scholarly journals Development of a Ratiometric Fluorescent Glucose Sensor Using an Oxygen-Sensing Membrane Immobilized with Glucose Oxidase for the Detection of Glucose in Tears

Biosensors ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 86 ◽  
Author(s):  
Hong Dinh Duong ◽  
Ok-Jae Sohn ◽  
Jong Il Rhee
Keyword(s):  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Glucose Sensor ◽  
Oxygen Sensing ◽  
Sol Gel ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Detection Range ◽  
Fluorescence Measurements ◽  
Sensing Membrane

Glucose concentration is an important parameter in biomedicine since glucose is involved in many metabolic pathways in organisms. Many methods for glucose detection have been developed for use in various applications, particularly in the field of healthcare in diabetics. In this study, ratiometric fluorescent glucose-sensing membranes were fabricated based on the oxygen levels consumed in the glucose oxidation reaction under the catalysis of glucose oxidase (GOD). The oxygen concentration was measured through the fluorescence quenching effect of an oxygen-sensitive fluorescent dye like platinum meso-tetra (pentafluorophenyl) porphyrin (PtP) by oxygen molecules. Coumarin 6 (C6) was used as a reference dye in the ratiometric fluorescence measurements. The glucose-sensing membrane consisted of two layers: The first layer was the oxygen-sensing membrane containing polystyrene particles (PS) doped with PtP and C6 (e.g., PS@C6^PtP) in a sol–gel matrix of aminopropyltrimethoxysilane and glycidoxypropyltrimethoxysilane (GA). The second layer was made by immobilizing GOD onto one of three supporting polymers over the first layer. These glucose-sensing membranes were characterized in terms of their response, reversibility, interferences, and stability. They showed a wide detection range to glucose concentration in the range of 0.1 to 10 mM, but high sensitivity with a linear detection range of 0.1 to 2 mM glucose. This stable and sensitive ratiometric fluorescent glucose biosensor provides a reliable way to determine low glucose concentrations in blood serum by measuring tear glucose.

scholarly journals Ratiometric Fluorescent Biosensors for Glucose and Lactate Using an Oxygen-Sensing Membrane

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 208
Author(s):  
Hong Dinh Duong ◽  
Jong Il Rhee
Keyword(s):  
Limit Of Detection ◽  
Oxygen Sensing ◽  
High Sensitivity ◽  
Glucose Sensing ◽  
Oxidation Reactions ◽  
Lactate Oxidase ◽  
Lactate Oxidation ◽  
Detection Range ◽  
Quenching Effect ◽  
Sensing Membrane

In this study, ratiometric fluorescent glucose and lactate biosensors were developed using a ratiometric fluorescent oxygen-sensing membrane immobilized with glucose oxidase (GOD) or lactate oxidase (LOX). Herein, the ratiometric fluorescent oxygen-sensing membrane was fabricated with the ratio of two emission wavelengths of platinum meso-tetra (pentafluorophenyl) porphyrin (PtP) doped in polystyrene particles and coumarin 6 (C6) captured into silica particles. The operation mechanism of the sensing membranes was based on (i) the fluorescence quenching effect of the PtP dye by oxygen molecules, and (ii) the consumption of oxygen levels in the glucose or lactate oxidation reactions under the catalysis of GOD or LOX. The ratiometric fluorescent glucose-sensing membrane showed high sensitivity to glucose in the range of 0.1–2 mM, with a limit of detection (LOD) of 0.031 mM, whereas the ratiometric fluorescent lactate-sensing membrane showed the linear detection range of 0.1–0.8 mM, with an LOD of 0.06 mM. These sensing membranes also showed good selectivity, fast reversibility, and stability over long-term use. They were applied to detect glucose and lactate in artificial human serum, and they provided reliable measurement results.

scholarly journals Glucose biosensor based on entrapment of glucose oxidase and myoglobin in silica gel by the sol-gel method

2005 ◽  
Vol 19 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Mohammed A. Zaitoun
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Decay Curve ◽  
Sol Gel ◽  
Porous Silica ◽  
Glucose Biosensor ◽  
Negative Peak ◽  
Before And After ◽  
Porous Silica Glass

A spectrophotometric method is presented to determine glucose employing the sol-gel technique. Myoglobin (Mb) and glucose oxidase are encapsulated in a transparent and porous silica glass. The produced gel (xerogel) is then immersed in water where increments of glucose are added to the solution with stirring; glucose diffuses into the sol-gel glass pores and a series of reactions take place. Glucose is first oxidized by glucose oxidase and oxygen to gluconate and hydrogen peroxide is generated. The liberated hydrogen peroxide oxidizes the Mb heme (Fe2+into Fe3+). The higher is the glucose concentration added, the more is the H2O2generated, and the more is the Mb oxidation (Fe2+to Fe3+) and as a result the higher is the absorbance at 400 nm (negative peak, lower absorbance value). All measurements are performed at this wavelength (400 nm), the negative peak obtained by subtracting the absorption spectra of Mb before and after oxidation. Measuring the slope of the absorbance decay versus time at 400 nm monitors increments of added glucose. Each glucose concentration has an accompanying unique decay curve with a unique slope. The higher is the glucose concentration; the steeper is the decay curve (higher slope value). The calibration curve was linear up to 40 mM.

Glucose concentration determination based on silica sol–gel encapsulated glucose oxidase optical biosensor arrays

Talanta ◽  
2010 ◽  
Vol 83 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Gang Chang ◽  
Yoshiro Tatsu ◽  
Tatsushi Goto ◽  
Hiromasa Imaishi ◽  
Kenichi Morigaki
Keyword(s):  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Sol Gel ◽  
Optical Biosensor ◽  
Silica Sol ◽  
Biosensor Arrays ◽  
Concentration Determination

scholarly journals Reduced graphene oxide-supported methylene blue nanocomposite as a glucose oxidase-mimetic for electrochemical glucose sensing

RSC Advances ◽  
2018 ◽  
Vol 8 (57) ◽  
pp. 32565-32573 ◽  
Author(s):  
Shaojun Yang ◽  
Daliang Liu ◽  
Qing Bo Meng ◽  
Shuyao Wu ◽  
Xi-Ming Song
Keyword(s):  
Methylene Blue ◽  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Electrocatalytic Activity ◽  
Glucose Sensor ◽  
Glucose Sensing ◽  
Reduced Graphene

A novel electrochemical glucose sensor based on methylene blue-reduced graphene oxide nanocomposite was constructed, and the sensor exhibited good glucose oxidase-mimetic electrocatalytic activity towards glucose and practical applicability.

scholarly journals Polyaniline nanoflowers grafted onto nanodiamonds via a soft template-guided secondary nucleation process for high-performance glucose sensing

RSC Advances ◽  
2017 ◽  
Vol 7 (25) ◽  
pp. 15342-15351 ◽  
Author(s):  
S. Komathi ◽  
A. I. Gopalan ◽  
N. Muthuchamy ◽  
K. P. Lee
Keyword(s):  
Glucose Oxidase ◽  
High Performance ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Soft Template ◽  
Nucleation Process ◽  
Secondary Nucleation

We demonstrated that the electrochemical glucose biosensor fabricated using glucose oxidase-immobilized polyaniline nanoflower-grafted nanodiamonds exhibits superior performances.

scholarly journals Preparation of an Amperometric Glucose Biosensor on Polyaniline-Coated Graphite

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Shova Neupane ◽  
Suresh Bhusal ◽  
Vivek Subedi ◽  
Krishna Badan Nakarmi ◽  
Dipak Kumar Gupta ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Glucose Concentration ◽  
Reduction Potential ◽  
Low Cost ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Pani Film ◽  
Current Response ◽  
Electrochemically Deposited ◽  
Effects Of Ph

Control of glucose concentration has tremendous significance in medical diagnosis, pharmaceuticals, food, and fermentation industries. Herein, we report on the fabrication of a facile, low-cost, and sensitive enzyme-based amperometric sensor using the electrochemically deposited polyaniline (PANI) film on a graphite electrode. PANI was deposited from an aqueous solution of 0.2 M aniline in 1.0 M hydrocholoric acid (HCl) by cyclic voltammetry (CV). Surface morphology and composition characterization of the PANI film were carried out by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Fourier-transform infrared (FTIR) spectroscopy. Potentiostatic immobilization of glucose oxidase (GOX) enzyme in the PANI film was carried out at 0.75 V to fabricate an amperometric glucose biosensor (GOx/PANI/graphite biosensor). The glucose concentration response of the prepared sensor was studied amperometrically by detecting hydrogen peroxide (H2O2). The detection of H2O2 was optimized by calibrating the effects of pH, reduction potential, and background current. A reduction potential of -0.4 V at pH 6 was the best combination to get a maximum amperometric response of the GOx/PANI/graphite biosensor. A stable current response was obtained in 4 min with a high reproducibility in linearity within the concentration range of 0.01 M-0.1 M D-glucose. Therefore, the fabricated GOx/PANI/graphite biosensor could be a promising candidate for glucose sensing.

Entrapment of Glucose Oxidase in Silica Gel by the Sol-Gel Method and Its Application to Glucose Sensor

1992 ◽  
Vol 21 (8) ◽  
pp. 1615-1618 ◽  
Author(s):  
Yoshiro Tatsu ◽  
Keishi Yamashita ◽  
Muneaki Yamaguchi ◽  
Soichiro Yamamura ◽  
Hitoshi Yamamoto ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Silica Gel ◽  
Glucose Sensor ◽  
Sol Gel ◽  
Gel Method ◽  
Sol Gel Method
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