Species-specific diagnostic assays for Bonamia ostreae and B. exitiosa in European flat oyster Ostrea edulis: conventional, real-time and multiplex PCR

2013 ◽  
Vol 104 (2) ◽  
pp. 149-161 ◽  
Author(s):  
A Ramilo ◽  
JI Navas ◽  
A Villalba ◽  
E Abollo
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Ostrea Edulis ◽  
Diagnostic Assays ◽  
Bonamia Ostreae ◽  
Flat Oyster ◽  
Species Specific

scholarly journals A non-lethal method for detection of Bonamia ostreae in flat oyster (Ostrea edulis) using environmental DNA

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Louise von Gersdorff Jørgensen ◽  
Johan Wedel Nielsen ◽  
Mikkel Kehler Villadsen ◽  
Bent Vismann ◽  
Sussie Dalvin ◽  
...  
Keyword(s):  
Water Samples ◽  
Sea Water ◽  
Diagnostic Technique ◽  
Environmental Dna ◽  
Detection Methods ◽  
Ostrea Edulis ◽  
Breeding Programs ◽  
Bonamia Ostreae ◽  
Flat Oyster ◽  
Selection Of

Abstract Surveillance and diagnosis of parasitic Bonamia ostreae infections in flat oysters (Ostrea edulis) are prerequisites for protection and management of wild populations. In addition, reliable and non-lethal detection methods are required for selection of healthy brood oysters in aquaculture productions. Here we present a non-lethal diagnostic technique based on environmental DNA (eDNA) from water samples and demonstrate applications in laboratory trials. Forty oysters originating from Limfjorden, Denmark were kept in 30 ppt sea water in individual tanks. Water was sampled 6 days later, after which all oysters were euthanized and examined for infection, applying PCR. Four oysters (10%) were found to be infected with B. ostreae in gill and mantle tissue. eDNA purified from the water surrounding these oysters contained parasite DNA. A subsequent sampling from the field encompassed 20 oysters and 15 water samples from 5 different locations. Only one oyster turned out positive and all water samples proved negative for B. ostreae eDNA. With this new method B. ostreae may be detected by only sampling water from the environment of isolated oysters or isolated oyster populations. This non-lethal diagnostic eDNA method could have potential for future surveys and oyster breeding programs aiming at producing disease-free oysters.

Histopathology and a real-time PCR assay for detection of Bonamia ostreae in Ostrea edulis cultured in western Canada

Aquaculture ◽  
2006 ◽  
Vol 261 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Gary D. Marty ◽  
Susan M. Bower ◽  
Kyle R. Clarke ◽  
Gary Meyer ◽  
Geoff Lowe ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Western Canada ◽  
Ostrea Edulis ◽  
Pcr Assay ◽  
Bonamia Ostreae

Interlaboratory variability in screening for Bonamia ostreae, a protistan parasite of the European flat oyster Ostrea edulis

2014 ◽  
Vol 110 (1) ◽  
pp. 93-99 ◽  
Author(s):  
G Flannery ◽  
SA Lynch ◽  
M Longshaw ◽  
D Stone ◽  
P Martin ◽  
...  
Keyword(s):  
Ostrea Edulis ◽  
Interlaboratory Variability ◽  
Bonamia Ostreae ◽  
Flat Oyster

scholarly journals Characterization of actin genes in Bonamia ostreae and their application to phylogeny of the Haplosporidia

Parasitology ◽  
2007 ◽  
Vol 134 (14) ◽  
pp. 1941-1948 ◽  
Author(s):  
I. LÓPEZ-FLORES ◽  
V. N. SUÁREZ-SANTIAGO ◽  
D. LONGET ◽  
D. SAULNIER ◽  
B. CHOLLET ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Protozoan Parasite ◽  
Ostrea Edulis ◽  
Coding Region ◽  
Actin Genes ◽  
Bonamia Ostreae ◽  
Flat Oyster ◽  
Primer Sets ◽  
Systemic Infections

SUMMARYBonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and demonstrated that the paralogous actin genes found in Bonamia resulted from a duplication of the original actin gene after the Bonamia origin.

scholarly journals Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae

2017 ◽  
Vol 7 ◽  
Author(s):  
Benjamin Morga ◽  
Tristan Renault ◽  
Nicole Faury ◽  
Sophie Lerond ◽  
Céline Garcia ◽  
...  
Keyword(s):  
Ostrea Edulis ◽  
Bonamia Ostreae ◽  
Flat Oyster
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