scholarly journals Methylphosphonate Degradation and Salt-Tolerance Genes of Two Novel Halophilic Marivita Metagenome-Assembled Genomes from Unrestored Solar Salterns

Genes ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 148
Author(s):  
Clifton P. Bueno de Mesquita ◽  
Jinglie Zhou ◽  
Susanna Theroux ◽  
Susannah G. Tringe
Keyword(s):  
High Salinity ◽  
Phylogenetic Analyses ◽  
Draft Genome ◽  
Compatible Solute ◽  
Aerobic Bacteria ◽  
Genome Sequences ◽  
Restored Wetlands ◽  
Genes Encoding ◽  
Salinity Levels ◽  
Reference Genomes

Aerobic bacteria that degrade methylphosphonates and produce methane as a byproduct have emerged as key players in marine carbon and phosphorus cycles. Here, we present two new draft genome sequences of the genus Marivita that were assembled from metagenomes from hypersaline former industrial salterns and compare them to five other Marivita reference genomes. Phylogenetic analyses suggest that both of these metagenome-assembled genomes (MAGs) represent new species in the genus. Average nucleotide identities to the closest taxon were <85%. The MAGs were assembled with SPAdes, binned with MetaBAT, and curated with scaffold extension and reassembly. Both genomes contained the phnCDEGHIJLMP suite of genes encoding the full C-P lyase pathway of methylphosphonate degradation and were significantly more abundant in two former industrial salterns than in nearby reference and restored wetlands, which have lower salinity levels and lower methane emissions than the salterns. These organisms contain a variety of compatible solute biosynthesis and transporter genes to cope with high salinity levels but harbor only slightly acidic proteomes (mean isoelectric point of 6.48).

scholarly journals Draft Genome Sequences of Two Enterobacter cloacae subsp. cloacae Strains Isolated from Australian Hematology Patients with Bacteremia

2017 ◽  
Vol 5 (33) ◽  
Author(s):  
Lex E. X. Leong ◽  
David Shaw ◽  
Lito Papanicolas ◽  
Diana Lagana ◽  
Ivan Bastian ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Draft Genome ◽  
Enterobacter Cloacae ◽  
Opportunistic Pathogen ◽  
Healthy Individuals ◽  
Genome Sequences ◽  
Content Type ◽  
Extended Spectrum ◽  
Genes Encoding

ABSTRACT Enterobacter cloacae is a common member of the gut microbiota in healthy individuals. However, it is also an opportunistic pathogen, capable of causing bacteremia. We report the draft genomes of two Enterobacter cloacae subspecies cloacae strains isolated from hematology patients with bacteremia. Both isolates carry genes encoding extended-spectrum β-lactamases.

scholarly journals 'Candidatus Phytoplasma sacchari’, a novel taxon - associated with Sugarcane Grassy Shoot (SCGS) disease

2020 ◽  
Author(s):  
Kiran Kirdat ◽  
Bhavesh Tiwarekar ◽  
Vipool Thorat ◽  
Shivaji Sathe ◽  
Yogesh Shouche ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Indian Subcontinent ◽  
Phylogenetic Analyses ◽  
Draft Genome ◽  
Housekeeping Genes ◽  
Rrna Gene ◽  
Genome Sequences ◽  
Phytoplasma Strain ◽  
White Leaf

Sugarcane Grassy Shoot (SCGS) disease is known to be related to Rice Yellow Dwarf (RYD) phytoplasmas (16SrXI-B group) which are found predominantly in sugarcane growing areas of the Indian subcontinent and South-East Asia. The 16S rRNA gene sequences of SCGS phytoplasma strains belonging to the 16SrXI-B group share 98.07 % similarity with ‘Ca. Phytoplasma cynodontis’ strain BGWL-C1 followed by 97.65 % similarity with ‘Ca. P. oryzae’ strain RYD-J. Being placed distinctly away from both the phylogenetically related species, the taxonomic identity of SCGS phytoplasma is unclear and confusing. We attempted to resolve the phylogenetic positions of SCGS phytoplasma based on the phylogenetic analysis of 16S rRNA gene (>1500 bp), nine housekeeping genes (>3500 aa), core genome phylogeny (>10 000 aa) and OGRI values. The draft genome sequences of SCGS phytoplasma (strain SCGS) and Bermuda Grass White leaf (BGWL) phytoplasma (strain LW01), closely related to ‘Ca. P. cynodontis’, were obtained. The SCGS genome was comprised of 29 scaffolds corresponding to 505 173 bp while LW01 assembly contained 21 scaffolds corresponding to 483 935 bp with the fold coverages over 330× and completeness over 90 % for both the genomes. The G+C content of SCGS was 19.86 % while that of LW01 was 20.46 %. The orthoANI values for the strain SCGS against strains LW01 was 79.42 %, and dDDH values were 22. Overall analysis reveals that SCGS phytoplasma forms a distant clade in RYD group of phytoplasmas. Based on phylogenetic analyses and OGRI values obtained from the genome sequences, a novel taxon ‘Candidatus Phytoplasma sacchari’ is proposed.

scholarly journals Draft genome sequences of strains CBS6241 and CBS6242 of the basidiomycetous yeast Filobasidium floriforme

2021 ◽  
Author(s):  
Marco Alexandre Guerreiro ◽  
Steven Ahrendt ◽  
Jasmyn Pangilinan ◽  
Cindy Chen ◽  
Mi Yan ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Plant Cell Wall ◽  
Draft Genome ◽  
Genome Sequences ◽  
Protein Coding ◽  
Biotechnological Potential ◽  
Pheromone Receptor ◽  
Genes Encoding ◽  
Cryptococcus Species ◽  
Mat Locus

Abstract The Tremellomycetes are a species-rich group within the basidiomycete fungi; however, most analyses of this group to date have focused on pathogenic Cryptococcus species within the order Tremellales. Recent genome-assisted studies of other Tremellomycetes have identified interesting features with respect to biotechnological applications as well as the evolution of genes involved in mating and sexual development. Here, we report genome sequences of two strains of Filobasidium floriforme, a species from the order Filobasidiales, which branches basally to the Tremellales, Trichosporonales and Holtermanniales. The assembled genomes of strains CBS6241 and CBS6242 are 27.4 Mb and 26.4 Mb in size, respectively, with 8314 and 7695 predicted protein-coding genes. Overall sequence identity at nucleic acid level between the strains is 97%. Among the predicted genes are pheromone precursor and pheromone receptor genes as well as two genes encoding homedomain (HD) transcription factors, which are predicted to be part of the mating type (MAT) locus. Sequence analysis indicates that CBS6241 and CBS6242 carry different alleles for both the pheromone/receptor genes as well as the HD transcription factors. Orthology inference identified 1482 orthogroups exclusively found in F. floriforme, some of which were involved in carbohydrate transport and metabolism. Subsequent CAZyme repertoire characterization identified 267 and 247 enzymes for CBS6241 and CBS6242, respectively, the second highest number of CAZymes among the analyzed Tremellomycete species. Additionally, F. floriforme contains five CAZymes absent in other species and several plant-cell-wall degrading CAZymes with the highest copy number in Tremellomycota, indicating the biotechnological potential of this species.

scholarly journals Draft genome sequences of two oriental melons, Cucumis melo L. var. makuwa

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Ah-Young Shin ◽  
Namjin Koo ◽  
Seungill Kim ◽  
Young Mi Sim ◽  
Doil Choi ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
Draft Genome ◽  
Biological Properties ◽  
Genome Sequences ◽  
Functional Studies ◽  
Oriental Melon ◽  
Horticultural Traits ◽  
Northeast Asian ◽  
Cucumis Melo L ◽  
Reference Genomes

Abstract Oriental melon (Cucumis melo L. var. makuwa) is one of the most important cultivated cucurbits, and is grown widely in Northeast Asian countries. With increasing interest in its biological properties and economic importance, oriental melon has become an attractive model crop for studying various horticultural traits. A previous genome sequence of the melon was constructed from a homozygous double-haploid line. Thus, individual reference genomes are required to perform functional studies and further breeding applications. Here, we report draft genome sequences of two oriental melons, Chang Bougi and SW3. The assembled 344 Mb genome of Chang Bougi was obtained with scaffold N50 1.0 Mb, and 36,235 genes were annotated. The 354 Mb genome of SW3 was assembled with scaffold N50 1.6 Mb, and has 38,173 genes. These newly constructed genomes will enable studies of fruit development, disease resistance, and breeding applications in the oriental melon.

scholarly journals The kleboxymycin biosynthetic gene cluster is encoded by several species belonging to the Klebsiella oxytoca complex

2020 ◽  
Author(s):  
Preetha Shibu ◽  
Frazer McCuaig ◽  
Anne L. McCartney ◽  
Magdalena Kujawska ◽  
Lindsay J. Hall ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Virulence Factors ◽  
Phylogenetic Analyses ◽  
Klebsiella Oxytoca ◽  
Draft Genome ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Accession Number ◽  
Genome Sequences ◽  
Or Gene

ABSTRACTAs part of ongoing studies with clinically relevant Klebsiella spp., we characterized the genomes of three clinical GES-5-positive strains originally identified as Klebsiella oxytoca. Average nucleotide identity and phylogenetic analyses showed the strains to be Klebsiella michiganensis. In addition to encoding GES-5, the strains encoded SHV-66, a β-lactamase not previously identified in K. michiganensis. The strains further encoded a range of virulence factors and the kleboxymycin biosynthetic gene cluster (BGC), previously only found in K. oxytoca strains and one strain of Klebsiella grimontii. This BGC, associated with antibiotic-associated haemorrhagic colitis, has not previously been reported in K. michiganensis, and this finding led us to carry out a wide-ranging study to determine the prevalence of this BGC in Klebsiella spp. Of 7,170 publicly available Klebsiella genome sequences screened, 88 encoded the kleboxymycin BGC. All BGC-positive strains belonged to the K. oxytoca complex, with strains of four (K. oxytoca, K. pasteurii, K. grimontii, K. michiganensis) of the six species of the complex found to encode the complete BGC. In addition to being found in K. grimontii strains isolated from preterm infants, the BGC was found in K. oxytoca and K. michiganensis metagenome-assembled genomes recovered from neonates. Detection of the kleboxymycin BGC across the K. oxytoca complex may be of clinical relevance and this cluster should be included in databases characterizing virulence factors, in addition to those characterizing BGCs.Data statementSupplementary data associated with this article are available from figshare.Data summaryDraft genome sequences for PS_Koxy1, PS_Koxy2 and PS_Koxy4 have been deposited with links to BioProject accession number PRJNA562720 in the NCBI BioProject database.IMPACT STATEMENTExtended analyses of the genomes of Klebsiella spp. have revealed the kleboxymycin biosynthetic gene cluster (BGC) is restricted to species of the Klebsiella oxytoca complex (K. oxytoca, K. michiganensis, K. pasteurii and K. grimontii). Species- and/or gene-specific differences in the cluster’s sequences may be relevant to virulence of K. oxytoca and related species. The finding of the kleboxymycin BGC in the preterm infant gut microbiota may have implications for disease presentation in a subset of neonates.

scholarly journals Comprehensive Draft Genome Analyses of Three Rockfishes (Scorpaeniformes, Sebastiscus) via Genome Survey Sequencing

2021 ◽  
Vol 43 (3) ◽  
pp. 2048-2058
Author(s):  
Chenghao Jia ◽  
Tianyan Yang ◽  
Takashi Yanagimoto ◽  
Tianxiang Gao
Keyword(s):  
Phylogenetic Analyses ◽  
Complete Mitochondrial Genome ◽  
Economic Value ◽  
Draft Genome ◽  
Whole Genome ◽  
Genome Sequences ◽  
Genome Data ◽  
Genome Survey ◽  
Genome Wide ◽  
Genome Analyses

Sebastiscus species, marine rockfishes, are of essential economic value. However, the genomic data of this genus is lacking and incomplete. Here, whole genome sequencing of all species of Sebastiscus was conducted to provide fundamental genomic information. The genome sizes were estimated to be 802.49 Mb (S. albofasciatus), 786.79 Mb (S. tertius), and 776.00 Mb (S. marmoratus) by using k-mer analyses. The draft genome sequences were initially assembled, and genome-wide microsatellite motifs were identified. The heterozygosity, repeat ratios, and numbers of microsatellite motifs all suggested possibly that S. tertius is more closely related to S. albofasciatus than S. marmoratus at the genetic level. Moreover, the complete mitochondrial genome sequences were assembled from the whole genome data and the phylogenetic analyses genetically supported the validation of Sebastiscus species. This study provides an important genome resource for further studies of Sebastiscus species.

scholarly journals Echinicola Salinicaeni sp. nov., A Novel Bacterium Isolated from Saltern

2021 ◽  
Author(s):  
Han Zhao ◽  
Chun-Yi Song ◽  
Rui Yin ◽  
Yan-Jun Yi ◽  
Shuai-Ting Yun ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Draft Genome ◽  
Carotenoid Biosynthesis ◽  
Amino Acid Identity ◽  
Glycoside Hydrolases ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Resistance Pathway ◽  
Genes Encoding

Abstract A Gram-stain-negative, aerobiotic, pink, motile, rod-shaped bacterium, designated P51 T was isolated from saline silt samples in Yantai, China. The novel isolate was able to grow at 4–42 °C (optimum 33 °C), pH 4.0–9.0 (optimum 7.0) and with 0–11.0% NaCl (optimum 4.0%, w/v). It could grow at 4 °C, which was different from the neighbour strains. The draft genome consisted of 4111 genes with a total length of 5139782 bp and 39.9% G + C content. The 16S rRNA gene sequence analysis indicated that strain P51 T was a member of the genus Echinicola and most closely related to ‘ Echinicola shivajiensis ’ with pair-wise sequence similarity of 97.7%. Genome analysis identified genes encoding proteins relating to carbon source utilization such as glycoside hydrolases and glycosyl transferases; carotenoid biosynthesis pathway and β -lactam resistance pathway were observed. Strain P51 T shared average nucleotide identity value below 84.7%, average amino acid identity value between of 70.8 – 89.3%, digital DNA-DNA hybridization identity of between 17.9–28.2% with the closely related type strains within the genus Echinicola . The sole menaquinone was MK-7; the major fatty acids were iso-C 15:0 , summed feature 3(C 16:1 ω 7 c and/or C 16:1 ω 6 c ), summed feature 4 (anteiso-C 17:1 B and/or iso-C 17:1 I) and summed feature 9 (iso-C 17:1 ω 9 c and/or 10-methyl C 16:0 ); the polar lipids included one phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, three unidentified aminolipids and one unknown lipid. Based on phenotypic, chemotaxonomic, and phylogenetic analyses, strain P51 T represents a novel species of the genus Echinicola , for which the name Echinicola salinicaeni sp. nov. is proposed. The type strain is P51 T (KCTC 82513 T = MCCC 1K04413 T ).

scholarly journals Draft Genome Sequences of Klebsiella pneumoniae Strains Isolated from Immunocompromised NOD-scid Gamma Research Mice

2019 ◽  
Vol 8 (42) ◽  
Author(s):  
Anthony Mannion ◽  
Niora Fabian ◽  
Melissa Stair ◽  
Jody Dzink-Fox ◽  
Sebastian E. Carrasco ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Draft Genome ◽  
Illumina Miseq ◽  
Genome Sequences ◽  
Pathogenic Potential ◽  
Content Type ◽  
Nsg Mice ◽  
Genes Encoding ◽  
Clinical Illness ◽  
Biomedical Research Institute

Thirteen Klebsiella pneumoniae isolates cultured from feces, intestines, liver, lungs, and blood from immunocompromised NOD-scid gamma (NSG) mice with clinical illness, housed at a biomedical research institute, were sequenced using Illumina MiSeq technology for elucidation of pathogenic potential and genes encoding antibiotic resistance.

scholarly journals Draft Genome Sequences of Two Strains of a Newly Described Species, Sphingobacterium cellulitidis

2017 ◽  
Vol 5 (38) ◽  
Author(s):  
Torsten Seemann ◽  
Dieter M. Bulach ◽  
Glen Carter ◽  
M. John Albert
Keyword(s):  
Type Strain ◽  
Draft Genome ◽  
The Other ◽  
Genome Sequences ◽  
Content Type ◽  
Reference Genomes

ABSTRACT The draft genome sequences of two strains of a newly described species, Sphingobacterium cellulitidis, have been determined. The type strain originated from cellulitis of a toe of a patient and the other strain from the environment. The sequences will provide the reference genomes of the new Sphingobacterium species.

scholarly journals Draft Genome Sequences of Campylobacter jejuni Strains Isolated from Poultry

2020 ◽  
Vol 9 (7) ◽  
Author(s):  
Basanta R. Wagle ◽  
Daya Marasini ◽  
Indu Upadhyaya ◽  
Sandip Shrestha ◽  
Komala Arsi ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Broiler Chickens ◽  
Draft Genome ◽  
Toxin Production ◽  
Intestinal Colonization ◽  
Wild Type ◽  
Genome Sequences ◽  
Content Type ◽  
Genes Encoding ◽  
Production Stress

Four wild-type Campylobacter jejuni strains isolated from the cecal contents of broiler chickens were sequenced. The average genome size was 1,622,170 bp, with 1,667 to 1,761 coding sequences and 47 to 51 RNAs. Multiple genes encoding motility, intestinal colonization, toxin production, stress tolerance, and multidrug resistance were present in all the strains.

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