scholarly journals Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments

2021 ◽  
Vol 9 ◽  
Author(s):  
Yu Pan ◽  
Zhao Liu ◽  
Yijie Wang ◽  
Linshen Zhang ◽  
Niying Chua ◽  
...  
Keyword(s):  
Cellular Proliferation ◽  
Skin Inflammation ◽  
Medical Regimen ◽  
Anti Cancer ◽  
Colorectal Cancer Cells ◽  
Inflammatory Bowel ◽  
Therapeutic Properties ◽  
Anti Inflammatory ◽  
Oxidative Effects

Chronic inflammation is considered a pressing health issue that needs resolving. Inflammatory disease such as inflammatory bowel disease requires a long-term medical regimen to prevent disease progression. Conventionally, lactoferrin is used to treat mild gastrointestinal tract and skin inflammation. Protease-digested lactoferrin fragments often exhibit improved therapeutic properties compared to full-length lactoferrin (flHLF). However, there are no studies on the use of protease-digested lactoferrin fragments to treat inflammation. Herein, we assess the anti-inflammatory properties of engineered recombinant lactoferrin fragments (rtHLF4, rteHLF1, and rpHLF2) on non-malignant colonic fibroblast cells and colorectal cancer cells. We found that rtHLF4 is 10 times more effective to prevent inflammation compared to flHLF. These results were investigated by looking into the reactive oxygen species (ROS) production, angiogenesis activity, and cellular proliferation of the treated cells. We have demonstrated in this study the anti-inflammatory properties of the flHLF and the various lactoferrin fragments. These results complement the anti-cancer properties of these proteins that were demonstrated in an earlier study.

Effects of probiotic bacteria on mucosal polyamines levels in dogs with IBD and colonic polyps: a preliminary study

2018 ◽  
Vol 9 (2) ◽  
pp. 247-255 ◽  
Author(s):  
G. Rossi ◽  
M. Cerquetella ◽  
S. Scarpona ◽  
G. Pengo ◽  
K. Fettucciari ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Caspase 3 ◽  
Cellular Proliferation ◽  
Activity Index ◽  
Clinical Activity ◽  
High Concentration ◽  
Ki 67 ◽  
Inflammatory Bowel ◽  
Anti Inflammatory

Spermine (SPM) and its precursor putrescine (PUT), regulated by ornithine decarboxylase (ODC) and diamino-oxidase (DAO), are polyamines required for cell growth and proliferation. Only a few studies have investigated the anti-inflammatory and tumour inhibitory properties of probiotics on mucosal polyamine levels. We investigated the effects of a high concentration multistrain probiotic for human use on colonic polyamine biosynthesis in dogs. Histological sections (inflammatory bowel disease, n=10; polyposis, n=5) were assessed after receiving 112 to 225×109 lyophilised bacteria daily for 60 days at baseline (T0) and 30 days after treatment end (T90). Histology scores, expression of PUT, SPM, ODC and DAO, and a clinical activity index (CIBDAI) were compared at T0 and T90. In polyps, cellular proliferation (Ki-67 expression), and apoptosis (caspase-3 protein expression) were also evaluated. After treatment, in inflammatory bowel disease significant decreases were observed for CIBDAI (P=0.006) and histology scores (P<0.001); PUT, SPM and ODC expression increased (P<0.01). In polyps, a significant decrease in polyamine levels, ODC activity, and Ki-67, and a significant increase in caspase-3 positivity and DAO expression (P=0.005) was noted. Our results suggest potential anti-proliferative and anti-inflammatory effects of the probiotic mixture in polyps and inflammation, associated with reduced mucosal infiltration and up-regulation of PUT, SPM, and ODC levels.

Dynamics of telomeric chromatin at the crossroads of aging and cancer

2010 ◽  
Vol 48 ◽  
pp. 147-164 ◽  
Author(s):  
Jing Ye ◽  
Yunlin Wu ◽  
Eric Gilson
Keyword(s):  
Chromosomal Instability ◽  
Cellular Proliferation ◽  
Human Cancer ◽  
Chromosome Instability ◽  
Telomere Dysfunction ◽  
Promising Candidate ◽  
Telomere Shortening ◽  
Anti Cancer ◽  
Protein Components

Telomeres are nucleoprotein structures that protect the ends of human chromosomes through the formation of a ‘cap’, thus preventing exonucleolytic degradation, inter- and intra-chromosomal fusion, and subsequent chromosomal instability. During aging, telomere shortening correlates with tissue dysfunction and loss of renewal capacity. In human cancer, telomere dysfunction is involved in early chromosome instability, long-term cellular proliferation, and possibly other processes related to cell survival and microenvironment. Telomeres constitute an attractive target for the development of novel small-molecule anti-cancer drugs. In particular, individual protein components of the core telomere higher-order chromatin structure (known as the telosome or ‘shelterin’ complex) are promising candidate targets for cancer therapy.

scholarly journals The Use of Aspirin for the Treatment of Malignancies: Review Study

2016 ◽  
Vol 12 (36) ◽  
pp. 430
Author(s):  
Dana Ayman Abdel Ra’ouf Alnsour ◽  
Ahed J Alkhatib
Keyword(s):  
Colorectal Cancer ◽  
Cancer Risk ◽  
Inflammatory Mediators ◽  
Epidemiological Studies ◽  
The Other ◽  
Antineoplastic Effect ◽  
Therapeutic Properties ◽  
Review Study ◽  
Anti Inflammatory

The present review study reviewed the literature for the possible therapeutic roles of aspirin against tumors. Aspirin has various therapeutic properties among which is the anti-inflammatory property. Epidemiological studies have proved the efficacy of long term use of aspirin in reducing the incidence of colorectal cancer by up to 30%, and the cancer risk as well. Although no exact mechanism has been entirely proposed so far to explain the antineoplastic effect of aspirin against cancer, two mechanisms have been under focus, the first is related to its anti-inflammatory properties which are thought to suppress the inflammatory mediators and to inhibit carcinogenic mechanisms, and the other mechanism is through the deactivation of COX2. We would like to raise the possibility for aspirin to be regularly involved in preventive mechanisms.

scholarly journals Development of NOSH-NSAIDs: a new class of anti-inflammatory pharmaceuticals for the treatment of cancer

2017 ◽  
Vol 39 (4) ◽  
pp. 24-29
Author(s):  
Khosrow Kashfi
Keyword(s):  
Human Cancer ◽  
Tumour Mass ◽  
Human Cancer Cell Lines ◽  
New Class ◽  
Cycle Arrest ◽  
Anti Cancer ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Better Than

Non-steroidal anti-inflammatory drugs (NSAIDs) have anti-cancer (chemopreventive) properties; however, side effects preclude their long-term use. NOSH-NSAIDs, designed as safer alternatives, are novel hybrid chimaeras that release nitric oxide (NO) and hydrogen sulfide (H2S). NOSH-NSAIDs are gastrointestinally safe yet retain all the pharmacological properties of their native NSAID. NOSHNSAIDs are orders of magnitude more potent than their conventional counterparts in inhibiting the growth of various human cancer cell lines of different tissue origins, adenomatous, epithelial and lymphocytic. This growth inhibition is a result of a reduction in cell proliferation and cell cycle arrest, leading to increased apoptosis. In xenograft mouse models of cancer, NOSH-aspirin was better than normal aspirin as a chemopreventive agent; it dose-dependently inhibited tumour growth and tumour mass. NOSH-naproxen was significantly more efficacious than normal naproxen in reducing the growth of established tumours.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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