scholarly journals Radix Puerarin Extract (Puerarin) Could Improve Meat Quality of Heat-Stressed Beef Cattle Through Changing Muscle Antioxidant Ability and Fiber Characteristics

2021 ◽  
Vol 7 ◽  
Author(s):  
Yanjiao Li ◽  
Hanle Shang ◽  
Xianghui Zhao ◽  
Mingren Qu ◽  
Tao Peng ◽  
...  

The experiment was conducted to investigate the effects of dietary supplementation with Puerarin on meat quality, muscle antioxidant ability, and muscle fiber characteristics of beef cattle under a hot environment in summer. Thirty-two 15 ± 1.5-month-old Jinjiang bulls (291.65 ± 8.84 kg) were randomly divided into four groups with dietary Puerarin at 0 (control), 200 (Pue200), 400 (Pue400), and 800 (Pue800) mg/kg in the feed concentrate (n = 8). The feeding trial lasted for 60 days after a 10-day adaptation period (July 1–September 8); the average values of temperature, relative humidity and temperature, and humidity index were 30.68°C, 68.05%, and 81.81, respectively. The growth performance on day 40 of the experiment period was calculated. After 60 days' experimental period, four Jinjiang cattle per treatment from the control group, Pue400 group, and Pue800 group were slaughtered. Compared with the control group, the Pue400 and Pue800 groups improved the growth performance of beef cattle; the Pue800 group elevated the activities of superoxide dismutase, total antioxidant capacity, and glutathione peroxidase in the M. longissimus thoracis (LT) muscle. In the control group, the cell membrane was incomplete, and most of the mitochondria were elongated and in a fission state, while in the Pue400 and Pue800 groups, the cell membrane was clear and complete, and the mitochondria presented with round and oval shapes. Compared with the control group, the Pue400 and Pue800 groups reduced the shear force of the LT muscle, and the Pue400 group decreased the muscle fiber diameter and the myosin heavy-chain (MyHC)-IIb gene expression. Furthermore, the Pue400 and Pue800 groups decreased the ratio of AMP/ATP, the Pue800 group reduced the AMP-activated protein kinase α2 mRNA expression, and the Pue400 group improved the nuclear respiratory factor 1 mRNA expression. These results indicated that dietary supplementation with Puerarin might be beneficial to the meat quality of heat-stressed beef cattle by improving muscle antioxidant ability and reducing the MyHC-IIb muscle fiber composition. Based on the results of this study, we recommended 400 mg/kg Puerarin in the feed concentrate of beef cattle (~300 kg) for mitigation of heat stress.

2018 ◽  
Vol 58 (5) ◽  
pp. 965 ◽  
Author(s):  
M. E. Abd El-Hack ◽  
E. A. Ashour ◽  
Gh. M. Elaraby ◽  
A. O. Osman ◽  
M. Arif

The present study aimed to investigate the effects of dietary supplementation with powdered peanut skin (PS) as natural antioxidant and growth promoter on growth performance, carcass characteristics, serum metabolites and meat quality of Cobb broiler chickens. A total of 240 unsexed Cobb 500 1-week-old broiler chicks were randomly assigned to four treatment groups with six replicates. The dietary treatments consisted of the basal diet as control and PS groups (1.0, 2.0, and 3.0 PS g/kg). Results showed that the highest values (P < 0.01) of live bodyweight and bodyweight gain were observed in the control group at 3 weeks of age and during the period of 1–3 weeks of age, respectively compared with all levels of PS. During 1–3 weeks of age, feed intake per day chicks fed the diet containing PS levels decreased numerically (P > 0.01) with increased levels of PS compared with the control. Supplementing 1.0 or 3.0 g/kg of PS insignificantly enhanced feed conversion ratio at 1–6 weeks of age. Dietary treatments significantly depressed giblets (P = 0.002), thigh (P = 0.0.027) and abdominal fat percentages (P > 0.0.1), however, increased (P = 0.004) breast percentage of broiler chicks at 6 weeks of age. The lowest value of serum creatinine and urea achieved by chicks fed diet contained 3.0 PS g/kg diet. Also, high density lipoprotein cholesterol in addition to triglycerides were significantly (P < 0.05 and P < 0.01) decreased with increasing level of PS supplementation. It can be concluded that PS as a phytogenic additive could be used as a supplementation to broiler diets to lower abdominal fat in carcass and cholesterol, creatinin and urea in blood. Sensory characteristics were not affected except that appearance was improved. Water-holding capacity was also improved.


2019 ◽  
Vol 157 (2) ◽  
pp. 169-175
Author(s):  
H. Liang ◽  
X. H. Zhao ◽  
K. Pan ◽  
L. J. Xu ◽  
Z. H. Yi ◽  
...  

AbstractAn experiment was conducted to determine the effects of supplementing different amounts of daidzein in a diet on the growth performance, blood biochemical parameters and meat quality of finishing beef cattle. Thirty finishing Xianan steers were distributed in three groups equilibrated by weight and fed three different dietary treatments (concentrate ratio = 80%): (1) control; (2) 500 mg/kg daidzein and (3) 1000 mg/kg daidzein, respectively. Steers were slaughtered after an 80-day feeding trial. Results showed that daidzein supplementation had no effect on the final body weight, average daily gain and feed conversion rate of steers. Steers fed with 1000 mg/kg daidzein had greater dry matter intake than those fed with control diets. Compared with the control group, the 1000 mg/kg daidzein group had a higher fat thickness, lower shear force and lightness. The pH, drip loss, cooking loss, redness (a*), yellowness (b*), moisture, ash, crude protein and intramuscular fat of the Longissimus dorsi muscle were unaffected by daidzein supplementation. Compared with the control group, the 1000 mg/kg daidzein group significantly increased the serum concentrations of insulin, free fatty acid and Glutamic-pyruvic transaminase. The 500 mg/kg daidzein group significantly increased the serum concentration of tetraiodothyronine compared with the control group. Supplemental daidzein did not affect the blood antioxidant ability and blood immune parameters in serum. In conclusion, daidzein supplementation above 500 mg/day modifies feed intake and metabolic and hormonal profile, with positive and negative effects on meat quality.


2020 ◽  
Vol 60 (2) ◽  
pp. 288
Author(s):  
Qing-Chang Ren ◽  
Jing-Jing Xuan ◽  
Chuan-Yan Che ◽  
Xin-Chao Yan ◽  
Zhong-Ze Hu

In this trial we aimed to assess the effects of dietary supplementation of 4-O-methyl-glucuronoarabinoxylan (4OMG) on growth performance, thigh meat quality and small intestine development of female Partridge-Shank broilers. A total of 240 1-day-old female Partridge-Shank broilers were randomly distributed to four groups with three replicates of 20 within each group. Groups received either 0, 15, 20 or 25 g 4OMG/kg DM of diet. During the whole experiment of 60 days, broilers had ad libitum access to water and feed. At pen level, feed intake was recorded daily and broilers were weighed at the start and end of the experiment. For each group, three pens with a total of 20 broilers were randomly selected to determine the thigh meat quality and the small intestine development of broilers. Broilers fed diets with higher 4OMG had greater final liveweight (P = 0.004), daily bodyweight gain (P = 0.004) and gain-to-feed ratio (P &lt; 0.001), muscle pH values (P = 0.031) and redness (P = 0.001), duodenal weight index (P = 0.042), jejunal (P = 0.043) and ileal length (P = 0.049), duodenal (P &lt; 0.001) and ileal villus height (P = 0.008), but lower percentage of dead birds (P &lt; 0.001), drip loss (P = 0.042) and shear force value (P = 0.043) of the thigh muscles. These results indicate that increasing dietary supplementation of 4OMG may improve growth performance and meat quality of female Partridge-Shank broilers through better development of small intestine.


2019 ◽  
Vol 3 (2) ◽  
pp. 158-159
Author(s):  
D. Maharjan ◽  
A. Rodas-González ◽  
A. R. Tanner ◽  
V. C. Kennedy ◽  
J. D. Kirsch ◽  
...  

2021 ◽  
Author(s):  
Dingfa Wang ◽  
Luli Zhou ◽  
Hanlin Zhou ◽  
Guanyu Hou

Abstract Background: The effects of dietary supplementation with guava leaf extracts (GE) on growth performance, diarrhea and intestinal barrier function, as well as associated with its modulation of serum and fecal metabolic changes in weaned piglets challenged by enterotoxigenic Escherichia coli (ETEC) were investigated.Method: Fifty weaned piglets (Duroc × Yorkshire × Landrace) from 5 pens (2 piglets per pen) were randomly divided into five groups: blank control group (BC), negative control group (NC), or those supplemented with 50 mg kg-1 (S50), 100 mg kg-1 (S100), or 200 (S200) mg kg-1 diet GE, respectively. On day 4, all piglets (except for BC) were orally challenged with about 1.0 × 109 colony-forming units (CFU) enterotoxigenic ETEC. After 28-day trial, growth performance, diarrhea incidence, intestinal barrier function and metabolomics of serum and fecal were investigated.Results: We demonstrated that dietary supplementation with GE (50-200 mg kg-1) reduced diarrhea incidence of piglets and increased expression of intestinal tight junction proteins (ZO-1, Occludin, Claudin-1) (P < 0.05) and sodium hydrogen exchanger 3 (NHE3) (P < 0.05). Moreover, dietary supplementation with GE (50-200 mg kg-1) upregulated level of tetrahydrofolic acid (THF) and reversed higher level of nicotinamide-adenine dinucleotide phosphate (NADP) caused by ETEC in serum compared with NC group (P < 0.05), and enhanced antioxidant ability of piglets. In addition, dietary addition with GE (100 mg kg-1) reversed the lower level of L-pipecolic acid caused by ETEC in feces compared with NC group (P < 0.05), and decreased oxidative stress response of piglets. Further, there were no differences (P > 0.05) in the final weight, average daily feed intake (ADFI) and F/G among dietary groups during the overall period, and piglets in S50 group has the higher average daily gain (ADG). Conclusion: Dietary supplementation with 50-200 mg kg-1 GE reduced diarrhea incidence of weaned piglets challenged by ETEC and exhibited positive effect on improving intestinal barrier function. Meanwhile, dietary addition with GE organized and redistributed energy resources through similar or dissimilar metabolic pathways, and finally enhanced antioxidant ability of piglets challenged by ETEC.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 123-123
Author(s):  
Lihong Hao ◽  
Zeqing lu

Abstract Two experiments were conducted to evaluate the effects of feeding fermented mixed feed (FMF) on performance, meat quality, muscle fatty acids profile and antioxidant ability in finishing pigs. In experiment 1, 225 finishing pigs were allocated into 3 dietary treatments. Pigs in 3 groups were fed a basic diet or basic diet supplemented with 4% and 8% of FMF. After 35 days of the experiment, growth performance was evaluated. In experiment 2, 600 pigs were distributed into 3 treatments, which were fed with a basic diet or basic diet supplemented with 5% and 10% of FMF. At the end of the experiment, 24 pigs were slaughtered for the determination. In experiment 1, feeding 8% FMF improved (P &lt; 0.05) ADG and decreased (P &lt; 0.05) F/G compared with CON. In experiment 2, compared with CON, dietary supplementation with 10% FMF decreased (P &lt; 0.05) F/G. Moreover, the ADFI and ADG were tended to be higher in pigs fed the FMF diet. Feeding 10% FMF increased (P &lt; 0.05) loin muscle area, a* value, marbling score, intramuscular fat (IMF), and inosine acid (IMP) and decreased (P &lt; 0.05) drip loss and shear force of longissimus thoracis (LT) muscle. The percentages of C18:1ω-9c, C18:2ω-6c, and C20:4ω-6c in LT muscle were increased (P &lt; 0.05) in pigs fed 10 % FMF diet. Furthermore, greater (P &lt; 0.05) SOD and GSH-Px activities were observed in 10 % FMF group in serum and LT muscle of pigs. Collectively, the present study suggested that dietary FMF supplementation improved the growth performance, meat quality parameters, unsaturated fatty acids proportions, and antioxidant ability.


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