Mitigation of salt stress in rice plant at germination stage by using methyl jasmonate

To explore the possibility of using methyl jasmonate (MeJA) for alleviation of salt stress, the present study was conducted where six rice varieties (BRRI dhan31, BRRI dhan46, Gota, Kajalsail, Pokkali and Pengek) were grown in non saline (0 dSm-1), saline (12 dSm-1), saline (12 dSm-1) + 10 ?M MeJA and saline (12 dSm-1) + 20 ?M MeJA conditions at germination stage (till the 9 days). MeJA was applied by imbibing seeds in it for 24 hours. To evaluate the effect of MeJA on saline stressed plant at germination stage, change in growth parameters namely germination percentage, shoot and root length, fresh and dry wt. of shoot, fresh and dry wt. of root and biochemical component- activity of alpha-amylase were monitored. Salinity had a minimum effect on final germination percentage (FGP) of rice varieties, but delayed in attaining it. MeJA had least positive effect. Salinity significantly reduced the shoot and root length, fresh and dry weight of all the varieties. MeJA had more decreasing effect in susceptible varieties while increasing effect in moderately tolerant and tolerant. Activity of alpha-amylase in germinated seed varied from 0.051 mg to 0.111 mg mal./mg tissue. At 12dSm-1 salinity, susceptible and moderately tolerant varieties showed significant reduction but tolerant showed significant increase. MeJA intensified the negative effect further in susceptible varieties. In tolerant and moderately tolerant varieties, increment in activity took place and 10 ?M had a better effect over 20 ?M MeJA.Asian J. Med. Biol. Res. March 2016, 2(1): 74-81

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Determination of germination and seedling growth parameters of rice (Oryza sativa L.) varieties under stress conditions

Notulae Scientia Biologicae ◽

10.15835/nsb12310766 ◽

2020 ◽

Vol 12 (3) ◽

pp. 693-701

Author(s):

Fatih ÖNER ◽

Ayşe Ö. Ş. SOYSAL

Keyword(s):

Oryza Sativa ◽

Salt Stress ◽

Growth Parameters ◽

Oryza Sativa L ◽

Germination Rate ◽

Dry Weight ◽

Germination Percentage ◽

Abiotic Factor ◽

Rice Varieties ◽

Stress Levels

Rice (Oryza sativa L.) is an important food for more than half of the world’s population. Globally, rice is grown on approximately 160 million hectares with an average annual production of 740.96 million tonnes. Salinity is an important abiotic factor for germination. In this study rice varieties were analysed for salt stress tolerance at germination growth stage. The response of eight rice varieties against six (0, 25, 50, 75, 100, 150 mM NaCl) salinity levels were studied at germination stage. Seeds of eight rice varieties (‘Yatkin’, ‘Miss-2013 Passali’, ‘Kale’, ‘Manyas Yildizi’, ‘Efe’, ‘Biga Incisi’ and ‘Osmancik-97’) were kept under six salt stress levels. Number of seeds germinated (number), germination rate (%), average germination time, fresh and dry weight of germinated seeds (g), fresh and dry weight of the coleoptile (g), fresh and dry weight of the radicle (g), the length of the coleoptile and radicle (mm) were recorded. The results showed that with increasing salt stress, germination in all the varieties was delayed. The increase in salt stress also reduced every measured trait significantly in all the varieties. Maximum germination percentage (100%) was observed in ‘Biga Incisi’ under all the salt stress levels. Our research data would be helpful for identification of the tolerant varieties which can be studied further in terms of economically.

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Effect of salt stress on some sweet corn (Zea mays L. var. saccharata) genotypes

Archives of Biological Sciences ◽

10.2298/abs150121062s ◽

2015 ◽

Vol 67 (3) ◽

pp. 993-1000 ◽

Cited By ~ 3

Author(s):

Lydia Shtereva ◽

Roumiana Vassilevska-Ivanova ◽

Tanya Karceva

Keyword(s):

Zea Mays ◽

Salt Stress ◽

Zea Mays L ◽

Sweet Corn ◽

Growth Parameters ◽

Dry Weight ◽

Germination Percentage ◽

F1 Hybrid ◽

Salt Stress Condition ◽

Salinity Levels

An experiment was carried out hydroponically under laboratory conditions to investigate the effect of salt stress on several physiological and biochemical parameters of three sweet corn (Zea mays L. var. saccharata) genotypes: lines 6-13, C-6 (pollen source) and their heterotic F1 hybrid ?Zaharina?. The degree of salinity tolerance among these genotypes was evaluated at three different sodium chloride (NaCl) concentrations: 0 mM, 100 mM, 125 mM and 150 mM. Seed germination, plant growth and biochemical stress determining parameters such as malondialdehyde (MDA), proline content and hydrogen peroxide (H2O2) levels were compared between seedlings of lines and hybrid. The obtained results indicated that both lines and hybrid have similar responses at different salinity levels for all examined traits. All the seedlings? growth parameters, such as germination percentage, root length, shoot length, root and shoot fresh and dry weight, decreased with increasing salinity level. MDA, proline and H2O2 increased at different saline conditions in comparison to the control. Based on the results, of the three genotypes examined, the hybrid Zaharina, followed by line C-6, was more salt-sensitive than line 6-13 in salt stress condition.

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Growth Enhancement of Phragmites australis, Eichhornia crassipes and Saccharum officinarum for Rhizoremediation of Crude Oil

International Journal of Environment ◽

10.3126/ije.v7i1.21317 ◽

2018 ◽

Vol 7 (1) ◽

pp. 60-84

Author(s):

Monday Ubogu ◽

Lucky O. Odokuma ◽

Ejiro Akponah

Keyword(s):

Crude Oil ◽

Leaf Area ◽

Root Length ◽

Growth Parameters ◽

Control Plant ◽

Dry Weight ◽

Saccharum Officinarum ◽

Germination Percentage ◽

Growth Enhancement ◽

Plant Soil

P. australis, E. crassipes (in mangrove swamp) and S. officinarum (in rainforest) are capable of tolerating some levels of crude oil in soil. However, some important growth characteristics such speedy growths, extensive root system and increased biomass desirable for efficient rhizoremediation are depressed. To cushion this suppressive effects, plants were subjected to the following treatments: Plant + Soil (PS) (Control); Plant + Soil + Oil (PSO); Plant + Soil + Oil + Fertilizer (PSOF); Plant +Soil + Oil + Fertilizer + Microorganisms (PSOFM); and Plant + Soil + Fertilizer + Microorganisms + Solarization (PSOFMS). Treatments were monitored for 120 days to determine their effects on the following growth parameters: Germination, germination percentage, height, and root length, dry weight, and leaf area. Results indicated that treatments PSOF, PSOFM and PSOFMS enhanced all growth parameters over contaminated untreated soil (PSO) with the exception of germination in P. australis and S. officinarum; while root length, leaf area in E. crassipes were statistically the same for PS, PSO, PSOFM and PSOFMS (P ˂ 0.05). Overall, growth enhancement efficiencies of the applied treatments were in the order: PSOFM ˃ PSOF ˃ PSOFMS. Thus, growth of these plants can be enhanced in crude oil contaminated soil by the above treatments for efficient rhizoremediation.

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Diversity in growth and expression pattern of PoHKT1 and PoVHA transporter genes under NaCl stress in Portulaca oleracea taxa

Genetika ◽

10.2298/gensr1601233e ◽

2016 ◽

Vol 48 (1) ◽

pp. 233-248

Author(s):

R. El-Bakatoushi ◽

A. Elframawy

Keyword(s):

Salt Stress ◽

Plant Growth ◽

Root Length ◽

Growth Parameters ◽

Dry Weight ◽

Significant Negative Correlation ◽

Nacl Stress ◽

Shoot Length ◽

Portulaca Oleracea ◽

Number Of Leaves

Plant growth and the expression of two transporter genes; PoHKT1 and PoVHA transcripts in root and shoot tissues were studied under salt stress of three Portulaca oleracea s.l. taxa. The study showed no significant differences in ratios between root lengths in saline and non-saline treatments of the three taxa, which was correlated with a clear down-regulation of the PoHKT1 transcripts in the root after 150mM NaCl. All measured growth parameters except root length increased in P. oleraceae, decreased in P. granulatostellulata and remain unchanged after 100mM NaCl in P. nitida compared to control under saline conditions. The result was consistent with the type of taxon which had significant effect on the shoot length, number of leaves and dry weight (P< 0.05). All measured growth parameters except root length showed a significant negative correlation with the shoot fold change of PoHKT1 transcripts (r = -0.607, -0.693 and -0.657 respectively). The regulation of PoVHA in root and shoot tissues in the three taxa are significantly different. Under salt stress, both decreased uptake of Na+ into the cytosol by decreasing the expression of PoHKT1 and increased vascular compartmentalization ability of Na+ by inducing the expression of PoVHA seem to work more efficiently in P. oleraceae and P. nitida than in P. granulato-stellulata.

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Effect of Salt Stress on Germination and Growth Parameters of Rice (Oryza sativa L.)

Notulae Scientia Biologicae ◽

10.15835/nsb629163 ◽

2014 ◽

Vol 6 (2) ◽

pp. 237-243 ◽

Cited By ~ 4

Author(s):

Akinbode Foluso OLOGUNDUDU ◽

Adekunle Ajayi ADELUSI ◽

Richard Olutayo AKINWALE

Keyword(s):

Oryza Sativa ◽

Root Length ◽

Dry Matter ◽

Growth Parameters ◽

Oryza Sativa L ◽

Dry Weight ◽

Yield Reduction ◽

Growth Stages ◽

Dry Matter Yield ◽

Rice Varieties

The response of eight varieties of Oryza sativa L. (‘NERICA 1’, ‘NERICA 5’, ‘NERICA 12’, ‘NERICA 19’, ‘IR 29’, ‘IR 20’, ‘IWA 11’, and ‘POKKALI’, a salt tolerant check) against four salinity levels (0, 5, 10, and 15 ds m-1) were studied at germination and early growth stages. Data were analyzed using Statistical Analytical System (SAS) and means were separated by Duncan Multiple Range Test (DMRT) for Final Germination Percentage (FGP), Speed of Germination (SG), Germination Energy Percentage (GE%), shoot and root length and root and shoot dry weight. Based on dry matter yield reduction, rice varieties were classified as tolerant (T), moderately tolerant (MT), moderately susceptible (MS) or susceptible (S). Germination was not recorded at 20 ds m-1 salt concentration in all cultivars. Salinity decreased FGP, SG, GE% and led to reduction in shoot and root length and dry weight in all varieties and the magnitude of reduction increased with increasing salinity stress. Rice varieties ‘NERICA 12’, ‘IR 20’, ‘IWA 11’ and ‘NERICA 19’ showed greater salt tolerance during germination (germinated at 10 ds m-1 salinity). However, ‘NERICA 1’, ‘IR 29’, and ‘IR 20’ performed better based on dry matter yield reduction. The result suggested that ‘NERICA 1’, ‘IR 29’, and ‘IR 20’ might be used for further study of salinity effect on growth processes and physiological consequences at advanced stage of growth. The physiological responses of rice plants to salinity at various developmental stages are therefore critical for identifying salinity tolerance in the cultivars.

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Respon pertumbuhan vegetatif padi varietas IPB 4S pada kondisi cekaman kekeringan

Agromix ◽

10.35891/agx.v11i2.2066 ◽

2020 ◽

Vol 11 (2) ◽

pp. 218-226

Author(s):

Nasrudin Nasrudin ◽

Efrin Firmansyah

Keyword(s):

Drought Stress ◽

Leaf Area ◽

Root Length ◽

Growth Parameters ◽

Field Capacity ◽

Dry Weight ◽

Leaf Color ◽

Rice Varieties ◽

Root Dry Weight ◽

Randomized Design

Drought stress affected growth and decreases rice yield. The use of high yielding varieties and drought tolerance is a strategic step that can be applied during drought stress. The aim of this research was to examine the response of the growth of IPB 4S rice varieties on planting media with drought stress during vegetative phase. The research used a nonfactorial Completely Randomized Design with six levels: field capacity, 10% of field capacity, 20% of field capacity, 30% of field capacity, 40% of field capacity, and 50% of field capacity. The treatments were repeated three times. The growth parameters were plant high, number of tiller, leaf color, leaf area, root length, root dry weight, canopy dry weight, and biomass. The results showed that the drought stress significantly different to leaf area, root length, canopy dry weight, and biomass but were not significantly different to plant height, number of tiller, leaf color, dan root dry weight. Increased of drought stress 30 to 50% of field capacity decreased of leaf area, canopy dry weight, and biomass, but increased root length.

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Infleuence of Vermicompost, Amirthakaraisal and Abda Gold on the Growth of Capsicum annum

Journal of Biotechnology Research ◽

10.32861/jbr.72.18.26 ◽

2021 ◽

pp. 18-26

Author(s):

J. Ketsiyal ◽

A. Joseph Thatheyus

Keyword(s):

Environmental Pollution ◽

Root Length ◽

Growth Parameters ◽

Dry Weight ◽

Germination Percentage ◽

Shoot Length ◽

Total Chlorophyll ◽

Total Chlorophyll Content ◽

Capsicum Annum ◽

Wet Weight

Organic farming has emerged as a crucial priority area globally in view of the growing demand for safe and healthy food and future sustainability and concerns on environmental pollution associated with indiscriminate use of agrochemicals. The effect of vermicompost, amirthakaraisal, and AbdAgold separately and in combination on the growth of Capsicum annum was determined by studying the parameters germination percentage, root length, shoot length, wet weight, dry weight, chlorophyll A, B and total chlorophyll content, and protein content. Application of vermicompost, and amirthakaraisal, in the field, enhance the growth parameters in Capsicum annum.

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Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

Cercetari Agronomice in Moldova ◽

10.2478/cerce-2018-0025 ◽

2018 ◽

Vol 51 (3) ◽

pp. 51-68 ◽

Cited By ~ 1

Author(s):

M.K. Hasan ◽

M.S. Islam ◽

M.R. Islam ◽

H.N. Ismaan ◽

A. El Sabagh

Keyword(s):

Salt Stress ◽

Salinity Tolerance ◽

Seedling Growth ◽

Negative Impact ◽

Germination Rate ◽

Dry Weight ◽

Germination Percentage ◽

Shoot Length ◽

Sand Culture ◽

Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

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GERMINATION AND VIGOUR IN SEEDS OF THE COWPEA IN RESPONSE TO SALT AND HEAT STRESS

Revista Caatinga ◽

10.1590/1983-21252019v32n115rc ◽

2019 ◽

Vol 32 (1) ◽

pp. 143-151 ◽

Cited By ~ 1

Author(s):

Luma Rayane de Lima Nunes ◽

Paloma Rayane Pinheiro ◽

Charles Lobo Pinheiro ◽

Kelly Andressa Peres Lima ◽

Alek Sandro Dutra

Keyword(s):

Salt Stress ◽

Heat Stress ◽

High Temperature ◽

Low Temperature ◽

Vigna Unguiculata ◽

Salt Concentration ◽

Dry Weight ◽

Germination Percentage ◽

Different Types ◽

Different Temperatures

ABSTRACT Salinity is prejudicial to plant development, causing different types of damage to species, or even between genotypes of the same species, with the effects being aggravated when combined with other types of stress, such as heat stress. The aim of this study was to evaluate the tolerance of cowpea genotypes (Vigna unguiculata L. Walp.) to salt stress at different temperatures. Seeds of the Pujante, Epace 10 and Marataoã genotypes were placed on paper rolls (Germitest®) moistened with different salt concentrations of 0.0 (control), 1.5, 3.0, 4.5 and 6.0 dS m-1, and placed in a germination chamber (BOD) at temperatures of 20, 25, 30 and 35°C. The experiment was conducted in a completely randomised design, in a 3 × 4 × 5 scheme of subdivided plots, with four replications per treatment. The variables under analysis were germination percentage, first germination count, shoot and root length, and total seedling dry weight. At temperatures of 30 and 35°C, increases in the salt concentration were more damaging to germination in the Epace 10 and Pujante genotypes, while for the Marataoã genotype, damage occurred at the temperature of 20°C. At 25°C, germination and vigour in the genotypes were higher, with the Pujante genotype proving to be more tolerant to salt stress, whereas Epace 10 and Marataoã were more tolerant to high temperatures. Germination in the cowpea genotypes was more sensitive to salt stress when subjected to heat stress caused by the low temperature of 20°C or high temperature of 35°C.

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ALLELOPATHIC POTENTIAL OF WEED SPEICES AGERATUM CONYZOIDES L. AND CLEOME VISCOSA L. ON GERMINATION AND GROWTH OF SESAMUM INDICUM L

Kongunadu Research Journal ◽

10.26524/krj106 ◽

2015 ◽

Vol 2 (2) ◽

pp. 114-117

Author(s):

Anbarasan R ◽

Prabhakaran J

Keyword(s):

Growth Parameters ◽

Dry Weight ◽

Inhibitory Effect ◽

Germination Percentage ◽

Sesamum Indicum ◽

Weed Species ◽

Ageratum Conyzoides ◽

Allelopathic Potential ◽

Germination And Growth ◽

Cleome Viscosa

Various concentrations (5%, 10%, 15% and 20%) of aqueous extracts prepared from two weed species namely Ageratum conyzoides L. and Cleome viscosa L. and used for the present experiments to determine their allelopathic potential on growth and developmental changes on sesame (Sesamum indicum L.). The weed extracts showed an inhibitory effect on germination percentage, root and shoot growth, and fresh and dry weight of sesame seedlings. The extracts of A.conyzoides had more inhibitory effect at 20%concentration,than that of C.viscosa on growth parameters of sesame.

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