scholarly journals In vitro Propagation of Albizia guachapele, Cedrela odorata, Platymiscium pinnatum and Guaiacum sanctum

1970 ◽  
Vol 18 (2) ◽  
pp. 151-156
Author(s):  
L. Valverde-Cerdas ◽  
A. Rojas-Vargas ◽  
A. Hine-Gómez
Keyword(s):  
Costa Rica ◽  
Key Words ◽  
Plant Species ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Cedrela Odorata ◽  
Resistant Material ◽  
In Vitro Micropropagation ◽  
Guaiacum Sanctum

In Costa Rica Albizia guachapele, Cedrela odorata, Platymiscium pinnatum and Guaiacum sanctum are important plant species in both economic and ecological terms and their wood is precious and reported to be highly resistant material. This research has evaluated the in vitro micropropagation as a technology focused to conserve these species. Findings include percentage of germination of seeds and contamination, induction of buds, rooting and growth of micro-cuttings of these four species. Key words:  In vitro, Propagation, Albizia guachapele, Cedrela odorata, Platymiscium pinnatum, Guaiacum sanctum D.O.I. 10.3329/ptcb.v18i2.3397 Plant Tissue Cult. & Biotech. 18(2): 151-156, 2008 (December)

scholarly journals Effects of Growth Regulators on In vitro Propagation and Tuberization of Four Dioscorea Species

1970 ◽  
Vol 18 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Md. Tariqul Islam ◽  
E. R. Joachim Keller ◽  
D. Philibert Dembele
Keyword(s):  
Key Words ◽  
Growth Regulators ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
In Vitro Production ◽  
Dioscorea Species ◽  
Vitro Production ◽  
Species Being ◽  
Nodal Culture

Nodal explants of 12 accessions from four species of yam (Dioscorea spp.) were cultured for six weeks on MS to evaluate the influence of IAA, Kn, NAA and BAP on the production of leaves and microtubers. Four Dioscorea polystachya Turcz., three each of D. bulbifera L. and D. sansibarensis Pax. and two D. japonica Thunb. accessions were used. Five and 10 mg/l of Kn along with IAA and sucrose, and 0.2 and 0.5 mg/l of NAA, sucrose and with or without BAP were used in four treatments. The accessions Yam 23 and Yam 25 of D. sansibarensis failed to initiate any leaf under four treatments. The remaining accessions produced 0.11 to 1.76 leaves per explant. The medium containing IAA with higher concentration of Kn (10 mg/l) and 3% sucrose was found to be best for in vitro production of leaf (0.71/explant) and the most productive species was D. japonica (1.36), followed by D. polystachya (1.19/explant). At the same culture period, Yam 16 of D. bulbifera failed to initiate any microtuber at IAA with Kn, and NAA with or without BAP. The remaining accessions produced 0.09 to 1.15 microtubers per explant. Lower concentration of Kn (5 mg/l) with IAA and sucrose was favourable for producing microtubers (0.61/explant on an average), the best species being D. sansibarensis (1.27) followed by D. japonica (0.59/ explant). Finally, the presence of BAP adversely affected the production of microtuber among Dioscorea species. Key words: Dioscorea spp., Microtuber, Nodal culture, Propagation D.O.I. 10.3329/ptcb.v18i1.3260 Plant Tissue Cult. & Biotech. 18(1): 25-35, 2008 (June)

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals In vitro Propagation and Ex vitro Rooting of Blueberry Plantlets

1970 ◽  
Vol 18 (2) ◽  
pp. 187-195 ◽  
Author(s):  
Zhao Guang-jie ◽  
Wang Zhan-bin ◽  
Wang Dan
Keyword(s):  
Tissue Culture ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Root Formation ◽  
Early Stage ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Ex Vitro

Effects of different concentrations of 2-ip and IBA in WPM basal medium for Blomidon blueberry in vitro propagation and four different rooting agents at the early stage after transplantation showed that 15 mg/l of 2-ip is the best concentration to induce shoots. For optimum in vitro root formation 10 µM IBA was found to be best and four rooting agents for seedling transplantation according to their effects were No.2>, No.4>, No.3 >, water > and No. 1. Key words: Blomidon, Tissue culture, In vitro regeneration, Rooting agent D.O.I. 10.3329/ptcb.v18i2.3650 Plant Tissue Cult. & Biotech. 18(1): 187-195, 2008 (December)

scholarly journals In vitro Propagation of Bitter Gourd (Momordica charantia L.) from Nodal and Root Segments

1970 ◽  
Vol 19 (1) ◽  
pp. 45-52 ◽  
Author(s):  
M.A.Z. Al Munsur ◽  
M.S. Haque ◽  
K.M. Nasiruddin ◽  
M.S. Hossain
Keyword(s):  
Key Words ◽  
Shoot Regeneration ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Momordica Charantia ◽  
Shoot Length ◽  
Bitter Gourd ◽  
Nodal Segments ◽  
Root Segments

Explants of nodal and root segments of bitter gourd were cultured on MS supplemented with various concentrations of BAP in combination with either 2,4-D or NAA. Nodal segments produced the highest percentage (93.75) of callus in MS supplemented with 1.0 mg/l 2,4-D and 1.0 mg/l BAP whereas, root segments produced the highest (85.00%) callus in 0.6 mg/l NAA and 2.5 mg/l BAP combination. A combination of 1.0 mg/l 2,4-D and 1.0 mg/l BAP exhibited 75.00% shoot regeneration from nodal segments. The highest shoot length (5.15 cm) was recorded with 2.5 mg/l BAP and 0.2 mg/l IAA from nodal segments. No sign of regeneration of shoot was found from root segments in any of the combinations. However, some combinations produced only roots.  Key words: In vitro propagation, bitter gourd, nodal segments, Root segments D.O.I. 10.3329/ptcb.v19i1.4916 Plant Tissue Cult. & Biotech. 19(1): 45-52, 2009 (June)

scholarly journals In vitro Propagation of Ionidium suffruticosum Ging. ? A Seasonal Multipotent Medicinal Herb.

1970 ◽  
Vol 19 (2) ◽  
pp. 143-150
Author(s):  
Arunkumar B. Sonappanavar ◽  
M. Jayaraj ◽  
Asha N. Bagadekar ◽  
Anant V. Bhandarkar
Keyword(s):  
Key Words ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Leaf Explants ◽  
Callus Cultures ◽  
Medicinal Herb ◽  
In Vitro Shoots

Indirect regeneration of plant was obtained through organogenesis in leaf callus cultures of Ionidium suffruticosum. Leaf explants were found to be best suited for callus induction on MS with 2, 4-D (0.5 and 1.0 mg/l). Maximum shoot regeneration was obtained in MS supplemented with Kn (4.0 mg/l) alone and NAA (0.4 mg/l) with Kn (2.0 m/l).  The in vitro shoots thus obtained were successfully rooted in MS supplemented with Kn (4.0 mg/l) alone and with NAA (2.0 mg/l) and Kn (0.2  mg/l).  Seventy per cent of the rooted plants survived and they were successfully acclimated in soil. Key words: Ionidium suffruticosum, micropropagation, Medicinal herb D.O.I. 10.3329/ptcb.v19i2.5431 Plant Tissue Cult. & Biotech. 19(2): 143-150, 2009 (December)

scholarly journals In vitro Propagation of Holarrhena antidysenterica Wall., Wedelia chinensis (Osb.) Merr. and Woodfordia fruticosa (L.) Kurz.

1970 ◽  
Vol 19 (2) ◽  
pp. 253-255 ◽  
Author(s):  
S.A.M. Nurul Islam ◽  
Haradhan Banik ◽  
Saiful Alam M. Tarek ◽  
Mahbubur Rahman
Keyword(s):  
Medicinal Plant ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Short Communication ◽  
Multiple Shoot ◽  
Holarrhena Antidysenterica ◽  
Woodfordia Fruticosa

 Key words:  Medicinal plant, Holarrhena antidysenterica, Wedelia chinensis,  Woodfordia fruticosa, In vitro cloning, Multiple shoot D.O.I. 10.3329/ptcb.v19i2.5443 Plant Tissue Cult. & Biotech. 19(2): 253-255, 2009 (December)    - Short communication

scholarly journals In vitro Propagation of Mortiño (Vaccinium floribundum Kunth.)

2020 ◽  
Vol 30 (2) ◽  
pp. 167-177
Author(s):  
Susana Llivisaca ◽  
Juan Cevallos Cevallos ◽  
Joffre Mendoza ◽  
Fernando Piña ◽  
Esther Peralta ◽  
...  
Keyword(s):  
Factorial Design ◽  
Root System ◽  
Plant Species ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Wild Plant ◽  
Multiplication Factor ◽  
Axillary Shoots ◽  
Wild Plant Species

The disinfected axillary shoots of mortino - a wild plant species were cultured in MS with different concentrations (0.002, 0.05 and 0.1%) of methyl N-(1H-benzimidazol-2-yl) carbamate to develop an in vitro protocol for micropropagation through organogenesis. They were then placed in a concentration matrix (0.1, 1.0 and 10.0 μM) whose factorial design involved BAP and IAA in WPM. Results showed a 100% disinfection at 0.05% concentrations of carbendazim and the combination of IAA and BAP at 10 μM yielded a multiplication factor of 3.35, with rooting after seven weeks. This system showed efficient disinfection, multiplication and yielding an acceptable root system. Plant Tissue Cult. & Biotech. 30(2): 167-177, 2020 (December)

scholarly journals Micropropagation of Bacopa monnieri using Cyanobacterial Liquid Medium

1970 ◽  
Vol 20 (2) ◽  
pp. 225-231 ◽  
Author(s):  
Meenakshi Banerjee ◽  
Priyanka Modi
Keyword(s):  
Plant Tissue ◽  
In Vitro Propagation ◽  
Liquid Culture ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Axillary Node ◽  
Bacopa Monnieri ◽  
Shoot Initiation ◽  
Aulosira Fertilissima

Hot extract of Aulosira fertilissima (cyanobacterium) added in different proportions to MS as a liquid culture media for the in vitro propagation of Bacopa monnieri (L.) Pennell. Maximum numbers of shoots were induced from axillary node in MS media (40 ml) + Aulosira extract (60 ml) and maximum shoot multiplication was observed when Kn (1.0 mg/l) was added in the shoot initiation media (mentioned above). Surprisingly rooting was also found to be best in the same combination of MS + cyanobacterial extract that was used for initiation and multiplication of shoots. On an average within a period of three subcultures (2 - 3 months) the nodal explants generated 400 shoots.  Rooted plantlets were successfully transferred to the field, after acclimation in the net house.   Key words: Baccopa monnieri, Cyanobacterial extract, Regeneration, Acclimation   D.O.I. 10.3329/ptcb.v20i2.6917   Plant Tissue Cult. & Biotech. 20(2): 225-231, 2010 (December)

scholarly journals In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots

2011 ◽  
Vol 71 (3) ◽  
pp. 376-382 ◽  
Author(s):  
Rolando García-Gonzáles ◽  
Miladys Delgado ◽  
Yailín González ◽  
Aníbal González ◽  
Miguel Garriga ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Cedrela Odorata
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