scholarly journals Identification of Genomic Regions Associated with Concentrations of Milk Fat, Protein, Urea and Efficiency of Crude Protein Utilization in Grazing Dairy Cows

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 456
Author(s):  
Hewa Bahithige Pavithra Chathurangi Ariyarathne ◽  
Martin Correa-Luna ◽  
Hugh Thomas Blair ◽  
Dorian John Garrick ◽  
Nicolas Lopez-Villalobos
Keyword(s):  
Gene Ontology ◽  
New Zealand ◽  
Dairy Cows ◽  
Candidate Genes ◽  
Milk Fat ◽  
Crude Protein ◽  
Single Locus ◽  
Protein Utilization ◽  
Grazing Dairy Cows ◽  
Genomic Regions

The objective of this study was to identify genomic regions associated with milk fat percentage (FP), crude protein percentage (CPP), urea concentration (MU) and efficiency of crude protein utilization (ECPU: ratio between crude protein yield in milk and dietary crude protein intake) using grazing, mixed-breed, dairy cows in New Zealand. Phenotypes from 634 Holstein Friesian, Jersey or crossbred cows were obtained from two herds at Massey University. A subset of 490 of these cows was genotyped using Bovine Illumina 50K SNP-chips. Two genome-wise association approaches were used, a single-locus model fitted to data from 490 cows and a single-step Bayes C model fitted to data from all 634 cows. The single-locus analysis was performed with the Efficient Mixed-Model Association eXpedited model as implemented in the SVS package. Single nucleotide polymorphisms (SNPs) with genome-wide association p-values ≤ 1.11 × 10−6 were considered as putative quantitative trait loci (QTL). The Bayes C analysis was performed with the JWAS package and 1-Mb genomic windows containing SNPs that explained > 0.37% of the genetic variance were considered as putative QTL. Candidate genes within 100 kb from the identified SNPs in single-locus GWAS or the 1-Mb windows were identified using gene ontology, as implemented in the Ensembl Genome Browser. The genes detected in association with FP (MGST1, DGAT1, CEBPD, SLC52A2, GPAT4, and ACOX3) and CPP (DGAT1, CSN1S1, GOSR2, HERC6, and IGF1R) were identified as candidates. Gene ontology revealed six novel candidate genes (GMDS, E2F7, SIAH1, SLC24A4, LGMN, and ASS1) significantly associated with MU whose functions were in protein catabolism, urea cycle, ion transportation and N excretion. One novel candidate gene was identified in association with ECPU (MAP3K1) that is involved in post-transcriptional modification of proteins. The findings should be validated using a larger population of New Zealand grazing dairy cows.

scholarly journals Supplemental concentrates with four levels of crude protein for grazing Dairy Cows

1969 ◽  
Vol 74 (3) ◽  
pp. 281-292
Author(s):  
Paul F. Randel
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Crude Protein ◽  
Energy Requirements ◽  
Net Energy ◽  
Daily Production ◽  
Adequate Quality ◽  
Four Levels ◽  
Grazing Dairy Cows

Four treatments (T1 through T4), based on pelleted concentrates varying in as fed crude protein (CP) contents (10.8, 12.2, 13.4, and 15.3%, respectively), but theoretically isocaloric (1.67-1.70 Mcal net energy/kg), were compared for efficacy as pasture supplements. Eighteen multiparous cows began the experiment individually not later than 8 weeks postpartum. Five were assigned to T1 and T3 and four to T2 and T4. Daily concentrate allowances were according to milk production, but 4.5 kg was set as the arbitrary minimum. Nocturnal rotational grazing was in 12 0.5-ha paddocks of little fertilized, heterogeneous, gramineous swards, which fluctuated in quality from adequate to marginal during 11 1/2 months of experimentation. Cows assigned to the four treatments in order produced 21.3 ± 3.0, 22.5 ± 4.4, 20.3 ± 2.5, and 20.4 ± 4.5 kg of milk daily during the 5 days before commencing the experiment, and 14.4 ± 1.2, 16.8 ± 3.9, 15.2 ± 2.4 and 14.2 ± 2.4 kg during the 32 weeks of experimentation. Although treatments did not differ significantly (P>.05) over-all, T1 dropped in daily production by 2.7 kg during the initial 4-week interval and persistency decreased in high-producing cows; T2 also caused a large early decline in production, but superior persistency thereafter. Mean milk fat percentages were 2.78 ± .49, 2.68 ± .44, 2.88 ± .33, and 3.17 ± .37 for 7, through T4. Early depression of milk fat was probably due to high concentrate and inadequate fiber intakes. Concentrate intake ranged from 5.82 kg in T4 to 7.1 0 kg in T2 over 32 weeks; milk/concentrate ratio ranged from 2.33 in T3 to 2.45 in T4. Only in T1 was appreciable liveweight (20 kg) temporarily lost early in the experiment. Concentrates containing 13 to 14% CP can be tentatively recommended as supplements to pastures of adequate quality, when fed to meet energy requirements of cows producing up to at least 20 kg of milk daily.

Supplementation of grazing dairy cows with a brewers grains/treated straw mixture

1990 ◽  
Vol 1990 ◽  
pp. 46-46 ◽  
Author(s):  
J.D. Leaver ◽  
R.C. Campling
Keyword(s):  
Dairy Cows ◽  
Crude Protein ◽  
Dry Matter ◽  
Barley Straw ◽  
Severe Drought ◽  
Grass Silage ◽  
Metabolisable Energy ◽  
Grazing Dairy Cows ◽  
Similar Cost ◽  
Friesian Cows

Supplementary feeding of grazing dairy cows is often uneconomic, and whilst supplementation with silage (buffer feeding) can be worthwhile, this often leads to a depletion of winter forage stores. In this study, a mixture of brewers grains and treated straw was used as a supplement. Offered as a 1:1 mixture in the dry matter (DM), it is a purchased substitute for grass silage, having a similar cost, and similar metabolisable energy (ME) and crude protein (CP) contents. The high seasonality adjustments to milk price in mid-late season make supplementation potentially worthwhile.Experiments were carried out from April to September in 1988 and 1989, which had moderate and very low rainfall respectively. Each year 20 British Friesian cows which calved December to March (1988 experiment) and February-April (1989) were allocated at random to either treatment B or C. In B, the cows were offered a 1:1 mixture (DM basis) of brewers grains and NaOH treated chopped barley straw for 60 minutes after morning milking. In C, the cows received no supplement. Both groups were fed 1.0 kg/day of concentrates in the milking parlour. Due to the severe drought in 1989, concentrate feeding was increased to 5.0 kg/day for all cows during the last 4 weeks of the experiment. Also, urea-treated whole crop wheat was fed at a level of 2.5 kg DM/day during the last 7 days.

scholarly journals Betaine Improves Milk Yield in Grazing Dairy Cows Supplemented with Concentrates at High Temperatures

Animals ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 57 ◽  
Author(s):  
Frank Dunshea ◽  
Kehinde Oluboyede ◽  
Kristy DiGiacomo ◽  
Brian Leury ◽  
Jeremy Cottrell
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
The Mean ◽  
Fat Yield ◽  
Grazing Dairy Cows ◽  
Daily Milk Yield ◽  
Betaine Supplementation ◽  
Daily Milk

Betaine is an organic osmolyte sourced from sugar beet that accumulates in plant cells undergoing osmotic stress. Since the accumulation of betaine lowers the energy requirements of animals and, therefore, metabolic heat production, the aim of this experiment was to investigate if betaine supplementation improved milk yield in grazing dairy cows in summer. One hundred and eighteen Friesian × Holstein cows were paired on days in milk and, within each pair, randomly allocated to a containing treatment of either 0 or 2 g/kg natural betaine in their concentrate ration for approximately 3 weeks during February/March 2015 (summer in Australia). The mean maximum February temperature was 30 °C. Cows were allocated approximately 14 kg dry matter pasture and 7.5 kg of concentrate pellets (fed in the milking shed) per cow per day and were milked through an automatic milking system three times per day. Betaine supplementation increased average daily milk yield by over 6% (22.0 vs. 23.4 kg/day, p < 0.001) with the response increasing as the study progressed as indicated by the interaction (p < 0.001) between betaine and day. Milk fat % (p = 0.87), milk protein % (p = 0.90), and milk somatic cell count (p = 0.81) were unchanged by dietary betaine. However, betaine supplementation increased milk protein yield (677 vs. 719 g/day, p < 0.001) and fat yield (874 vs. 922 g/day, p < 0.001) with responses again being more pronounced as the study progressed. In conclusion, dietary betaine supplementation increased milk and component yield during summer in grazing dairy cows.

Effect of timing of protein supplementation on the performance of lactating dairy cows

2005 ◽  
Vol 45 (4) ◽  
pp. 337
Author(s):  
B. C. Granzin
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Protein Supplementation ◽  
Net Energy ◽  
Maize Silage ◽  
Holstein Friesian ◽  
Grazing Dairy Cows ◽  
And Control ◽  
Friesian Cows

Two experiments were undertaken to determine the effect of timing of protein supplementation on performance of grazing, lactating Holstein–Friesian cows fed maize silage and grain-based concentrate. In experiment 1, 36 cows were fed 0.8 kg DM/day of solvent-extracted cottonseed meal (CSM) either as 1 meal at 1200 hours with maize silage (CSM 1200) or at 1530 hours with concentrate (CSM 1530), or in 2 meals at 0600 and 1530 hours with concentrate (CSM 600 + 1530). In experiment 2, 36 cows were either fed no CSM (control) or fed 1.0 kg DM/day as either CSM 1200 or CSM 600 + 1530. In experiment 1, daily yields of 4% fat-corrected milk (FCM) and milk fat for CSM 600 + 1530 were significantly higher than for CSM 1530 with respective means of 22.8 v. 20.7 L and 895 v. 804 g. Daily yields of FCM and milk fat for CSM 1200 were intermediate (21.7 L and 841 g/cow, respectively). A similar trend for daily protein yield per cow was noted (712, 695 and 666 g for CSM 600 + 1530, 1200 and 1530, respectively). In experiment 2, milk yield differed numerically between CSM 600 + 1530 and other treatments, with means (± s.e.d.) of 24.7 ± 0.78, 22.9 ± 0.78 and 22.9 ± 0.78 L/cow.day for CSM 600 + 1530, CSM 1200 and control, respectively. Mean (± s.e.d.) net energy requirements for milk production and liveweight change tended to be lower for the control (68 ± 3.6 MJ/cow.day) as opposed to CSM 600 + 1530 (79 ± 3.6 MJ/cow.day) and CSM 1200 (76 ± 3.6 MJ/cow.day). Cumulative time where rumen degradable nitrogen:rumen degradable dry matter was less than 22 g/kg were 2, 2 and 3 h for CSM 600 + 1530, CSM 1200 and CSM 1530, respectively, in experiment 1, and 6, 4 and 2 h for the control, 1200 and CSM 600 + 1530, respectively, in experiment 2. No differences in rumen ammonia-N concentrations were noted between treatments in experiment 1. In experiment 2, a significantly lower mean (± s.e.d.) rumen ammonia-N concentration was recorded for the control at 1530 hours (62 ± 14.1 mg/dL) in comparison to CSM 600 + 1530 (114 ± 14.1 mg/dL) and CSM 1200 (119 ± 14.1 mg/dL). These experiments show that for grazing dairy cows supplemented with maize silage and grain-based concentrate, feeding a daily aliquot of CSM as 2 meals at 0600 and 1530 hours rather than 1 meal at 1200 or 1530 hours improves milk production.

Effects of pasture type and grazing management in autumn on the performance of dairy cows in late lactation and on subsequent pasture productivity

1984 ◽  
Vol 24 (126) ◽  
pp. 312 ◽  
Author(s):  
KR King ◽  
CR Stockdale
Keyword(s):  
Dairy Cows ◽  
White Clover ◽  
Milk Fat ◽  
Dry Matter ◽  
Grazing Management ◽  
Daily Growth ◽  
Feeding Experiments ◽  
Herbage Quality ◽  
Grazing Dairy Cows

An experiment at Kyabram, Victoria, studied the effects of pasture allowance on the production of grazing dairy cows in late lactation (autumn). Cows strip-grazed paspalum dominant or ryegrass-white clover perennial pastures for 60 d from March to May. Mean pasture allowances were 23.2, 13.3 or 6.9 kg digestible dry matter/cow.d. Reduced pasture allowance during late lactation reduced milk yield and body condition. This occurred because pasture intake was positively related to pasture allowance. Each additional kilogram of dry matter (DM) eaten produced 0.93 kg milk and 0.045 kg milk fat. Cows grazing ryegrass-white . clover pastures produced more milk and milk products at equivalent DM intakes than did cows grazing paspalum dominant pastures, and they were in better condition. This was due to herbage quality: cows grazing ryegrass-white clover pastures were able to select higher quality diets than could those grazing paspalum dominant pastures. When the results were compared with those from two previous stall-feeding experiments, the marginal efficiencies for milk production in all experiments were not significantly different; an extra 1.19 kg fat corrected milk was produced for each additional kilogram of digestible dry matter of pasture eaten. . The pastures were not grazed from May to September but their growth and quality were measured during this period. The amount of pasture left at drying off influenced the growth and quality of the pastures throughout winter: daily growth declined by 3.31 kg DM/ha between May and July, and by 6 56 kg DM/ha between July and September, as herbage yield at drying off (May) increased by each additional tonne of DM per hectare. Also, the ryegrass-white clover pastures grew more herbage at equivalent DM yields than did the paspalum dominant pastures. The implications of these results for grazing management are discussed.

scholarly journals The effect of fat supplementation of concentrates on digestion and utilization of energy by productive dairy cows.

1981 ◽  
Vol 29 (2) ◽  
pp. 79-92 ◽  
Author(s):  
Y. van der Honing ◽  
B.J. Wieman ◽  
A. Steg ◽  
B. van Donselaar
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Crude Protein ◽  
Latin Square ◽  
Soya Bean ◽  
Metabolizable Energy ◽  
Simultaneous Reduction ◽  
Gross Energy ◽  
The One ◽  
Lipid Supplement

Two energy balance experiments each used 6 high-yielding dairy cows. In the first, a 3 X 3 Latin square with one replicate, rations were of 7 kg hay and 11 to 14 kg of concentrates having crude protein 251 g and gross energy 17.9 MJ/kg, given alone or with 5% tallow or 5% soya bean oil. Three weeks of adaptation were followed by 10 to 12 days when excreta were collected and gas exchange estimated. Digestibility and metabolizable energy of concentrates and hay were also assessed using wethers fed to maintenance only. Intakes of DM and digestibility of nutrients other than lipid did not differ among treatments with either sheep or cows. There were no apparent differences in rumen fermentation in the one cannulated cow used, and energy loss in urine was also unaffected. Lipid supplement reduced methane loss. Milk yield and milk energy were increased with tallow, with a simultaneous reduction of milk fat and percentage of protein. Soya bean oil reduced the percentage of milk fat and milk energy produced. The second trial, using similar animals and with similar management had a change-over design in which the concentrate was given alone or with 7% tallow. Results confirmed those of trial 1. The effect of level of feeding on the efficiency of utilization of energy is discussed. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Effect of dietary supplementation with fish oil during the transition period on milk production, plasma metabolites and postpartum anoestrus interval in grazing dairy cows

2011 ◽  
Vol 51 (5) ◽  
pp. 481 ◽  
Author(s):  
A. Mendoza ◽  
D. Crespi ◽  
A. Hernández ◽  
N. Roura ◽  
H. Valentín ◽  
...  
Keyword(s):  
Fish Oil ◽  
Dairy Cows ◽  
Milk Yield ◽  
Postpartum Period ◽  
Milk Fat ◽  
Dietary Supplementation ◽  
Plasma Metabolites ◽  
Ovarian Activity ◽  
Dominant Follicle ◽  
Grazing Dairy Cows

Inclusion of fish oil (FO) in the diet of dairy cows could improve the energy status and milk yield and reduce the interval from calving to first postpartum ovulation, but the effects may differ depending on the specific period of supplementation. To test these hypotheses, 28 pregnant Holstein heifers were randomly assigned to four treatments in a 2 × 2 factorial design: prepartum oral drench of FO (0 or 140 g/cow.day) for 21 days before calving, and/or postpartum oral drench of FO (0 or 190 g/cow.day) for 21 days after calving. Prepartum, heifers grazed in native pastures with supplements of wheat silage and wheat bran, while postpartum, cows grazed on improved pastures with addition of concentrates and corn silage. No interaction between prepartum and postpartum FO was detected for any variable analysed. Prepartum and postpartum total DM intake, milk yield, protein yield and percentage were not different among treatments. Milk fat percentage (3.83 versus 3.34 ± 0.10%) and yield (0.88 versus 0.73 ± 0.04 kg/day) decreased with postpartum FO but were not affected by prepartum FO. Prepartum and postpartum body condition score and plasma concentration of non-esterified fatty acids and cholesterol were not different among treatments, but urea concentration was higher in cows drenched with FO during prepartum (5.91 versus 4.64 ± 0.35 mM). Proportion of cows that ovulated the dominant follicle of the first postpartum wave and days to first postpartum ovulation, as determined by ovarian ultrasonography, were not affected by drenching with FO, but size of the dominant follicle in the first wave was decreased when FO was drenched only during the pre- or postpartum period compared with drenching in both periods. Dietary supplementation with FO during the postpartum period affected milk fat synthesis but more research is needed to establish its effects on resumption of ovarian activity in grazing dairy cows.

Effect of cereal grain and fibre supplements on the fatty acid composition of milk fat of grazing dairy cows in early lactation

2003 ◽  
Vol 70 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Chakra Wijesundera ◽  
Zhiping Shen ◽  
William J Wales ◽  
Dawn E Dalley
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Acid Composition ◽  
Milk Fat ◽  
Physiological State ◽  
Early Lactation ◽  
Cereal Grain ◽  
Grazing Dairy Cows

Two experiments were undertaken to determine the effects of cereal grain and fibre (hay or straw) supplements on the fatty acid composition of milk fat of grazing dairy cows in early lactation. In both experiments, grain supplements significantly increased (P<0·05) the proportion of the endogenously synthesized 10[ratio ]0–16[ratio ]0 fatty acids. Of the C18 acids, the proportion of 18[ratio ]0 and 18[ratio ]3 was significantly decreased (P<0·05) by grain supplementation, while that of 18[ratio ]2 was significantly increased (P<0·05). Irrespective of diet, 18[ratio ]1 trans-11 was the most dominant trans 18[ratio ]1 isomer in milk fat. In the first experiment, the proportions of the 18[ratio ]1 trans-11 isomer and conjugated linoleic acid (CLA, 18[ratio ]2 cis-9, trans-11) were highest for the pasture-only diets, and significantly (P<0·05) decreased with grain supplementation. The opposite result was observed in the second experiment, conducted in a different dairy region, suggesting that factors such as the quality of pasture on offer and the physiological state of the cow could affect the content of CLA and trans fatty acids in milk fat. In both experiments, there was a significant positive linear relationship between CLA and 18[ratio ]1 trans-11. Fibre supplements had little effect on the fatty acid composition of the milk.

scholarly journals PROTEIN LEVELS IN GRASS SILAGE-BASED TOTAL MIXED RATIONS FOR DAIRY COWS IN MIDLACTATION

1984 ◽  
Vol 64 (2) ◽  
pp. 435-442 ◽  
Author(s):  
P. L. BURGESS ◽  
J. W. G. NICHOLSON
Keyword(s):  
Dairy Cows ◽  
Protein Level ◽  
Milk Fat ◽  
Crude Protein ◽  
Dry Matter ◽  
Ammonia Nitrogen ◽  
Plasma Urea ◽  
Protein Levels ◽  
Grass Silage ◽  
Nitrogen Intake

Total mixed rations containing three levels of crude protein (10, 13 and 16%) were fed ad libitum to a total of 48 cows in midlactation in two experiments. Rations were composed of 60% wilted timothy silage and 40% concentrates on a dry matter basis. Supplemental protein was supplied as soybean meal. Daily nitrogen intake per cow averaged 263, 377 and 510 g in exp. 1, and 305, 435 and 509 g in exp. 2 for the three rations, respectively. Cows fed rations containing 10% crude protein consumed less (P < 0.05) total dry matter and lost more (P < 0.01) body weight than cows fed rations with either 13 or 16% crude protein. Milk production was not affected by protein level in exp. 1 but in exp. 2, 10% crude protein decreased (P < 0.01) actual milk and 4% fat corrected milk yields. Milk fat and protein percentages tended to increase with increasing dietary protein level. Rumen acetate tended to decline while propionate increased (P < 0.05) at higher crude protein levels. Rumen ammonia nitrogen and plasma urea nitrogen levels were closely related to nitrogen intake. Key words: Dairy cows, grass silage, protein, solubility

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