scholarly journals Carbon C 13 Sodium Octanoate

2020 ◽  
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2016 ◽  
Vol 27 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Mihaela Carmen Eremia ◽  
Irina Lupescu ◽  
Mariana Vladu ◽  
Maria Petrescu ◽  
Gabriela Savoiu ◽  
...  

Abstract Polyhydroxyalcanoates (PHAs) are specifically produced by a wide variety of bacteria, as an intracellular energy reserve in the form of homo- and copolymers of [R]-β-hydroxyalkanoic acids, depending on the C source used for microorganism growth, when the cells are grown under stressing conditions. In this paper we present microbiological accumulation of poly-3-hydroxyoctanoate (PHO) by using a consortium of bacterial strains, Pseudomonas putida and Bacillus subtilis, in a rate of 3:1, grown on a fermentation medium based on sodium octanoate as the sole carbon source. The experiments performed in the above mentioned conditions led to the following results: from 18.70 g sodium octanoate (7.72 g/L in the fermentation medium) used up during the bioprocess, 3.93-3.96 g/L dry bacterial biomass and 1.834 - 1.884 g/L PHA, containing 85.83 - 86.8% PHO, were obtained.


1975 ◽  
Vol 53 (1) ◽  
pp. 90-101 ◽  
Author(s):  
Rauno Friman ◽  
Kerstin Pettersson ◽  
Per Stenius
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1982 ◽  
Vol 93 (1) ◽  
pp. 91-94 ◽  
Author(s):  
John B. Hayter ◽  
Thomas Zemb
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2011 ◽  
Vol 30 (4) ◽  
pp. 328-333 ◽  
Author(s):  
Suleyman Aydin ◽  
Sebnem Erenler ◽  
Yalcin Kendir

Effects of Sodium Octanoate, Acylated Ghrelin, and Desacylated Ghrelin on the Growth of Genetically EngineeredEscherichia ColiAcylated ghrelin is a 28-amino acid peptide hormone bearing a fatty acid group based on octanoic acid (caprylic acid) at the serine which is located at position 3 and at the N-terminus. If this fatty acid is cleaved from acylated ghrelin, the remaining peptide is referred to as desacylated ghrelin. Free fatty acids (FFAs) can kill or inhibit the growth of bacteria. The purpose of this study was to test this ability using acylated ghrelin, desacylated ghrelin, and sodium octanoate (caprylic acid) as carbon sources for the genetically engineeredEscherichia colistrains MK79 and MK57. For this experimental work, minimal medium was modified by replacing glucose with equal concentrations of acylated ghrelin, desacylated ghrelin, or sodium octanoate. Bacterial optical density, viability, alpha-amylase production, plasmid stability and pH of the growth medium were measured during these experiments. The media that allowed most growth, based on viable cell counts and the OD600of MK79, was minimal medium, followed by the medium containing desacylated ghrelin or acylated ghrelin, and finally the medium containing sodium octanoate. The same order was observed for MK57. Neither of the strains lost plasmids during the entire course of each experiment. There was also little change in the pH of any of the media used for both strains. These results suggest that sodium octanoate, acylated ghrelin, and desacylated ghrelin, when compared with minimal medium, inhibitEscherichia coligrowth. Proliferation was lowest when sodium octanoate was used as the carbon source, followed by acylated ghrelin and desacylated ghrelin. Therefore, the acylated ghrelin found previously in human saliva might help to inhibit pathogenic microorganisms, and acylated ghrelin levels below a critical concentration in saliva could result in an increased risk of oral infection.


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