scholarly journals Immediate response of human dental pulp capped with mineral trioxide aggregate, Portland cement and biodentin

2019 ◽  
Vol 45 (2) ◽  
pp. 108-116
Author(s):  
AKM Bashar ◽  
A.K.M Nurul Kabir ◽  
Rozina Akhter Rizdina ◽  
Ranjit Ghosh ◽  
Ashis Kumar Biswas ◽  
...  
Keyword(s):  
Portland Cement ◽  
Inflammatory Reaction ◽  
Dental Pulp ◽  
Mineral Trioxide Aggregate ◽  
Tissue Reaction ◽  
Control Group ◽  
Pulp Capping ◽  
Pulp Inflammation ◽  
Significant Difference ◽  
Human Dental Pulp

Background: The initial inflammatory reaction of pulp capping materials on the dental pulp has an intimate relation in promoting the future cellular differentiation and biomaterial mineralisation. So, analysis of immediate pulpal tissue reaction in vivo, is also important for evaluation of ultimate efficacy any pulp capping agent. To observe immediate inflammatory response of Human Dental Pulp capped with Mineral Trioxide Aggregate (MTA), Biodentin and Portland Cement (PC). Methods: A total of 70 permanent premolars teeth planned to be extracted for orthodontic alignment of occlusion were used as study sample. The teeth were divided into 3 experimental groups, MTA (n=20), Biodentin (n=20) Portland cement (n=20) and control group (n=10). After having an occlusal exposure of approximately 1.5 mm in diameter; in group A, pulp of teeth was capped with 2-mm-thick layer of ProRoot White MTA (Dentsply) and in group B, with sterile Biodentin (Septodont) according to the manufacturer’s recommendations. Whereas in group C, pulp of teeth was capped with sterile Portland Cement (PC). After placing the experimental material in each group, all teeth restored with glass i‹xiomer cement. After 24 hours the teeth were extracted, fixed in 10% buffered formalin solution, then decalcified by 10% nitric acid and embedded in paraffin. Finally, sectioned into 2 to 3-micron-thick serial sections in the linguo-buccal plane and stained with hematoxylin-eosin. After then the amount of pulp inflammation (type, intensity, and extension) were determined by using a predetermined evaluation criterion under an optical microscope at 40a magnification. Ten intact teeth, which received no exposure and pulp capping but extracted due to orthodontic purpose were also collected and treated as the control group (group D); undergone same histologic preparation and evaluation. Significantstatistical differences among the experimental groups were to be found (p<0.05). Results: Histologically, all the three tested materials produced immediate pulpal tissue reaction. ‘Biodentin’ found to be most immediate pulpal tissue reactive (reactive in 100% cases) and ‘Portland Cement’ showed least immediate tissue reaction (only in 30.0% cases). whereas, MTA produced immediate tissue reaction only in 50.0% cases. Immediate pulpal inflammatory reaction in response to tested material found to be statistically significant different between ‘Biodentin’ and ‘Portland cmient’ (p=0.01), also between ‘Biodentin’ and ‘MTA’ and (p=0.001); but there was no statistically significant difference between ‘MTA’ and ‘Portland cement’ (p =0.197). Conclusion: Considering the maximum immediate pulpal tissue reaction (Inflammation), Biodentin is expected to produce most favorable ultimate bioactivity (biomaterial mineralization) after pulp capping. Bangladesh Med Res Counc Bull 2019; 45: 108-116

scholarly journals Histomorphological response of dogs' dental pulp capped with white mineral trioxide aggregate

2004 ◽  
Vol 15 (2) ◽  
pp. 104-108 ◽  
Author(s):  
Italo Medeiros Faraco Junior ◽  
Roberto Holland
Keyword(s):  
Dental Pulp ◽  
Healing Process ◽  
Mineral Trioxide Aggregate ◽  
Morphological Aspect ◽  
Bridge Formation ◽  
Pulp Capping ◽  
Exposure Site ◽  
Pulp Inflammation ◽  
White Mineral

This study was conducted to observe the response of dogs' dental pulp to white mineral trioxide aggregate (MTA) when used as pulp capping material. The pulp of 15 dogs' teeth was experimentally exposed and capped with white MTA. The animals were sacrificed two months later and the specimens were prepared for histomorphological study. The pulp capped with white MTA showed a healing process with complete dentin bridge formation in all samples. In some cases, there was not a tubular dentin shape, but only a structure with an interesting morphological aspect sealing the exposure site. Only 2 specimens exhibited pulp inflammation. In conclusion, the data obtained in this study showed that white MTA has the necessary properties of a pulp capping material.

scholarly journals Enhanced Neurokinin-1 Receptor Expression Is Associated with Human Dental Pulp Inflammation and Pain Severity

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Riffat Mehboob ◽  
Sana Hassan ◽  
Syed Amir Gilani ◽  
Amber Hassan ◽  
Imrana Tanvir ◽  
...  
Keyword(s):  
Pain Score ◽  
Dental Pulp ◽  
Dental Pain ◽  
Receptor Expression ◽  
Strong Positive Correlation ◽  
Pulp Inflammation ◽  
Significant Difference ◽  
Human Dental Pulp ◽  
Neurokinin 1 ◽  
Expression Score

Substance P (SP) is a peptide involved in many biological processes, including nociception and inflammation. SP has a high affinity for its receptor neurokinin-1 (NK-1R). SP/NK-1R complex plays a major role in the interactions going on during the onset of dental pain and inflammation. Objective. To identify the expression of NK-1R in healthy and inflamed human dental pulp, as well as to identify any association with severity of dental pain. Methods. This case-control study included ten irreversibly inflamed samples of dental pulp, which were extirpated from patients presenting with chief complaint of dental pain due to caries. Ten healthy pulps, extirpated from those teeth which were indicated for extraction due to orthodontic reasons, were used as the control group. Visual analog scale (VAS) and modified McGill Pain Questionnaire were used to assess the characteristic and severity of pain. Immunohistochemical study was performed using monoclonal antibodies against NK-1R. Results. The results showed that the NK-1R was expressed intensely in patients with higher pain score. The mean pain score in cases was 7.0 ± 2.0 . The healthy dental pulps had negative or mild NK-1R staining of +1 intensity. The NK-1R score in cases was 2.4 ± 0.516 and 0.2 ± 0.4216 in controls. There was significant difference in NK-1R score between both groups ( p value <0.05). There was a strong positive correlation between the pain score and NK-1R expression score. As the pain increased, the NK-1R expression score was also increased (0.95 ∗ ∗ , p value 0.000). Conclusions. NK-1R is overexpressed in inflamed dental pulp. SP/NK-1R modulation may provide a novel approach for the treatment of pulpal inflammation and pain.

Human Dental Pulp Stem Cells in Rat Mandibular Bone Defects

2019 ◽  
Vol 207 (3-4) ◽  
pp. 138-148 ◽  
Author(s):  
Rubia Teodoro Stuepp ◽  
Priscilla Barros Delben ◽  
Filipe Modolo ◽  
Andrea Gonçalves Trentin ◽  
Ricardo Castilho Garcez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Dental Pulp ◽  
Bone Defects ◽  
Treated Group ◽  
Dental Pulp Stem Cells ◽  
Control Group ◽  
Mandibular Bone ◽  
Significant Difference ◽  
Human Dental Pulp

This study aimed to evaluate the use of human dental pulp stem cells (hDPSCs) in non-critical-sized mandibular bone defects in rats. hDPSCs from permanent teeth were isolated and engrafted in mandibular bone defects in rats for 7, 14, and 28 days; bone defects without cells formed the control group. Samples were evaluated by scanning electron microscopy (SEM), light microscopy (hematoxylin and eosin staining), and the regeneration area was measured by the Image J program. Before surgery procedures, the human dental pulp cells were characterized as dental pulp stem cells: fusiform morphology, plastic-adherent; expression of CD105, CD73, and CD90; lack of expression of CD45 and CD34, and differentiated into osteoblasts, adipocytes, and chondroblasts. The results indicated that within 7 days the control group presented a pronounced bone formation when compared with the treated group (p < 0.05). After 14 days, the treated group showed an increase in bone formation, but with no statistical difference among the groups (p > 0.05). In the final evaluated period there was no difference between the control group and the treated group (p > 0.05). There was a significant difference between 7 and 14 days (p < 0.05) and between 7 and 28 days (p < 0.05) in the treated group. In conclusion, there is no evidence that the use of hDPSCs in the conditions of this study could improve bone formation in non-critical-sized mandibular bone defects.

scholarly journals THE EFFECT OF COMBINATION CALCIUM HYDROXIDE-PROPOLIS APPLICATION TO AMOUNT OF NEUTROFIL CELLS ON WISTAR RAT PULP PERFORATION

2020 ◽  
Vol 10 (2) ◽  
pp. 66
Author(s):  
Ira Widjiastuti ◽  
Setyabudi Setyabudi ◽  
Nia Nur Haliza
Keyword(s):  
Calcium Hydroxide ◽  
Wistar Rats ◽  
Healing Process ◽  
Wistar Rat ◽  
Combination Group ◽  
Control Group ◽  
Pulp Capping ◽  
Pulp Inflammation ◽  
Significant Difference ◽  
The Difference

Background: Pulp inflammation can be treated with pulp capping by calcium hydroxide as the gold standard. Pulp capping by calcium hydroxide could causing tissue necrosis followed by inflammation. Calcium hydroxide combined with propolis which have anti-inflammatory effects through inhibition of the Nf-kB pathway and pro-inflammatory cytokines. That process will inhibit the increase of vascular permeability and neutrophil chemotaxis. Inflammatory process can accelerate by controlling the amount of neutrophil cells as a acute phase marker, so the healing process occurs more quickly. Therefore it is necessary to study the amount of neutrophil cells in the pulp perforation of Wistar rats after applicated by combination of calcium hydroxide-propolis. Purpose: To analyze the difference amount of neutrophil cells on the Wistar rats pulp perforation after applicated by calcium hydroxide-propolis combination compared with calcium hydroxide-aquadest Method: M1 tooth perforated for all groups with three types tratment: no medication (control group), calcium hydroxide-aquadest 1: 1 (P-1 group), and calcium hydroxide-propolis 1: 1.5 (P-2 group) and closed with cention. Observations were made on the 1st, and 3rd day with HE staining. Results: One Way ANOVA shown a significant difference (p <0.05) of the average amount of neutrophil cells between all treatment groups on 1st day (p=0,000) and 3rd day (p=0,000). The amount of neutrophil cells in the calcium hydroxide-propolis (P-2) combination group was lower than control group and calcium hydroxide-aquadest (P-1) group on the 1st and 3rd day. Conclusion: The amount of neutrophil cells after application of calcium hydroxide-propolis is lower than calcium hydroxide-aquadest application.

scholarly journals THE EFFECT OF KARAMUNTING (Rhodomyrtus Tomentosa) LEAF EXTRACT ON THE NUMBER OF MACROPHAGES IN PULP INFLAMMATION

2021 ◽  
Vol 6 (2) ◽  
pp. 131
Author(s):  
Indah Septiani ◽  
Muhammad Yanuar Ichrom Nahzi ◽  
Nolista Indah Rasyid
Keyword(s):  
Calcium Hydroxide ◽  
Dental Pulp ◽  
Leaf Extract ◽  
Simple Random Sampling ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Not Given ◽  
Pulp Inflammation ◽  
Significant Difference

Background: Reversible pulpitis is an inflammation of dental pulp caused by the opening of the pulp due to cavities. One of the procedures in exposed pulp treatment is direct pulp capping using calcium hydroxide. However, this material has side effects, its high pH can cause necrosis, and due to that, a safer alternative material is needed. Karamunting leaf extract contains phenolic compounds, flavonoids, tannins, and saponins which have immunomodulatory properties that play an important role in healing exposed pulp. Objective: To determine the effect of karamunting leaf extract on the number of macrophages in pulp inflammation. Methods: This was a true experimental research with posttest-only group design, using simple random sampling that consist of 24 male Wistar rats which later be divided into 3 groups. The perforated rat dental pulp was then treated with karamunting leaf extract as a treatment group, calcium hydroxide as a positive control group, and not given any application (without drug) as a negative control group. The samples were analyzed histologically on the 3rd to 7th day after the application, inflammatory response occurred in all groups. Results: The two-way ANOVA results showed that there was a significant difference between the karamunting leaf extract group, the group that was not given drug, and the group given calcium hydroxide with a value p<0.05. Conclusion: Based on the research conducted, it is concluded that the administration of karamunting leaf extract can reduce the number of macrophages in pulp inflammation. Keywords: calcium hydroxid, exposed pulp, karamunting leaf, macrophages, pulp inflammation.

Comparison of Chemical Composition between Indonesian White Portland Cement and MTA as Dental Pulp Capping Material

2019 ◽  
Vol 829 ◽  
pp. 34-39
Author(s):  
Denny Nurdin ◽  
Indra Primathena ◽  
Rahmi Alma Farah ◽  
Arief Cahyanto
Keyword(s):  
Chemical Composition ◽  
Portland Cement ◽  
Dental Pulp ◽  
Functional Group ◽  
Mineral Trioxide Aggregate ◽  
Pulp Capping ◽  
Dental Pulp Capping ◽  
Pulp Exposure ◽  
And Control ◽  
White Portland Cement

Pulp capping has been suggested as one treatment of choice after pulp exposure. Calcium hydroxide [Ca (OH)2] recognized as gold standard of direct pulp capping matter for some decades. Throughout the time, a new cement known as mineral trioxide aggregate (MTA) has developed into a prominent alternative. A recent study found that matter as the most effective pulp capping material. Despite the fact, MTA is an expensive material. Several studies in different country show that portland cement has highly similarity chemical composition with MTA. The purpose of this study is to evaluate the chemical composition of white portland cement that fabricated in Indonesia compared to MTA. White portland cement fabricated in Indonesia was used as a sample in this study and commercial MTA as a control. Samples and control were assessed using X-ray fluorescence spectrometry (XRF) to figure out chemical composition and concentration. Investigating the wavelength of the functional group using Fourier Transform Infrared Spectroscopy (FTIR). The results show that composition, concentration, and functional group either Indonesian white portland cement and MTA has highly similar. Hence, it has a chance to use Indonesian white portland cement for dental pulp capping material as MTA substitution.

scholarly journals Increased VEGF-A Expression of Human Dental Pulp Stem Cells (hDPSCs) Cultured with Advanced Platelet Rich Fibrin (A-PRF)

2021 ◽  
Vol 15 (1) ◽  
pp. 569-574
Author(s):  
Dini Asrianti Bagio ◽  
Indah Julianto ◽  
Anggraini Margono ◽  
Endang Suprastiwi
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Control Group ◽  
Platelet Rich Fibrin ◽  
Significant Difference ◽  
Angiogenesis Process ◽  
Human Dental Pulp ◽  
Post Hoc

Background: VEGF-A expression of human dental pulp stem cells (hDPSCs) can induce the angiogenesis process of dental pulp regeneration. This in vitro study aimed to analyze the effect of various concentrations of Advanced Platelet Rich Fibrin (A-PRF) conditioned media (CM) on the increased expression of vascular endothelial growth factor-A (VEGF-A) of hDPSCs. Methods: hDPSCs were collected from ten third molars extracted from nine healthy donors, cultured, and then harvested at the end of the third passage. The hDPSCs were seeded in four different CM (control group: hDPSCs + DMEM; 1% A-PRF CM group: hDPSCs + 1% A-PRF CM; 5% A-PRF CM group: hDPSCs + 5% A-PRF CM; 10% A-PRF CM group: hDPSCs + 10% A-PRF CM). All of the groups were cultured in biological triplicates (Triplo) and observed for 5, 12, and 24 hours. The VEGF-A protein expression of hDPSCs was measured using human VEGF-A ELISA at a wavelength of 405 nm. Data was analyzed with Kruskal Wallis and post hoc Mann Whitney test with p<0.05. Results: The VEGF-A expression rate of hDPSCs among all groups was statistically significantly different at 5, 12 and 24 hours of observations (p<0.05). Post hoc analysis test showed a statistically significant difference of hDPSCs’s VEGF-A expression between 5% A-PRF groups compared to other groups at 5 and 12 hours of observation (p<0.05). However, there were no statistically significant differences observed of hDPSCs’ VEGF-A expression at 24 hours of observation between 1%, 5% and 10% A-PRF groups (p>0.05). Conclusion: 5% A-PRF CM was superior in increasing VEGF-A expression of hDPSCs at 5, 12 and 24 hours of observations.

scholarly journals THE INFLAMMATORY REACTION OF PULP TISSUE WITH CALCIUM HYDROXIDE, MTA AND PORTLAND CEMENT (In Vivo)

2017 ◽  
Vol 4 (2) ◽  
pp. 114
Author(s):  
Jos Erry Hesti Witasari ◽  
Usman Munyati ◽  
Anggraini Dewi
Keyword(s):  
Portland Cement ◽  
Calcium Hydroxide ◽  
Inflammatory Reaction ◽  
Mineral Trioxide Aggregate ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Posterior Teeth ◽  
Capping Agents ◽  
Pulp Capping

Background : Perforation in normal pulp could be happened anytime. In sterile condition, direct pulp capping was the right therapy to maintain the vitality and pulp function. Direct pulp capping agent must contact with the pulp tissue. Generally, body had an immunologic respond with foreign body that contact with tissue. The respond could be inflammatory reaction. The used direct pulp capping agents were calcium hydroxide, and Mineral Trioxide Aggregate (MTA) which known as better agent but relatively expensive and difficult to obtain. So that, the alternative of MTA, Portland cement which was the same essential of MTA, was being developed. The aim of this study, to analized inflammatory reaction of the pulp tissue with direct pulpcapping agents calcium hydroxide, MTA, and Portland cement.Methods: Free caries M. Nemestrina’s posterior teeth were prepared to formpin point perforations on buccal surface of the crowns. The teeth were appliedwith one of the three capping agents. Teeth were filled and extracted. Theextracted teeth were proceed into histopatological preparation slides to evaluatethe inflammatic reaction.Results: The result showed no statistically significant differences of pulp inflammatory reaction between calcium hydroxide, MTA and Portland cement in7, 14, 42, and 90 days.Conclusion: The inflammatory reaction of pulp tissue of the three pulp cappingagent (calcium hydroxide, MTA, and PC) were insignificant different.

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