scholarly journals Feed Preference Response of Weaner Bull Calves to Bacillus amyloliquefaciens H57 Probiotic and Associated Volatile Organic Compounds in High Concentrate Feed Pellets

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 51
Author(s):  
Thi Thuy Ngo ◽  
Nguyen N. Bang ◽  
Peter Dart ◽  
Matthew Callaghan ◽  
Athol Klieve ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Bacillus Amyloliquefaciens ◽  
Equal Access ◽  
Relative Preference ◽  
Bull Calves ◽  
Volatile Organic ◽  
Feed Pellets ◽  
Microbial Volatile Organic Compounds ◽  
Odour Profile

This study tested the hypothesis that Bacillus amyloliquefaciens strain H57 (H57) improves preference by reducing the development of microbial volatile organic compounds (mVOCs) in feed pellets. Sixteen bull calves were, for 4 weeks, provided equal access to a panel of 8 automated feed bunks in a single paddock with some hay. Each bunk contained pellets with (H57) or without (Control) the H57, each aged for 4 months at either ambient or chiller temperature. Each treatment was changed to a new bunk pair position weekly. Relative preference was determined according to weight of pellets remaining per hour per treatment bunk pair per 24 h. Pellets were analysed for volatile organic compounds (VOCs) and the concentrations tested for correlation with relative preference. Calves showed the lowest preference (p < 0.0001) for the Control/Ambient treatment whereas preference for all other treatments (H57/Ambient; H57/Chiller; Control/Chiller) was similar. The Control/Ambient treatment odour profile grouped differently to the other 3 treatments which grouped similarly to each other. Up to 16 mVOCs were determined to have potential as pre-ingestive signals for the extent of microbial spoilage. Further studies are required to find which combination of these mVOCs, when added to pellets, results in feed aversion.

scholarly journals Volatile Organic Compound Profiles Associated with Microbial Development in Feedlot Pellets Inoculated with Bacillus amyloliquefaciens H57 Probiotic

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3227
Author(s):  
Thi Thuy Ngo ◽  
Peter Dart ◽  
Matthew Callaghan ◽  
Athol Klieve ◽  
David McNeill
Keyword(s):  
Volatile Organic Compounds ◽  
Ambient Temperature ◽  
Organic Compounds ◽  
Bacillus Amyloliquefaciens ◽  
Principal Component ◽  
Gas Chromatography Mass Spectrometry ◽  
Score Plot ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds ◽  
Odour Profile

Mould and bacterial contamination releases microbial volatile organic compounds (mVOCs), causing changes in the odour profile of a feed. Bacillus amyloliquefaciens strain H57 (H57) has the potential ability to inhibit microbial growth in animal feeds. This study tested the hypothesis that H57 influences the odour profile of stored feedlot pellets by impeding the production of mVOCs. The emission of volatile organic compounds (VOCs) of un-inoculated pellets and those inoculated with H57, stored either at ambient temperature (mean 22 °C) or at 5 °C, was monitored at 0, 1, 2, and 3 months by gas chromatography–mass spectrometry. Forty VOCs were identified in all the pellet samples analysed, 24 of which were potentially of microbial and 16 of non-microbial origin. A score plot of the principal component analysis (PCA) showed that the VOC profiles of the pellets stored at ambient temperature changed more rapidly over the 3 months than those stored at 5 °C, and that change was greater in the un-inoculated pellets when compared to the inoculated ones. The bi-plot and correlation loading plots of the PCA indicated that the separation of the un-inoculated pellets from the other treatments over the 3 months was primarily due to nine mVOCs. These mVOCs have been previously identified in grains spoiled by fungi, and could be considered potential markers of the types of fungi that H57 can protect pellets against. These data indicate the ability of H57 to maintain the odour profile and freshness of concentrated feed pellets. This protective influence can be detected as early as 3 months into ambient temperature storage.

Variable emissions of microbial volatile organic compounds (MVOCs) from root-associated fungi isolated from Scots pine

2010 ◽  
Vol 44 (30) ◽  
pp. 3651-3659 ◽  
Author(s):  
Jaana Bäck ◽  
Hermanni Aaltonen ◽  
Heidi Hellén ◽  
Maija K. Kajos ◽  
Johanna Patokoski ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Scots Pine ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds

Detection and purification of microbial volatile organic compounds

2021 ◽  
pp. 51-64
Author(s):  
Jeevanandam Vaishnavi ◽  
Swarnkumar Reddy ◽  
Santhanam Narmadha ◽  
W. Jabez Osborne
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds

Pathogen suppression by microbial volatile organic compounds in soils

2019 ◽  
Vol 95 (8) ◽  
Author(s):  
Wietse de Boer ◽  
Xiaogang Li ◽  
Annelein Meisner ◽  
Paolina Garbeva
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Abiotic Factors ◽  
Management Strategies ◽  
Field Studies ◽  
Growth Media ◽  
Soil Borne Pathogens ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds ◽  
Pathogen Suppression

ABSTRACT There is increasing evidence that microbial volatile organic compounds (mVOCs) play an important role in interactions between microbes in soils. In this minireview, we zoom in on the possible role of mVOCs in the suppression of plant-pathogenic soil fungi. In particular, we have screened the literature to see what the actual evidence is that mVOCs in soil atmospheres can contribute to pathogen suppression. Furthermore, we discuss biotic and abiotic factors that influence the production of suppressive mVOCs in soils. Since microbes producing mVOCs in soils are part of microbial communities, community ecological aspects such as diversity and assembly play an important role in the composition of produced mVOC blends. These aspects have not received much attention so far. In addition, the fluctuating abiotic conditions in soils, such as changing moisture contents, influence mVOC production and activity. The biotic and abiotic complexity of the soil environment hampers the extrapolation of the production and suppressing activity of mVOCs by microbial isolates on artificial growth media. Yet, several pathogen suppressive mVOCs produced by pure cultures do also occur in soil atmospheres. Therefore, an integration of lab and field studies on the production of mVOCs is needed to understand and predict the composition and dynamics of mVOCs in soil atmospheres. This knowledge, together with the knowledge of the chemistry and physical behaviour of mVOCs in soils, forms the basis for the development of sustainable management strategies to enhance the natural control of soil-borne pathogens with mVOCs. Possibilities for the mVOC-based control of soil-borne pathogens are discussed.

scholarly journals Detection and Identification of Microbial Volatile Organic Compounds of the Green Mold Disease

2020 ◽  
Vol 11 (2) ◽  
pp. 14-28
Author(s):  
Dalma Radványi ◽  
András Geösel ◽  
Zsuzsa Jókai ◽  
Péter Fodor ◽  
Attila Gere
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Identification System ◽  
Mushroom Species ◽  
Detection And Identification ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds ◽  
Green Mould

Button mushrooms are one of the most commonly cultivated mushroom species facing different risks e.g.: viral, bacterial and fungal diseases. One of the most common problems is caused by Trichoderma aggressivum, or ‘green mould' disease. The presence or absence of mushroom disease-related moulds can sufficiently be detected from the air by headspace solid-phase microextraction coupled gas chromatography-mass spectrometry (HS SPME GC-MS) via their emitted microbial volatile organic compounds (MVOCs). In the present study, HS SPME GC-MS was used to explore the volatile secondary metabolites released by T. aggressivum f. europaeum on different nutrient-rich and -poor media. The MVOC pattern of green mould was determined, then media-dependent and independent biomarkers were also identified during metabolomic experiments. The presented results provide the basics of a green mould identification system which helps producers reducing yield loss, new directions for researchers in mapping the metabolomic pathways of T. aggressivum and new tools for policy makers in mushroom quality control.

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