scholarly journals Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. albicans

Fermentation ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 28 ◽  
Author(s):  
Anne-Céline Kohler ◽  
Leonardo Venturelli ◽  
Abhilash Kannan ◽  
Dominique Sanglard ◽  
Giovanni Dietler ◽  
...  
Keyword(s):  
Antifungal Drugs ◽  
Mucosal Surface ◽  
Public Health Issue ◽  
Adhesive Properties ◽  
Simple Manner ◽  
Major Public Health Issue ◽  
Force Microscopy ◽  
Fungal Adhesion ◽  
Atomic Force ◽  
Induced Changes

Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the yeast adhesion onto the mucosal surface could lead to potentially highly efficient treatments. In this work, we explored the effect of fibronectin on the nanomotion pattern of different Candida albicans strains by atomic force microscopy (AFM)-based nanomotion detection and correlated the cellular oscillations to the yeast adhesion onto epithelial cells. Preliminary results demonstrate that strongly adhering strains reduce their nanomotion activity upon fibronectin exposure whereas low adhering Candida remain unaffected. These results open novel avenues to explore cellular reactions upon exposure to stimulating agents and possibly to monitor in a rapid and simple manner adhesive properties of C. albicans.

scholarly journals Atomic force microscopy of a ctpA mutant in Rhizobium leguminosarum reveals surface defects linking CtpA function to biofilm formation

Microbiology ◽  
2011 ◽  
Vol 157 (11) ◽  
pp. 3049-3058 ◽  
Author(s):  
Jun Dong ◽  
Karla S. L. Signo ◽  
Elizabeth M. Vanderlinde ◽  
Christopher K. Yost ◽  
Tanya E. S. Dahms
Keyword(s):  
Atomic Force Microscopy ◽  
Mutant Strain ◽  
Biofilm Formation ◽  
Rhizobium Leguminosarum ◽  
Surface Defects ◽  
Ion Concentration ◽  
Adhesive Properties ◽  
Surface Ultrastructure ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy was used to investigate the surface ultrastructure, adhesive properties and biofilm formation of Rhizobium leguminosarum and a ctpA mutant strain. The surface ultrastructure of wild-type R. leguminosarum consists of tightly packed surface subunits, whereas the ctpA mutant has much larger subunits with loose lateral packing. The ctpA mutant strain is not capable of developing fully mature biofilms, consistent with its altered surface ultrastructure, greater roughness and stronger adhesion to hydrophilic surfaces. For both strains, surface roughness and adhesive forces increased as a function of calcium ion concentration, and for each, biofilms were thicker at higher calcium concentrations.

Effect of ampicillin on adhesive properties of bacteria examined by atomic force microscopy

Micron ◽  
2018 ◽  
Vol 112 ◽  
pp. 84-90 ◽  
Author(s):  
Dariusz Laskowski ◽  
Janusz Strzelecki ◽  
Konrad Pawlak ◽  
Hanna Dahm ◽  
Aleksander Balter
Keyword(s):  
Atomic Force Microscopy ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Atomic Force Microscopy of Cell Growth and Division in Staphylococcus aureus

2004 ◽  
Vol 186 (11) ◽  
pp. 3286-3295 ◽  
Author(s):  
Ahmed Touhami ◽  
Manfred H. Jericho ◽  
Terry J. Beveridge
Keyword(s):  
Staphylococcus Aureus ◽  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Cross Wall ◽  
Adhesive Properties ◽  
Reactive Material ◽  
Force Microscopy ◽  
Additional Information ◽  
Atomic Force ◽  
Sequential Images

ABSTRACT The growth and division of Staphylococcus aureus was monitored by atomic force microscopy (AFM) and thin-section transmission electron microscopy (TEM). A good correlation of the structural events of division was found using the two microscopies, and AFM was able to provide new additional information. AFM was performed under water, ensuring that all structures were in the hydrated condition. Sequential images on the same structure revealed progressive changes to surfaces, suggesting the cells were growing while images were being taken. Using AFM small depressions were seen around the septal annulus at the onset of division that could be attributed to so-called murosomes (Giesbrecht et al., Arch. Microbiol. 141:315-324, 1985). The new cell wall formed from the cross wall (i.e., completed septum) after cell separation and possessed concentric surface rings and a central depression; these structures could be correlated to a midline of reactive material in the developing septum that was seen by TEM. The older wall, that which was not derived from a newly formed cross wall, was partitioned into two different surface zones, smooth and gel-like zones, with different adhesive properties that could be attributed to cell wall turnover. The new and old wall topographies are equated to possible peptidoglycan arrangements, but no conclusion can be made regarding the planar or scaffolding models.

Adhesive properties of Staphylococcus epidermidis probed by atomic force microscopy

2011 ◽  
Vol 13 (21) ◽  
pp. 9995 ◽  
Author(s):  
Yifan Hu ◽  
Jens Ulstrup ◽  
Jingdong Zhang ◽  
Søren Molin ◽  
Vincent Dupres
Keyword(s):  
Atomic Force Microscopy ◽  
Staphylococcus Epidermidis ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force

Observation of angiotensin II-induced changes in fixed and live mesangial cells by atomic force microscopy

Micron ◽  
2010 ◽  
Vol 41 (3) ◽  
pp. 220-226 ◽  
Author(s):  
Gi-Ja Lee ◽  
Eun-Jin Park ◽  
Samjin Choi ◽  
Jeong-Hoon Park ◽  
Kyung-Hwan Jeong ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Angiotensin Ii ◽  
Mesangial Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Induced Changes

scholarly journals Adhesion and Mechanical Properties of PDMS-Based Materials Probed with AFM: A Review

2018 ◽  
Vol 56 (1) ◽  
pp. 62-78 ◽  
Author(s):  
S. Vlassov ◽  
S. Oras ◽  
M. Antsov ◽  
I. Sosnin ◽  
B. Polyakov ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Flexible Electronics ◽  
Food Industry ◽  
2D Materials ◽  
Organic Polymer ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force ◽  
Dry Adhesives ◽  
Silicon Based

Abstract Polydimethylsiloxane (PDMS) is the most widely used silicon-based organic polymer, and is particularly known for its unusual rheological properties. PDMS has found extensive usage in various fields ranging from microfluidics and flexible electronics to cosmetics and food industry. In certain applications, like e.g. dry adhesives or dry transfer of 2D materials, adhesive properties of PDMS play crucial role. In this review we focus on probing the mechanical and adhesive properties of PDMS by means of atomic force microscopy (AFM). Main advantages and limitations of AFM-based measurements in comparison to macroscopic tests are discussed.

scholarly journals Assessment of Elasticity and Topography of Aspergillus nidulans Spores via Atomic Force Microscopy

2005 ◽  
Vol 71 (2) ◽  
pp. 955-960 ◽  
Author(s):  
Liming Zhao ◽  
David Schaefer ◽  
Mark R. Marten
Keyword(s):  
Atomic Force Microscopy ◽  
Aspergillus Nidulans ◽  
Fungal Spores ◽  
Protein Structures ◽  
Spore Surface ◽  
Wild Type ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force ◽  
Tapping Mode Afm

ABSTRACT Previous studies have described both surface morphology and adhesive properties of fungal spores, but little information is currently available on their mechanical properties. In this study, atomic force microscopy (AFM) was used to investigate both surface topography and micromechanical properties of Aspergillus nidulans spores. To assess the influence of proteins covering the spore surface, wild-type spores were compared with spores from isogenic rodA + and rodA − strains. Tapping-mode AFM images of wild-type and rodA + spores in air showed characteristic “rodlet” protein structures covering a granular spore surface. In comparison, rodA − spores were rodlet free but showed a granular surface structure similar to that of the wild-type and rodA + spores. Rodlets were removed from rodA + spores by sonication, uncovering the underlying granular layer. Both rodlet-covered and rodlet-free spores were subjected to nanoindentation measurements, conducted in air, which showed the stiffnesses to be 110 ± 10, 120 ± 10, and 300 ± 20 N/m and the elastic moduli to be 6.6 ± 0.4, 7.0 ± 0.7, and 22 ± 2 GPa for wild-type, rodA + and rodA − spores, respectively. These results imply the rodlet layer is significantly softer than the underlying portion of the cell wall.

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