scholarly journals Interplay Between Angiotensin II Type 1 Receptor and Thrombin Receptor Revealed by Bioluminescence Resonance Energy Transfer Assay

2020 ◽  
Vol 11 ◽  
Author(s):  
Isra Al Zamel ◽  
Abdulrasheed Palakkott ◽  
Arshida Ashraf ◽  
Rabah Iratni ◽  
Mohammed Akli Ayoub
Keyword(s):  
Energy Transfer ◽  
Angiotensin Ii ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Thrombin Receptor ◽  
Bioluminescence Resonance Energy Transfer

scholarly journals Mapping of the Localization of Type 1 Angiotensin Receptor in Membrane Microdomains Using Bioluminescence Resonance Energy Transfer-based Sensors

2012 ◽  
Vol 287 (12) ◽  
pp. 9090-9099 ◽  
Author(s):  
András Balla ◽  
Dániel J. Tóth ◽  
Eszter Soltész-Katona ◽  
Gyöngyi Szakadáti ◽  
László Sándor Erdélyi ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Membrane Microdomains ◽  
Angiotensin Receptor ◽  
Bioluminescence Resonance Energy Transfer

scholarly journals Detection of Human Immunodeficiency Virus Type 1 Nef and CD4 Physical Interaction in Living Human Cells by Using Bioluminescence Resonance Energy Transfer

2005 ◽  
Vol 79 (13) ◽  
pp. 8629-8636 ◽  
Author(s):  
David Cluet ◽  
Christophe Bertsch ◽  
Christian Beyer ◽  
Liliane Gloeckler ◽  
Mathieu Erhardt ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Energy Transfer ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Human Cells ◽  
Bioluminescence Resonance Energy Transfer ◽  
Immunodeficiency Virus

ABSTRACT CD4 down-regulation by human immunodeficiency virus type 1 (HIV-1) Nef protein is a key function for virus virulence. This activity may be mediated by a direct Nef-CD4 interaction. We investigated the formation, in situ, of such a complex between proteins using bioluminescence resonance energy transfer technology and coimmunoprecipitations. Our data clearly demonstrate that Nef and CD4 interact in intact human cells. Moreover, our results clearly indicate that the dileucine motif of the CD4 cytoplasmic domain, critical for the Nef-induced CD4 down-regulation, is not implicated in the Nef/CD4 complex formation in the cellular context.

scholarly journals Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tomomi Kaku ◽  
Kazunori Sugiura ◽  
Tetsuyuki Entani ◽  
Kenji Osabe ◽  
Takeharu Nagai
Keyword(s):  
Energy Transfer ◽  
Mammalian Cells ◽  
Fluorescent Protein ◽  
Color Change ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Bioluminescence Resonance Energy Transfer ◽  
Bacterial Luciferase ◽  
Bacterial Bioluminescence

AbstractUsing the lux operon (luxCDABE) of bacterial bioluminescence system as an autonomous luminous reporter has been demonstrated in bacteria, plant and mammalian cells. However, applications of bacterial bioluminescence-based imaging have been limited because of its low brightness. Here, we engineered the bacterial luciferase (heterodimer of luxA and luxB) by fusion with Venus, a bright variant of yellow fluorescent protein, to induce bioluminescence resonance energy transfer (BRET). By using decanal as an externally added substrate, color change and ten-times enhancement of brightness was achieved in Escherichia coli when circularly permuted Venus was fused to the C-terminus of luxB. Expression of the Venus-fused luciferase in human embryonic kidney cell lines (HEK293T) or in Nicotiana benthamiana leaves together with the substrate biosynthesis-related genes (luxC, luxD and luxE) enhanced the autonomous bioluminescence. We believe the improved luciferase will forge the way towards the potential development of autobioluminescent reporter system allowing spatiotemporal imaging in live cells.

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