scholarly journals Bioprinting on 3D Printed Titanium Scaffolds for Periodontal Ligament Regeneration

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1337
Author(s):  
Ui-Lyong Lee ◽  
Seokhwan Yun ◽  
Hua-Lian Cao ◽  
Geunseon Ahn ◽  
Jin-Hyung Shim ◽  
...  
Keyword(s):  
Dental Implants ◽  
Periodontal Ligament ◽  
Defense Mechanisms ◽  
Animal Experiments ◽  
Three Dimensional ◽  
Artificial Organs ◽  
Artificial Organ ◽  
Mechanical Stimuli ◽  
3D Printed

The three-dimensional (3D) cell-printing technique has been identified as a new biofabrication platform because of its ability to locate living cells in pre-defined spatial locations with scaffolds and various growth factors. Osseointegrated dental implants have been regarded as very reliable and have long-term reliability. However, host defense mechanisms against infections and micro-movements have been known to be impaired around a dental implant because of the lack of a periodontal ligament. In this study, we fabricated a hybrid artificial organ with a periodontal ligament on the surface of titanium using 3D printing technology. CEMP-1, a known cementogenic factor, was enhanced in vitro. In animal experiments, when the hybrid artificial organ was transplanted to the calvarial defect model, it was observed that the amount of connective tissue increased. 3D-printed hybrid artificial organs can be used with dental implants, establishing physiological tooth functions, including the ability to react to mechanical stimuli and the ability to resist infections.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

Functional Behaviour of Human Periodontal Ligament Cells around Various Dental Implants

2007 ◽  
Vol 361-363 ◽  
pp. 837-840 ◽  
Author(s):  
Xiao Ting Luo ◽  
Zhen Gao ◽  
Shi Gui Yan ◽  
Wei Deng ◽  
Wen Shu Zhang ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Dental Implants ◽  
Periodontal Ligament ◽  
Cell Counting ◽  
Immunofluorescent Staining ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells ◽  
Proliferation And Differentiation ◽  
In Vitro Systems

In the present investigation, four titanium (Ti) surfaces of dental implants were compared through in vitro systems. The surface roughness of Ti was measured by TR240 mobile surface roughmeter. The Ti implants were seeded with human periodontal ligament cells (hPLDCs) and maintained for a period of 0-7 days. The adhesion, proliferation, and differentiation of hPLDCs were observed by using Cell morphology, cell counting and Osteocalcin (OC) immunofluorescent staining. Results suggest that surface roughness of titanium favors hPDLCs behavior and improves cell adhesion, proliferation, and differentiation.

scholarly journals Evaluation of the 3D Printing Accuracy of a Dental Model According to Its Internal Structure and Cross-Arch Plate Design: An In Vitro Study

Materials ◽  
2020 ◽  
Vol 13 (23) ◽  
pp. 5433
Author(s):  
Seung-Ho Shin ◽  
Jung-Hwa Lim ◽  
You-Jung Kang ◽  
Jee-Hwan Kim ◽  
June-Sung Shim ◽  
...  
Keyword(s):  
3D Printing ◽  
Internal Structure ◽  
Three Dimensional ◽  
In Vitro Study ◽  
Thick Shell ◽  
Plate Design ◽  
Dental Model ◽  
3D Printed ◽  
Modeling Data

The amount of photopolymer material consumed during the three-dimensional (3D) printing of a dental model varies with the volume and internal structure of the modeling data. This study analyzed how the internal structure and the presence of a cross-arch plate influence the accuracy of a 3D printed dental model. The model was designed with a U-shaped arch and the palate removed (Group U) or a cross-arch plate attached to the palate area (Group P), and the internal structure was divided into five types. The trueness and precision were analyzed for accuracy comparisons of the 3D printed models. Two-way ANOVA of the trueness revealed that the accuracy was 135.2 ± 26.3 µm (mean ± SD) in Group U and 85.6 ± 13.1 µm in Group P. Regarding the internal structure, the accuracy was 143.1 ± 46.8 µm in the 1.5 mm-thick shell group, which improved to 111.1 ± 31.9 µm and 106.7 ± 26.3 µm in the roughly filled and fully filled models, respectively. The precision was 70.3 ± 19.1 µm in Group U and 65.0 ± 8.8 µm in Group P. The results of this study suggest that a cross-arch plate is necessary for the accurate production of a model using 3D printing regardless of its internal structure. In Group U, the error during the printing process was higher for the hollowed models.

scholarly journals An Implantable Sensorized Lead for Continuous Monitoring of Cardiac Apex Rotation

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4195
Author(s):  
Emanuela Marcelli ◽  
Laura Cercenelli
Keyword(s):  
Continuous Monitoring ◽  
Dimensional Space ◽  
Animal Experiments ◽  
Three Dimensional ◽  
Pressure Sensors ◽  
Cardiac Monitoring ◽  
Cardiac Apex ◽  
Angular Velocities

Changes in the pattern or amplitude of cardiac rotation have been associated with important cardiovascular diseases, including Heart Failure (HF) which is one of the major health problems worldwide. Recent advances in echocardiographic techniques have allowed for non-invasive quantification of cardiac rotation; however, these examinations do not address the continuous monitoring of patient status. We have presented a newly developed implantable, transvenous lead with a tri-axis (3D) MEMS gyroscope incorporated near its tip to measure cardiac apex rotation in the three-dimensional space. We have named it CardioMon for its intended use for cardiac monitoring. If compared with currently proposed implantable systems for HF monitoring based on the use of pressure sensors that can have reliability issues, an implantable motion sensor like a gyroscope holds the premise for more reliable long term monitoring. The first prototypal assembly of the CardioMon lead has been tested to assess the reliability of the 3D gyroscope readings. In vitro results showed that the novel sensorized CardioMon lead was accurate and reliable in detecting angular velocities within the range of cardiac twisting velocities. Animal experiments will be planned to further evaluate the CardioMon lead in in vivo environments and to investigate possible endocardial implantation sites.

scholarly journals Direct Metal Laser Sintering Titanium Dental Implants: A Review of the Current Literature

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
F. Mangano ◽  
L. Chambrone ◽  
R. van Noort ◽  
C. Miller ◽  
P. Hatton ◽  
...  
Keyword(s):  
Dental Implants ◽  
Methodological Quality ◽  
Meta Analysis ◽  
Three Dimensional ◽  
Laser Sintering ◽  
Titanium Implants ◽  
Human Studies ◽  
Direct Metal Laser Sintering ◽  
Titanium Dental Implants

Statement of Problem. Direct metal laser sintering (DMLS) is a technology that allows fabrication of complex-shaped objects from powder-based materials, according to a three-dimensional (3D) computer model. With DMLS, it is possible to fabricate titanium dental implants with an inherently porous surface, a key property required of implantation devices.Objective. The aim of this review was to evaluate the evidence for the reliability of DMLS titanium dental implants and their clinical and histologic/histomorphometric outcomes, as well as their mechanical properties.Materials and Methods. Electronic database searches were performed. Inclusion criteria were clinical and radiographic studies, histologic/histomorphometric studies in humans and animals, mechanical evaluations, andin vitrocell culture studies on DMLS titanium implants. Meta-analysis could be performed only for randomized controlled trials (RCTs); to evaluate the methodological quality of observational human studies, the Newcastle-Ottawa scale (NOS) was used.Results. Twenty-seven studies were included in this review. No RCTs were found, and meta-analysis could not be performed. The outcomes of observational human studies were assessed using the NOS: these studies showed medium methodological quality.Conclusions. Several studies have demonstrated the potential for the use of DMLS titanium implants. However, further studies that demonstrate the benefits of DMLS implants over conventional implants are needed.

scholarly journals BBS7–SHH Signaling Activity Regulates Primary Cilia for Periodontal Homeostasis

2021 ◽  
Vol 9 ◽  
Author(s):  
Pi En Chang ◽  
Shujin Li ◽  
Hyun-Yi Kim ◽  
Dong-Joon Lee ◽  
Yoon Jeong Choi ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Periodontal Ligament ◽  
Primary Cilia ◽  
Periodontal Ligament Cells ◽  
Mechanical Stimuli ◽  
Human Teeth ◽  
Tubule Formation ◽  
Shh Signaling ◽  
Signaling Activity

Objectives: Mechanical stimuli are essential for the maintenance of periodontal ligament (PDL) homeostasis. Although there are several studies on atrophic changes in PDL due to occlusal hypofunction, the underlying mechanism is still unknown. Here, we aimed to explore the changes of gene expression in occlusal hypofunctional PDL and elucidate the related role in maintaining the PDL homeostasis.Methods: To investigate the transcriptomic difference between control and hypofunctional PDL tissue from patients, RNA sequencing was performed on 34 human teeth. The atrophic changes in PDL were evaluated by histological analysis. The effect of the Bardet-Biedl syndrome 7 (BBS7) knockdown was evaluated by the RT-qPCR, Western blot, wound healing, and tubule formation assay.Results: We detected that the expression of BBS7 was downregulated in occlusal hypofunctional PDL through RNA sequencing. Dynamic changes, including the number of periodontal ligament cells, alignment of collagen fibers, diameter of blood vessels, appearance of primary cilia, and torturous oxytalan fibers, were observed following occlusal hypofunction. Furthermore, Sonic hedgehog signaling (Shh) activity was closely associated with BBS7 expression in PDL cells. In addition, the cell migration and angiogenesis were also suppressed by BBS7 knockdown in vitro.Conclusion: We suggest that BBS7 plays an essential role in maintaining Shh signaling activity for PDL homeostasis.

scholarly journals A Scientific Symposium “Artificial Organs Today: From in Vitro Assessment to Human Therapies” on the Occasion of the Celebration of the 40th Anniversary of the Macedonian Academy of Sciences and Arts

PRILOZI ◽  
2020 ◽  
Vol 41 (3) ◽  
pp. 91-93
Author(s):  
Momir H. Polenakovic
Keyword(s):  
Treatment Options ◽  
Artificial Organs ◽  
Artificial Organ ◽  
40Th Anniversary ◽  
System Requirements ◽  
In Vitro Assessment ◽  
Academy Of Sciences ◽  
Organ Replacement ◽  
Ageing Populations

AbstractOn the occasion of the celebration of the 40th anniversary of the Macedonian Academy of Sciences and Arts (MASA) the Macedonian Society of Nephrology, Dialysis, Transplantation and Artificial Organs (MSNDTAO) organized a one day symposium titled: “Artificial Organs Today: From in vitro assessment to human therapies”, on September 28, 2007 at the Macedonian Academy of Sciences and Arts. The following sessions were held: Artificial Organs in Front of the Ageing Populations; System Requirements for Artificial Organ Technology; Tools for Artificial Organ Technology; Extracorporeal Blood Circuits in Organ Replacement Therapies; Treatment Options for Blood Purification Therapies. Presentation were delivered by: Horst Klinkmann, Germany; Jörg Vienken, Germany; Jens Hartmann, Austria; Udo Losert, Austria; Jan Wojcicki, Poland; Helmut Mann and Siegfried Stiller, Germany; Beat Walpoth, Switzerland; Juan F. del Cañizo, Spain; Borce Georgievski, Macedonia; Bernd Stegmayr, Sweden; Momir Polenakovic, Macedonia; Petar Kes, Croatia; Aleksandar Sikole, Macedonia; Wolfgang Ramlow, Germany; Dieter Falkenhagen, Austria. These meetings are significant for the application and development of the artificial organs in patients.

Investigations for Wax Coated 3D Printed Hybrid Patterns for Partial Dentures

2019 ◽  
Author(s):  
Kamaljit Singh Boparai ◽  
Gurpartap Singh ◽  
Rupinder Singh ◽  
Sarabjit Singh
Keyword(s):  
Fused Deposition Modeling ◽  
Three Dimensional ◽  
Surface Hardness ◽  
Dimensional Accuracy ◽  
Acrylonitrile Butadiene Styrene ◽  
Processing Technique ◽  
Patient Specific ◽  
Fused Deposition ◽  
3D Printed

Abstract In this work, 3D printed master patterns of acrylonitrile butadiene styrene (ABS) thermoplastic material have been used for the preparation of Ni-Cr based functional prototypes as partial dentures (PD). The study started with patient specific three dimensional (3D), CAD data (fetched through scanning). This data was used for preparation of .STL file for printing of master patterns on fused deposition modeling (FDM) setup. The 3D printed master patterns were further wax coated to reduce the surface irregularities (as cost effective post processing technique). The hybrid patterns were subjected to investment casting for the preparation of Ni-Cr based PD. The finally prepared functional prototypes as PD were optimized for dimensional accuracy, surface finish and surface hardness as responses. The results are visualized and supported by photomicrographs and in-vitro analysis.

Living the heart in three dimensions: applications of 3D printing in CHD

2019 ◽  
Vol 29 (06) ◽  
pp. 733-743 ◽  
Author(s):  
Mari Nieves Velasco Forte ◽  
Tarique Hussain ◽  
Arno Roest ◽  
Gorka Gomez ◽  
Monique Jongbloed ◽  
...  
Keyword(s):  
3D Printing ◽  
Heart Surgery ◽  
Wide Spectrum ◽  
Three Dimensional ◽  
Structural Heart Disease ◽  
Medical Personnel ◽  
Three Dimensions ◽  
Patient Specific ◽  
3D Printed

AbstractAdvances in biomedical engineering have led to three-dimensional (3D)-printed models being used for a broad range of different applications. Teaching medical personnel, communicating with patients and relatives, planning complex heart surgery, or designing new techniques for repair of CHD via cardiac catheterisation are now options available using patient-specific 3D-printed models. The management of CHD can be challenging owing to the wide spectrum of morphological conditions and the differences between patients. Direct visualisation and manipulation of the patients’ individual anatomy has opened new horizons in personalised treatment, providing the possibility of performing the whole procedure in vitro beforehand, thus anticipating complications and possible outcomes. In this review, we discuss the workflow to implement 3D printing in clinical practice, the imaging modalities used for anatomical segmentation, the applications of this emerging technique in patients with structural heart disease, and its limitations and future directions.

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