scholarly journals RNAi-Mediated Gene Silencing in a Gonad Organ Culture to Study Sex Determination Mechanisms in Sea Turtle

Genes ◽  
2013 ◽  
Vol 4 (2) ◽  
pp. 293-305 ◽  
Author(s):  
Itzel Sifuentes-Romero ◽  
Horacio Merchant-Larios ◽  
Sarah Milton ◽  
Norma Moreno-Mendoza ◽  
Verónica Díaz-Hernández ◽  
...  
Keyword(s):  
Organ Culture ◽  
Gene Silencing ◽  
Sex Determination ◽  
Sea Turtle ◽  
Sex Determination Mechanisms

scholarly journals Exogenous estradiol alters gonadal growth and timing of temperature sex determination in gonads of sea turtle

2015 ◽  
Vol 408 (1) ◽  
pp. 79-89 ◽  
Author(s):  
Verónica Díaz-Hernández ◽  
Alejandro Marmolejo-Valencia ◽  
Horacio Merchant-Larios
Keyword(s):  
Sex Determination ◽  
Sea Turtle ◽  
Gonadal Growth

Environmental Sex Determination Mechanisms in Reptiles

2013 ◽  
Vol 7 (1-3) ◽  
pp. 95-103 ◽  
Author(s):  
H. Merchant-Larios ◽  
V. Díaz-Hernández
Keyword(s):  
Sex Determination ◽  
Environmental Sex Determination ◽  
Sex Determination Mechanisms

scholarly journals Sex Determination During Inflorescence Bud Differentiation in Monoecious Pistacia chinensis Bunge

Forests ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 202 ◽  
Author(s):  
Qian Bai ◽  
Chenyi Zhu ◽  
Xia Lei ◽  
Tao Cao ◽  
Shuchai Su ◽  
...  
Keyword(s):  
Sex Determination ◽  
Early Stage ◽  
Sex Differentiation ◽  
Banding Patterns ◽  
Vegetative Period ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Sex Determination Mechanisms

Pistacia chinensis Bunge is widely acknowledged to be dioecious, but rare monoecious individuals have been found. However, the origin of monoecism and the sex differentiation of different sex types remain intriguing questions. Here, sex expressions were explored by identification of sex-associated DNA markers, determination of the sex stability after grafting, and histological characterization of inflorescence bud development using anatomical analysis. The results showed that (1) although polymorphisms among individuals existed, the banding patterns of Polymerase Chain Reaction (PCR) products for different sex types on the same monoecious tree were consistent; (2) the sex expressions of grafted trees were not consistent with those of scions, indicating that monoecism probably did not originate from a stable bud mutation; and (3) both males and females underwent a bisexual period, then the stamen primordia in female buds degenerated into the second round tepals, while the pistil primordia in male buds gradually disappeared. During the sex differentiation phase, female buds were spindle-shaped, while the male buds were full teardrop-shaped, and male buds were bigger than female buds. Taken together, no sex-associated DNA marker was found, sex expressions were unstable after grafting, and the alternative sex organs appeared in the early stage of sex differentiation, suggesting that sex determination occurred during floral development instead of the early vegetative period. These results indicated that the sex expressions may be affected by environmental factors, increasing the understanding of sex determination mechanisms in P. chinensis and other species.

Expression profiles of Dax1, Dmrt1, and Sox9 during temperature sex determination in gonads of the sea turtle Lepidochelys olivacea

2002 ◽  
Vol 129 (1) ◽  
pp. 20-26 ◽  
Author(s):  
L.C Torres Maldonado ◽  
A Landa Piedra ◽  
N Moreno Mendoza ◽  
A Marmolejo Valencia ◽  
A Meza Martı́nez ◽  
...  
Keyword(s):  
Sex Determination ◽  
Expression Profiles ◽  
Sea Turtle ◽  
Lepidochelys Olivacea

Ancestral state reconstruction analysis of hymenopteran sex determination mechanisms

2009 ◽  
Vol 22 (8) ◽  
pp. 1762-1769 ◽  
Author(s):  
M. K. ASPLEN ◽  
J. B. WHITFIELD ◽  
J. G. DE BOER ◽  
G. E. HEIMPEL
Keyword(s):  
Sex Determination ◽  
Ancestral State Reconstruction ◽  
Ancestral State ◽  
State Reconstruction ◽  
Sex Determination Mechanisms

scholarly journals An Investigation of ZZ/ZW and XX/XY Sex Determination Systems in North African Catfish (Clarias gariepinus, Burchell, 1822)

2021 ◽  
Vol 11 ◽  
Author(s):  
Dung Ho My Nguyen ◽  
Thitipong Panthum ◽  
Jatupong Ponjarat ◽  
Nararat Laopichienpong ◽  
Ekaphan Kraichak ◽  
...  
Keyword(s):  
Sex Determination ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Nucleotide Polymorphisms ◽  
North African ◽  
The North ◽  
Determination System ◽  
Commercial Breeding ◽  
Sex Determination System ◽  
Sex Determination Mechanisms

An investigation of sex-specific loci may provide important insights into fish sex determination strategies. This may be useful for biotechnological purposes, for example, to produce all-male or all-female fish for commercial breeding. The North African catfish species, Clarias gariepinus, has been widely adopted for aquaculture because its superior growth and disease resistance render the species suitable for hybridization with other catfish to improve the productivity and quality of fish meat. This species has either a ZZ/ZW or XX/XY sex determination system. Here, we investigate and characterize these systems using high-throughput genome complexity reduction sequencing as Diversity Arrays Technology. This approach was effective in identifying moderately sex-linked loci with both single-nucleotide polymorphisms (SNPs) and restriction fragment presence/absence (PA) markers in 30 perfectly sexed individuals of C. gariepinus. However, SNPs based markers were not found in this study. In total, 41 loci met the criteria for being moderately male-linked (with male vs. female ratios 80:20 and 70:30), while 25 loci were found to be moderately linked to female sex. No strictly male- or female-linked loci were detected. Seven moderately male-linked loci were partially homologous to some classes of transposable elements and three moderately male-linked loci were partially homologous to functional genes. Our data showed that the male heterogametic XX/XY sex determination system should co-exist with the ZZ/ZW system in C. gariepinus. Our finding of the co-existence of XX/XY and ZZ/ZW systems can be applied to benefit commercial breeding of this species in Thailand. This approach using moderately sex-linked loci provides a solid baseline for revealing sex determination mechanisms and identify potential sex determination regions in catfish, allowing further investigation of genetic improvements in breeding programs.

scholarly journals Algal Sex Determination and the Evolution of Anisogamy

2019 ◽  
Vol 73 (1) ◽  
pp. 267-291 ◽  
Author(s):  
James Umen ◽  
Susana Coelho
Keyword(s):  
Sex Determination ◽  
Sexual Reproduction ◽  
Green Algae ◽  
Life Histories ◽  
Mating Systems ◽  
Chromosome Evolution ◽  
Sex Chromosome ◽  
Photosynthetic Eukaryotes ◽  
Sex Determination Mechanisms ◽  
Shed Light

Algae are photosynthetic eukaryotes whose taxonomic breadth covers a range of life histories, degrees of cellular and developmental complexity, and diverse patterns of sexual reproduction. These patterns include haploid- and diploid-phase sex determination, isogamous mating systems, and dimorphic sexes. Despite the ubiquity of sexual reproduction in algae, their mating-type-determination and sex-determination mechanisms have been investigated in only a limited number of representatives. These include volvocine green algae, where sexual cycles and sex-determining mechanisms have shed light on the transition from mating types to sexes, and brown algae, which are a model for UV sex chromosome evolution in the context of a complex haplodiplontic life cycle. Recent advances in genomics have aided progress in understanding sexual cycles in less-studied taxa including ulvophyte, charophyte, and prasinophyte green algae, as well as in diatoms.

scholarly journals Multi-Tissue Transcriptome Analysis Identifies Key Sexual Development-Related Genes of the Ornate Spiny Lobster (Panulirus ornatus)

Genes ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 1150
Author(s):  
Tomer Ventura ◽  
Jennifer C. Chandler ◽  
Tuan V. Nguyen ◽  
Cameron J. Hyde ◽  
Abigail Elizur ◽  
...  
Keyword(s):  
Sex Determination ◽  
Sexual Development ◽  
Molecular Mechanisms ◽  
Sex Chromosome ◽  
Spiny Lobster ◽  
Homology Search ◽  
Previous Knowledge ◽  
Key Factors ◽  
Factors Associated ◽  
Sex Determination Mechanisms

Sexual development involves the successive and overlapping processes of sex determination, sexual differentiation, and ultimately sexual maturation, enabling animals to reproduce. This provides a mechanism for enriched genetic variation which enables populations to withstand ever-changing environments, selecting for adapted individuals and driving speciation. The molecular mechanisms of sexual development display a bewildering diversity, even in closely related taxa. Many sex determination mechanisms across animals include the key family of “doublesex- and male abnormal3-related transcription factors” (Dmrts). In a few exceptional species, a single Dmrt residing on a sex chromosome acts as the master sex regulator. In this study, we provide compelling evidence for this model of sex determination in the ornate spiny lobster Panulius ornatus, concurrent with recent reports in the eastern spiny lobster Sagmariasus verreauxi. Using a multi-tissue transcriptomic database established for P. ornatus, we screened for the key factors associated with sexual development (by homology search and using previous knowledge of these factors from related species), providing an in-depth understanding of sexual development in decapods. Further research has the potential to close significant gaps in our understanding of reproductive development in this ecologically and commercially significant order.

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