scholarly journals Comparative Analysis of the Measles Antibody Levels in Healthy Medical Personnel of Maternity Ward and Women in Labor

2021 ◽  
Vol 12 ◽  
Author(s):  
Mikhail Petrovich Kostinov ◽  
Pavel Ivanovich Zhuravlev ◽  
Lylia Solomonovna Gladkova ◽  
Kirill Vadimovich Mashilov ◽  
Valentina Borisovna Polishchuk ◽  
...  
Keyword(s):  
Blood Serum ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Measles Virus ◽  
Age Group ◽  
Igg Antibodies ◽  
Maternity Ward ◽  
Serum Samples ◽  
Ward Personnel

It has been proven that post-vaccination immunity to measles virus after two doses of vaccine is not able to persistently protect against infection throughout life. The goal of this research was to determine the immune layer to the measles virus among women in labor and maternity ward personnel in the same medical institution. The levels of IgG antibodies to measles virus in the umbilical cord blood of 594 women in labor and 88 workers of the maternity ward were studied by ELISA. It was revealed that 22.7% of umbilical cord blood serum samples from parturient women and 21.4% of blood serum samples from maternity ward personnel were seronegative (<0.18 IU/ml). Levels of IgG antibodies to measles virus in low values (<1.0 IU/ml) were detected in 67% of blood serum samples among women in labor and 68.9% among employees of the maternity ward. Among women in labor, women under 35 years of age are at the highest risk of contracting measles; the proportion of women with low levels of protective antibodies in this age group was almost 70%, and the proportion of women without protective levels of antibodies was 23%. Compared with the age group 36–43, the age of women in labor under 35 was associated with a higher chance of not having immune protection against infection with measles virus OR [95% CI] = 2.2 [1.1–4.5] (p = 0.02) or had a low level of protection OR [95% CI] = 1.9 [1.2–3.0] (p = 0.001). It was also found that among women over 35 years of age, the proportion of persons with a high level of antibodies in women in labor was statistically significantly higher than among members of the maternity ward staff (13 and 0%, respectively, p = 0.007). Thus, maternity ward employees and women in labor constitute a risk group for measles due to the presence of a high proportion of seronegative persons among women of childbearing age (both maternity ward employees and women in labor). These conditions create the need to revise current approaches to present vaccination procedures, especially in the current epidemiological situation with COVID-19.

Establishing Relationship Between Maternal Sociodemographic Characteristics and Lead in Umbilical Cord Blood Serum

2018 ◽  
Vol 4 (1) ◽  
pp. 404-410
Author(s):  
Nazia Tarannum ◽  
Ranu Agarwal ◽  
Amit Upadhyay ◽  
Priyanka Gupta ◽  
Rakesh Kumar Soni
Keyword(s):  
Blood Serum ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Lead Concentration ◽  
Serum Samples ◽  
Cord Blood Serum ◽  
Piped Water ◽  
Umbilical Cord Blood Serum ◽  
Epidemiological Characteristics

Aim: To find the correlation between the concentration of lead and maternal socio-demographic characteristics like age, living area (urban or rural), housing style (slum type, cemented, or floored), living place (near industry or far from industry), water supply (piped water or direct water from source) and epidemiological characteristics like mother’s occupation, mother’s gestation age, mother’s active smoking habit. Method: A total of 100 umbilical cord blood serum samples were collected from Lala Lajpat Rai Medical College, Meerut and estimation of lead was done using Graphite Furnace Atomic Absorption Spectroscopy (AAS). This study was conducted between April 2014 and March 2015. Result and conclusions: Data suggested that the percentage of lead is more in cord blood serum samples of mother who are working and are non-housewife and residing in urban area due to exposure to traffic, industries and pollution. The correlation of mother’s working place with lead concentration showed that the mothers who were industrial worker had more lead concentration whereas the level of heavy metal was found elevated in mother’s who are drinking piped water. The data suggested that the mothers who smoked have more lead concentration in comparison to non-smoking mother.

scholarly journals Measles virus-specific IgG-antibodies level in umbilical cord blood according to the maternal age

2014 ◽  
Vol 19 (3) ◽  
pp. 30-34 ◽  
Author(s):  
M. P Kostinov ◽  
A. D Shmitko ◽  
I. I Bocharova ◽  
A. P Cherdancev ◽  
A. A Savisko ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Measles Virus ◽  
Maternal Age ◽  
Adult Population ◽  
Morbidity Rate ◽  
Igg Antibodies ◽  
Protective Antibodies ◽  
High Level

Introduction. Mass vaccination and re-vaccination against measles as of children, as well additionally in adults, contributed to the significant decrease in morbidity rate. At the same time, outbreaks of measles in recent years, both in the World and in Russia, became more frequent, especially in the adult population. Aim: To determine the content of transplacental IgG-antibodies to measles virus in the umbilical cord blood, with taking into account of the age of the mother. Materials and methods. There was studied the cord blood of offsprings of 672 parturient women aged 16-43 years old, residing in Moscow and Moscow region, without a history of measles previously. The level of IgG-antibodies was determined by ELISA with the use of a standard set of reagents of the Firm "VECTOR-BEST", accordingly to instructions. Results. Protective (0.18 IU/ml) IgG-antibodies to measles virus in neonates were recorded in 79.9%. In females aged of 16-20 years protective antibodies were detected in 89,7%, 21-25 years - 80,8%, 26-30 years - 75% 31-35 - 75% 36-43 - 88,2% of cases. Average protective antibody level decreased according to the maternal age, respectively, and was the lowest (1,34 ± 0 22 IU/ml) in the group of women aged of 26 to 30 years and the high level was noted in 16-20 and 36-43 years old cases (2,18 ± 0,47 IU/ml and 2,73 ± 0,44 IU/ml). Conclusion. Women aged of 26-30 years are at risk for the development of measles infection, which is an indication for vaccination before pregnancy.

scholarly journals Human Cord Blood Serum-Derived APP α-Secretase Cleavage Activity is Mediated by C1 Complement

2018 ◽  
Vol 27 (4) ◽  
pp. 666-676 ◽  
Author(s):  
Ahsan Habib ◽  
Darrell Sawmiller ◽  
Huayan Hou ◽  
Manasa Kanithi ◽  
Jun Tian ◽  
...  
Keyword(s):  
Amyloid Precursor Protein ◽  
Blood Serum ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Precursor Protein ◽  
Human Umbilical Cord ◽  
Amyloid Β Peptide ◽  
Cord Blood Serum ◽  
The Impact

Alzheimer’s Disease (AD) is the leading cause of dementia in the elderly. In healthy individuals, amyloid precursor protein (APP) is cleaved by α-secretase, generating soluble α-amyloid precursor protein (sAPPα), which contributes neuroprotective functions in the neuronal environment. In contrast, in the neurodegenerative environment of AD patients, amyloid-β-peptide (Aβ) of either 40 or 42 residues are generated by increased activity of β- and γ-secretase. These proteins amalgamate in specific regions of the brain, which disrupts neuronal functions and leads to cognitive impairment. Human umbilical cord blood cells (HUCBC) have proven useful as potential immunomodulatory therapies in various models of neurodegenerative diseases, including AD. Our most recent work studied the impact of umbilical cord blood serum (CBS) on modulation of sAPPα production. Heat-sensitive CBS significantly promoted sAPPα production, indicating that heat-sensitive factor(s) play(s) a role in this process. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis was used to determine the molecular source of α-secretase in purified CBS and aged blood serum (AgBS) fraction. Of the proteins identified, the subunits of C1 complex (C1q, C1r, and C1s) and alpha-2-macroglobulin showed significantly greater levels in purified α-CBS fraction (α-CBSF) compared with the AgBS fraction (AgBSF). Specifically, C1 markedly increased sAPPα and alpha-carboxyl-terminal fragment (α-CTF) production in a dose-dependent fashion, whereas C1q alone only minimally increased and C3 did not increase sAPPα production in the absence of sera. Furthermore, C1q markedly increased sAPPα and α-CTF, while decreasing Aβ, in CHO/APPwt cells cultured in the presence of whole sera. These results confirm our initial assumption that APP α-secretase activity in human blood serum is mediated by complement C1, opening a potential therapeutic modality for the future of AD.

scholarly journals Blood serum myokine irisin and adipocytokine levels in newborns small for gestational age at birth

2021 ◽  
Vol 18 (4) ◽  
pp. 402-412
Author(s):  
V. A. Prylutskaya ◽  
A. V. Sukalo ◽  
A. V. Goncharik
Keyword(s):  
Blood Serum ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Gestational Age ◽  
Small For Gestational Age ◽  
The Body ◽  
Delivery Mode ◽  
Gestational Age At Birth ◽  
Age At Birth

In recent years, a number of the studies of myokine irisin in adults and isolated in newborns have been carried out. The role of adipocytokines in the growth and development of the fetus and children has been shown.The aim of the study was to assess the levels of myokine irisin and adipocytokines in newborns small for gestational age at birth and to analyze the relationship between the parameters of the hormonal status of children and their mothers.49 newborns and their mothers were examined. Two groups were identified: group 1 (Gr1) – newborns small for gestational age (n = 24), group 2 (Gr2) – newborns appropriate for gestational age (n = 25). The levels of irisin and adipocytokines in the blood serum were determined by the enzyme immunoassay.Newborns small for gestational age had significantly lower levels of leptin and IGF-1 in the umbilical cord blood compared to children with physical development corresponding to the gestational age. There were no significant differences in the irisin content of cord blood serum in newborn Gr1 compared with Gr2. The presence of significant positive correlations between the level of irisin in the umbilical cord blood of newborns small for gestational age and the body weight at birth was established. In Gr1, a positive relationship was found between the irisin levels of mothers and newborns (r = 0.518, p = 0.028). The differences in the irisin content between the groups were established, taking into account the delivery mode (p = 0.0104).The revealed statistically significant differences in the concentrations of the analyzed metabolic markers in mother–child pairs, their relationship with clinical and anthropometric parameters substantiate the possibility of using irisin and adipocytokines as predictors in predicting the formation of metabolic disorders of infants small for gestational age.

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