scholarly journals Effect of Dietary Rumen-Protected L-Tryptophan Supplementation on Growth Performance, Blood Hematological and Biochemical Profiles, and Gene Expression in Korean Native Steers under Cold Environment

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1036 ◽  
Author(s):  
Jae-Sung Lee ◽  
Wahyu Priatno ◽  
Jalil Ghassemi Nejad ◽  
Dong-Qiao Peng ◽  
Jin-Seung Park ◽  
...  

We assessed the growth performance, physiological traits, and gene expressions in steers fed with dietary rumen-protected L-tryptophan (RPT) under a cold environment. Eight Korean native steers were assigned to two dietary groups, no RPT (Control) and RPT (0.1% RPT supplementation on a dry matter basis) for six weeks. Maximum and minimum temperatures throughout the experiment were 6.7 °C and −7.0 °C, respectively. Supplementation of 0.1% RPT to a total mixed ration did not increase body weight but had positive effects of elevating average daily gain (ADG) and reducing the feed conversion ratio (FCR) on days 27 and 48. The metabolic parameter showed a higher glucose level (on day 27) in the 0.1% RPT group compared to the control group. Real-time PCR analysis showed no significant differences in the expression of muscle (MYF6, MyoD, and Desmin) metabolism genes between the two groups, whereas the expression of fat (PPARγ, C/EBPα, and FABP4) metabolism genes was lower in the 0.1% RPT group than in the control group. Thus, we demonstrate that long-term (six weeks) dietary supplementation of 0.1% RPT was beneficial owing to enhanced growth performance by increasing the ADG and glucose level, decreasing FCR, and maintaining homeostasis in immune responses in beef steers in a cold environment.

2013 ◽  
Vol 16 (4) ◽  
pp. 619-627 ◽  
Author(s):  
M. Pogány Simonová ◽  
A. Lauková ◽  
L. Chrastinová ◽  
I. Plachá ◽  
V. Strompfová ◽  
...  

Abstract The effect of Enterococcus faecium CCM7420 (EF) - enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.


Author(s):  
Amr M.A. Elmasry ◽  
Luis A. Miranda ◽  
Germán Mendoza ◽  
Fernando X. Plata

Background: The effects of the probiotics vary with the doses and strains used for this reason, a study was conducted over 35 days to determine the effect of live yeast supplementation in the diet of growing rabbits on growth performance and apparent nutrient digestibility.Methods: A total of 60 New Zealand and California rabbits (5 weeks old, average body weight 0.881±0.015 kg) were housed in 30 cages. They were fed with a control diet based on alfalfa and maize or barley grains and supplemented with Procreatin®7 (Saccharomyces cerevisiae CNCM I 1077) or Biosaf® SC47 (Saccharomyces cerevisiae NCYC Sc47) at three doses, namely 0, 6.4, or 12.8 ×109 CFU per kg basal diet.Result: The higher yeast concentration had a positive effect (P less than 0.01) on final body weight (BW), average daily gain (ADG) and carcass weight (CW). However, there were no significant differences in dry matter intake. As a result of this, the feed conversion ratio (FCR) decreased (P less than 0.01). Significant increases (P less than 0.05) in nutrient digestibility (DM, OM, NDF and ADF) were observed with both grains. The use of yeast in maize and barley-based diets of rabbits has positive effects on growth performance and nutrient digestibility.


2021 ◽  
Vol 14 (4) ◽  
pp. 878-883
Author(s):  
Fatma Boubekeur ◽  
Rafik Arbouche ◽  
Yasmine Arbouche ◽  
Fodil Arbouche

Background and Aim: The rearing of quails can have a stronger attraction for the breeders if we lower the cost prices by introducing by-products in their feed formulas. The aim of this study was to evaluate the effects of the partial substitution of soybean meal by apricot kernel cake (AKC) in the diet of quails, applied either sequentially or during all phases of rearing, on their growth performances, carcass characteristics, and meat physicochemical composition. Materials and Methods: A total of 600 one-day-old quails (Coturnix coturnix japonica), with equal sex ratio and weighing on average 7±0.2 g, were randomly distributed in one control group and three experimental groups, the latter being designed according to the rate of application of AKC in either sequential or non-sequential mode in different rearing phases. Each group was divided into five replicates of 30 quails, randomly distributed according to either substitution rate of soybean meal by the AKC (0%, 10%, 20%, or 30%) or farming phase. Results: The average daily gain from the 1st to 45th days (average daily gain1–45d) was found to be the highest (4.24 g/d/subject, p=0.021) for the 30% AKC-supplemented feed lot in either starter or finish incorporation (DFTAA), having an optimum final live weight of 193.4 g (p=0.028), a lowest feed conversion ratio of 3.08 (p=0.001), and a daily feed intake of 860 g (p=0.01). Carcass yield was recorded the highest (74.4%, p=0.02) with an optimum meat protein level (30.6%, p=0.024) and the lowest fat content (2.26%, p=0.001) for the same group as well. Conclusion: The partial substitution of soybean meal by AKC in the quails' fattening feeding, during the finishing phase and for all rearing phases, led to a better growth performance, a better carcass yield, and an improved chemical composition of meat.


Author(s):  
N. Zhan ◽  
H. Li ◽  
C. Jiang ◽  
Y. Tu ◽  
D. Diao

To investigate the effect of lipopolysaccharides (LPS) on the growth performance, nitrogen metabolism and immunity in preruminant calves, forty Chinese Holstein male calves were randomly divided into two groups and injected intraperitoneally with 2.5 mg/kg BW of sterile saline or E. coli LPS (Escherichia coli O55:b55) on day 24, 26, and 28. The LPS challenge decreased average daily gain and feed conversion efficiency compared with control group (p less than0.05). The urinary concentration of nitrogen (pless than0.05) increased, whereas the nitrogen retention decreased (pless than0.05) after LPS-challenge. Injection of LPS increased body temperature, serum concentration of IL-1, TNF-a, IL-2 and IgG of calves. In conclusion, LPS-challenge activated immune responses, decreased utilization efficiency of nitrogen, and negatively impacted growth performance of preruminant calves.


Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2430
Author(s):  
Byung-Ki Park ◽  
Jun-Sang Ahn ◽  
Min-Ji Kim ◽  
Gi-Hwal Son ◽  
Sang-Hun Bong ◽  
...  

This study was conducted to investigate the effects of rumen-protected methionine and α-tocopherol (RPMT) on growth performance, carcass characteristics, and meat composition of late fattening Hanwoo steers in high-temperature seasons. Fourteen steers were randomly assigned to one of two diets; control (commercial concentrate) and treatment (commercial concentrate + 20 g of RPMT). Average daily gain was 34% higher in the treatment group than in the control group; however, there was no significant difference due to the small number under investigation. Feed conversion ratio was lower in the treatment group than in the control group (p < 0.05). Supplementation of RPMT had little effect on the fatty acid composition of longissimus muscle. Metmyoglobin in the longissimus muscle was significantly lower in treatment group compared to the control group at the ninth day of storage (p < 0.05). The redness of the longissimus muscle was higher in the treatment group than in the control group on day 9 of storage (p < 0.01). Thus, the results suggest that RPMT have positive effects on growth performance, and Commission Internationale de l’Eclairage color stability in the longissimus muscle of late fattening Hanwoo steers in high-temperature seasons.


2020 ◽  
Author(s):  
Hang Zhao ◽  
Saikun Pan

Abstract Background: The present study aimed to explore the effects of dietary chitosan oligosaccharides (COs) on the growth performance and immunity of broiler chickens.Methods: Four test diets were supplemented with different doses of COs (0, 50, 100 and 150 mg/kg) and formulated. Triplicate groups of broiler chickens were fed with one of the diets ad libitum for 6 weeks. Results: After the end of the feeding trials, oral CO administration improved average daily gain; the activities of digestive amylase, lipase and protease; the activities of serum superoxide dismutase and glutathione peroxidase; serum tumor necrosis factor-α, interleukin (IL)-1, IL-2, IgG, IgM and IgA levels; and reduced feed conversion ratio and malondialdehyde level compared with those of the control group. However, a high dose of CO (150 g/kg) did not further increase its efficiency compared with the moderate dose of CO (100 g/kg). Conclusions: Oral CO administration improved the growth performance and immunity of juvenile broiler chickens and could be used as a feed supplement.


Author(s):  
C.W. Wang ◽  
Y. Jiang ◽  
W.N. Chen ◽  
Q. Liu ◽  
X.F. Cheng ◽  
...  

Background: This study was conducted to evaluate the effect of dietary LJP supplementation on growth performance, heat stress resistance and immune status of weaned piglets raised in a high-temperature environment (34 ± 3°C).Methods: A total of 72 Barkshire × Licha Black crossbred piglets were weaned at 30 days of age (BW, 5.88 ± 0.16 kg) and fed either without or with 100, 200 or 400 mg/kg LJP in a completely randomized design, with 3 pens per diet (6 pigs per pen). On day 21, a total of 12 piglets were selected to collect blood samples.Result: The results showed that 200 and 400 mg/kg LJP supplementation decreased the feed conversion ratio (FCR) of piglets compared to the control group (P = 0.037) and dietary LJP linearly increased average daily gain and decreased FCR of piglets (P = 0.037 and 0.011, respectively). In addition, adding 100, 200 and 400 mg/kg LJP reduced the serum HSP70 content compared to the control group (P = 0.019) and dietary LJP linearly and quadratically decreased the serum HSP70 content of piglets (P = 0.030 and 0.011, respectively). Moreover, supplementation of 200 mg/kg and 400 mg/kg LJP increased the serum (NO) and nitric oxide acid phosphatase (ACP) content compared to the control group (P = 0.019; P = 0.003) and dietary LJP linearly increased the serum lysozyme, NO and ACP contents (P = 0.039; P = 0.005; P = 0.001). In conclusion, dietary LJP has potential to regulate systemic defence property of weaned piglets raised in a high temperature condition, accompanied by increased growth performance.


Author(s):  
M. Punyatong ◽  
W. Tapingkae ◽  
N. Pripwai ◽  
W. Laenoi

This study investigated the effect of purple glutinous rice bran (PRB) supplementation on performance, oxidative status and lipid oxidation in broiler. Two hundred male day old broiler chickens were randomly assigned to 5 groups of 40 birds each. Each treatment, fed the basal corn-soybean diets containing 0%, 3%, 6%, 9% and 12% of purple glutinous rice bran (PRB). Diets were formulated separately for starter and finisher phases (day 0 to 21 and day 22 to 42). Oxygen radical absorbance capacity (ORAC) in PRB supplemented groups were higher than rice bran group and depend on the supplement dose of PRB. PRB supplementation (6%, 9% and 12%) increased (P less than 0.05) weight gain (WG), average daily gain (ADG) and decreased (p less than 0.05) feed conversion ratio (FCR) compared to control group. Malondialdehyde (MDA) levels in serum from the PRB groups were lower than control group (P less than 0.05). Glutathione (GSH) levels in serum of PRB groups (6%, 9% and 12% PRB) were higher than control group (P less than 0.05). However, there were no difference between 3% PRB and control groups. No difference of lipid oxidation in breast meat after storage between PRB supplemented groups with control group (P>0.05). This study indicated that PRB supplementation in diet had positive effects on performance and oxidative status of broiler chickens but had no effect on lipid oxidation in breast meat storage


Author(s):  
Wen-Chao Liu ◽  
Yan Guo ◽  
Zhi-Hui Zhao ◽  
Rajesh Jha ◽  
Balamuralikrishnan Balasubramanian

This study aimed to determine the efficacy of dietary algae-derived polysaccharides (ADPs) from Enteromorpha on growth performance, intestinal morphology, intestinal permeability, and antioxidant capacity in serum, liver, and intestinal mucosa of broilers. Three hundred and ninety six day-old male chicks were randomly assigned to six dietary treatments containing 0 (Control), 1,000, 2,500, 4,000, 5,500, and 7,000 mg ADP/kg basal diet in a 35 day feeding trial. During day 1–21, compared with the control group, dietary 1,000–7,000 mg/kg ADP supplementation improved the average daily gain (ADG) and feed conversion ratio (p &lt; 0.05). Overall (day 1–35), dietary inclusion of 1,000 mg/kg ADP increased the final body weight and ADG (p &lt; 0.05). Besides, on day 21, dietary 2,500 mg/kg ADP supplementation increased the serum catalase (CAT) and liver total superoxide dismutase (T-SOD) activities (p &lt; 0.05), whereas dietary 1,000–5,500 mg/kg ADP supplementation decreased malondialdehyde (MDA) contents in serum and liver (p &lt; 0.05). On day 35, supplementation of 1,000 mg/kg ADP increased the serum glutathione peroxidase and CAT activities and liver T-SOD activities (p &lt; 0.05). It decreased the MDA level of serum and liver (p &lt; 0.05). Also, dietary 2,500 mg/kg ADP increased the villus height of jejunum and ileum on day 21 (p &lt; 0.05), and dietary 4,000 mg/kg ADP increased the villus height of duodenum and ileum on day 35 (p &lt; 0.05). On day 21, dietary 4,000 mg/kg ADP increased the CAT activities of the duodenum and T-SOD activities of jejunum and ileum and decreased the MDA contents in the duodenum, jejunum, and ileum (p &lt; 0.05). On day 35, dietary inclusion of 1,000–7,000 mg/kg ADP reduced MDA contents of duodenum and jejunum (p &lt; 0.05). Furthermore, dietary inclusion of ADP at 1,000–7,000 mg/kg decreased serum DAO activities at day 21 and day 35 (p &lt; 0.05), and the serum D-lactic acid concentration was reduced by dietary supplementation of 1,000, 2,500, and 7,000 mg/kg ADP on day 21. In conclusion, dietary ADP exerted beneficial effects on growth performance, antioxidant capacity, and gut health in broilers; based on the studied parameters, the appropriate recommended dose is 1,000–4,000 mg/kg. These findings provided new insights into the potential application of ADP as natural growth promoters in broilers.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 170-171
Author(s):  
Laura A Merriman ◽  
Gustavo Cordero ◽  
Pete Wilcock ◽  
Joaquin Morales

Abstract As an alternative to antibiotic growth promoters, live yeast supplementation has proven useful in reducing weaning stress and improving health and performance of piglets. The objective of the study was to evaluate the effect of a high concentrated live yeast product (20 billion CFU/g) supplemented in post-weaning piglet diets without antibiotics. A total of 144 pigs Pietrain × [LW×Landrace] (average initial BW = 8.55 ± 1.5 kg) weaned at 28 days of life were used for a 6-week growth study to determine post-weaning performance. Pigs were assigned to 2 treatments; 0 (Control group; CT) and 1 g of live yeast/kg (Yeast Supplemented group; YS) feed to 12 replicate pens of 6 pigs (3 barrows; 3 gilts). Average daily gain (ADG), feed intake (ADFI) and feed conversion ratio (FCR) were evaluated in pre-starter (days 0–14), starter (days 14–42) and in the whole nursery (days 0–42) periods. Data were analysed with ANOVA using PROC MIXED of SAS according to the completely randomized design. The model included initial BW as a covariant. Differences were deemed statistically significant when P ≤ 0.050 using a one tailed t-test. Live yeast supplementation improved ADG in starter (5.8%; P = 0.034) and in the whole nursery periods (7.0%; P = 0.045) compared with the CT group. Consequently, final BW was also higher in supplemented animals than in the CT (4.5%; P = 0.034). Differences in ADG were associated with differences in ADFI, higher in YS than in the CT group, both in starter (6.4%; P = 0.023) and in the whole nursery period (6.5%; P = 0.030). No differences were observed for FCR, mortality rate, and health incidences between dietary treatment groups. Therefore, supplementation of a live yeast to post-weaned piglets improved growth performance, confirming this product as an alternative to reduce antibiotic usage in nursery piglets.


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