Diagnosis of helicobacter pylori infection: problems and prospects

2020 ◽  
pp. 54-59
Author(s):  
A. S. Molostova ◽  
N. S. Gladyshev ◽  
A. V. Svarval ◽  
R. S. Ferman ◽  
A. B. Karasyova ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Urease Activity ◽  
Diagnostic Methods ◽  
Study Group ◽  
Biochemical Method ◽  
Antimicrobial Drugs ◽  
Non Invasive ◽  
Number Of Patients ◽  
Pcr Method ◽  
Positive Results

(HP) infection was performed using invasive and non-invasive methods. The study group consisted of 95 patients with dyspepsia. HP infection was detected in 47 patients (49.4 %). The expediency of using a set of diagnostic methods for detecting HP (PCR, immunochromatographic, bacteriological and method for determining urease activity) is proved. Most often (100 %) in patients HP infection was detected in biopsies using the PCR method. Somewhat less frequently it was detected when examining biopsies with an invasive biochemical method (AMA RUT Reader) (82 %) and fecal immunochromatographic method (83 %). Despite the fact that helicobacteriosis was detected bacteriologically in a small number of patients (24 %), this method is of particular value, since it allows you to assess the sensitivity to antimicrobial drugs and probiotics, and does not give false positive results.

scholarly journals Non-invasive diagnostic methods of the liver fibrosis in patients with overweigh and obesity

2021 ◽  
Vol 11 (3) ◽  
pp. 239-245
Author(s):  
Nadiia Gavryliuk ◽  
Ihor Hospodarskyy ◽  
Oksana Prokopchuk ◽  
Olga Namisniak ◽  
Mykhailo Havrylyuk
Keyword(s):  
Liver Fibrosis ◽  
Wide Distribution ◽  
Modern Medicine ◽  
Overweight And Obesity ◽  
Hcv Infection ◽  
Diagnostic Methods ◽  
Non Invasive ◽  
Number Of Patients ◽  
High Prevalence ◽  
Rate Of Progression

One of the biggest problems of modern medicine is the difficulty in early diagnostic and effective treatment of HCV infection due to its wide distribution, latent form and terrible consequences as a formation of liver cirrhosis (CP) and hepatocellular carcinoma (HCC). It is important to mention, that the rate of progression of liver fibrosis. It is an important medical and social problem that the number of patients with NAFLD, overweight and obesity is constantly increases [9]. Due to the high prevalence of NAFLD and CHC, these two pathologies will progress and contribute to the progression of fibrosis, and depending on the genome of the virus and metabolic syndrome. There is a high risk of developing CP and HCC even after achieving a stable virological response.The main aim of our investigation was to assess the stages of fibrosis by using non-invasive methods for patients with overweight and obesity who being treated for HCV infection (with posthepatic fibrosis).

An evaluation of the usefulness of invasive and non-invasive methods used to diagnose Helicobacter spp. infections in dogs

2017 ◽  
Vol 20 (3) ◽  
pp. 491-499 ◽  
Author(s):  
M. Jankowski ◽  
J. Spużak ◽  
K. Kubiak ◽  
K. Glińska-Suchocka ◽  
M. Biernat
Keyword(s):  
Nested Pcr ◽  
Gastric Biopsy ◽  
Diagnostic Methods ◽  
Rapid Urease Test ◽  
Pcr Assay ◽  
Non Invasive ◽  
Biopsy Specimens ◽  
Urease Test ◽  
Invasive Method ◽  
Pcr Method

AbstractThe aim of this study was to assess the suitability of invasive and non-invasive methods used to diagnose Helicobacter spp. in the stomachs of dogs. The study was carried out on 30 dogs of both sexes and different breeds, between one and 15 years old. A histopathologic examination, a microbiological culture, a rapid urease test, a direct bacteriological preparation and a nested PCR assay were carried out. Gastric Helicobacter spp. was identified in gastric biopsy specimens from 16 (53.3%) dogs using direct bacteriological preparation, in four (13.3%) dogs based on a culture, in 23 (76.6%) dogs using the rapid urease test and in 21 (70,0%) dogs based on a histopathological assessment of the biopsy specimens. The nested PCR of the gastric biopsy specimens revealed gastric Helicobacter spp. in all the dogs (100%). A saliva PCR assay revealed gastric Helicobacter spp. in 23 (76.6%) dogs, while stool PCR revealed the bacterium in seven (23.3%) dogs. We found that invasive methods were more accurate than non-invasive methods in detecting a Helicobacter spp. infection in dogs. In addition, the nested PCR method used to evaluate the gastric mucosal biopsy specimens was the most accurate test for detecting Helicobacter spp. It was further found that the PCR-based saliva assay was the best non-invasive method for detecting Helicobacter spp. However, taking into consideration that most of the diagnostic methods used to detect this bacterium have drawbacks, at least two diagnostic methods should be used to detect Helicobacter spp. as is done in human medicine.

scholarly journals Diagnosis of Helicobacter pylori infection in clinical practice

2018 ◽  
Vol 90 (12) ◽  
pp. 133-139 ◽  
Author(s):  
D S Bordin ◽  
I N Voynovan ◽  
S V Kolbasnikov ◽  
Yu V Embutnieks
Keyword(s):  
Helicobacter Pylori ◽  
Clinical Practice ◽  
Diagnostic Tests ◽  
Urease Activity ◽  
Molecular Detection ◽  
Clinical Situation ◽  
Helicobacter Pylori Infection ◽  
Indirect Detection ◽  
Antisecretory Drugs ◽  
Non Invasive

Diagnostic tests used to detection Helicobacter pylori are divided into invasive (requiring endoscopy) and non-invasive, direct (bacteriological, histological or molecular detection of the bacterium) and indirect (detection of urease activity of the bacterium or antibodies to it). The choice of the test is determined by the clinical situation, as well as by its availability and cost. The sensitivity of most tests is affected by the use of antisecretory drugs and antibiotics.

scholarly journals Current Helicobacter pylori Diagnostics

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1458
Author(s):  
Dmitry S. Bordin ◽  
Irina N. Voynovan ◽  
Dmitrii N. Andreev ◽  
Igor V. Maev
Keyword(s):  
Helicobacter Pylori ◽  
Diagnostic Tests ◽  
Precancerous Lesions ◽  
Primary Diagnosis ◽  
Blue Laser ◽  
Diagnostic Methods ◽  
Stool Antigen Test ◽  
Advantages And Disadvantages ◽  
Non Invasive ◽  
H Pylori

The high prevalence of Helicobacter pylori and the variety of gastroduodenal diseases caused by this pathogen necessitate the use of only accurate methods both for the primary diagnosis and for monitoring the eradication effectiveness. There is a broad spectrum of diagnostic methods available for detecting H. pylori. All methods can be classified as invasive or non-invasive. The need for upper endoscopy, different clinical circumstances, sensitivity and specificity, and accessibility defines the method chosen. This article reviews the advantages and disadvantages of the current options and novel developments in diagnostic tests for H. pylori detection. The progress in endoscopic modalities has made it possible not only to diagnose precancerous lesions and early gastric cancer but also to predict H. pylori infection in real time. The contribution of novel endoscopic evaluation technologies in the diagnosis of H. pylori such as visual endoscopy using blue laser imaging (BLI), linked color imaging (LCI), and magnifying endoscopy is discussed. Recent studies have demonstrated the capability of artificial intelligence to predict H. pylori status based on endoscopic images. Non-invasive diagnostic tests such as the urea breathing test and stool antigen test are recommended for primary diagnosis of H. pylori infection. Serology can be used for initial screening and epidemiological studies. The histology showed its value in detecting H. pylori and provided more information about the degree of gastric mucosa inflammation and precancerous lesions. Molecular methods are mainly used in detecting antibiotic resistance of H. pylori. Cultures from gastric biopsies are the gold standard and recommended for antibiotic susceptibility tests.

Invasive and non-invasive Helicobacter pylori diagnostic methods in Iran

Gene Reports ◽  
2020 ◽  
Vol 20 ◽  
pp. 100749
Author(s):  
Samin Alihosseini ◽  
Mehran Jaberinezhad ◽  
Fatemah SadeghpourHeravi ◽  
Reza Ghotaslou ◽  
Hamed Ebrahimzadeh Leylabadlo
Keyword(s):  
Helicobacter Pylori ◽  
Diagnostic Methods ◽  
Non Invasive

scholarly journals CHALLENGES OF DIAGNOSIS OF OBSTRUCTIVE CORONARY ARTERY DISEASE: THE ROLE OF NON-INVASIVE TESTING

2019 ◽  
Vol 8 (1) ◽  
pp. 70-79 ◽  
Author(s):  
E. V. Korok ◽  
A. N. Sumin
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Stable Coronary Artery Disease ◽  
Obstructive Coronary Artery Disease ◽  
Diagnostic Methods ◽  
National Guidelines ◽  
Diagnostic Strategies ◽  
Non Invasive ◽  
Number Of Patients ◽  
Artery Disease

High mortality from cardiovascular diseases, especially coronary artery disease (CAD), represents a major economic burden on healthcare systems. The number of diagnostic coronary angiographies (CAG) is increasing in the Russian Federation. However, routine invasive CAG is not able to determine obstructive coronary artery disease in a considerable number of patients. The rate of intact coronary arteries reaches 42% in the major registries and accounts for 37.9% in our Research Institute. Despite all improvements in the diagnostic algorithms focused on suspected coronary artery disease, stated in the recent international and national guidelines, and the availability of advanced non-invasive diagnostic methods, they do not improve accurate CAG diagnosis of obstructive coronary artery disease. Moreover, the available guidelines contain many contradictions. Therefore, an optimal diagnostic algorithm of significant coronary artery lesions is a subject of discussion. The proposed review discusses all discrepancies concerning the assessment of the pretest probability as well as the proposed non-invasive testing. In addition, the preferred use of non-invasive tests with anatomical assessment (i.e. multispiral computed tomography coronary imaging) rather than functional tests with visualization is stated. This review ensures better understanding of diagnostic strategies for stable coronary artery disease used both in researches and in clinical practice.

scholarly journals Evaluation of invasive and non-invasive methods for the diagnosis of Helicobacter pylori infection in symptomatic children and adolescents

2001 ◽  
Vol 119 (2) ◽  
pp. 67-71 ◽  
Author(s):  
Silvio Kazuo Ogata ◽  
Elisabete Kawakami ◽  
Francy Reis Silva Patrício ◽  
Margareth Zabeu Pedroso ◽  
Antonio Mario Santos
Keyword(s):  
Helicobacter Pylori ◽  
Children And Adolescents ◽  
Breath Test ◽  
Urea Breath Test ◽  
Diagnostic Methods ◽  
Helicobacter Pylori Infection ◽  
Rapid Urease Test ◽  
Predictive Values ◽  
Non Invasive ◽  
Urease Test

CONTEXT: Multiple diagnostic methods are available for the detection of Helicobacter pylori infection, but at present no single one can be used as the gold standard. OBJECTIVE: The aim of this study was to evaluate the diagnostic accuracy of 3 invasive and 2 non-invasive methods for detection of Helicobacter pylori infection in symptomatic children and adolescents. DESIGN: Prospective cohort study SETTING: Peptic Disease outpatients service, Discipline of Pediatric Gastroenterology, Universidade Federal de São Paulo / Escola Paulista de Medicina. PATIENTS: Forty-seven patients who underwent endoscopy because of dyspeptic symptoms. DIAGNOSTIC METHODS: Endoscopy with gastric biopsies for 3 invasive (rapid urease test, histology and culture) and 2 non-invasive methods (a commercial ELISA serology and 13carbon urea breath test - isotope ratio mass spectrometry) for detection of Helicobacter pylori infection. MAIN MEASUREMENTS: Sensitivity, specificity, positive and negative predictive values of each method and agreement and disagreement rates between the methods. RESULTS: Forty-seven patients [mean age, 11y9mo (SD 2y10mo), 27 female and 20 male]; 62% of them were Helicobacter pylori-positive. All methods agreed in 61%, and were negative in 21% and positive in 40%. The greatest concordance between 2 methods occurred between the invasive methods: histology and rapid urease test (89.6%) and histology and culture (87.5%). The greatest sensitivity, considering Helicobacter pylori-positive cases, for any combination of 3 or more tests, was achieved by the rapid urease test (S=100%), followed by histology, serology and 13carbon-urea breath test (S=93.1%) and lastly by culture (S=79.3%). The highest specificity was obtained by histology (100%) and culture (100%), followed by the rapid urease test (84.2%), serology (78.9%) and 13carbon-urea breath test (78.9%). CONCLUSIONS: Our results suggest that among invasive methods, an association between the rapid urease test and histology constituted the best choice for the detection of Helicobacter pylori infection. If results of histology and the rapid urease test are different, serology may be recommended.

The influence of CAG a positivity on the accuracy of non invasive diagnostic methods and eradication rate in Helicobacter pylori infection

2003 ◽  
Vol 124 (4) ◽  
pp. A179
Author(s):  
Istvan Racz ◽  
Emese Babarczy ◽  
Andrea Szabo ◽  
Gyula Pecsi ◽  
Mihaly Csondes ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Eradication Rate ◽  
Diagnostic Methods ◽  
Helicobacter Pylori Infection ◽  
Non Invasive ◽  
Cag A

Conflicting results of non-invasive methods for detection of Helicobacter pylori infection in children with celiac disease - a preliminary study.

2016 ◽  
Vol 63 (1) ◽  
Author(s):  
Jan Józefczuk ◽  
Edyta Mądry ◽  
Jan Nowak ◽  
Marek Walkowiak ◽  
Klaudia Łochocka ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Celiac Disease ◽  
Healthy Subjects ◽  
Breath Test ◽  
Urea Breath Test ◽  
Antigen Test ◽  
Non Invasive ◽  
Preliminary Study ◽  
Level Of Significance ◽  
Positive Results

There are no data addressing the usefulness of non-invasive tests for the detection of Helicobacter pylori (HP) infection in celiac disease (CD). The aim of this study was to compare two most sensitive and specific tests - urea breath test (UBT) and fecal antigen test (FAT) in HP diagnosis in CD patients. The study comprised of 76 CD patients, 49 healthy subjects (HS) and 35 patients who underwent differential diagnosis due to abdominal pain (AP patients). The presence of HP infection was evaluated using the (13)C isotope-labeled UBT and FAT (ELISA). HP infection was diagnosed based on UBT and FAT in 8 (16.3%) and 7 (14.3%) HS, and in 8 (10.5%) CD patients and 12 (34.3%) AP patients, respectively, using both tests. The prevalence of conflicting results in comparison with positive results (obtained with any of the two tests) was distinctly higher (54.5%) in CD group than in other subjects (23.3%); however, due to low HP prevalence, it did not reach the level of significance (p

The comparision of two diagnostic methods for detection of Helicobacter pylori in gastric mucosa

1997 ◽  
Vol 78 (5) ◽  
pp. 339-340
Author(s):  
V. V. Krasin ◽  
R. Sh. Chasanov ◽  
R. I. Garifullin ◽  
P. A. Maksimov ◽  
A. P. Kiassov
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Urease Activity ◽  
Polyclonal Antibodies ◽  
Diagnostic Methods ◽  
Immunocytochemical Staining ◽  
Immunocytochemical Method

The two diagnostic methods for detection of Helicobacter pylori have been tested. The first one was de-nol test, which was based on detection of the urease activity. The second one was the immunocytochemical method using the polyclonal antibodies against Helicobacter pylori. During the fibrogastroscopy of patients with gastric desease (20 persons) the small pieces of gastric mucosa were taken. The latters were used for preparation of smears and then for de-nol test. 18 patients have got the positive de-nol tests, and the analyses of two patients were negative. However, the Helicobacter pylori were detected in all cases, using the immunocytochemical staining with polyclonal antibodies against this bacterium. On the base of these results we have suggested that immunocytochemical staining of Helicobacter pylori is more informative and more precise than de-nol test.

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