scholarly journals Conventional and Molecular Breeding Tools for Accelerating Genetic Gain in Faba Bean (Vicia Faba L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Kedar N. Adhikari ◽  
Hamid Khazaei ◽  
Lamiae Ghaouti ◽  
Fouad Maalouf ◽  
Albert Vandenberg ◽  
...  
Keyword(s):  
Grain Yield ◽  
Faba Bean ◽  
Genetic Gain ◽  
Dna Markers ◽  
Abiotic Stresses ◽  
Selection Process ◽  
Abiotic Stress Tolerance ◽  
Ascochyta Blight ◽  
Grain Legume ◽  
Breeding Programs

Faba bean is a cool-season grain legume crop, which is grown worldwide for food and feed. Despite a decrease in area under faba bean in the past, the interest in growing faba bean is increasing globally due to its high seed protein content and its excellent ecological service. The crop is, however, exposed to diverse biotic and abiotic stresses causing unstable, low grain yield. Although, sources of resistance to main diseases, such as ascochyta blight (Ascochyta fabae Speg.), rust (Uromyces viciae-fabae (Pers.) Schroet.), chocolate spot (Botrytis fabae Sard.) and gall disease (Physioderma viciae), have been identified, their resistance is only partial and cannot prevent grain yield losses without agronomical practices. Tightly associated DNA markers for host plant resistance genes are needed to enhance the level of resistance. Less progress has been made for abiotic stresses. Different breeding methods are proposed, but until now line breeding, based on the pedigree method, is the dominant practice in breeding programs. Nonetheless, the low seed multiplication coefficient and the requirement for growing under insect-proof enclosures to avoid outcrossing hampers breeding, along with the lack of tools such as double haploid system and cytoplasmic male sterility. This reduces breeding population size and speed of breeding hence the chances of capturing rare combinations of favorable alleles. Availability and use of the DNA markers such as vicine-convicine (vc−) and herbicide tolerance in breeding programs have encouraged breeders and given confidence in marker assisted selection. Closely linked QTL for several biotic and abiotic stress tolerance are available and their verification and conversion in breeder friendly platform will enhance the selection process. Recently, genomic selection and speed breeding techniques together with genomics have come within reach to accelerate the genetic gains in faba bean. Advancements in genomic resources with other breeding tools, methods and platforms will enable to accelerate the breeding process for enhancing genetic gain in this species.

Abiotic Stress Tolerance in Soybean : Regulated by ncRNA

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
YASIN JESHIMA KHAN ◽  
HUSNARA Tyagi ◽  
Anil kumar Singh ◽  
Santosh kumar. Magadum
Keyword(s):  
Abiotic Stresses ◽  
Target Genes ◽  
Abiotic Stress Tolerance ◽  
Crop Improvement ◽  
Vital Role ◽  
Grain Legume ◽  
Biological Processes ◽  
Small Interfering Rnas ◽  
Cellular Environment ◽  
Non Coding Rnas

Plants respond through a cascade of reactions resulting in varied cellular environment leading to alterations in the patterns of protein expression resulting in phonotypic changes. Single cell genomics and global proteomics came out to be powerful tools and efficient techniques in studying stress tolerant plants. Non-coding RNAs are a distinct class of regulatory RNAs in plants and animals that control a variety of biological processes. Small ncRNAs play a vital role in post transcriptional gene regulation by either translational repression or by inducing mRNA cleavage. The major classes of small RNAs include microRNAs (miRNAs) and small interfering RNAs (siRNAs), which differ in their biogenesis. miRNAs control the expression of cognate target genes by binding to complementary sequences, resulting in cleavage or translational inhibition of the target RNAs. siRNAs too have a similar structure, function, and biogenesis like miRNAs but are derived from long double-stranded RNAs and can often direct DNA methylation at target sequences.In this review, we focus on the involvement of ncRNAs in comabting abiotic stresses of soybean. This review emphasis on previously known miRNAs as they play important role in several abiotic stresses like drought, salinity, chilling and heat stress by their diverse roles in mediating biological processes like gene expression, chromatin formation, defense of genome against invading viruses. This review attempts to elucidate the various kinds of non-coding RNAs explored, their discovery, biogenesis, functions, and response for different type of abiotic stresses and future aspects for crop improvement in the context of soybean, a representative grain legume.

Characterisation of genetic diversity and DNA fingerprinting of Australian chickpea (Cicer arietinum L.) cultivars using MFLP markers

2008 ◽  
Vol 59 (8) ◽  
pp. 707 ◽  
Author(s):  
R. Lin ◽  
H. Yang ◽  
T. N. Khan ◽  
K. H. M. Siddique ◽  
G. Yan
Keyword(s):  
Genetic Diversity ◽  
Cicer Arietinum ◽  
Dna Markers ◽  
Fragment Length Polymorphism ◽  
Grain Legume ◽  
Dna Fingerprints ◽  
Breeding Programs ◽  
Cicer Arietinum L ◽  
The World ◽  
Chickpea Cultivars

Chickpea (Cicer arietinum L.) is one of the major grain legume crops in the world. In this study, the genetic diversity of 24 Australian chickpea cultivars released between 1987 and 2005 was investigated with microsatellite-anchored fragment length polymorphism (MFLP) DNA markers. Among the cultivars examined, 30 cultivar-specific markers were identified and all were unequivocally identified using the DNA fingerprints developed in this study. Most of the cultivars were grouped into two major clusters; cv. Flipper was separated from the rest based on total character differences of DNA polymorphism. The MFLP approach proved suitable in the analysis of genetic diversity among the chickpea cultivars studied and the genetic relationship identified will be useful for chickpea breeding programs in selecting parent materials.

scholarly journals Modeling Illustrates That Genomic Selection Provides New Opportunities for Intercrop Breeding

2021 ◽  
Vol 12 ◽  
Author(s):  
Jon Bančič ◽  
Christian R. Werner ◽  
R. Chris Gaynor ◽  
Gregor Gorjanc ◽  
Damaris A. Odeny ◽  
...  
Keyword(s):  
Grain Yield ◽  
Genetic Correlation ◽  
Genomic Selection ◽  
Genetic Gain ◽  
Phenotypic Selection ◽  
Breeding Program ◽  
Weed Suppression ◽  
Selection Strategy ◽  
Agricultural Practice ◽  
Breeding Programs

Intercrop breeding programs using genomic selection can produce faster genetic gain than intercrop breeding programs using phenotypic selection. Intercropping is an agricultural practice in which two or more component crops are grown together. It can lead to enhanced soil structure and fertility, improved weed suppression, and better control of pests and diseases. Especially in subsistence agriculture, intercropping has great potential to optimize farming and increase profitability. However, breeding for intercrop varieties is complex as it requires simultaneous improvement of two or more component crops that combine well in the field. We hypothesize that genomic selection can significantly simplify and accelerate the process of breeding crops for intercropping. Therefore, we used stochastic simulation to compare four different intercrop breeding programs implementing genomic selection and an intercrop breeding program entirely based on phenotypic selection. We assumed three different levels of genetic correlation between monocrop grain yield and intercrop grain yield to investigate how the different breeding strategies are impacted by this factor. We found that all four simulated breeding programs using genomic selection produced significantly more intercrop genetic gain than the phenotypic selection program regardless of the genetic correlation with monocrop yield. We suggest a genomic selection strategy which combines monocrop and intercrop trait information to predict general intercropping ability to increase selection accuracy in the early stages of a breeding program and to minimize the generation interval.

scholarly journals GENETIC GAINS IN THE POPCORN POPULATION UENF-14: DEVELOPING THE NINTH GENERATION OF INTRAPOPULATION RECURRENT SELECTION

2019 ◽  
Vol 32 (3) ◽  
pp. 625-633
Author(s):  
AMANDA GONÇALVES GUIMARÃES ◽  
ANTÔNIO TEIXEIRA DO AMARAL JÚNIOR ◽  
GUILHERME FERREIRA PENA ◽  
JANEO EUSTÁQUIO DE ALMEIDA FILHO ◽  
MESSIAS GONZAGA PEREIRA ◽  
...  
Keyword(s):  
Grain Yield ◽  
Indirect Effects ◽  
Recurrent Selection ◽  
Selection Process ◽  
Selection Index ◽  
Average Height ◽  
Direct And Indirect Effects ◽  
Genetic Progress ◽  
Breeding Programs ◽  
Selection Of

ABSTRACT The occurrence of negative correlations between grain yield (GY) and popping expansion (PE) in popcorn complicates the selection process. In an attempt to overcome this inconvenience, we proposed the use of the importance characteristic volume per popcorn per hectare (PV). The objectives of this study were to develop the ninth cycle of the UENF-14 popcorn population, to estimate the direct and indirect effects of PV and to verify the genetic progress among all selective cycles. We obtained and evaluated 200 inbred progenies in randomised blocks arranged in replicates within sets, with nine sets consisting of 25 progenies and one set with all previous eight cycles with three replicates. The average height of the plants, prolificacy, 100-grain weight, grain yield, grain popping expansion and volume per popcorn per hectare were evaluated. Track analysis was performed to determine the direct and indirect effects, and the Mulamba and Mock selection index was calculated for the selection of the 40 superior progenies. There was genetic variability among progenies in all evaluated traits, with a cause-and-effect relationship between PV with GY and PE, allowing simultaneous gains with indirect selection. The selection of the best progenies was more effective when using arbitrarily assigned weights, which provided higher predicted gains for PV (20.73%). The means obtained in all cycles indicated increases, demonstrating the efficiency of using continuous recurrent selection in popcorn breeding programs.

scholarly journals Strategies of Preserving Genetic Diversity While Maximizing Genetic Response From Implementing Genomic Selection in Pulse Breeding Programs

2021 ◽  
Author(s):  
Yongjun Li ◽  
Sukhjiwan Kaur ◽  
Luke W. Pembleton ◽  
Hossein Valipour-Kahrood ◽  
Garry M. Rosewarne ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Grain Yield ◽  
Genomic Selection ◽  
Genetic Gain ◽  
Complex Traits ◽  
Phenotypic Selection ◽  
Breeding Cycle ◽  
Environment Interaction ◽  
Breeding Programs ◽  
Diversity Loss

Abstract Using a stochastic computer simulation, we investigated the benefit of optimization strategies in the context of genomic selection (GS) for pulse breeding programs. We simulated GS for moderately complex to highly complex traits such as disease resistance, grain weight and grain yield in multiple environments with a high level of genotype-by-environment interaction for grain yield. GS led to higher genetic gain per unit of time and higher genetic diversity loss than phenotypic selection by shortening the breeding cycle time. The genetic gain obtained from selecting the segregating parents early in the breeding cycle (at F1 or F2 stages) was substantially higher than selecting at later stages even though prediction accuracy was moderate. Increasing the number of F1 intercross (F1i) families and keeping the total number of progeny of F1i families constant, we observed a decrease in genetic gain and increase in genetic diversity. Whereas increasing the number of progeny per F1i family while keeping a constant number of F1i families increased rate of genetic gain and had higher genetic diversity loss per unit of time. Adding 50 F2 family phenotypes to the training population increased the accuracy of GEBVs and genetic gain per year and decreased the rate of genetic diversity loss. Genetic diversity could be preserved by applying a strategy that restricted both the percentage of alleles fixed and the average relationship of the group of selected parents to preserve long-term genetic improvement in the pulse breeding program.

scholarly journals GENETIC GAINS AND SELECTION ADVANCES OF THE UENF-14 POPCORN POPULATION

2018 ◽  
Vol 31 (2) ◽  
pp. 271-278 ◽  
Author(s):  
AMANDA GONÇALVES GUIMARÃES ◽  
ANTÔNIO TEIXEIRA DO AMARAL JÚNIOR ◽  
VALTER JÁRIO DE LIMA ◽  
JHEAN TORRES LEITE ◽  
CARLOS ALBERTO SCAPIM ◽  
...  
Keyword(s):  
Grain Yield ◽  
Genetic Gain ◽  
Recurrent Selection ◽  
Selection Index ◽  
Economic Importance ◽  
Average Height ◽  
Breeding Programs ◽  
Genetic Gains ◽  
Full Sibling ◽  
Selection Of

ABSTRACT Recurrent selection can generate successive gains for characters of economic interest without causing genetic narrowing in the population. However, it has rarely been used in breeding programs in popcorn, especially when using full-sibling progenies to generate more expressive gains. The objective of this study was to estimate the genetic gain of the UENF-14 popcorn population through recurrent selection, and verify the evolution of the gains between the selection cycles four and eight. A total of 200 full-sibling progenies were evaluated in randomized blocks arranged in eight sets within three replicates in two environments; each set containing twenty-five progenies and six controls (selection cycles 4, 5, 6 and 7 of the UENF-14, BRS-Angela and IAC-125). The average height, prolificacy, 100-grain weight, ear weight, grain yield, and grain popping expansion of the plants were evaluated. In the selection of the thirty superior progenies for the eighth cycle, the Mulamba and Mock selection index was used, which generated estimated genetic gains of 4.60 for grain yield and 3.61% for popping expansion. The grain yield increased 111.99 kg ha-1 and the popping expansion increased 1.75 mL g-1 per cycle. The evolution of the cycles resulted in an accumulated genetic gain for the main characters of economic importance, allowing the prediction of success in the continuity of the recurrent selection.

scholarly journals Modelling illustrates that genomic selection provides new opportunities for intercrop breeding

2020 ◽  
Author(s):  
Jon Bančič ◽  
Christian Werner ◽  
Chris Gaynor ◽  
Gregor Gorjanc ◽  
Damaris Odeny ◽  
...  
Keyword(s):  
Grain Yield ◽  
Genetic Correlation ◽  
Genomic Selection ◽  
Genetic Gain ◽  
Phenotypic Selection ◽  
Breeding Program ◽  
Weed Suppression ◽  
Selection Strategy ◽  
Agricultural Practice ◽  
Breeding Programs

AbstractIntercrop breeding programs using genomic selection can produce faster genetic gain than intercrop breeding programs using phenotypic selection. Intercropping is an agricultural practice in which two or more component crops are grown together. It can lead to enhanced soil structure and fertility, improved weed suppression, and better control of pests and diseases. Especially in subsistence agriculture, intercropping has great potential to optimise farming and increase profitability. However, breeding for intercrop varieties is complex as it requires simultaneous improvement of two or more component crops that combine well in the field. We hypothesize that genomic selection can significantly simplify and accelerate the process of breeding crops for intercropping. Therefore, we used stochastic simulation to compare four different intercrop breeding programs implementing genomic selection and an intercrop breeding program entirely based on phenotypic selection. We assumed three different levels of genetic correlation between monocrop grain yield and intercrop grain yield to investigate how the different breeding strategies are impacted by this factor. We found that all four simulated breeding programs using genomic selection produced significantly more intercrop genetic gain than the phenotypic selection program regardless of the genetic correlation with monocrop yield. We suggest a genomic selection strategy which combines monocrop and intercrop trait information to predict general intercropping ability to increase selection accuracy in early stages of a breeding program and to minimize the generation interval.

scholarly journals Enhanced Abiotic Stress Tolerance of Vicia faba L. Plants Heterologously Expressing the PR10a Gene from Potato

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 173
Author(s):  
Abeer F. Desouky ◽  
Ahmed H. Ahmed ◽  
Hartmut Stützel ◽  
Hans-Jörg Jacobsen ◽  
Yi-Chen Pao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Vicia Faba ◽  
Faba Bean ◽  
Abiotic Stress Tolerance ◽  
Wild Type ◽  
Stress Injury ◽  
Bean Plants ◽  
Vicia Faba L

Pathogenesis-related (PR) proteins are known to play relevant roles in plant defense against biotic and abiotic stresses. In the present study, we characterize the response of transgenic faba bean (Vicia faba L.) plants encoding a PR10a gene from potato (Solanum tuberosum L.) to salinity and drought. The transgene was under the mannopine synthetase (pMAS) promoter. PR10a-overexpressing faba bean plants showed better growth than the wild-type plants after 14 days of drought stress and 30 days of salt stress under hydroponic growth conditions. After removing the stress, the PR10a-plants returned to a normal state, while the wild-type plants could not be restored. Most importantly, there was no phenotypic difference between transgenic and non-transgenic faba bean plants under well-watered conditions. Evaluation of physiological parameters during salt stress showed lower Na+-content in the leaves of the transgenic plants, which would reduce the toxic effect. In addition, PR10a-plants were able to maintain vegetative growth and experienced fewer photosystem changes under both stresses and a lower level of osmotic stress injury under salt stress compared to wild-type plants. Taken together, our findings suggest that the PR10a gene from potato plays an important role in abiotic stress tolerance, probably by activation of stress-related physiological processes.

scholarly journals Improvement of Maize Productivity and N Use Efficiency in a No-Tillage Irrigated Farming System: Effect of Cropping Sequence and Fertilization Management

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1459
Author(s):  
Heba S. A. Salama ◽  
Ali I. Nawar ◽  
Hassan E. Khalil ◽  
Ahmed M. Shaalan
Keyword(s):  
Grain Yield ◽  
Faba Bean ◽  
Farming System ◽  
No Tillage ◽  
Maize Crop ◽  
Egyptian Clover ◽  
Maize Grain Yield ◽  
Use Efficiency ◽  
Maize Grain ◽  
N Use

The sequence of the preceding crops in a no-tillage farming system, could interact with the integrated use of mineral and organic nitrogen (N) sources in a way that improves the growth and productivity of the terminal maize crop, meanwhile, enhancing its N use efficiency (NUE). In the current study, six legume-cereal crop sequences, including faba bean, soybean, Egyptian clover, wheat, and maize were evaluated along two experimental rotations that ended up by planting the terminal maize crop. In addition, the effects of applying variable mineral nitrogen (MN) rates with and without the incorporation of farmyard manure (FYM) on the productive performance of maize and its NUE were tested. The field experiments were conducted in a no-tillage irrigated farming system in Northern Egypt, a location that is characterized by its arid, Mediterranean climate. Results revealed that increasing the legume component in the evaluated crop sequences, up to 75%, resulted in improved maize ear leaf area, 1000-grain weight, and harvest index, thus, a higher final grain yield, with the inclusion of Egyptian clover was slightly better than faba bean. Comparing the crop sequences with 50% legume contribution uncovered the positive effects of soybean preceding crop on the terminal maize crop. Substituting 25% of the applied MN with FYM resulted in similar maize yields to the application of the equivalent 100% MN rates. The fertilizer treatments significantly interacted with the crop sequences in determining the maize grain yield, where the highest legume crop contribution in the crop sequence (75%) equalized the effects of the different fertilizer treatments on maize grain yield. The integrated use of FYM with MN in maize fertilization improved the NUE compared to the application of MN alone. Comparing fertilization treatments with similar MN content, with and without FYM, revealed that the difference in NUE was attributed to the additional amount of FYM. In similar conditions to the current study, it is recommended to grow faba bean two years before maize, while Egyptian clover could be grown directly preceding maize growth, with frequent inclusion of soybean in the sequence, this could be combined with the application of an average of 200 kg MN ha−1 in addition to FYM.

scholarly journals Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts

2021 ◽  
Author(s):  
Johannes W. Debler ◽  
Bernadette M. Henares ◽  
Robert C. Lee
Keyword(s):  
Broad Spectrum ◽  
Faba Bean ◽  
Fluorescent Protein ◽  
Transient Gene Expression ◽  
Low Frequency ◽  
Effector Proteins ◽  
Field Pea ◽  
Grain Legume ◽  
Pathogen Effectors ◽  
Green Fluorescent

Abstract Key message Modified pEAQ-HT-DEST1 vectors were used for agroinfiltration in legumes. We demonstrate protein expression and export in pea, lentil, and faba bean; however, the method for chickpea was not successful. Abstract Agroinfiltration is a valuable research method for investigating virulence and avirulence effector proteins from pathogens and pests, where heterologous effector proteins are transiently expressed in plant leaves and hypersensitive necrosis responses and other effector functions can be assessed. Nicotiana benthamiana is widely used for agroinfiltration and the characterisation of broad-spectrum effectors. The method has also been used in other plant species including field pea, but not yet developed for chickpea, lentil, or faba bean. Here, we have modified the pEAQ-HT-DEST1 vector for expression of 6 × histidine-tagged green-fluorescent protein (GFP) and the known necrosis-inducing broad-spectrum effector necrosis and ethylene-inducing peptide (Nep1)-like protein (NLP). Modified pEAQ-based vectors were adapted to encode signal peptide sequences for apoplast targeting of expressed proteins. We used confocal microscopy to assess the level of GFP expression in agroinfiltrated leaves. While at 3 days after infiltration in N. benthamiana, GFP was expressed at a relatively high level, expression in field pea and faba bean at the same time point was relatively low. In lentil, an expression level of GFP similar to field pea and faba bean at 3 days was only observed after 5 days. Chickpea leaf cells were transformed at low frequency and agroinfiltration was concluded to not be successful for chickpea. We concluded that the pEAQ vector is suitable for testing host-specific effectors in field pea, lentil, and faba bean, but low transformation efficiency limits the utility of the method for chickpea.

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