scholarly journals Chemical constituents and theira-glucosidase inhibitory activity of the stem of Salacia chinensis L.

2021 ◽  
Vol 5 (3) ◽  
pp. first
Author(s):  
Huu Tho Le ◽  
Truc Thanh Thi Duong ◽  
Phu Hoang Dang ◽  
Truong Nhat Van Do ◽  
Hai Xuan Nguyen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Back Pain ◽  
Inhibitory Activity ◽  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Positive Control ◽  
Ic50 Values ◽  
Treatment Of Diabetes ◽  
Chcl3 Fraction

Salacia chinensis L., known as “Chop mao” in Vietnam, is a climbing shrub that belongs to the Celastraceae family. The stem of S. chinensis L. is used as a traditional medicine for the treatment of diabetes, rheumatoid arthritis, back pain, … The dried powdered stem of S. chinensis L. was collected in Phu Yen province and was extracted with methanol to yield methanol extract. The methanol extract was suspended in H2O and partitioned successively with n-hexane, CHCl3, EtOAc to obtain n-hexane, CHCl3, EtOAc, and H2O fractions, respectively. The CHCl3 fraction was subjected to a series of chromatographic separation to afford four purified compounds including 3-oxolup-20(29)-en-30-al (1), betulin-3-caffeate (2), 2-(4-hydroxy-3,5-dimethoxylphenyl)ethanol (3), and acetosyringone (4). Their structures were elucidated on the basis of the spectroscopic analysis and comparison with literature data. The isolated compounds were tested for their α-glucosidase inhibitory activity. The result indicated that all compounds (1-4) possessed significant α-glucosidase at the testing concentration of 100 µM with the percent inhibition values of 9.5 ± 1.3, 70.89 ± 0.25, 44.2 ± 1.6, and 6.7 ± 1.7 %, respectively. In addition, betulin-3-caffeate (2) and 2-(4-hydroxy-3,5-dimethoxylphenyl)ethanol (3) show more potent α-glucosidase inhibitory activity, with IC50 values of 69.7 and 152.0 µM, respectively, than that of positive control acarbose (IC50 = 214.5 µM).

scholarly journals Chemical constituents and their alpha-glucosidase inhibitory activity from the stems of taxotrophis ilicifolius

2021 ◽  
Vol 5 (3) ◽  
pp. 1334-1340
Author(s):  
Truong Nhat Van Do ◽  
Hai Xuan Nguyen ◽  
Tho Huu Le ◽  
Mai Thanh Thi Nguyen
Keyword(s):  
Inhibitory Activity ◽  
Chemical Constituents ◽  
Syringic Acid ◽  
Positive Control ◽  
Traditional Use ◽  
Chromatography Method ◽  
Chemical Structures ◽  
Phenolic Constituents ◽  
Ic50 Values ◽  
Treatment Of Diabetes

Taxotrophis ilicifolius, called "Gai quít" in Vietnam, belonged to the family of Moraceae. The stems of T. ilicifolius are used in traditional medicine to cure pimples, anti-inflammatory, antibacterial... By column chromatography method together with thin layer chromatography on the ethyl acetate extract of its stems, six compounds were isolated including wogonin (1), 4-hydroxybenzoic acid (2), vanillic acid (3), vanillin (4), syringic acid (5), and syringaldehyde (6). Their chemical structures were elucidated by extensive NMR spectroscopic analysis and comparison with the literature data. The isolated compounds were tested fora-glucosidase inhibitory activity. All compounds showed more potent inhibitory activity with IC50 values 42–142 mM, than that of a positive control acarbose (IC50, 214.5 mM). Among all isolates, compounds 2, 4 and 6 exhibited significant a-glucosidase inhibitory activity with the IC50 values of 49.7, 42.6 and 55.9 mM, respectively. From the present investigation, all these compounds were isolated for the first time as well as their a-glucosidase inhibitoryactivity of T. ilicifolius. These results suggested that the traditional use of T. ilicifolius for the treatment of diabetes disease in Vietnam may be due to thea-glucosidase inhibitory activity of its phenolic constituents.

Inhibition of Protein Tyrosine Phosphatase 1B by Lutein Derivatives isolated from Mexican Oregano (Lippia graveolens)

2018 ◽  
Vol 17 (3) ◽  
pp. 134-139
Author(s):  
R.M. Perez-Gutierrez
Keyword(s):  
Protein Tyrosine Phosphatase ◽  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
Methanol Extract ◽  
Tyrosine Phosphatase ◽  
Positive Control ◽  
Protein Tyrosine Phosphatase 1B ◽  
Protein Tyrosine ◽  
Ic50 Value ◽  
Ic50 Values

Methanol extract from Lippia graveolens (Mexican oregano) was studied in order to identify inhibitory bioactives for protein tyrosine phosphatase 1B (PTP1B). Known flavone as lutein (1), and another flavone glycoside such as lutein-7-o-glucoside (2), 6-hydroxy-lutein-7-ohexoside (3) and lutein-7-o-ramnoide (4) were isolated from methanol extract of aerial parts of the Lippia graveolens. All isolates were identified based on extensive spectroscopic data analysis, including UV, IR, NMR, MS and compared with spectroscopic data previously reported. These flavones were evaluated for PTP1B inhibitory activity. Among them, compounds 1 and 3 displayed potential inhibitory activity against PTP1B with IC50 values of 7.01 ± 1.25 μg/ml and 18.4 μg/ml, respectively. In addition, compound 2 and 4 showed moderate inhibitory activity with an IC50 value of 23.8 ± 6.21 and 67.8 ± 5.80 μg/ml respectively. Among the four compounds, luteolin was found to be the most potent PTP1B inhibitor compared to the positive control ursolic acid, with an IC50 value of 8.12 ± 1.06 μg/ml. These results indicate that flavonoids constituents contained in Lippia graveolens can be considered as a natural source for the treatment of type 2 diabetes.

scholarly journals Chemical constituents and their alpha-glucosidase inhibitory activity of Solanum procumbens

2021 ◽  
Vol 5 (3) ◽  
pp. 1326-1333
Author(s):  
Nhan Trung Nguyen ◽  
Truong Nhat Van Do ◽  
Tho Huu Le ◽  
Phu Hoang Dang ◽  
Hai Xuan Nguyen ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Positive Control ◽  
Scientific Data ◽  
Chemical Compositions ◽  
Chromatography Method ◽  
Perennial Shrub ◽  
Ic50 Values ◽  
Folk Remedies

Solanum procumbens called ``Cà gai leo'' in Vietnam, belonged to the family of Solanaceae, which is a prickly diffuse, bright green perennial shrub, woody at the base. This plant has been sought for plenty of Vietnamese folk remedies for diseases such as rheumatism, back pain, detoxification, cough, pain relief, hemostasis, hepatitis, and cirrhosis. Chemical reports revealed the presence of lots of secondary metabolites such as steroids, triterpenoids, alkaloids, and phenolic compounds that have good biological activities such as antibacterial, antidiabetic, antioxidant, and antimicrobial... By column chromatography method together with thin layer normal-phase chromatography on the ethyl acetate extract of its entire plant, we isolated four compounds including dioscin (1), b -sitosterol (2), daucosterol (3) and 6'-O-acetyl-b -daucosterol (4). The result of theira-glucosidase inhibitory activity showed that compounds 2 and 4 had strong inhibition with IC50 values of 35.2 and 209.5 mM, comparing to the positive control, acarbose with an IC50 value of 214.5 mM. The results of this study have contributed to the scientific data of chemical compositions of Vietnamese medicinal plants, among them S. procumbens would potentially be developed as a plant-based drug to decrease the blood glucose level.

scholarly journals Two new compounds from leaves of Bruguiera cylindrica (L.) Blume with the in vitro α-glucosidase inhibitory activity

2021 ◽  
Vol 23 (4) ◽  
Author(s):  
Thuy Thi Le Nguyen ◽  
Tung Thanh Bui ◽  
Phung Kim Phi Nguyen ◽  
Chi Minh Tran ◽  
Tu Dang Cam Phan ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Positive Control ◽  
Chemical Structures ◽  
Ic50 Values ◽  
Bruguiera Cylindrica ◽  
New Compounds ◽  
Better Than

Introduction: Bruguiera cylindrica is one of the mangrove plants belonging to Bruguiera genus. This genus is characterized by the presence of a large number of compounds, but the research on bioactivities has not been investigated so far. In the present research, the α-glucosidase inhibitory activity, as well as chemical constituents of the ethyl acetate extract of this plant, were studied. Methods: The chemical structures of two new compounds were elucidated by spectroscopic and computational methods. Results: Two new compounds, benzobrugierol (1) and bruguierine (2), were isolated from leaves of Bruguiera cylindrica (L.) Blume, together with nine known ones, including lupeol (3), betulin (4), chrysoeriol (5), glut-5-ene-3-ol (6), cholesta-4-ene-3-one (7), 3α-(Z)-coumaroyllupeol (8), 3α-(E)-coumaroyllupeol (9), 3β-hydroxycholesta-5-ene-7-one (10) and β-sitosterol 3-O-β-D-glucopyranoside (11). Extracts and some isolated compounds were evaluated for α-glucosidase inhibitory activities. Conclusion: The results showed that most of the extracts and tested compounds exhibited activities better than the positive control acarbose, especially two new compounds 1 and 2 with their IC50 values of 17.9 ± 0.4 and 34.6 ± 0.7 (mg/mL), respectively.

scholarly journals a-GLUCOSIDASE INHIBITORY ACTIVITY AND CHEMICAL CONSTITUENTS OF Satholobus parviflorus (Roxb.)

2011 ◽  
Vol 14 (2) ◽  
pp. 43-49
Author(s):  
Mai Thi Thanh Nguyen ◽  
Trong Nguyen Huu Phan ◽  
Hai Xuan Nguyen ◽  
Nhan Trung Nguyen
Keyword(s):  
Inhibitory Activity ◽  
Protocatechuic Acid ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Positive Control ◽  
Meoh Extract ◽  
Preliminary Screening ◽  
Ic50 Value ◽  
Screening Study ◽  
Ic50 Values

Our preliminary screening study revealed that the methanolic extract of Stholobus parviflorus (Roxb.) exhibited potent a-glucosidase inhibitory activity with an IC50 value of 0.05 μg mL-1. Therefore, we carried out activity-guided fractionation of the MeOH extract and isolated three compounds. These compounds were determinated as ferulic acid (1), protocatechuic acid (2) and epicatechin (3) by analysis of spectroscopic data. The IC50 values of these compounds were 45.30, 23.04 and > 100 μM, respectively. Acid tannic was used as a positive control with an IC50 value of 0.91 nM.

scholarly journals Inhibitory Effects of Secondary Metabolites from the Lichen Stereocaulon evolutum on Protein Tyrosine Phosphatase 1B

Planta Medica ◽  
2021 ◽  
Author(s):  
Birgit Waltenberger ◽  
Françoise Lohézic-Le Dévéhat ◽  
Thi Huyen Vu ◽  
Olivier Delalande ◽  
Claudia Lalli ◽  
...  
Keyword(s):  
Protein Tyrosine Phosphatase ◽  
Inhibitory Activity ◽  
Tyrosine Phosphatase ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Protein Tyrosine Phosphatase 1B ◽  
Protein Tyrosine ◽  
Ic50 Values ◽  
A Cell

AbstractProtein tyrosine phosphatase 1B plays a significant role in type 2 diabetes mellitus and other diseases and is therefore considered a new drug target. Within this study, an acetone extract from the lichen Stereocaulon evolutum was identified to possess strong protein tyrosine phosphatase 1B inhibition in a cell-free assay (IC50 of 11.8 µg/mL). Fractionation of this bioactive extract led to the isolation of seven known molecules belonging to the depsidones and the related diphenylethers and one new natural product, i.e., 3-butyl-3,7-dihydroxy-5-methoxy-1(3H)-isobenzofurane. The isolated compounds were evaluated for their inhibition of protein tyrosine phosphatase 1B. Two depsidones, lobaric acid and norlobaric acid, and the diphenylether anhydrosakisacaulon A potently inhibited protein tyrosine phosphatase 1B with IC50 values of 12.9, 15.1, and 16.1 µM, respectively, which is in the range of the protein tyrosine phosphatase 1B inhibitory activity of the positive control ursolic acid (IC50 of 14.4 µM). Molecular simulations performed on the eight compounds showed that i) a contact between the molecule and the four main regions of the protein is required for inhibitory activity, ii) the relative rigidity of the depsidones lobaric acid and norlobaric acid and the reactivity related to hydrogen bond donors or acceptors, which interact with protein tyrosine phosphatase 1B key amino acids, are involved in the bioactivity on protein tyrosine phosphatase 1B, iii) the cycle opening observed for diphenylethers decreased the inhibition, except for anhydrosakisacaulon A where its double bond on C-8 offsets this loss of activity, iv) the function present at C-8 is a determinant for the inhibitory effect on protein tyrosine phosphatase 1B, and v) the more hydrogen bonds with Arg221 there are, the more anchorage is favored.

scholarly journals Study on α-glucosidase inhibitory activity of 40 kinds of vegetables, tubers, and fruits in An Giang

2021 ◽  
Vol 63 (4) ◽  
pp. 42-46
Author(s):  
Thi-My-Linh Lam ◽  
◽  
Minh-Tuan Le ◽  
Manh-Ha Bui ◽  
◽  
...  
Keyword(s):  
Glycine Max ◽  
Inhibitory Activity ◽  
Positive Control ◽  
Syzygium Cumini ◽  
Inhibition Rate ◽  
Ic50 Value ◽  
Areca Catechu ◽  
Ic50 Values

A study on the α-glucosidase inhibitory activity of 40 kinds of vegetables, tubers, and fruits found in the An Giang province was conducted. The results indicated that all 40 extracted samples displayed α-glucosidase inhibitory activity at a concentration of 250 μg ml-1, 36 extracted samples showed an inhibition rate greater than 50% at 250 μg ml-1, 25 extracted samples had over 50% at 100 μg ml-1, 17 extracted samples possessed more than 50% at 50 μg ml-1, 7 extracted samples sampled showed over 50% at 25 μg ml-1, 5 extracted samples were greater than 50% at 10 μg ml-1, and 1 extracted sample was greater than 50% at 1 μg ml-1. Among the 40 samples, those taken from the seeds of Areca catechu, the fruits of Cassia grandis, the fruits of Syzygium cumini, the seeds of Glycine max,andthe stems of Enydra fluctuansexhibited the strongest α-glucosidase inhibitory activity in methanol, with IC50 values of 0.15, 2.54, 4.05, 5.17 and 8.68 μg ml-1, respectively, which were stronger than the positive control acarbose with an IC50 value of 214.5 μg ml-1

scholarly journals Fingerprinting, Antimicrobial, Antioxidant, Anticancer, Cyclooxygenase and Metabolic Enzymes Inhibitory Characteristic Evaluations of Stachys viticina Boiss. Essential Oil

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3880 ◽  
Author(s):  
Nidal Jaradat ◽  
Nawaf Al-Maharik
Keyword(s):  
Essential Oil ◽  
Therapeutic Potential ◽  
Chemical Constituents ◽  
Metabolic Enzymes ◽  
Positive Control ◽  
Metabolic Enzyme ◽  
Colon Cancer Cell Lines ◽  
Ic50 Values ◽  
Pharmacologically Active

The present study aimed to identify the chemical constituents and to assess the in-vitro, antimicrobial, anticancer, antioxidant, metabolic enzymes and cyclooxygenase (COX) inhibitory properties of essential oil (EO) of Stachys viticina Boiss. leaves. The S. viticina EO was isolated and identified using microwave-ultrasonic and GC-MS techniques, respectively. Fifty-two compounds were identified, of which endo-borneol was the major component, followed by eucalyptol and epizonarene. The EO was evaluated against a panel of in-vitro bioassays. The EO displayed antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Epidermophyton floccosum, with MIC values of 0.039, 0.078 and 0.78 mg/mL, respectively. The EO exhibited cytotoxicity against HeLa (cervical adenocarcinoma) and Colo-205 (colon) cancer cell lines with percentages of inhibition of 95% and 90%, for EO concentrations of 1.25 and 0.5 mg/mL, respectively. Furthermore, it showed metabolic enzyme (α-amylase, α-glucosidase, and lipase) inhibitory (IC50 = 45.22 ± 1.1, 63.09 ± 0.26, 501.18 ± 0.38 µg/mL, respectively) and antioxidant activity, with an IC50 value of 19.95 ± 2.08 µg/mL. Moreover, the S. viticina EO showed high cyclooxygenase inhibitory activity against COX-1 and COX-2 with IC50 values of 0.25 and 0.5 µg/mL, respectively, similar to those of the positive control (the NSAID etodolac). Outcomes amassed from this investigation illustrate that S. viticina EO represents a rich source of pharmacologically active molecules which can be further validated and explored clinically for its therapeutic potential and for the development and design of new natural therapeutic preparations.

scholarly journals Chemical Constituents and Protection of Biodiversity of Corypha taliera Roxb., a Critically Endangered Plant of Bangladesh

2018 ◽  
Vol 20 (2) ◽  
pp. 213-220
Author(s):  
Akhtaruzzaman Chowdhury ◽  
Md Ashraful Alam ◽  
Md Shafiullah Shajib ◽  
Mohammad Abdullah Al Mansur ◽  
Mohammad A Rashid
Keyword(s):  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Pharmaceutical Journal ◽  
Critically Endangered ◽  
Ex Situ ◽  
Endangered Plant ◽  
Separation And Purification ◽  
First Time ◽  
Mature Seeds

This article focuses on the chemical constituents and protection of biodiversity through plantation of saplings of Corypha taliera Roxb., a critically endangered plant of Bangladesh. Until 2010, the tree in the campus of University of Dhaka, used to be considered as the lone surviving species in the world in nature. Succesive chromatographic separation and purification of the methanol extract of air dried flowers of C. taliera provided β-sitosterol (1), β-amyrin (2), and betulinic acid (3) for the first time from its flowers. The structures of these purified compounds were established by extensive spectroscopic analysis and comparison of spectral data with published values as well as co-TLC with authentic samples. On the other hand, 500 mature seeds were sown in seed beds in the Medicinal Plant Garden of Faculty of Pharmacy, University of Dhaka, and Azimpur Government Officers' Quarter premises. After 40 days, the root was first seen to grow in its habitat and 85 days later the shoot developed up to 2.5 cm in height. The rate of germination was found to be 89-93%. The produced saplings were later on planted in different places of Bangladesh for conservation of the plant and protection of biodiversity by ex situ arrangement.Bangladesh Pharmaceutical Journal 20(2): 213-220, 2017

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Daun Alpukat Menggunakan Metode DPPH

2021 ◽  
Vol 11 (2) ◽  
pp. 104-110
Author(s):  
Alfin Surya ◽  
Zaiyar Nazir ◽  
Anggun Syazulfa
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radicals ◽  
Methanol Extract ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values

Free radicals are molecules that lose electrons in their outer orbitals so that the number of electrons becomes odd and unstable. Free radicals can damage various macromolecule cells including proteins, carbohydrates, fats. Antioxidants are compounds that can donate one or more electrons to free radicals. Avocado leaves contain high bioactive components that can be used as a source of natural antioxidants. Avocado leaves that have been dried, mashed and then shifted with methanol. The extracts were tested by phytochemical screening, including flavonpoid, phenolic, tannin and antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhidrazyl) method. This method is based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) against free radicals which causes a change from purple to yellow 2,2-diphenyl-1-picrylhydrazyl) (DPPH). From the research, the IC50 values ​​in avocado leaves were 118.8056 µg / mL and 7,276 µg / mL on ascorbic acid as a positive control. From the IC50 value obtained, it is known that the methanol extract of avocado leaves has strong antioxidant activity to fight free radicals.

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