scholarly journals In vitro Thrombolytic and Membrane Stabilizing Studies of Brassica rapa subsp. chinensis (L.) Hanelt

2015 ◽  
Vol 17 (2) ◽  
pp. 172-176 ◽  
Author(s):  
Nushrat Jahan ◽  
SM Ashikur Rahman ◽  
Mohammad S Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Brassica Rapa ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Negative Control ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Standard Drug ◽  
Crude Methanol Extract

Brassica rapa subsp. chinensis (L.) Hanelt is a popular herb in Bangladesh. In the present studies, the plant has been assessed to evaluate its in vitro thrombolytic and membrane stabilizing properties. Initially, the plant was collected, processed and extracted with methanol and the concentrated crude methanol extract was fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble materials. All extractives, including the parent extract were subjected to thrombolytic and membrane stabilizing bioassays. In case of thrombolytic study, the carbon tetrachloride soluble fraction exhibited highest clot lysis. Here, streptokinase and distilled water were used as positive and negative control, respectively. However, in the assay for membrane stabilizing activity, the crude methanol extract along with other extractives were capable to inhibit hemolysis of erythrocyte membrane in hypotonic solution- and heat- induced conditions, which indicated anti-inflammatory property of the samples. Acetyl salicylic acid was used as standard drug in this assay. This represents the first report of thrombolytic and membrane stabilizing activities of B. rapa subsp. chinensis. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22336 Bangladesh Pharmaceutical Journal 17(2): 172-176, 2014

scholarly journals In vitro Antioxidant, Membrane Stabilizing and Thrombolytic Activities of Glycosmis arborea

2012 ◽  
Vol 15 (2) ◽  
pp. 141-143 ◽  
Author(s):  
Sayed Mamur Hossain ◽  
Farhana Islam ◽  
Tasnuva Sharmin ◽  
Hasib Sheikh ◽  
AM Rashedul Hasan ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Rat Erythrocytes ◽  
Thrombolytic Activity ◽  
Negative Controls

The methanol extract of leaves and stems of Glycosmis arborea and their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to assays for antioxidant activity by Folin-Ciocalteau reagent, membrane stabilizing and thrombolytic activities. The carbon tetrachloride soluble fraction of stems of G. arborea demonstrated the presence of significant amount of phenolic compounds (36.95 ± 0.54 mg of GAE/g of extract). The extractives inhibited heat as well as hypotonic solution-induced haemolysis of rat erythrocytes in vitro. The pet-ether soluble fraction of leaves and stems of G. arborea showed 20.46 and 38.24% and 22.50 and 48.25%. inhibition of hemolysis of RBC caused by heat and hypotonic solution, as compared to 30.55 and 72.91% inhibition of hemolysis of RBC caused by the standard Acetyl salicylic acid at 0.01 mg/ml concentration, respectively. Among the four fractions, the chloroform soluble materials of the stems of G. arborea revealed highest thrombolytic activity with clot lysis value of 36.50% while standard streptokinase and water used as positive and negative controls, showed 64.25±0.26 and 2.35±0.35 % lysis of clot respectively DOI: http://dx.doi.org/10.3329/bpj.v15i2.12579 Bangladesh Pharmaceutical Journal 15(2): 141-143, 2012

scholarly journals Membrane Stabilizing and Preliminary Hypoglycemic Activities of Picrasma javanica

2013 ◽  
Vol 16 (1) ◽  
pp. 89-92
Author(s):  
Tasnuva Sharmin ◽  
Farhana Islam ◽  
Md. Al Amin Sikder ◽  
Shaila Kabir ◽  
Mohammad Rashedul Haque ◽  
...  
Keyword(s):  
Body Weight ◽  
Salicylic Acid ◽  
Carbon Tetrachloride ◽  
Blood Sugar ◽  
Blood Sugar Level ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Hypotonic Solution ◽  
Standard Drug ◽  
Crude Methanol Extract

The methanol extract of leaves of Picrasma javanica Blume and its n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were evaluated for membrane stabilizing and preliminary hypoglycemic activities. In hypotonic solution and heat-induced conditions, the crude methanol extract inhibited 65.30±0.52% and 36.79±0.38% haemolysis of RBC as compared to 72.79% and 42.12% produced by standard drug, acetyl salicylic acid, respectively (0.10 mg/ml). The crude extract also showed blood sugar lowering efficacy at 200 and 400 mg/ kg body weight, with 48.63% reduction of blood sugar level at 400 mg/kg body weight while the standard drug, Glibenclamide (10 mg/kg body weight) demonstrated 53.7% reduction of blood sugar level at 3rd hour of administration. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14500 Bangladesh Pharmaceutical Journal 16(1): 89-92, 2013

scholarly journals In vitro Cytotoxic, Membrane Stabilizing and Thrombolytic Activities of Polygonum glabrum Willd

2015 ◽  
Vol 17 (2) ◽  
pp. 202-204 ◽  
Author(s):  
Mohammad Firoz Khan ◽  
Sikder Nahidul Islam Rabbi ◽  
Fahima Aktar ◽  
Md Hassan Kawsar
Keyword(s):  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
The Other ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Activity Assay ◽  
Thrombolytic Activity ◽  
Lc50 Value ◽  
Crude Methanol Extract

The crude methanol extract of leaves of Polygonum glabrum Willd and its Kupchan fractions were screened for cytotoxic, membrane stabilizing and thrombolytic activities. Among all fractions, the crude methanol extract showed significant cytotoxic activity having LC50 value 0.74 ± 0.045 ?g/ml. Moreover, in hypotonic solution- and heat- induced conditions, the crude methanol extract inhibited hemolysis of human erythrocyte by 79.21 ± 0.44% and 84.87±0.23%, respectively as compared to 71.9 ± 0.73% and 42.12 ± 0.37% demonstrated by the standard acetyl salicylic acid. On the other hand, in thrombolytic activity assay the methanol extract demonstrated highest clot lysis value of 35.17 ± 0.42%. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22341 Bangladesh Pharmaceutical Journal 17(2): 202-204, 2014

scholarly journals Membrane Stabilizing and Thrombolytic Activities of Sida rhombifolia L.

2015 ◽  
Vol 17 (1) ◽  
pp. 43-45
Author(s):  
Mohammd Firoz Khan ◽  
Sikder Nahidul Islam Rabbi ◽  
Md Aslam Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Salicylic Acid ◽  
Human Erythrocyte ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Thrombolytic Activity ◽  
Whole Plant ◽  
Crude Methanol Extract ◽  
Sida Rhombifolia

The crude methanol extract of whole plant of Sida rhombifolia and different fractions generated from it were tested for membrane stabilizing and thrombolytic activities. In hypotonic solution- and heat-induced conditions, the chloroform soluble partitionates inhibited haemolysis of human erythrocyte by 22.11±0.14% and 64.71±0.08%, respectively as compared to 71.97±0.51% and 40.12±0.29% demonstrated by the standard acetyl salicylic acid (ASA). Moreover, the pet ether soluble materials revealed highest thrombolytic activity with clot lysis value of 19.51±0.03% as compared to 66.98±0.11% exhibited by the standard streptokinase. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22313 Bangladesh Pharmaceutical Journal 17(1): 43-45, 2014

scholarly journals In vitro Antioxidant, Cytotoxic and Membrane Stabilizing Activities of Bauhinia acuminata L.

2015 ◽  
Vol 17 (1) ◽  
pp. 99-101
Author(s):  
Mohammad Firoz Khan ◽  
Rabeya Islam Shilpi ◽  
Ridwan Bin Rashid ◽  
Mohammad A Rashid
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
The Other ◽  
Hypotonic Solution ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Crude Methanol Extract

The crude methanol extract of leaves of Bauhinia acuminata and its Kupchan fractions were screened for antioxidant, cytotoxic and membrane stabilizing activities. Among all partitionates the aqueous soluble fraction of B. acuminata demonstrated the highest antioxidant activity with IC50 value of 7.22±0.200 ?g/ml. Moreover, the carbon tetrachloride soluble fraction showed significant cytotoxic activity having LC50 value of 12.13±0.215 ?g/ml. On the other hand, in hypotonic solution- and heat- induced conditions, the crude methanol extract inhibited haemolysis of human erythrocyte by 63.94±0.14% and 51.95±0.20%, respectively as compared to 81.97±0.77% and 42.11±0.39% demonstrated by the standard acetyl salicylic acid. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22324 Bangladesh Pharmaceutical Journal 17(1): 99-101, 2014

scholarly journals Bioactivities of Artocarpus chaplasha Roxb. and Bougainvillea spectabillis Willd.

2013 ◽  
Vol 16 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Farjana Chowdhury ◽  
Senjuti Pal ◽  
Tasnuva Sharmin ◽  
Ridwan Bin Rashid ◽  
Md. Al Amin Sikder ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Crude Extract ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Clot Lysis ◽  
Total Phenolic ◽  
Hypotonic Solution ◽  
Lysis Activity ◽  
Crude Methanol Extract

The methanol extracts of leaf of Artocarpus chaplasha Roxb. and Bougainvillea spectabillis Willd. as well as their petroleum ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to screenings for antioxidant, cytotoxic, thrombolytic, membrane stabilizing and antimicrobial activities. The antioxidant potential was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin- Ciocalteau reagent using butylated hydroxytolune (BHT) and ascorbic acid as standards. The aqueous soluble fraction of the crude methanol extract of A. chaplasha and the methanolic crude extract of B. spectabillis demonstrated the highest free radical scavenging activity with the IC50 of 15.60±0.27 ?g/ml and 18.33±0.17 ?g/ml, which could be correlated to their total phenolic content of 61.26±0.23 and 50.00±0.19 mg of GAE/gm of extractive, respectively. Different extractives of A. chaplasha and B. spectabillis showed clot lysis activity ranging from 2.56±0.68% to 17.07±0.23% and 2.24±0.71% to 17.59±0.74% as compared to 66.77% and 3.791 % produced by the standard streptokinase and water, respectively. In hypotonic solution and heat induced conditions, the crude extract of A. chaplasha inhibited the haemolysis of human erythrocyte by 41.96±0.87% and 40.00±0.78%, while the carbon tetrachloride soluble fraction of methanol extract of B. spectabillis inhibited haemolysis by 65.68±0.36% and 35.62±0.82%, respectively. Here, acetyl salicylic acid (0.1 mg/ml) was used as reference showing 72.79% and 42.12% of haemolysis of RBCs in hypotonic and heat induced conditions, respectively. Among the extractives of both plants, the crude methanol extract of A. chaplasha and the chloroform soluble fraction of B. spectabillis extract revealed mild to moderate antimicrobial activity with zone of inhibition ranging from with 7.0 to 13.0 mm. The general toxicity was determined by brine shrimp lethality bioassay where the pet-ether soluble fraction of A. chaplasha (LC50 0.781±0.36 ?g/ml) and the aqueous soluble partitionate of B. spectabillis (LC50 1.28±0.57 ?g/ml) suggested the presence of considerable bioactive principles. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14493 Bangladesh Pharmaceutical Journal 16(1): 63-68, 2013

scholarly journals Pharmacological Activities of Senna obtusifolia Linn.: A Medicinal Plant of Bangladesh

2015 ◽  
Vol 17 (2) ◽  
pp. 182-186 ◽  
Author(s):  
Md Anisuzzaman ◽  
Md Qamrul Ahsan ◽  
Md Ruhul Kuddus ◽  
Mohammad Abdur Rashid
Keyword(s):  
Plant Extract ◽  
Pharmaceutical Journal ◽  
Anxiolytic Activity ◽  
Hypotonic Solution ◽  
Pharmacological Activities ◽  
Senna Obtusifolia ◽  
Inhibitory Potential ◽  
Dose Dependent ◽  
Crude Methanol Extract

The crude methanol extract of seed of Senna obtusifolia Linn. has been investigated for anxiolytic, antiatherothrombosis, membrane stabilizing and alpha-amylase inhibitory activities. The anxiolytic activity was examined in mice by using the hole cross and open field test (OFT). The anti-atherothrombosis activity was evaluated and compared with that of standard streptokinase. The membrane stabilizing activity was tested by using hypotonic solution- and heat-induced hemolysis of human erythrocyte. The plant extract was also assessed for anti-diabetic activity through in vitro ?-amylase inhibitory potential. The ?-amylase inhibitory activity of S. obtusifolia was measured using the starch-iodine method. The crude extract of S. obtusifolia showed moderate anxiolytic activity. In the in-vitro anti-atherothrombosis test, the extract exhibited mild activity as compared to the standard, streptokinase (81.53%). In membrane stabilizing activity test, the plant extract at 1.0 mg/ml inhibited the heat-induced hemolysis of RBCs by 56.37% whereas the standard acetyl salicylic acid (ASA) demonstrated 71.36% inhibition of hemolysis. Our results revealed that the extract produced dose-dependent prevention of digestion of carbohydrates by inhibiting ?-amylase. These findings demonstrated that S. obtusifolia may be a good candidate for further analysis because of its effective pharmacological properties. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22338 Bangladesh Pharmaceutical Journal 17(2): 182-186, 2014

scholarly journals Bioactivities of Malvaviscus arboreus var. drummondii and Phyllanthus reticulatus Poir.

2015 ◽  
Vol 13 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Zokhroof Yeasmin ◽  
Sharif Tanvir ◽  
Tasnuva Sharmin ◽  
Ridwan Bin Rashid ◽  
Md Al Amin Sikder ◽  
...  
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Activity Assay ◽  
Vincristine Sulphate ◽  
Crude Methanol Extract

The study was designed to evaluate the bioactivities of crude methanol extracts of leaves of Malvaviscus arboreus and Phyllanthus reticulatus along with their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates. The antioxidant potential was evaluated by DPPH and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. In the DPPH free radical scavenging assay, the crude methanol extract of M. arboreus revealed the highest free radical scavenging activity with IC50 values 6.47 ± 0.78 ?g/ml. In brine shrimp lethality bioassay, the chloroform soluble materials of M. arboreus displayed the highest cytotoxic potential having LC50 values 2.73 ± 0.36 ?g/ml, whereas the standard vincristine sulphate exhibited an LC50 value of 0.45 ?g/ml. In the thrombolytic activity assay, the pet-ether soluble fractions of M. arboreus and P. reticulatus revealed 55.60 ± 0.48 % and 18.99 ± 0.84% clot lysis, respectively. In the membrane stabilizing activity assay, the chloroform soluble fraction of M. arboreus inhibited heat- and hypotonic solution- induced haemolysis of RBCs by 54.60 ± 0.81 % and 66.96 ± 0.84 % as compared to 57.06 % and 72.79 % inhibition by acetyl salicylic acid (0.10 mg/ml), respectively. On the other hand, the pet-ether soluble materials of crude methanol extract of P. reticulatus demonstrated 57.11 ± 0.57 % and 70.62 ± 0.22 % inhibition of heat- and hypotonic solution- induced hemolysis, respectively. DOI: http://dx.doi.org/10.3329/dujps.v13i2.21892 Dhaka Univ. J. Pharm. Sci. 13(2): 143-147, 2014 (December)

scholarly journals Thrombolytic Activity of Methanolic Extracts of Desmodium paniculatum (L.) and Sarcochlamys pulcherrima (Roxb.)

2015 ◽  
Vol 17 (1) ◽  
pp. 67-69 ◽  
Author(s):  
Mohammed Aktar Sayeed ◽  
Humayun Kabir ◽  
Mohammad Mamun Ur Rashid ◽  
Md Farid Ahamad Bhuiyan ◽  
Mohammad A Rashid
Keyword(s):  
Pharmaceutical Journal ◽  
Positive Control ◽  
Negative Control ◽  
Clot Lysis ◽  
Herbal Extracts ◽  
Thrombolytic Activity ◽  
Methanolic Extracts ◽  
Desmodium Paniculatum

An in vitro thrombolytic model was used to check the clot lysis effect of two herbal extracts viz., Desmodium paniculatum (L.) and Sarchochlamys pulcherrima (Roxb.) by using Streptokinase as positive control and water as negative control. D. paniculatum and S. pulcherrima showed 31.92 ± 8.09% and 36.12 ± 6.81% clot lysis, respectively. From our study we found that D. paniculatum and S. pulcherrima showed significant % of clot lysis effect with reference to Streptokinase (72.54 ± 6.03%). and water (3.48 ± 0.84%). DOI: http://dx.doi.org/10.3329/bpj.v17i1.22318 Bangladesh Pharmaceutical Journal 17(1): 67-69, 2014

scholarly journals Clot Lysis and Antimitotic Study of Ficus glomerata Roxb Fruit Extracts

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Kirankumar Shivasharanappa ◽  
Ramesh Londonkar
Keyword(s):  
Petroleum Ether ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Negative Control ◽  
Assay Method ◽  
Clot Lysis ◽  
Distilled Water ◽  
Antimitotic Activity ◽  
Ficus Glomerata

The present study was carried out to investigate the thrombolytic and antimitotic potentiality of various extracts of fruits of Ficus glomerata, a traditional medicinal plant, using an in vitro assay method. Three crude extracts such as petroleum ether (FGPE), chloroform (FGCE), and methanol (FGME) were used for the study, with a standard (streptokinase) and negative control (sterile distilled water) to validate the method. The thrombolytic nature of the plant was found significant with methanol extract and chloroform and petroleum ether extracts have recorded mild activity, when compared with the negative control (sterile distilled water). The extracts have shown mild clot lysis, that is, 2.16%, 23.06%, 27.60%, and 47.74% of sterile distilled water, FGPE, FGCE, and FGME, respectively, while the standard (streptokinase) has shown 74.22% clot lysis. FGME inhibited the root growth in number as well as length effectively, followed by FGPE, while FGCE exhibited moderate antimitotic activity and it was supported by mitotic index. Therefore, the obtained results suggest that among all the extracts of plant the methanolic extract has shown highest thrombolytic and antimitotic activity.

Sign in / Sign up

Export Citation Format

Share Document