Janette Furuzawa-Carballeda
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Gabriela Fonseca-Camarillo
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Guadalupe Lima
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Jesús K. Yamamoto-Furusho
Aim. To characterise and enumerate IDO+cells, Tregs, and T cell subsets in patients with ulcerative colitis (UC) and Crohn’s disease (CD) with regard to their clinical activity.Methods. Ten active UC (aUC), 10 inactive UC (iUC), 6 aCD, and 8 iCD patients and 10 healthy individuals were included in the study. Circulating Foxp3-, IDO-, IL-17A-, IL-4-, IFN-γ-, and IL-10-expressing CD4+T cells were quantitated by flow cytometry. Interleukin-17-expressing cells, CD25+/Foxp3+Tregs, and CD123+/IDO+plasmacytoid dendritic cells were evaluated in intestinal biopsies from 10 aUC, 6 aCD, and 10 noninflamed tissues.Results. All CD4+T subsets were increased in aIBD patients compared with healthy donors. Meanwhile, frequency of CD8α+/CD16+/IDO+, CD8α+/CD56+/IDO+, CD8α+/CD80+/IDO+, CD8α+/CD123+/IDO+large granular nonlymphoid cells, and CCR6+/CD123+/IDO+plasmacytoid dendritic cells was higher in aIBD patients versus healthy donors or iIBD patients. Tissue IL-17A+cells were present in higher amounts in aIBD versus noninflamed controls. IDO- and Foxp3-expressing cells were increased in aUC versus aCD patients and noninflamed tissues.Conclusions. The findings represent an original work in Mexican Mestizo patients with IBD. It shows that Tregs and IDO-expressing cells are increased with regard to disease activity. These cells could significantly shape inflammatory bowel disease pathophysiology, severity, and tolerance loss.