scholarly journals Long-Term Waterlogging as Factor Contributing to Hypoxia Stress Tolerance Enhancement in Cucumber: Comparative Transcriptome Analysis of Waterlogging Sensitive and Tolerant Accessions

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 189
Author(s):  
Kinga Kęska ◽  
Michał Wojciech Szcześniak ◽  
Izabela Makałowska ◽  
Małgorzata Czernicka
Keyword(s):  
Stress Tolerance ◽  
De Novo ◽  
Average Length ◽  
Marker Genes ◽  
Long Term Memory ◽  
Low Oxygen ◽  
Waterlogging Tolerance ◽  
Pre Treatment ◽  
Waterlogging Treatment

Waterlogging (WL), excess water in the soil, is a phenomenon often occurring during plant cultivation causing low oxygen levels (hypoxia) in the soil. The aim of this study was to identify candidate genes involved in long-term waterlogging tolerance in cucumber using RNA sequencing. Here, we also determined how waterlogging pre-treatment (priming) influenced long-term memory in WL tolerant (WL-T) and WL sensitive (WL-S) i.e., DH2 and DH4 accessions, respectively. This work uncovered various differentially expressed genes (DEGs) activated in the long-term recovery in both accessions. De novo assembly generated 36,712 transcripts with an average length of 2236 bp. The results revealed that long-term waterlogging had divergent impacts on gene expression in WL-T DH2 and WL-S DH4 cucumber accessions: after 7 days of waterlogging, more DEGs in comparison to control conditions were identified in WL-S DH4 (8927) than in WL-T DH2 (5957). Additionally, 11,619 and 5007 DEGs were identified after a second waterlogging treatment in the WL-S and WL-T accessions, respectively. We identified genes associated with WL in cucumber that were especially related to enhanced glycolysis, adventitious roots development, and amino acid metabolism. qRT-PCR assay for hypoxia marker genes i.e., alcohol dehydrogenase (adh), 1-aminocyclopropane-1-carboxylate oxidase (aco) and long chain acyl-CoA synthetase 6 (lacs6) confirmed differences in response to waterlogging stress between sensitive and tolerant cucumbers and effectiveness of priming to enhance stress tolerance.

scholarly journals Waterlogging-Stress-Responsive LncRNAs, Their Regulatory Relationships with MiRNAs and Target Genes in Cucumber (Cucumis sativus L.)

2021 ◽  
Vol 22 (15) ◽  
pp. 8197
Author(s):  
Kinga Kęska ◽  
Michał Wojciech Szcześniak ◽  
Adela Adamus ◽  
Małgorzata Czernicka
Keyword(s):  
Target Genes ◽  
Recovery Period ◽  
Sufficient Information ◽  
Low Oxygen ◽  
Waterlogging Tolerance ◽  
Cucumis Sativus L ◽  
Waterlogging Stress ◽  
Non Coding Rna

Low oxygen level is a phenomenon often occurring during the cucumber cultivation period. Genes involved in adaptations to stress can be regulated by non-coding RNA. The aim was the identification of long non-coding RNAs (lncRNAs) involved in the response to long-term waterlogging stress in two cucumber haploid lines, i.e., DH2 (waterlogging tolerant—WL-T) and DH4 (waterlogging sensitive—WL-S). Plants, at the juvenile stage, were waterlogged for 7 days (non-primed, 1xH), and after a 14-day recovery period, plants were stressed again for another 7 days (primed, 2xH). Roots were collected for high-throughput RNA sequencing. Implementation of the bioinformatic pipeline made it possible to determine specific lncRNAs for non-primed and primed plants of both accessions, highlighting differential responses to hypoxia stress. In total, 3738 lncRNA molecules were identified. The highest number (1476) of unique lncRNAs was determined for non-primed WL-S plants. Seventy-one lncRNAs were depicted as potentially being involved in acquiring tolerance to hypoxia in cucumber. Understanding the mechanism of gene regulation under long-term waterlogging by lncRNAs and their interactions with miRNAs provides sufficient information in terms of adaptation to the oxygen deprivation in cucumber. To the best of our knowledge, this is the first report concerning the role of lncRNAs in the regulation of long-term waterlogging tolerance by priming application in cucumber.

Chain processes in cognitive dynamics

10.12737/3399 ◽  
2014 ◽  
Vol 3 (1) ◽  
pp. 70-78
Author(s):  
Комиссаров ◽  
G. Komissarov ◽  
Рубцова ◽  
N. Rubtsova
Keyword(s):  
Present Model ◽  
Average Length ◽  
Random Access ◽  
Long Term Memory ◽  
Cognitive Dynamics ◽  
Chain Explosion ◽  
Favorable Conditions ◽  
Good Agreement ◽  
Reaction Chain

The article discusses the construction of circuits only within random-access memory, additionally limited by attention. We propose a model explaining the features of the dynamics of solving problems on insight using N.N. Semenov’s theory of chain branching chemical reactions. When referring to long-term memory (and return from it with the result on the construction of a representation) the real average length of generalized mental chains can only increase, since it is known that the concepts and objects in it branched and associated with others. In circuit theory, the condition when values of average length chains go to infinity means nonstationary mode of reaction (chain explosion). Thus, in the present model this mode of sharp increase in the average length of the chains is the most favorable conditions for the building the representation – any circuit (with a length of no longer than d) we take to build from existing units operated with all its branches - it is taken in our attention and has no time to break off. These conditions are most favorable for the building and restructuring the representations, and there is the insight moment in our model. Well-known literature data on the experimental investigation of insight and creativity are in good agreement with the requirements of this model towards insight parameters.

Temporal phases of long-term potentiation (LTP): myth or fact?

2015 ◽  
Vol 26 (5) ◽  
pp. 507-546 ◽  
Author(s):  
Abdul-Karim Abbas ◽  
Agnès Villers ◽  
Laurence Ris
Keyword(s):  
Protein Synthesis ◽  
De Novo ◽  
Hippocampal Slices ◽  
Long Term Potentiation ◽  
Long Term Memory ◽  
Protein Synthesis Inhibitor ◽  
Protein Synthesis Inhibitors ◽  
Early Protein ◽  
Term Potentiation

AbstractLong-term potentiation (LTP) remains the most widely accepted model for learning and memory. In accordance with this belief, the temporal differentiation of LTP into early and late phases is accepted as reflecting the differentiation of short-term and long-term memory. Moreover, during the past 30 years, protein synthesis inhibitors have been used to separate the early, protein synthesis-independent (E-LTP) phase and the late, protein synthesis-dependent (L-LTP) phase. However, the role of these proteins has not been formally identified. Additionally, several reports failed to show an effect of protein synthesis inhibitors on LTP. In this review, a detailed analysis of extensive behavioral and electrophysiological data reveals that the presumed correspondence of LTP temporal phases to memory phases is neither experimentally nor theoretically consistent. Moreover, an overview of the time courses of E-LTP in hippocampal slices reveals a wide variability ranging from <1 h to more than 5 h. The existence of all these conflictual findings should lead to a new vision of LTP. We believe that the E-LTP vs. L-LTP distinction, established with protein synthesis inhibitor studies, reflects a false dichotomy. We suggest that the duration of LTP and its dependency on protein synthesis are related to the availability of a set of proteins at synapses and not to the de novo synthesis of plasticity-related proteins. This availability is determined by protein turnover kinetics, which is regulated by previous and ongoing electrical activities and by energy store availability.

scholarly journals FXR1P Limits Long-Term Memory, Long-Lasting Synaptic Potentiation, and De Novo GluA2 Translation

Cell Reports ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. 1402-1416 ◽  
Author(s):  
Denise Cook ◽  
Erin Nuro ◽  
Emma V. Jones ◽  
Haider F. Altimimi ◽  
W. Todd Farmer ◽  
...  
Keyword(s):  
De Novo ◽  
Long Term Memory ◽  
Synaptic Potentiation ◽  
Term Memory

Differential requirement of de novo Arc protein synthesis in the insular cortex and the amygdala for safe and aversive taste long-term memory formation

2018 ◽  
Vol 342 ◽  
pp. 89-93 ◽  
Author(s):  
Kioko Guzmán-Ramos ◽  
Archana Venkataraman ◽  
Jean-Pascal Morin ◽  
Daniel Osorio-Gómez ◽  
Federico Bermúdez-Rattoni
Keyword(s):  
Protein Synthesis ◽  
De Novo ◽  
Insular Cortex ◽  
Memory Formation ◽  
Long Term Memory ◽  
Term Memory ◽  
Aversive Taste

scholarly journals Environmental light is required for maintenance of long-term memory in Drosophila

2019 ◽  
Author(s):  
Show Inami ◽  
Shoma Sato ◽  
Shu Kondo ◽  
Hiromu Tanimoto ◽  
Toshihiro Kitamoto ◽  
...  
Keyword(s):  
De Novo ◽  
Critical Role ◽  
Maintenance Phase ◽  
Camp Response Element Binding ◽  
Long Term Memory ◽  
Term Memory ◽  
Environmental Light ◽  
Element Binding Protein ◽  
The Brain

AbstractLong-term memory (LTM) is stored as functional modifications of relevant neural circuits in the brain. A large body of evidence indicates that the initial establishment of such modifications through the process known as memory consolidation requires learning-dependent transcriptional activation and de novo protein synthesis. However, it remains poorly understood how the consolidated memory is maintained for a long period in the brain, despite constant turnover of molecular substrates. Using the Drosophila courtship-conditioning assay of adult males as a memory paradigm, here, we show that in Drosophila, environmental light plays a critical role in LTM maintenance. LTM is impaired when flies are kept in constant darkness (DD) during the memory maintenance phase. Because light activates the brain neurons expressing the neuropeptide Pigment-dispersing factor (Pdf), we examined the possible involvement of Pdf neurons in LTM maintenance. Temporal activation of Pdf neurons compensated for the DD-dependent LTM impairment, whereas temporal knockdown of Pdf during the memory maintenance phase impaired LTM in light–dark cycles. Furthermore, we demonstrated that the transcription factor cAMP response element-binding protein (CREB) is required in the memory center, mushroom bodies (MBs), for LTM maintenance, and Pdf signaling regulates light-dependent transcription via CREB. Our results demonstrate for the first time that universally available environmental light plays a critical role in LTM maintenance by activating the evolutionarily conserved memory modulator CREB in MBs via the Pdf signaling pathway.Significant StatementTemporary memory can be consolidated into long-term memory (LTM) through de novo protein synthesis and functional modifications of neuronal circuits in the brain. Once established, LTM requires continual maintenance so that it is kept for an extended period against molecular turnover and cellular reorganization that may disrupt memory traces. How is LTM maintained mechanistically? Despite its critical importance, the molecular and cellular underpinnings of LTM maintenance remain elusive. This study using Drosophila is significant because it revealed for the first time in any organism that universally available environmental light plays an essential role in LTM maintenance. Interestingly, light does so by activating the evolutionarily conserved transcription factor cAMP response element-binding protein via peptidergic signaling.

Use of the trimmed Shah permavent tube in the management of glue ear

1994 ◽  
Vol 108 (4) ◽  
pp. 303-306 ◽  
Author(s):  
V. A. Abdullah ◽  
M. B. Pringle ◽  
N. S. Shah
Keyword(s):  
De Novo ◽  
Average Length ◽  
High Grade ◽  
Ventilation Tube ◽  
Middle Ear Effusion ◽  
Extrusion Rate ◽  
Average Length Of Stay ◽  
Glue Ear ◽  
Ear Effusion

AbstractTwenty-five children (mean age six years) with de novo bilateral ear effusions received a ‘trimmed’ high grade silicone (HGS) Shah permavent ventilation tube in one ear and a conventional polyethylene Shah grommet in the other.The extrusion rate and the degree of tympanosclerosis formation was examined. At 29 months the conventional grommet had extruded in 90 per cent of children and a recurrent middle ear effusion was found in over 50 per cent of these ears.The average length of stay for the conventional grommet was 12.5 months. Five permavent tubes had extruded, one was extruding but the remainder were all in place and patent. Comparing ears on each side the amount of tympanosclerosis was worse in the ear with the conventional grommet in 47 per cent of children and worse on the permanent side in 11 per cent of the children.The ‘trimmed permavent’ appears to act as a medium to long-term grommet which self extrudes without serious complications. Its use at the primary operation in young children may save repeated insertions of conventional grommets.

scholarly journals Chemogenetic evidence that rapid neuronal de novo protein synthesis is required for consolidation of long-term memory

2019 ◽  
Author(s):  
Prerana Shrestha ◽  
Pinar Ayata ◽  
Pedro Herrero-Vidal ◽  
Francesco Longo ◽  
Alexandra Gastone ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Translational Control ◽  
De Novo ◽  
Kinase Domain ◽  
Stimulus Generalization ◽  
Long Term Memory ◽  
Term Memory ◽  
The Cost

AbstractTranslational control of memory processes is a tightly regulated process where the coordinated interaction and modulation of translation factors provides a permissive environment for protein synthesis during memory formation. Existing methods used to block translation lack the spatiotemporal precision to investigate cell-specific contributions to consolidation of long-term memories. Here, we have developed a novel chemogenetic mouse resource for cell type-specific and drug-inducible protein synthesis inhibition (ciPSI) that utilizes an engineered version of the catalytic kinase domain of dsRNA-activated protein (PKR). ciPSI allows rapid and reversible phosphorylation of eIF2α causing a block on general translation by 50% in vivo. Using this resource, we discovered that temporally structured pan-neuronal protein synthesis is required for consolidation of long-term auditory threat memory. Targeted protein synthesis inhibition in CamK2α expressing glutamatergic neurons in lateral amygdala (LA) impaired long-term memory, which was recovered with artificial chemogenetic reactivation at the cost of stimulus generalization. Conversely, genetically reducing phosphorylation of eIF2α in CamK2α positive neurons in LA enhanced memory strength, but was accompanied with reduced memory fidelity and behavior inflexibility. Our findings provide evidence for a finely tuned translation program during consolidation of long-term threat memories.

scholarly journals A role for CIM6P/IGF2 receptor in memory consolidation and enhancement

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Xiao-Wen Yu ◽  
Kiran Pandey ◽  
Aaron C Katzman ◽  
Cristina M Alberini
Keyword(s):  
Memory Consolidation ◽  
De Novo ◽  
Critical Role ◽  
Long Term Memory ◽  
Dependent Manner ◽  
Memory Retention ◽  
Memory Enhancement ◽  
Mrna Induction ◽  
Mannose 6 Phosphate

Cation-independent mannose-6-phosphate receptor, also called insulin-like growth factor two receptor (CIM6P/IGF2R), plays important roles in growth and development, but is also extensively expressed in the mature nervous system, particularly in the hippocampus, where its functions are largely unknown. One of its major ligands, IGF2, is critical for long-term memory formation and strengthening. Using CIM6P/IGF2R inhibition in rats and neuron-specific knockdown in mice, here we show that hippocampal CIM6P/IGF2R is necessary for hippocampus-dependent memory consolidation, but dispensable for learning, memory retrieval, and reconsolidation. CIM6P/IGF2R controls the training-induced upregulation of de novo protein synthesis, including increase of Arc, Egr1, and c-Fos proteins, without affecting their mRNA induction. Hippocampal or systemic administration of mannose-6-phosphate, like IGF2, significantly enhances memory retention and persistence in a CIM6P/IGF2R-dependent manner. Thus, hippocampal CIM6P/IGF2R plays a critical role in memory consolidation by controlling the rate of training-regulated protein metabolism and is also a target mechanism for memory enhancement.

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