buds is activated. The article presents the study results of microshoots’ proliferative activity of apple varieties on media with 6-benzylaminopurine at a concentration of 1.0 mg/l, 2.0 mg/l and thidiazuron at a concentration of 0.1 mg/l, 0.2 mg/l and 0.3 mg/l. The objects of research were apple varieties of the VNIISPK selection - Bolotovskoe, Imrus, Veteran, Kandil orlovsky, Girlyanda, Priokskoe. The aim of the research was to study the effect of substances with cytokinin activity on the morphogenesis of apple varieties. As a result of the research, it was revealed that both cytokinins and varietal characteristics influence the multiplication factor. The highest multiplication factor was observed when using 6-BAP. Thidiazuron reduced the proliferation rate. When using 6-BAP, Bolotovskoe, Girlyanda and Priokskoe varieties had the highest multiplication factor among the varieties. When using TDZ - varieties Bolotovskoe and Kandil Orlovsky.

Some features of clonal micropropagation of decorative cultivars of Amelanchier Medik.

2020 ◽  
pp. 97-104
Author(s):  
E. N. Raeva-Bogoslovskaya ◽  
O. I. Molkanova
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Culture Conditions ◽  
Clonal Micropropagation ◽  
Morphogenetic Potential

In vitro culture conditions were optimized for representatives of the genus Amelanchier Medik. at the stages of micropropagation and rooting. A significant effect of the mineral and hormonal compositions of culture media on the morphogenetic potential of the cultivars of serviceberry has been established. The use at the stage of micropropagation of the MS culture medium with addition 1,0 mg / L 6-benzylaminopurine (6-BAP) promoted the active microshoot regeneration of the studied genotypes. For the induction of rhizogenesis the type of auxin as IB A at a concentration of 1,0 mg / L was used.

scholarly journals Improvement of clonal micropropagation technique of promising Lonicera caerulea L. cultivars

2021 ◽  
Vol 941 (1) ◽  
pp. 012030
Author(s):  
N D Orlova ◽  
O I Molkanova ◽  
O V Koroleva
Keyword(s):  
Nutrient Medium ◽  
In Vitro Propagation ◽  
Lonicera Caerulea ◽  
Clonal Micropropagation ◽  
Medium Components ◽  
Second Year ◽  
Propagation Technique ◽  
The Impact ◽  
Positive Effect

Abstract The work is focused on enhancement of in vitro propagation technique of valuable Lonicera caerulea L. cultivars. The impact of some nutrient medium components at main cultivation stages of the genus Lonicera L. representatives was studied. Some cultivars showed the positive effect of substituting sucrose for glucose in the medium at the multiplication stage (Diana (30 or 40 g L−1), Moskovskaya 23 (20 g L−1) and Yugana (40 g L−1)). When studying the influence of different auxin types on honeysuckle rhizogenesis, Solovey and Yugana preferred addition of 1.0 mg L−1 IBA into the medium. During the research of influence of different auxin concentrations on L. caerulea rooting the increased number of rooted microshoots of Goluboy Desert was demonstrated with the increasing of IAA concentration (from 0.5 to 3.0 mg L−1). Meanwhile, Diana and Yugana better rooted on the medium containing 1.0 mg L−1 IAA but Zolushka reached its highest rooting percentage on the medium supplemented with 0.5 mg L−1 IAA. The assessment of second-year plants undergone the whole cycle of clonal micropropagation was carried out 85% of these plants began to bear fruits.

Thickness of endometrium: predictor of the effectiveness of IVF/ICSI programs (literature review)

GYNECOLOGY ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 113-116
Author(s):  
L A Bagdasaryan ◽  
I E Korneyeva
Keyword(s):  
Assisted Reproductive Technologies ◽  
Endometrial Thickness ◽  
Molecular Genetic ◽  
Uterine Cavity ◽  
Reproductive Technologies ◽  
Genetic Characteristics ◽  
Vitro Fertilization ◽  
Need To Evaluate ◽  
The Relationship

The aim of the study is to systematically analyze the data available in the modern literature on the relationship between endometrial thickness and the frequency of pregnancy in the program of assisted reproductive technologies (ART). Materials and methods. The review includes data from foreign and domestic articles found in PubMed on this topic. Results. The article presents data on the relationship between the thickness of the endometrium and the frequency of pregnancy in ART programs. The greatest number of studies is devoted to the evaluation of the relationship between the thickness of the endometrium and the frequency of pregnancy on the day of the ovulation trigger. Data are presented on the existence of a correlation between the thickness of the endometrium measured on the day of the ovulation trigger and the frequency of clinical pregnancy, as well as data on the need to evaluate the structure of the endometrium and the state of subendometric blood flow. The importance of multilayered (three-layered) endometrium as a prognostic marker of success in in vitro fertilization/intracytoplasmic sperm injection programs in the ovum is emphasized. The conclusion. The thickness of the endometrium can not be used as an argument for canceling the cycle or abolishing embryo transfer to the uterine cavity. Further studies in this direction are needed with a study of the morphological and molecular genetic characteristics of the endometrium, which in the future will allow us to evaluate the relationship between the thickness of the endometrium and the probability of pregnancy.

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