scholarly journals Hepatic Injury Induced by Dietary Energy Level via Lipid Accumulation and Changed Metabolites in Growing Semi-Fine Wool Sheep

2021 ◽  
Vol 8 ◽  
Author(s):  
Benchu Xue ◽  
Qionghua Hong ◽  
Xiang Li ◽  
Mingli Lu ◽  
Jia Zhou ◽  
...  
Keyword(s):  
Energy Level ◽  
Lipid Accumulation ◽  
Hepatic Injury ◽  
Energy Levels ◽  
High Energy ◽  
Protein Carbonyl ◽  
Low Energy ◽  
Metabolic Energy ◽  
Dietary Energy ◽  
Tnf Α

Liver injury threatens the overall health of an organism, as it is the core organ of the animal body. Liver metabolism is affected by numerous factors, with dietary energy level being a crucial one. Therefore, the present study aimed to evaluate hepatic injury and to describe its metabolic mechanism in ruminants fed diets with different dietary energy levels. A total of 25 Yunnan semi-fine wool sheep were fed diets with five dietary metabolic energy levels and were randomly assigned to five groups as follows: low energy (LE), medium–low energy (MLE), medium energy (ME), medium–high energy (MHE), and high energy (HE). The results revealed that the average optical density (AOD) of lipid droplets in the LE, MLE, and HE groups was higher than that in the ME and MHE groups. The enzyme activity of alanine aminotransferase (ALT) was the lowest in the ME group. An increase in dietary energy level promoted the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity and altered the malondialdehyde (MDA) and protein carbonyl (PCO) concentration quadratically. In addition, both high and low dietary energy levels upregulated the mRNA abundance of proinflammatory cytokine interleukin (IL)-1β, nuclear factor-kappa B (NF-κB), and tumor necrosis factor (TNF)-α. Metabonomic analysis revealed that 142, 77, 65, and 108 differential metabolites were detected in the LE, MLE, MHE, and HE groups, compared with ME group respectively. These metabolites were involved in various biochemical pathways, such as glycolipid, bile acid, and lipid metabolism. In conclusion, both high and low dietary energy levels caused hepatic injury. Section staining and metabonomic results revealed that hepatic injury might be caused by altered metabolism and lipid accumulation induced by lipid mobilization.

scholarly journals Ruminal Microbiota and Fermentation in Response to Dietary Protein and Energy Levels in Weaned Lambs

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 109 ◽  
Author(s):  
Xiaokang Lv ◽  
Kai Cui ◽  
Minli Qi ◽  
Shiqin Wang ◽  
Qiyu Diao ◽  
...  
Keyword(s):  
Growth Performance ◽  
Energy Levels ◽  
High Energy ◽  
Metabolizable Energy ◽  
Low Energy ◽  
Dietary Energy ◽  
Ruminal Fermentation ◽  
Protein Levels ◽  
Energy Group ◽  
Low Energy Diet

Supplying sufficient nutrients, such as dietary energy and protein, has a great effect on the growth and rumen development of ruminants. This study was conducted to evaluate the effects of dietary energy and protein levels on growth performance, microbial diversity, and structural and physiological properties of the rumen in weaned lambs. A total of 64 two-month-old Hu lambs were randomly allotted to 2 × 2 factorial arrangements with four replicates and with four lambs (half male and half female) in each replicate. The first factor was two levels of dietary metabolizable energy (ME) density (ME = 10.9 MJ/Kg or 8.6 MJ/Kg), and the second factor was two levels of dietary crude protein (CP) content (CP = 15.7% or 11.8%). The trial lasted for 60 days. A low dietary energy level restrained the growth performance of lambs (p < 0.05). The ruminal concentration of acetate and the ratio of acetate to propionate increased but the propionate concentration decreased significantly with the low energy diet. However, the rumen morphology was not affected by the diet energy and protein levels. Moreover, a low energy diet increased ruminal bacterial diversity but reduced the abundance of the phylum Proteobacteria (p < 0.05) and genus Succinivibrionaceae_uncultured (p < 0.05), which was associated with the change in ruminal fermentation phenotypes. By indicator species analysis, we found three indicator OTUs in the high energy group (Succinivibrionaceae_uncultured, Veillonellaceae_unclassified and Veillonellaceae_uncultured (p < 0.01)) and two indicator OTUs in the low energy group (Bacteroidales_norank and Lachnospiraceae_uncultured (p < 0.01)). In conclusion, these findings added new dimensions to our understanding of the diet effect on rumen microbial community and fermentation response, and are of great significance for establishing the optimal nutrient supply strategy for lambs.

scholarly journals Dietary Energy Levels Affect Growth Performance through Growth Hormone and Insulin-Like Growth Factor 1 in Yak (Bos grunniens)

Animals ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 39 ◽  
Author(s):  
Chao Yang ◽  
Jianbo Zhang ◽  
Anum Ahmad ◽  
Pengjia Bao ◽  
Xian Guo ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Energy Level ◽  
Growth Performance ◽  
Growth Hormone Receptor ◽  
Energy Levels ◽  
High Energy ◽  
Dietary Energy ◽  
Insulin Like Growth Factor ◽  
Feed Conversion

The objective of this study was to investigate the effects of different dietary energy levels on serum concentrations of growth hormone (GH) and insulin-like growth factor 1 (IGF-1), as well as gene expression of their associated binding proteins and receptors in yak. Fifteen adult male yaks with BW of 276.1 ± 3.5 kg were allotted in three dietary groups and were fed with low (LE), medium (ME), and high energy (HE) level diet having different NEg of 5.5 MJ/kg, 6.2 MJ/kg, 6.9 MJ/kg, respectively. The effects of these treatments on ADG, BW, ADFI, and feed conversion ratio were significant (p < 0.05) throughout the experimental period. Serum GH concentration decreased (p < 0.05) with an increase in dietary energy level on d 30 and d 60. While IGF-1 concentration was higher (p < 0.05) in ME group, as compared to LE and HE groups on d 60. The expression level of growth hormone receptor (GHR) was decreased (p < 0.001) and IGF-1 was increased with the increase in the dietary energy level. The relative expression of insulin-like growth factor binding protein 3 (IGFBP-3) was higher (p < 0.001) in ME and HE groups, except the LE group. In conclusion, our findings provide a first insight into the combined effect of GH and IGF-1 in controlling the metabolism and productivity of yak. It also showed that medium energy level diet contributed to promote growth performance of yak during the cold season.

scholarly journals High-energy diet does not overcome the negative impact of conjugated linoleic acid on young broiler performance

2020 ◽  
Vol 43 ◽  
pp. e51128
Author(s):  
Katia Maria Cardinal ◽  
Júlia Guazzelli Pezzali ◽  
Lucas de Marques Vilella ◽  
Priscila de Oliveira Moraes ◽  
Andréa Machado Leal Ribeiro
Keyword(s):  
Linoleic Acid ◽  
Energy Level ◽  
Growth Performance ◽  
Conjugated Linoleic Acid ◽  
Negative Impact ◽  
Energy Levels ◽  
High Energy ◽  
Dietary Energy ◽  
Broiler Performance ◽  
Negative Effect

The aim of this study was to evaluate the effect of conjugated linoleic acid (CLA) supplementation in diets with different energy levels in broiler performance. Birds were offered a starter (1-21 d), grower (22-35 d) and finisher (36-42 d) diets; wherein soybean oil was replaced by CLA. The study consisted of a 3 × 2 factorial arrangement with two CLA levels (0 and 1%) and three energy levels (3050, 3100 and 3150 ME kg-1 diet). During the grower and finisher periods, birds were fed diets with same energy level and CLA supplementation was maintained the same. Growth performance was assessed weekly, and carcass and cuts yield were assessed at 42d. Interaction effect of CLA by energy level was observed in broiler performance and carcass yield throughout the study (p > 0.05). During the overall period (1-42 d) broiler performance was not affected by CLA (p > 0.05).However, CLA supplementation (1%) decreased weight gain (p < 0.05) at 21d, regardless of energy level, with no effects on feed intake and feed conversation rate (p > 0.05). The increase in dietary energy was not able to compensate the negative effect on growth performance of broilers supplemented with 1% CLA at the starter period.

scholarly journals Changes in white muscle transcriptome induced by dietary energy levels in two lines of rainbow trout (Oncorhynchus mykiss) selected for muscle fat content

2009 ◽  
Vol 103 (5) ◽  
pp. 629-642 ◽  
Author(s):  
Catherine-Ines Kolditz ◽  
Elisabeth Plagnes-Juan ◽  
Edwige Quillet ◽  
Florence Lefèvre ◽  
Françoise Médale
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
White Muscle ◽  
Energy Levels ◽  
High Energy ◽  
Fat Content ◽  
Fat Deposition ◽  
Dietary Energy ◽  
Expression Change ◽  
Vldl Receptor

Energy intake and genetic background are major determinants of muscle fat content in most animals, including man. We combined genetic selection and dietary energy supply to study the metabolic pathways involved in genetic and nutritional control of fat deposition in the muscle of rainbow trout (Oncorhynchus mykiss). Two experimental lines of rainbow trout, selected for lean (L) or fat (F) muscle, were fed with diets containing either 10 or 23 % lipids from the first feeding, up to 6 months. At the end of the trial, trout exhibited very different values of muscle fat content (from 4·2 to 10·1 % wet weight). Using microarrays made from a rainbow trout multi-tissue cDNA library, we analysed the molecular changes occurring in the muscle of the two lines when fed the low-energy or high-energy diet. The results from microarray analysis revealed that eleven metabolism-related genes were differentially expressed according to the diet while selection resulted in expression change for twenty-six genes. The most striking observation was the increased level of transcripts encoding the VLDL receptor and fatty acid translocase/CD36 following both the high-fat diet and upward selection for muscle fat content, suggesting that these two genes are relevant molecular markers of fat deposition in the white muscle of rainbow trout.

scholarly journals Radiation Sterilization: Dose Is Dose

2020 ◽  
Vol 54 (s1) ◽  
pp. 45-52
Author(s):  
Joyce M. Hansen ◽  
Niki Fidopiastis ◽  
Trabue Bryans ◽  
Michelle Luebke ◽  
Terri Rymer
Keyword(s):  
Energy Level ◽  
Energy Levels ◽  
Radiation Energy ◽  
Biological Indicators ◽  
High Energy ◽  
Microbial Inactivation ◽  
Radiation Sterilization ◽  
Dose Rates ◽  
Radiation Sources ◽  
Radiation Sterilization Dose

Abstract In the radiation sterilization arena, the question often arises as to whether radiation resistance of microorganisms might be affected by the energy level of the radiation source and the rate of the dose delivered (kGy/time). The basis for the question is if the microbial lethality is affected by the radiation energy level and/or the rate the dose is delivered, then the ability to transfer dose among different radiation sources could be challenged. This study addressed that question by performing a microbial inactivation study using two radiation sources (gamma and electron beam [E-beam]), two microbial challenges (natural product bioburden and biological indicators), and four dose rates delivered by three energy levels (1.17 MeV [gamma], 1.33 MeV [gamma], and 10 MeV [high-energy E-beam]). Based on analysis of the data, no significant differences were seen in the rate of microbial lethality across the range of radiation energies evaluated. In summary, as long as proof exists that the specified dose is delivered, dose is dose.

scholarly journals Effects of dietary energy levels using calcium salts of fatty acids on nutritive value of diets and milk quality in peripartum dairy goats

2014 ◽  
Vol 38 (3) ◽  
pp. 286-294 ◽  
Author(s):  
Rodrigo de Souza ◽  
Claudete Regina Alcalde ◽  
Bruna Hygino ◽  
Bruna Susan de Labio Molina ◽  
Geraldo Tadeu dos Santos ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Energy Level ◽  
Milk Fat ◽  
Dry Matter ◽  
Nutritive Value ◽  
Energy Levels ◽  
Milk Quality ◽  
Dietary Energy ◽  
Dairy Goats

Peripartum is a critical period for dairy goats, as dry matter intake (DMI) is not enough to supply the energy requirement for foetal growth and subsequent lactation. The objective of this study was to evaluate the use of calcium salts of fatty acids (CSFA) to increase dietary energy level of peripartum dairy goats and its effects on nutritive value of diets and milk quality. Twenty multiparous Saanen goats (body weight 63.5±10.3 kg) were distributed in a completely randomised design in four treatments at the following dietary energy levels: 2.6 Mcal of metabolisable energy per kg of dry matter (Mcal ME/kg DM) - control diet; and 2.7, 2.8, and 2.9 Mcal/kg DM - with added CSFA. Goats were housed in individual stalls and evaluated in the peripartum period, by measuring body weight (BW), DMI, dry matter and nutrient digestibility of diets, blood composition, and milk quality and yield. Increasing dietary energy level to 2.9 Mcal ME/kg DM had no effect on BW and did not limit DMI. CSFA supplementation increased intake of total digestible nutrients and did not affect fibre digestion. Blood triglycerides and cholesterol concentration increased with CSFA addition. The treatments had no effect on milk yield and composition; however, CSFA supplementation changed the fatty acid concentration of milk fat, increasing levels of polyunsaturated and essential fatty acids. In conclusion, peripartum dairy goats supplemented with CSFA to increase dietary energy level up to 2.9 Mcal ME/kg DM had a greater supply of total digestible nutrients and showed altered fatty acid concentration of milk fat.

Control of Dietary Energy Level and dl-alpha-Tocopheryl Acetate Treatment Can Improve the Lipid Composition of Lamb Meat

2003 ◽  
Vol 73 (3) ◽  
pp. 171-179 ◽  
Author(s):  
Salvatori ◽  
Maiorano ◽  
Pantaleo ◽  
Brienza ◽  
Filetti ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Vitamin E ◽  
Energy Level ◽  
Shelf Life ◽  
Low Energy ◽  
Tocopheryl Acetate ◽  
Dietary Energy ◽  
Lamb Meat ◽  
Total Fat

A diet rich in saturated fatty acids promotes plasmatic cholesterol levels and coronary disease in humans, whereas a high intake of polyunsaturated fatty acids reduces atheromatous plaque thickness. This study aimed at establishing a dietary energy level, which combined with intramuscular vitamin E treatment, would improve the nutritional lipid quality and shelf-life of lamb meat. Twenty male lambs were evaluated in a 2 × 2 factorial experiment: they were fed a low- and normal-energy diet (0.85 and 1.00 UFV NE/kg DM, respectively), and were injected intramuscularly with 0 and 150 IU dl-alpha-tocopheryl acetate/weekly for eight weeks. Thereafter, total fat, cholesterol, fatty acid profile, and lipostability were measured in meat samples. Meat total fat was significantly reduced by low energy intake diet and vitamin E administration. Cholesterol was significantly lower in meat from lambs fed the 0.85 UFV NE/kg DM diet. Vitamin E treatment increased linoleic acid percent values and decreased myristic acid levels. Moreover, linoleic acid percentage was inversely correlated with muscle total fat concentration. Meat sensitivity to lipoperoxidation was inversely correlated with muscle vitamin E concentration. This study demonstrates that nutritional characteristics and shelf-life of meat benefit from a low-energy diet and intramuscular vitamin E treatment.

scholarly journals Alterations in Rumen Bacterial Community and Metabolome Characteristics of Cashmere Goats in Response to Dietary Nutrient Density

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1193
Author(s):  
Yaoyue Wang ◽  
Peng Tang ◽  
Yafei Xiao ◽  
Jianming Liu ◽  
Yulin Chen ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Metabolic Pathways ◽  
High Energy ◽  
High Protein ◽  
Metabolic Energy ◽  
Control Group ◽  
Rrna Gene ◽  
Dietary Energy ◽  
Nutrient Density ◽  
Cashmere Goats

This study was conducted to investigate the impacts of dietary energy and protein on rumen bacterial composition and ruminal metabolites. A total of 12 ruminal samples were collected from Shaanbei white cashmere goats which were divided into two groups, including high-energy and high-protein (Group H; crude protein, CP: 9.37% in dry matter; metabolic energy, ME: 9.24 MJ/kg) and control (Group C; CP: 8.73%; ME: 8.60 MJ/kg) groups. Thereby, 16S rRNA gene sequencing and a quantitative polymerase chain reaction were performed to identify the rumen bacterial community. Metabolomics analysis was done to investigate the rumen metabolites and the related metabolic pathways in Groups C and H. The high-energy and high-protein diets increased the relative abundance of phylum Bacteroidetes and genera Prevotella_1 and Succiniclasticum, while decreasing the number of Proteobacteria (p < 0.05). The dominant differential metabolites were amino acids, peptides, and analogs. Tyrosine metabolism played an important role among the nine main metabolic pathways. Correlation analysis revealed that both Prevotella_1 (r = 0.608, p < 0.05) and Ruminococcus_2 (r = 0.613, p < 0.05) showed a positive correlation with catechol. Our findings revealed that the diets with high energy and protein levels in Group H significantly altered the composition of ruminal bacteria and metabolites, which can help to improve the dietary energy and protein use efficiency in goats.

Effect of postweaning implantation of zeranol and dietary energy level on growth and reproductive performance of replacement beef heifers

1991 ◽  
Vol 71 (2) ◽  
pp. 265-270 ◽  
Author(s):  
M. Makarechian ◽  
P. F. Arthur ◽  
M. A. Price
Keyword(s):  
Reproductive Performance ◽  
Energy Requirement ◽  
Energy Levels ◽  
Reproductive Traits ◽  
High Energy ◽  
Dietary Energy ◽  
Maintenance Energy Requirement ◽  
And Control ◽  
Calving Difficulty ◽  
Effect On Growth

Eighty-one heifer calves weaned at an average age of 6 mo were used to study the effect of postweaning zeranol implantation, on the growth and reproductive performance of heifers fed to grow slowly on three dietary energy levels. Forty heifers were implanted with 36 mg of zeranol at 30 d and again at 120 d postweaning. The remaining heifers were used as ummplanted controls. Within the implanted and control groups, heifers were subdivided into three groups and fed either a low, medium or high energy diet, which provided approximately 105, 130 and 160%, respectively, of NRC maintenance energy requirement, until 1 yr of age. The heifers were then turned out to pasture with hay supplementation. Heifers were exposed to bulls for 35 d at approximately 14 mo of age. Zeranol had no significant effect on growth. The effect of zeranol on the reproductive traits studied was not significant, although the trend was that the zeranol implanted heifers had lower calf crop born (52.3 vs. 63.6%) and lower incidence of calving difficulty (18.5 vs. 32.1%) than control heifers. The level of energy in the diet had a significant (P < 0.01) effect on growth of the heifers, with those on the high energy diet having the highest mean growth rate, but no significant effect on reproductive performance. Key words: Zeranol, dietary energy, growth, reproduction, heifers

scholarly journals Performance and hormonal profile in broiler chickens fed with different energy levels during post restriction period

2003 ◽  
Vol 38 (6) ◽  
pp. 697-702 ◽  
Author(s):  
Poliana Fernanda Giachetto ◽  
Erika Nomura Guerreiro ◽  
Jesus Aparecido Ferro ◽  
Maria Inês Tiraboschi Ferro ◽  
Renato Luis Furlan ◽  
...  
Keyword(s):  
Energy Level ◽  
Broiler Chickens ◽  
Energy Levels ◽  
High Energy ◽  
Feed Restriction ◽  
Feed Conversion ◽  
Feeding Program ◽  
Ad Libitum ◽  
Ad Libitum Intake ◽  
Metabolic Hormone

The aim of this work was to investigate the influence of diet energy level on performance and hormonal profiles of broilers during post restriction period. It was a split-plot experiment, and the main treatments were in a 2x2 factorial scheme. Birds were fed restricted to 30% of the ad libitum intake, from 7 to 14 days of age. After the restriction period, birds were fed ad libitum with diets containing low (2,900 kcal ME/kg) or high (3,200 kcal ME/kg) energy until 49 days of age. Broilers fed with high energy ration showed lower feed intake and better feed conversion and decreased carcass protein; however, abdominal fat pad, and total carcass fat were not affected by ration energy levels or feeding program. Neither diet energy level nor feed restriction program changed body weight at 49 days. The profile of insulin-like growth factor-1 (IGF-1) was reduced during the feed restriction period, but increased at refeeding period. Feeding program and ration energy level did not affect T3, T4 and growth hormone serum concentrations. Feed restriction at 30% of ad libitum intake is not enough to promote changes on carcass quality, related to fat deposition, and on metabolic hormone levels, except IGF-1 seric level that has rapid increase after feed restriction.

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