scholarly journals Preparation of bio - adhesive hydrogel based on chitosan for wound sealant

2015 ◽  
Vol 18 (2) ◽  
pp. 88-95
Author(s):  
Diem Thi My Pham ◽  
Hoa Thi Hoang ◽  
Vu Dinh Dang ◽  
Quyen Ngoc Tran
Keyword(s):  
Hydrogen Peroxide ◽  
Wound Healing ◽  
Horseradish Peroxidase ◽  
Biomedical Applications ◽  
Fibroblast Cell ◽  
Tissue Adhesive ◽  
Chitosan Hydrogel ◽  
Tissue Adhesion ◽  
Adhesive Forces

In this study, we introduce a new kind of hydrogel based on oxidized chitosan for tissue adhesion. The hydrogel formed rapidly in a few seconds in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The in vitro cytocompatible experiment with fibroblast cell using kit live/dead assay showed that the hydrogel was highly biocompatible. Evaluation of tissue adhesion performed on pork skin the maximum tissue adhesive forces are 88 kPa for chitosan hydrogel and 105 kPa for oxidized chitosan hydrogel.. These results suggest that chitosan hydrogel possessed the wound healing ability and promises a tissue adhesive devices for biomedical applications.

scholarly journals β-Cyclodextrin/Triclosan Complex-Grafted Methacrylated Glycol Chitosan Hydorgel by Photocrosslinking via Visible Light Irradiation for a Tissue Bio-Adhesive

2021 ◽  
Vol 22 (2) ◽  
pp. 700
Author(s):  
Young Jae Moon ◽  
Sun-Jung Yoon ◽  
Jeung-Hyun Koo ◽  
Yihyun Yoon ◽  
Hye Jun Byun ◽  
...  
Keyword(s):  
Wound Healing ◽  
Bacterial Infection ◽  
Visible Light ◽  
Visible Light Irradiation ◽  
Light Irradiation ◽  
Tissue Adhesive ◽  
Proton Nuclear Magnetic Resonance ◽  
Scanning Calorimetry ◽  
Glycol Chitosan

Accelerating wound healing with minimized bacterial infection has become a topic of interest in the development of the new generation of tissue bio-adhesives. In this study, we fabricated a hydrogel system (MGC-g-CD-ic-TCS) consisting of triclosan (TCS)-complexed beta-cyclodextrin (β-CD)-conjugated methacrylated glycol chitosan (MGC) as an antibacterial tissue adhesive. Proton nuclear magnetic resonance (1H NMR) and differential scanning calorimetry (DSC) results showed the inclusion complex formation between MGC-g-CD and TCS. The increase of storage modulus (G’) of MGC-g-CD-ic-TCS after visible light irradiation for 200 s indicated its hydrogelation. The swollen hydrogel in aqueous solution resulted in two release behaviors of an initial burst and sustained release. Importantly, in vitro and in vivo results indicated that MGC-g-CD-ic-TCS inhibited bacterial infection and improved wound healing, suggesting its high potential application as an antibacterial tissue bio-adhesive.

scholarly journals Application ofAntrodia camphorataPromotes Rat’s Wound HealingIn Vivoand Facilitates Fibroblast Cell ProliferationIn Vitro

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Zahra A. Amin ◽  
Hapipah M. Ali ◽  
Mohammed A. Alshawsh ◽  
Pouya H. Darvish ◽  
Mahmood A. Abdulla
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Granulation Tissue ◽  
Inflammatory Cell ◽  
Biological Activities ◽  
Fibroblast Cell ◽  
Parasitic Fungus ◽  
Antrodia Camphorata

Antrodia camphoratais a parasitic fungus from Taiwan, it has been documented to possess a variety of pharmacological and biological activities. The present study was undertaken to evaluate the potential ofAntrodia camphorataethanol extract to accelerate the rate of wound healing closure and histology of wound area in experimental rats. The safety ofAntrodia camphoratawas determinedin vivoby the acute toxicity test andin vitroby fibroblast cell proliferation assay. The scratch assay was used to evaluate thein vitrowound healing in fibroblast cells and the excision model of wound healing was testedin vivousing four groups of adultSprague Dawleyrats. Our results showed that wound treated withAntrodia camphorataextract and intrasite gel significantly accelerates the rate of wound healing closure than those treated with the vehicle. Wounds dressed withAntrodia camphorataextract showed remarkably less scar width at wound closure and granulation tissue contained less inflammatory cell and more fibroblast compared to wounds treated with the vehicle. Masson’s trichrom stain showed granulation tissue containing more collagen and less inflammatory cell inAntrodia camphoratatreated wounds. In conclusion,Antrodia camphorataextract significantly enhanced the rate of the wound enclosure in rats and promotes thein vitrohealing through fibroblast cell proliferation.

scholarly journals Development of Antimicrobial Conjugates and Evaluating its Antibacterial potential and Wound Healing ability

2021 ◽  
Vol 23 (09) ◽  
pp. 540-555
Author(s):  
Deepak Tom Jose ◽  
◽  
Sivagurunathan, P ◽  
Aswini, B ◽  
Dinesh, MD ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
L929 Fibroblast ◽  
Microscopic Studies ◽  
Engineered Materials ◽  
Cell Migration And Proliferation ◽  
Antibacterial Potential

Antimicrobial peptides from Streptomyces sp. and marine fish (Carangoides malabaricus) were extracted and developed as conjugates in the present study. The objective was framed to analyze the ability of conjugate to retard the growth of test bacteria causing diabetic foot ulcers. Fibroblast cell adhesion on AMP conjugates coated mesh samples were recorded using microscopic studies with an aim of developing a novel tissue engineered wound dressing material. Thus developed tissue engineered materials were evaluated for its antibacterial potential against wound pathogens; and to assay the wound healing ability using a standard in vitro wound scratch method. Tissue engineered materials were developed using L929 fibroblast cells. L929 fibroblast cells attachment and its stage wise development on wound dressing mesh materials were microscopically observed. In vitro wound healing assay revealed that the developed conjugates (containing AMPs) exhibited cell migration and proliferation after 12th hour of incubation indicating the wound healing abilities. The results showed that the developed tissue engineered wound dressing material has commercial interest in near future.

scholarly journals Wound Healing Activity of α-Pinene and α-Phellandrene

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2488
Author(s):  
Judith Salas-Oropeza ◽  
Manuel Jimenez-Estrada ◽  
Armando Perez-Torres ◽  
Andres Eliu Castell-Rodriguez ◽  
Rodolfo Becerril-Millan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Folk Medicine ◽  
Fibroblast Cell ◽  
In Vitro Tests ◽  
Low Concentrations ◽  
In Vivo Tests ◽  
Wound Healing Activity

Bursera morelensis is used in Mexican folk medicine to treat wounds on the skin. Recently, it was shown that the essential oil (EO) of B. morelensis has wound healing activity, accelerating cutaneous wound closure and generating scars with good tensile strength. α-pinene (PIN) and α-phellandrene (FEL) are terpenes that have been found in this EO, and it has been shown in different studies that both have anti-inflammatory activity. The aim of this study was to determine the wound healing activity of these two terpenes. The results of in vitro tests demonstrate that PIN and FEL are not cytotoxic at low concentrations and that they do not stimulate fibroblast cell proliferation. In vivo tests showed that the terpenes produce stress-resistant scars and accelerate wound contraction, due to collagen deposition from the early stages, in wounds treated with both terpenes. Therefore, we conclude that both α-pinene and α-phellandrene promote the healing process; this confirms the healing activity of the EO of B. morelensis, since having these terpenes as part of its chemical composition explains part of its demonstrated activity.

Zebrafish fin-derived fibroblast cell line: A model for in vitro wound healing

2019 ◽  
Vol 42 (4) ◽  
pp. 573-584 ◽  
Author(s):  
Nathiga Nambi Kalaiselvi Sivalingam ◽  
Abdul Majeed Seepoo ◽  
Taju Gani ◽  
Sivakumar Selvam ◽  
Sahul Hameed Azeez Sait
Keyword(s):  
Wound Healing ◽  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line
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