Alkaline Phosphatase Activity of Human Cell Cultures.

1961 ◽  
Vol 107 (3) ◽  
pp. 532-534 ◽  
Author(s):  
H. M. Nitowsky ◽  
F. Herz
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Cell Cultures ◽  
Alkaline Phosphatase Activity ◽  
Human Cell ◽  
Human Cell Cultures

scholarly journals Alkaline inorganic pyrophosphatase activity of mammalian-cell alkaline phosphatase

1967 ◽  
Vol 105 (1) ◽  
pp. 155-161 ◽  
Author(s):  
Rody P. Cox ◽  
Paul Gilbert ◽  
Martin J. Griffin
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Mammalian Cell ◽  
Cell Cultures ◽  
Alkaline Phosphatase Activity ◽  
Inorganic Pyrophosphatase ◽  
Mammalian Cell Cultures ◽  
Single Protein ◽  
Pyrophosphatase Activity ◽  
Substrate Induction

Alkaline phosphatase prepared from mammalian cell cultures was found to have alkaline inorganic pyrophosphatase activity. Both of these activities appear to be associated with a single protein, as demonstrated by: (1) concomitant purification of alkaline phosphatase and alkaline inorganic pyrophosphatase; (2) proportional precipitation of alkaline phosphatase and inorganic pyrophosphatase activities by titrating constant amounts of an enzyme preparation with increasing concentration of antibody; (3) immune electrophoresis, which showed that precipitin bands that have alkaline phosphatase activity also have pyrophosphatase activity; (4) inhibition of pyrophosphatase activity by cysteine, an inhibitor of alkaline phosphatase activity; (5) similar subcellular localization of the two enzyme activities as demonstrated by histochemical methods; (6) hormonal and substrate induction of alkaline phosphatase activity in mammalian cell cultures, which produced a nearly parallel rise in inorganic pyrophosphatase activity.

scholarly journals ACID AND ALKALINE PHOSPHATASE IN WHITE CELLS DATA FOR THE LYMPHOCYTE AND THE POLYMORPHONUCLEAR LEUKOCYTE OF MAN AND THE RABBIT

Blood ◽  
1950 ◽  
Vol 5 (3) ◽  
pp. 267-277 ◽  
Author(s):  
W. F. HAIGHT ◽  
R. J. ROSSITER
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Statistical Analysis ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Human Cell ◽  
Human Lymphocyte ◽  
Polymorphonuclear Leukocyte ◽  
Cell Suspensions ◽  
Acid And Alkaline Phosphatase

Abstract 1. The acid and alkaline phosphatase activity has been determined on a series of suspensions of white cells obtained from both man and the rabbit by several different methods. 2. A statistical analysis of the results shows that for both species the alkaline phosphatase of white cell suspensions is confined chiefly to the polymorphonuclear leukocyte and the acid phosphatase is chiefly in the lymphocyte, although the polymonphonuclear leukocyte contains lesser concentrations of this enzyme also. 3. Although this qualitative distribution was the same for both species studied, quantitatively the rabbit differed from man. The activity of the alkaline phosphatase of the rabbit polymorphonuclear leukocyte was eight times that of the corresponding human cell, while the activity of the acid phosphatase of the human lymphocyte was more than twice that of rabbit lymphocyte.

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