The Role of the E2F Transcription Factor Family in UV-Induced Apoptosis

Hcm1 is a member of the forkhead transcription factor family involved in segregation, spindle pole dynamics, and budding in Saccharomyces cerevisiae. Our group described the role of Hcm1 in mitochondrial biogenesis and stress resistance, and in the cellular adaptation to mitochondrial respiratory metabolism when nutrients decrease. Regulation of Hcm1 activity occurs at the protein level, subcellular localization, and transcriptional activity. Here we report that the amount of protein increased in the G1/S transition phase when the factor accumulated in the nucleus. In the G2/M phases, the Hcm1 amount decreased, and it was translocated outside the nucleus with a network-like localization. Preparation of highly purified mitochondria by a sucrose gradient density demonstrated that Hcm1 colocalized with mitochondrial markers, inducing expression of COX1, a mitochondrial encoded subunit of cytochrome oxidase, in the G2/M phases. Taken together, these results show a new localization of Hcm1 and suggest that it acts as a mitochondrial transcription factor regulating the metabolism of this organelle.

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Role of DBP in the Circadian Oscillatory Mechanism

Molecular and Cellular Biology ◽

10.1128/mcb.20.13.4773-4781.2000 ◽

2000 ◽

Vol 20 (13) ◽

pp. 4773-4781 ◽

Cited By ~ 141

Author(s):

Shun Yamaguchi ◽

Shigeru Mitsui ◽

Lily Yan ◽

Kazuhiro Yagita ◽

Shigeru Miyake ◽

...

Keyword(s):

Transcription Factor ◽

Leucine Zipper ◽

The Other ◽

Suprachiasmatic Nuclei ◽

Transcription Factor Family ◽

Transcript Levels ◽

Oscillatory Mechanism ◽

Central Clock ◽

E Boxes

ABSTRACT Transcript levels of DBP, a member of the PAR leucine zipper transcription factor family, exhibit a robust rhythm in suprachiasmatic nuclei, the mammalian circadian center. Here we report that DBP is able to activate the promoter of a putative clock oscillating gene,mPer1, by directly binding to the mPer1promoter. The mPer1 promoter is cooperatively activated by DBP and CLOCK-BMAL1. On the other hand, dbp transcription is activated by CLOCK-BMAL1 through E-boxes and inhibited by the mPER and mCRY proteins, as is the case for mPer1. Thus, a clock-controlled dbp gene may play an important role in central clock oscillation.

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The R2R3-MYB transcription factor family in Taxus chinensis: identification, characterization, expression profiling and posttranscriptional regulation analysis

PeerJ ◽

10.7717/peerj.8473 ◽

2020 ◽

Vol 8 ◽

pp. e8473

Author(s):

Xinling Hu ◽

Lisha Zhang ◽

Iain Wilson ◽

Fenjuan Shao ◽

Deyou Qiu

Keyword(s):

Transcription Factor ◽

Posttranscriptional Regulation ◽

Gene Families ◽

Myb Transcription Factor ◽

Taxus Chinensis ◽

Transcription Factor Family ◽

Myb Genes ◽

And Function ◽

R2r3 Myb

The MYB transcription factor family is one of the largest gene families playing regulatory roles in plant growth and development. The MYB family has been studied in a variety of plant species but has not been reported in Taxus chinensis. Here we identified 72 putative R2R3-MYB genes in T. chinensis using a comprehensive analysis. Sequence features, conversed domains and motifs were characterized. The phylogenetic analysis showed TcMYBs and AtMYBs were clustered into 36 subgroups, of which 24 subgroups included members from T. chinensis and Arabidopsis thaliana, while 12 subgroups were specific to one species. This suggests the conservation and specificity in structure and function of plant R2R3-MYBs. The expression of TcMYBs in various tissues and different ages of xylem were investigated. Additionally, miRNA-mediated posttranscriptional regulation analysis revealed that TcMYBs were the targets of miR858, miR159 and miR828, suggesting the posttranscriptional regulation of MYBs is highly conserved in plants. The results provide a basis for further study the role of TcMYBs in the regulation of secondary metabolites of T. chinensis.

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Involvement of E2F transcription factor family in cancer

European Journal of Cancer ◽

10.1016/j.ejca.2005.08.005 ◽

2005 ◽

Vol 41 (16) ◽

pp. 2403-2414 ◽

Cited By ~ 99

Author(s):

P.K. Tsantoulis ◽

V.G. Gorgoulis

Keyword(s):

Transcription Factor ◽

Transcription Factor Family ◽

E2f Transcription Factor

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Role of Transcription Factor CaNdt80p in Cell Separation, Hyphal Growth, and Virulence in Candida albicans

Eukaryotic Cell ◽

10.1128/ec.00325-09 ◽

2010 ◽

Vol 9 (4) ◽

pp. 634-644 ◽

Cited By ~ 47

Author(s):

Adnane Sellam ◽

Christopher Askew ◽

Elias Epp ◽

Faiza Tebbji ◽

Alaka Mullick ◽

...

Keyword(s):

Cell Wall ◽

Transcription Factor ◽

Candida Albicans ◽

Cell Separation ◽

Hyphal Growth ◽

Functional Domain ◽

Transcription Factor Family ◽

Content Type ◽

Genes Encoding

ABSTRACT The NDT80/PhoG transcription factor family includes ScNdt80p, a key modulator of the progression of meiotic division in Saccharomyces cerevisiae. In Candida albicans, a member of this family, CaNdt80p, modulates azole sensitivity by controlling the expression of ergosterol biosynthesis genes. We previously demonstrated that CaNdt80p promoter targets, in addition to ERG genes, were significantly enriched in genes related to hyphal growth. Here, we report that CaNdt80p is indeed required for hyphal growth in response to different filament-inducing cues and for the proper expression of genes characterizing the filamentous transcriptional program. These include noteworthy genes encoding cell wall components, such as HWP1, ECE1, RBT4, and ALS3. We also show that CaNdt80p is essential for the completion of cell separation through the direct transcriptional regulation of genes encoding the chitinase Cht3p and the cell wall glucosidase Sun41p. Consistent with their hyphal defect, ndt80 mutants are avirulent in a mouse model of systemic candidiasis. Interestingly, based on functional-domain organization, CaNdt80p seems to be a unique regulator characterizing fungi from the CTG clade within the subphylum Saccharomycotina. Therefore, this study revealed a new role of the novel member of the fungal NDT80 transcription factor family as a regulator of cell separation, hyphal growth, and virulence.

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Atypical E2Fs: new players in the E2F transcription factor family

Trends in Cell Biology ◽

10.1016/j.tcb.2009.01.002 ◽

2009 ◽

Vol 19 (3) ◽

pp. 111-118 ◽

Cited By ~ 116

Author(s):

Tim Lammens ◽

Jing Li ◽

Gustavo Leone ◽

Lieven De Veylder

Keyword(s):

Transcription Factor ◽

Transcription Factor Family ◽

E2f Transcription Factor

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The role of the DNA-binding One Zinc Finger (DOF) transcription factor family in plants

Plant Science ◽

10.1016/j.plantsci.2013.03.016 ◽

2013 ◽

Vol 209 ◽

pp. 32-45 ◽

Cited By ~ 118

Author(s):

Mélanie Noguero ◽

Rana Muhammad Atif ◽

Sergio Ochatt ◽

Richard D. Thompson

Keyword(s):

Transcription Factor ◽

Dna Binding ◽

Zinc Finger ◽

Transcription Factor Family ◽

Dof Transcription Factor

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Impacts of a new transcription factor family

The Journal of Cell Biology ◽

10.1083/jcb.200406097 ◽

2004 ◽

Vol 166 (6) ◽

pp. 765-768 ◽

Cited By ~ 20

Author(s):

Said Hashemolhosseini ◽

Michael Wegner

Keyword(s):

Transcription Factor ◽

Drosophila Melanogaster ◽

Dna Binding ◽

Family Member ◽

Parathyroid Gland ◽

Dna Binding Domain ◽

Transcription Factor Family ◽

Master Regulators ◽

Zinc Cations

GCM proteins constitute a small transcription factor family with a DNA-binding domain exhibiting a novel fold composed of two subdomains rigidly held together by coordination of one of two structural zinc cations. In all known cases, GCM proteins exert the role of master regulators: the prototypical family member determines gliogenesis in Drosophila melanogaster, whereas mammalian GCM proteins orchestrate divergent aspects of development and physiology in placenta, kidney, thymus, and parathyroid gland. Recent data point to an involvement of GCM proteins in different pathological contexts, such as preeclampsia, hyper- or hypoparathyroidism, and parathyroid gland tumors.

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