scholarly journals Yeasts as Biopharmaceutical Production Platforms

2021 ◽  
Vol 2 ◽  
Author(s):  
Natalja Kulagina ◽  
Sébastien Besseau ◽  
Charlotte Godon ◽  
Gustavo H. Goldman ◽  
Nicolas Papon ◽  
...  
Keyword(s):  
Biopharmaceutical Production

The transgenic animal platform for biopharmaceutical production

2016 ◽  
Vol 25 (3) ◽  
pp. 329-343 ◽  
Author(s):  
L. R. Bertolini ◽  
H. Meade ◽  
C. R. Lazzarotto ◽  
L. T. Martins ◽  
K. C. Tavares ◽  
...  
Keyword(s):  
Transgenic Animal ◽  
Biopharmaceutical Production

scholarly journals Enzyme-free release of adhered cells from standard culture dishes using intermittent ultrasonic traveling waves

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Yuta Kurashina ◽  
Chikahiro Imashiro ◽  
Makoto Hirano ◽  
Taiki Kuribara ◽  
Kiichiro Totani ◽  
...  
Keyword(s):  
Traveling Wave ◽  
Acoustic Pressure ◽  
Cultured Cells ◽  
Cell Damage ◽  
Free Cell ◽  
Cell Detachment ◽  
Adherent Cells ◽  
Standard Culture ◽  
Biopharmaceutical Production ◽  
Efficient Transfer

Abstract Cell detachment is essential in culturing adherent cells. Trypsinization is the most popular detachment technique, even though it reduces viability due to the damage to the membrane and extracellular matrix. Avoiding such damage would improve cell culture efficiency. Here we propose an enzyme-free cell detachment method that employs the acoustic pressure, sloshing in serum-free medium from intermittent traveling wave. This method detaches 96.2% of the cells, and increases its transfer yield to 130% of conventional methods for 48 h, compared to the number of cells detached by trypsinization. We show the elimination of trypsinization reduces cell damage, improving the survival of the detached cells. Acoustic pressure applied to the cells and media sloshing from the intermittent traveling wave were identified as the most important factors leading to cell detachment. This proposed method will improve biopharmaceutical production by expediting the amplification of tissue-cultured cells through a more efficient transfer process.

scholarly journals Exploring the potential of biopharmaceutical production by Rigidoporus ulmarius: Cultivation, chemistry, and bioactivity studies

2009 ◽  
Vol 44 (11) ◽  
pp. 1237-1244 ◽  
Author(s):  
Jing-Jy Cheng ◽  
Huu-Sheng Lur ◽  
Nai-Kuei Huang ◽  
Hsuan-Pei Chen ◽  
Cha-Yui Lin ◽  
...  
Keyword(s):  
Biopharmaceutical Production

Multiplex RT Q-PCR assay for simultaneous quantification of three viruses used for validation of virus clearance by biopharmaceutical production

Biologicals ◽  
2009 ◽  
Vol 37 (5) ◽  
pp. 331-337 ◽  
Author(s):  
Scott Lute ◽  
Hua Wang ◽  
Davonie Sanchez ◽  
Janet Barletta ◽  
Qi Chen ◽  
...  
Keyword(s):  
Pcr Assay ◽  
Simultaneous Quantification ◽  
Virus Clearance ◽  
Biopharmaceutical Production

scholarly journals The cryo-EM structure of vesivirus 2117 highlights functional variations in entry pathways for viruses in different clades of the Vesivirus genus

2021 ◽  
Author(s):  
Hazel Sutherland ◽  
Michaela J. Conley ◽  
Edward Emmott ◽  
James Streetley ◽  
Ian G. Goodfellow ◽  
...  
Keyword(s):  
Major Capsid Protein ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Outer Face ◽  
Receptor Binding Site ◽  
Amino Acid Insertion ◽  
Positive Sense ◽  
Lost Productivity ◽  
Biopharmaceutical Production ◽  
Immunodominant Epitopes

AbstractVesivirus 2117 is an adventitious agent that has been responsible for lost productivity in biopharmaceutical production following contamination of Chinese hamster ovary cell cultures in commercial bioreactors. A member of the Caliciviridae, 2117 is classified within the Vesivirus genus in a clade that includes canine and mink caliciviruses but is distinct from the vesicular exanthema of swine clade, which includes the extensively studied feline calicivirus (FCV). We have used cryogenic electron microscopy (cryo-EM) to determine the structure of the capsid of this small, icosahedral, positive-sense RNA containing virus. We show that the outer face of the dimeric capsomeres, which contains the receptor binding site and major immunodominant epitopes in all caliciviruses studied thus far, is quite different from that of FCV. This is a consequence of a 22 amino-acid insertion in the sequence of the FCV major capsid protein that forms a ‘cantilevered arm’, which plays an important role in both receptor engagement and undergoes structural rearrangements thought to be important for genome delivery to the cytosol. Our data highlight a potentially important difference in the attachment and entry pathways employed by the different clades of the Vesivirus genus.

scholarly journals Process engineering of biopharmaceutical production in moss bioreactors via model-based description and evaluation of phytohormone impact

2021 ◽  
Author(s):  
Natalia Ruiz-Molina ◽  
Juliana Parsons ◽  
Sina Schroeder ◽  
Clemens Posten ◽  
Ralf Reski ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Co2 Enrichment ◽  
Fold Increase ◽  
Continuous Operation ◽  
Specific Productivity ◽  
Fed Batch ◽  
Synthesis Inhibitor ◽  
Auxin Synthesis ◽  
Biopharmaceutical Production ◽  
The Impact

The moss Physcomitrella is an interesting production host for recombinant biopharmaceuticals. Here we produced MFHR1, a synthetic complement regulator which has been proposed for the treatment of diseases associated to the complement system as part of human innate immunity. We studied the impact of different operation modes for the production process in 5 L stirred-tank photobioreactors. The total amount of recombinant protein was doubled by using fed-batch or batch compared to semi-continuous operation, although the maximum specific productivity (mg MFHR1/g FW) increased just by 35%. We proposed an unstructured kinetic model which fits accurately with the experimental data in batch and semi-continuous operation under autotrophic conditions with 2% CO2 enrichment. The model is able to predict recombinant protein production, nitrate uptake and biomass growth, which is useful for process control and optimization. We investigated strategies to further increase MFHR1 production. While mixotrophic and heterotrophic conditions decreased the MFHR1-specific productivity compared to autotrophic conditions, addition of the phytohormone auxin (NAA, 10 μM) to the medium enhanced it by 470% in shaken flasks and up to 230% and 260%, in batch and fed-batch bioreactors, respectively. Supporting this finding, the auxin-synthesis inhibitor L-Kynurenine (100 μM) decreased MFHR1 production significantly by 110% and 580% at day 7 and 18, respectively. Expression analysis revealed that the MFHR1 transgene, driven by the Physcomitrella actin5 (PpAct5) promoter, was upregulated 16 hours after NAA addition and remained enhanced over the whole process, whereas the auxin-responsive gene PpIAA1A was upregulated within the first two hours, indicating that the effect of auxin on PpAct5 promoter-driven expression is indirect. Furthermore, the day of NAA supplementation was crucial, leading to an up to 8-fold increase of MFHR1-specific productivity (0.82 mg MFHR1/ g fresh weight, 150 mg accumulated over 7 days) compared to the productivity reported previously. Our findings are likely to be applicable to other plant-based expression systems to increase biopharmaceutical production and yields.

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