scholarly journals Young and Old Sugar Apple (Annona squamosa Linn) Leaf Extracts As an Aedes aegypti Larva Insecticide

2019 ◽  
Author(s):  
Abdul Khair ◽  
Noraida Noraida
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Leaf Extracts ◽  
Control Group Design ◽  
Vector Borne Diseases ◽  
Apple Leaves ◽  
Significant Difference ◽  
Vector Borne

Vector-borne diseases are still a health problem in some tropical countries. One vector-borne disease is Dengue Hemorrhagic Fever (DHF). DHF never decreases and even tends to continue to increase, and many cause deaths in children, 90% of them attack children under 15 years. DHF cases in 2018 amounted to 65,602 cases, with 467 deaths (CFR = 0.71%). The behavior of holding water in various places such as tubs and tendons indirectly creates a breeding place for Aedes aegypti mosquitoes. The use of chemicals as insecticides can cause the death of non-target animals, environmental pollution, and the occurrence of vector resistance to insecticides. Therefore it is necessary to do other methods including the use of vegetable insecticides. Sugar apple leaves can be used to kill Aedes aegypti larvae. Sugar apple leaves will obtain throughout the year. The purpose of this study is to know the ability of young and old sugar apple leaves to kill Aedes aegypti larvae. This type of research is a posttest only control group design. The study sample was part of the Aedes aegypti larvae on the final instar III. The experiment to kill Aedes aegypti larvae use two types of sugar apple leaf extract, namely young and old sugar apple leaves with nine treatments four replications. Each treatment consisted of 25 larvae. The research results show There was no significant difference in the number of dead larvae using either extracts from young sugar apple leaves or old sugar apple leaves. LC90 of sugar apple leaf extract was between 0.05632 to 0.08324% and the effective residual age at LC90 (0.06568%) with the death of Aedes aegypti larvae was 92% over 24 hours (1 day).

scholarly journals Biolarvasides of Cananga odorata Flower and Zyzygium polyanthum Leaves Ethanol Extract on Aedes aegypti Larva

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Nurlailah Nurlailah ◽  
Anny Thuraidah
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Public Health Problem ◽  
Ethanol Extract ◽  
Hemorrhagic Fever ◽  
Wilcoxon Test ◽  
Control Group ◽  
Control Group Design ◽  
Flower Extract ◽  
Negative Impacts

Dengue hemorrhagic fever (DHF) is one of the diseases caused by Aedes Aegypti mosquitoes. DHF is still a public health problem, including in Indonesia, due to the high mortality rate caused by this mosquito. The risk caused by the bite of the Aedes sp mosquito is quite dangerous, including acute fever, bleeding, shock, and even death, so that efforts are needed to control it. One of the natural ingredients that can use to eradicate Aedes Aegypti mosquitoes without causing negative impacts on the environment is the Cananga flower and Zyzigium Leaves. This study purposes of determining the optimum concentration of Cananga Flowers and Zyzigium Leaves Ethanol extract of which was having biolarvaside effect to Aedes Aegypti larvae. The method used a simple experiment with Posttest Only With Control Group Design. The data result of the research analyzed with Logit and Wilcoxon test. Achievement of those tests was LC50 Zyzigium leaf extract on contact for 24 hours was 0.609-0.935, and LC99 was between 1.064-1.973 while Cananga flower extract obtained LC50 of 0.228-0.261 and the LC99 was 0.446 to 0.558. Wilcoxon test for compared bioalarvaside effect results between Cananga Flower and Zyzigium Leaf received a significance value less than α (0.001<0.05), so it can ascertain that the Cananga Flower extract was stronger biolarvaside than Zyzigium Leaf extract.

scholarly journals Lethal Efficacy of Banana Leaves Extract (Musa paradisiaca L.) Against Aedes aegypti Larvae

2021 ◽  
Vol 4 (1) ◽  
pp. 7-12
Author(s):  
Wihdatul Karima ◽  
Syahrul Ardiansyah
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Test Group ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Mosquito Larvae ◽  
Control Group Design ◽  
Musa Paradisiaca ◽  
Banana Leaves ◽  
Banana Leaf

Indonesia has a potential to be exposed to the threat of dengue hemorrhagic fever with the point vector, the Aedes aegypti mosquito, so development need to be stopped. The use of synthetic larvicides in eradicating mosquito larvae can cause environmental pollution. To reduce it, an alternative is needed using plant larvacides with banana leaf. Banana leaves are obtained from Candi Sidoarjo. Ethanol extract of banana leaf contains tannin compounds, alkaloids, terpenoids, saponins, and flavonoids which can be used as larvicides. This research was conducted to determine the toxic effects of banana leaf extract (Musa paradisiaca L.) on the larvae of Aedes aegypti mosquito mortality. This research was conducted using the post test only the control group design with 6 treatment groups including control (aquades) and banana leaf  extract concentrations of 1000 ppm, 2000 ppm, 3000 ppm, 4000 ppm, 5000 ppm. This study used third instar larvae, each test group countaining 20 larvae with 4 repetitions. That obtained were analyzed using data and probit tests. The results of this study that banana leaf extract has a toxic effect on Aedes aegypti mosquito larvae with LC50 at a concentration of 4638 ppm.

scholarly journals Larvacide effectiveness of Papaya leaf extract (Carica papaya) on the mortality of larvae vector of Dengue hemorrhagic fever caused by Aedes aegypti

2021 ◽  
Vol 913 (1) ◽  
pp. 012110
Author(s):  
N F Dhenge ◽  
P Pakan ◽  
K Lidia
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design

Abstract Dengue Hemorrhagic Fever (DHF) is an infection caused by the dengue virus, which is still a public health problem in Indonesia. Chemical insecticides as larvicides used to control Aedes aegypti have created resistant populations. Thus, higher doses are needed, which, of course, have toxic effects on animals, humans, and the environment.To analyze the effectiveness of papaya leaf extract (Carica papaya) against the mortality of the third instar Aedesaegypti mosquito larvae. This study is a true experimental research design with a posttest-only control group design. In this study, the groups in this study were the larvae of the Aedes aegypti mosquito instar III/IV, papaya leaf ethanol extract, abate as a positive control group, and aquadest as a negative control.The results of the mean number of larvae deaths in each treatment group was zero (0) larvae in negative control group, positive control group (25 larvae), 5% concentration (9.5 larvae), 10% concentration (11.75 larvae), 15% concentration (12.75 larvae), a concentration of 20% (14.75 larvae), and a concentration of 25% (19.5 larvae). The results of Kruskal Wallis analysis showed that papaya leaf extract was effective as a larvicide for Aedesaegypti (p=0.001). In the probit analysis, it was found that the LC50 of the extract against Aedes aegypti was 23%, while the LC99 was 55%.Papaya leaf extract is effective as a vegetable larvicide for 3rd/IV instar Aedesaegypti larvae

scholarly journals Efektivitas Ekstrak Biji Bintaro (Cerbera manghas) sebagai Larvasida Hayati pada Larva Aedes aegypti Instar III

2018 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Khoiriyanti Wulandari ◽  
Mei Ahyanti
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Contact Time ◽  
Statistical Tests ◽  
Hemorrhagic Fever ◽  
Seed Extract ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Cerbera Manghas

<p>Dengue Hemorrhagic Fever (DHF) or Dengue Hemorrhagic Fever (DHF) is a disease caused by a virus which is very dangerous because it can cause the patient to die within a few days. Bandar Lampung city itself is a dengue endemic area. According to data from the Lampung Provincial Health Office, in 2015, from 15 there were 2,996 deaths in 31 people. The study aimed to knowing the effectiveness of bintaro seed extract (Cerbera manghas) as larvacide in III instars Aedes aegypti larvacides experimental research with post test only control group design. The sampling method was purposive sampling. Independent variables were bintaro seed extract (dose and time), the dependent variable was Aedes aegypti larvAedes The results showed that from 5 types of doses with 5 repetitions, the death result of Aedes aegypti larvae with a dose of 0% had no mortality, a dose of 0.1% with an average of 12.55, a dose of 0.5% with an average of 15.15 , 1% dose with an average of 18.20, a dose of 1.5% with an average of 22.15, and a dose of 2% with an average of 25. At the time of contact, the death result of the larvae was found with an average 6-hour contact time. an average of 8.70, 12 hours with an average of 14.13, 24 hours with an average of 18.37 and 48 hours with an average of 20.83. According to the results of statistical tests, the most effective effect of bintaro seed extract dosage is at a dose of 1.5% and contact time of 6 hours.<strong></strong></p>

scholarly journals THE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC® 6514™ IN VITRO

2018 ◽  
Vol 11 (12) ◽  
pp. 411
Author(s):  
Ameta Primasari ◽  
Minasari Nasution ◽  
Nurul Hidayati Arbi ◽  
Dini Permata Sari ◽  
Mohammad Basyuni
Keyword(s):  
Leaf Extract ◽  
Brain Heart Infusion ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Negative Control ◽  
Plate Count ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Mueller Hinton Agar

Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC® 6514™ growth.Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCC® 6514 ™ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskal–Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.Results: Concentration of MIC from soursop leaf extract on Aa ATCC® 6514™ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCC® 6514™ from each treatment group.Conclusion: Soursop leaf extract has antibacterial effectivity against Aa ATCC® 6514 ™.

scholarly journals EFFECTIVENESS OF LOTION EXTRACT OF VARIOUS TYPE OF PLANTS AS Aedes aegypti MOSQUITO REPPELENT

2020 ◽  
Vol 39 (4) ◽  
pp. 203-209
Author(s):  
Agus - Subagiyo
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Statistical Tests ◽  
Hemorrhagic Fever ◽  
Negative Control ◽  
Toxic Substances ◽  
Research Needs ◽  
Protective Capacity ◽  
Leaf Extracts ◽  
The Difference

Effort to control DHF (Dengue Hemorrhagic Fever) vectors without toxic substances by using natural and enviromentally friendly materials was natural repellent made using plants that were widely available in the community.Many types of plants contain  various secondary metabolite compound substances that have the potential to be used as natural insecticides, especially as repellent of the Aedes Aegypti mosquito. The research needs  to be done to determine the effectiveness of basil leaves, citronella leaves, clove leaves and betel leaves as a repellent against Aedes Aegypti mosquitoes. This research method was an experiment in the laboratory. The materials used for this research were basil leaves, citronella leaves, clove leaves, and betel leaves. The extracted leaves were then made lotions with certain formulas, tested as a natural repellent against Aedes Aegypti mosquitoes. The concentrations used were 20%, 40% and 80% as well as negative control (0%) and positive control (brand x lotions). The number of mosquitoes used in the study were 25 x 4 treatments = 100 mosquitoes. The replication or repetition was done 10 times. To find out the difference in the number of Aedes Aegypti mosquitoes that perch on the hands of probandus which has been smeared with basil leaf, citronella leaf, clove leaf and betel leaf extracts uses Anova Statistical tests and kruskal wallis. To see the difference between concentrations was tested with LSD or Mann-Whitney test. The calculation result of the number of Aedes Aegypti mosquitoes that perch before and after applying the lotion, the lowest was the lotion of citronella leaf extract 0% (11); 20% (2); 40% (1); 80% (1); The highest protection capacity of extract lotion is citronella leaves with concetrations of 20% (81,1%); 40% (95%), 80%(98,2%); There were no significant differences between the lotion concentrations of 20% and 80%, while the concentration of 40% is significantly different. Then the LSD follow-up test finds that the protective capacity of lotion of clove leaf extract is negative and citronella leaf extract is positive. The most effective protection capacity against Aedes Aegypti mosquitoes was lotion of citronella leaf extract with concentration of 40% (95%) and a concentration of 80% (98,2%), proven by the value of protection capacity that is stable even though it has been used for up to 6 hours and can exceed the protection capacity of lotion x (90,3%). It is reccomended that further research needs to be developed to optimize the lotion of citronella leaf extract 40% and 80% with orgonolaptic test of color, aroma, stickness and comfort of its use.

scholarly journals The Effect of Sugar Apple (Annona squamosa L.) Seeds Solution as an Insecticide Against Mortality of Aedes aegypti Mosquito with Liquid Electric Method

2018 ◽  
Vol 9 (2) ◽  
pp. 200
Author(s):  
Amalinda Mega Novasari ◽  
Retno Sasongkowati
Keyword(s):  
Aedes Aegypti ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Cross Sectional Study ◽  
Primary Data ◽  
Control Group ◽  
Annona Squamosa ◽  
Control Group Design ◽  
Cross Sectional ◽  
Apple Seeds

Dengue hemorrhagic fever is a public health problem in Indonesia that the number of DHF cases has been increased. Morbidity number of DHF per 100,000 population at 2011 was 26.67 became 34.3 in 2012. The control of DHF has been attempted, but a lot of synthetic insecticide used has been negatively affected to environment. So, it need the insecticide alternative. The aim of study was to identify the effect of solvent sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method. Type of this research was experimental with cross sectional study design as well as the post test only control group design. The study was conducted from February to July 2013 in the Laboratory of Entomology of the Provincial Health Office of East Java. The population of this research was the Aedes aegypti with 560 sample of mosquitoes were divided into five treatments and four repetitions for each treatment. The collection of quantitative data drawn from primary data through observation by calculating the death of Aedes aegypti of each the sugar apple seeds concentration of 10%, 20%, 30%, 40% and 50% for 24 hours. The results showed that the concentration of 50% solvent of sugar apple seeds provide the greatest effect in causing the death of Aedes aegypti mosquitoes as many as 36 died after exposure. It can be concluded that there are effected by giving solvent of sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method.

scholarly journals ANTIBACTERIAL EFFECTIVITY OF KASTURI LEAF EXTRACT (Mangifera casturi) AGAINST THE GROWTH OF Streptococcus sanguinis BACTERIA

2020 ◽  
Vol 5 (1) ◽  
pp. 33
Author(s):  
Dita Puspita Sari ◽  
Didit Aspriyanto ◽  
Irham Taufiqurrahman
Keyword(s):  
Leaf Extract ◽  
Periodontal Diseases ◽  
Tooth Surface ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Streptococcus Sanguinis ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Background: Caries is a disease that occurs because of the fermentation carbohydrates process by microorganisms in the oral cavity. One of the bacteria that causes caries is Streptococcus sanguinis. These bacteria will colonize on the tooth surface, then form dental plaques and contribute to the causes of caries and other periodontal diseases. Kasturi leaf extract (Mangifera casturi) has various compounds such as tannins, terpenoids, alkaloids, and flavonoids that have antimicrobial substances. Purpose: The aim of this study was to determine antibacterial effectivity of kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis bacteria. Method: This research was an experimental method laboratory (true experimental), with a randomized pre test and post test with control group design using 5 treatments: kasturi leaf extract (concentration: 20 mg/ml, 25 mg/ml, and 30 mg/ml); and two groups of control: positive control and negative control. Each treatment was repeated 5 times. Antibacterial activity testing used a liquid dilution method. Measurement of minimum inhibitory concentration (MIC) used a Uv-Vis Spectrophotometer and measurement of the minimum bactericidal concentration (MBC) used a colony counter. The MIC data were analyzed using One Way Anova and continued with the Dunnet Post Hoc test. MBC data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney Post Hoc test. Result: One-Way Anova test showed that MIC had a significant difference, and the Kruskal-Wallis test showed that MBC also had significant differences. MIC was obtained at the concentration of 20 mg/ml and MBC was obtained at the concentration of 30 mg / ml. Conclusion: There is antibacterial effectiveness in kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis.

scholarly journals Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users

2018 ◽  
Vol 1 (1) ◽  
pp. 35 ◽  
Author(s):  
Tiara Meilena ◽  
Januar Cidie Fabiansyah ◽  
Eha Djulaeha ◽  
Hanoem Eka Hidayati
Keyword(s):  
Mtt Assay ◽  
Leaf Extract ◽  
Fibroblast Cell ◽  
Colocasia Esculenta ◽  
Laboratory Research ◽  
Control Group ◽  
In Vitro Tests ◽  
Fibroblast Cells ◽  
Leaf Extracts ◽  
Control Group Design

Background: One of requirements for a material to be used in dentistry is that the material must be non toxic and non irritant, as well as have biocompatibility properties, not triggering a detrimental effect on the biological environment, both locally and systemically. The first-line tests that must be conducted is in vitro tests, one of which is MTT Assay. Samples used in this research were fibroblast cells (cultured BHK-21 cells) since fibroblasts are the most important and largest components in pulp, periodontal ligament, and gingiva. Purpose: This research aimed to determine toxicity effect of taro leaf extracts (Colocasia esculenta L. Schoot) on BHK-21 fibroblast cells. Methods: This research is an experimental laboratory research using Post Test Only Control Group design.  The research treatment are the administration of Taro leaf extract (Colocasia esculenta L. Schoot)  with 100%, 80%, 60%, and 40% concentration on BHK-21 fibroblast cell. Results: The fibroblast cell life percentage in 100%, 80%, 60%, and 40% concentration respectively are 106,3%, 50,9%, 36,2%, and 30,1%. The toxicity results are obtained using MTT assay technique after 24 hours. The optical density absorbency values are read by ELISA reader and represent life cell viability. Conclusion: Taro leaf extract (Colocasia esculenta L. Schoot) at a concentration above 80% is considered as non-toxic material to BHK-21 fibroblast cells

scholarly journals THE EFFECTIVENESS TEST OF BETEL LEAF EXTRACT TOWARD THE DEVELOPMENT OF AEDES AEGYPTI SP LARVAE

Biospecies ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 1-7
Author(s):  
Firza Khairullah Sembiring ◽  
Riyani Susan BT Hasan
Keyword(s):  
Aedes Aegypti ◽  
Experimental Method ◽  
Leaf Extract ◽  
Sampling Method ◽  
Total Mortality ◽  
Hemorrhagic Fever ◽  
Betel Leaf ◽  
Significant Difference ◽  
Post Test ◽  
Confidence Index

This research was conducted to determine the effectiveness of betel leaf extract conducted at a concentration of 2.5%, 5.0%, 7.5%, 10% as larvicide toward the development of Aedes Aegypti Sp. Aedes Aegypti Sp larvae are dangerous vectors in Dengue Hemorrhagic Fever (DHF) in humans. The method in this research was carried out on an experimental method by using a post-test only design and sampling with a purposive sampling method. The effectiveness of betel leaf extract larvicide on the development of Aedes Aegypti Sp larvae was carried out by mixing betel leaf extract with several concentrations in one container Aedes Aegypti Sp larvae inside. The results obtained in this research were carried out by the Kruskal-Wallis test, the test found that there was a significant difference because it was given a 95% confidence index. The larvae treated with betel leaf extract with a concentration of 10% had the highest effectiveness in stopping the development of Aedes Aegypti Sp larvae, which proved at the mortality at a concentration of 10%, found total mortality of 20 larvae in 8 hours of treatment. Betel leaf extract with a concentration of 10% had the highest effectiveness of discontinuing the development of Aedes Aegypti Sp larvae.

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