scholarly journals Integrated Metabolome and Transcriptome Analysis Provide Insights into the Effects of Grafting on Fruit Flavor of Cucumber with Different Rootstocks

2019 ◽  
Vol 20 (14) ◽  
pp. 3592 ◽  
Author(s):  
Li Miao ◽  
Qinghua Di ◽  
Tianshu Sun ◽  
Yansu Li ◽  
Ying Duan ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Expression Patterns ◽  
Sugar Metabolism ◽  
Myb Transcription Factor ◽  
Chemical Production ◽  
Cucumis Sativus L ◽  
Cucumber Fruit ◽  
Valine Biosynthesis ◽  
Fruit Flavor

Rootstocks frequently exert detrimental effects on the fruit quality of grafted cucumber (Cucumis sativus L.) plants. To understand and ultimately correct this deficiency, a transcriptomic and metabolomic comparative analysis was performed among cucumber fruits from non-grafted plants (NG), and fruits from plants grafted onto different rootstocks of No.96 and No.45 (Cucurbita moschata. Duch), known to confer a different aroma and taste. We found remarkable changes in the primary metabolites of sugars, organic acids, amino acids, and alcohols in the fruit of the grafted cucumber plants with different rootstocks, compared to the non-grafted ones, especially No.45. We identified 140, 131, and 244 differentially expressed genes (DEGs) in the comparisons of GNo.96 vs. NG, GNo.45 vs. NG, and GNo.45 vs. GNo.96. The identified DEGs have functions involved in many metabolic processes, such as starch and sucrose metabolism; the biosynthesis of diterpenoid, carotenoid, and zeatin compounds; and plant hormone signal transduction. Members of the HSF, AP2/ERF-ERF, HB-HD-ZIP, and MYB transcription factor families were triggered in the grafted cucumbers, especially in the cucumber grafted on No.96. Based on a correlation analysis of the relationships between the metabolites and genes, we screened 10 candidate genes likely to be involved in sugar metabolism (Fructose-6-phosphate and trehalose), linoleic acid, and amino-acid (isoleucine, proline, and valine) biosynthesis in grafted cucumbers, and then confirmed the gene expression patterns of these genes by qRT-PCR. The levels of TPS15 (Csa3G040850) were remarkably increased in cucumber fruit with No.96 rootstock compared with No.45, suggesting changes in the volatile chemical production. Together, the results of this study improve our understanding of flavor changes in grafted cucumbers, and identify the candidate genes involved in this process.

scholarly journals Comprehensive Characterization of Fruit Volatiles and Nutritional Quality of Three Cucumber (Cucumis sativus L.) Genotypes from Different Geographic Groups after Bagging Treatment

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 294 ◽  
Author(s):  
Nan Shan ◽  
Zengyu Gan ◽  
Jing Nie ◽  
Huan Liu ◽  
Zhenyu Wang ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Nutritional Quality ◽  
Expression Patterns ◽  
Relative Proportion ◽  
Soluble Sugars ◽  
Hydroperoxide Lyase ◽  
Cucumis Sativus L ◽  
Cucumber Fruit ◽  
Fruit Flavor

Bagging is widely practiced to produce high quality and unblemished fruit; however, little is currently known about the effect of bagging on flavor and nutritional quality of cucumber fruits. Here we determined the influence of bagging on fruit quality of cucumber (Cucumis sativus L.) using three genotypes from different geographic groups. Exocarp chlorophyll and carotenoid levels were significantly decreased by bagging, accompanied by color change. Ascorbate content in bagged fruits decreased to some extent, while contents of soluble sugars, starch, and cellulose were comparable with those of control fruits. Compositions related to fruit flavor quality could be enhanced largely through bagging treatment, with elevation of the relative proportion of C6 aldehyde, as well as (E,Z)-2,6-nonadienal/(E)-2-nonenal ratio, and linoleic/α-linolenic acid ratio. Lipoxygenase and hydroperoxide lyase, two key enzymes in the production of volatiles, displayed distinctive transcript expression patterns and trends in changes of enzymatic activity in the bagged fruits of different genotypes. Overall, this study assesses the information on changing characteristics of fruit volatile composition and nutritional quality among different cucumber genotypes after bagging treatment. Results of this study would contribute to providing reference for mechanism study and cultivation conditions to improve cucumber fruit flavor to a considerable degree.

scholarly journals Interactive Effects of Grafting Techniques and Scion-Rootstocks Combinations on Vegetative Growth, Yield and Quality of Cucumber (Cucumis sativus L.)

Agronomy ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 288 ◽  
Author(s):  
Rana Shahzad Noor ◽  
Zhi Wang ◽  
Muhammad Umair ◽  
Muhammad Yaseen ◽  
Muhammad Ameen ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Fruit Quality ◽  
Vegetative Growth ◽  
Interactive Effect ◽  
Growth Yield ◽  
Fruit Yield ◽  
Lagenaria Siceraria ◽  
Cucumis Sativus L ◽  
Cucumber Fruit

The density of herbaceous crops creates a suitable environment to produce pathogens in the soil that intensify the attack of pathogens traditionally controlled by disinfectant, which are mostly prohibited and unlisted because of their toxicity. Grafting is an alternative technique to enhance abiotic stress tolerance and reduce root diseases due to soil-borne pathogens, thus enhancing crop production. This research study was conducted during the crop season of 2017 and 2018 in order to investigate the interactive effect of different grafting techniques of hybrid scion onto local rootstocks on plants survival, plant phenological growth, fruit yield and fruit quality under a controlled environment. The hybrid cucumber was also planted self-rooted. The cucumber (Cucumis sativus L.) cv. Kalaam F1, Syngenta was grafted onto four local cucurbitaceous rootstocks; ridge gourd (Luffa operculate Cogn.), bitter gourd (Momordica charantia L.), pumpkin (Cucurbita pepo L.), bottle gourd (Lagenaria siceraria (Molina) Standl.) using splice grafting, tongue approach, single cotyledon and hole insertion grafting techniques and self-rooted hybrid cucumber under greenhouse conditions. The experimental results indicated that all local cucurbitaceous rootstocks showed a high compatibility with hybrid cucumber scion in the splice grafting method compared to other grafting and non-grafted methods. Lagenaria siceraria rootstocks were found highly compatible with cucumber cv Kalaam scion which gave significantly maximum plant survival rates (95%) due to high sap contents, high SPAD value, better vegetative growth and maximum fruit yield when compared with other rootstocks by employing the splice grafting method followed by tongue approach, single cotyledon and hole insertion grafting while the fruit quality of all rootstocks was observed to be similar. The non-grafted cucumber cv. Kalaam F1 showed significant results of plant vegetative growth, fruit development and fruit quality and encountered grafting methods while the lowest result were associated with the hole insertion grafting method in all scion/rootstock combinations. The grafted plants have no significant effect on cucumber fruit dry matter and fruit quality while the fruit mineral compositions (N, P, K, Ca and Mg) were higher among grafted and non-grafted plant fruits. The results indicate that grafting hybrid cucumber onto four local cucurbitaceous rootstocks influenced growth, yield and fruit quality. Grafting can be alternative and control measure for soil-borne disease and to enhance cucumber production.

scholarly journals Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant

2019 ◽  
Author(s):  
Liyun Wan ◽  
Yong Lei ◽  
Liying Yan ◽  
Yue Liu ◽  
Manish Kumar Pandey ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Seed Coat ◽  
Coat Color ◽  
Expression Patterns ◽  
Tca Cycle ◽  
Tissue Expression ◽  
Processing Efficiency ◽  
Hormone Synthesis ◽  
Mbw Complex

Abstract Background: Coat color determines both appearance and nutrient quality of peanut. White seed coat in peanut can enhance the processing efficiency and quality of peanut oil.An integrative analysis of transcriptomes, metabolomes and histocytology was performed on a white seed coat peanut mutant (wsc) and its wild type to investigate the regulatory mechanisms underlying color pigmentation. Result：Metabolomes revealed flavonoids were redirected in wsc, while multi-omics analyses of wsc mutant seeds and testae uncovered WSC influenced the flavonoids biosynthesis in testa as well as suberin formation, glycolysis, the tricarboxylic acid (TCA) cycle and amino acid metabolism. The mutation also enhanced brassinosteroid (BR), gibberellin (GA), and jasmonic acid (JA) biosynthesis as well as abscisic acid (ABA), auxin (AUX), BR and JA signaling. Further, co-expression analysis showed that flavonol synthase (FLS) genes co-expressed with MYB-bHLH-WD40 (MBW) complex member genes. Combining tissue expression patterns, genetic analyses, and the annotation of common differentially expressed genes (DEGs) for these three stages revealed that three testa specific expressed candidate genes, Araip.M7RY3, Aradu.R8PMF and Araip.MHR6K were likely responsible for the white testa phenotype. WSC might be regulated expression competition between FLS and dihydroflavonol 4-reductase (DRF) by controlling hormone synthesis and signaling as well as the MBW complex. Conclusions: The results of this study therefore provide both candidate genes and novel approaches that can be applied to improve peanut with desirable seed coat color and flavonoid quality.

scholarly journals PENGARUH PENGGUNAAN PUPUK ORGANIK KASCING DAN HORMON GIBERELIN (GA3) TERHADAP PRODUKSI DAN KUALITAS BUAH MENTIMUN (Cucumis sativus L.)

2019 ◽  
Vol 2 (3) ◽  
pp. 102
Author(s):  
Ayna Devita Trisna Putri ◽  
Miswar Miswar
Keyword(s):  
Organic Fertilizer ◽  
Organic Fertilizers ◽  
Cucumis Sativus L ◽  
Potential Productivity ◽  
Group Design ◽  
Cucumber Fruit ◽  
Significant Difference ◽  
Fruit Formation ◽  
Plant Control

ABSTRACT Cucumber is a horticultural product which becomes one type of fruit vegetable and has a great demand in the community. The potential productivity of cucumber itself is still relatively low, that is in 2016 amounting to 10.19 tons / ha, this also occurs in the productivity of local cucumbers which are quite low, but until now there are still many cultivated by farmers in Jember area. In addition to the increase in production, it is also necessary to improve the quality of fruit which affects the competitiveness of the selling value of cucumber fruit. Regarding the increase in production and quality of fruit, the efforts need to be made to improve cultivation methods. One of them is the use of organic fertilizers which contain more complex nutrients and the application of plant regulators. One of the organic fertilizers, vermicompost organic fertilizer, is the result of decomposition by worms which affects plant growth and the application of gibberellins (GA3), it can improve fruit quality and increase the percentage of fruit formation. This study aimed to determine the effect of using the combination of vermicompost organic fertilizer and the addition of giberelin (GA3) on cucumber plants by using a randomized group design (RGD) with 2 factors and 3 replications. The first factor was giving vermicompost organic fertilizer, namely (K0) 0 g/plant (control), (K1) 120 g/plant (3 tons/ha), (K2) 240 g/plant (6 tons/ha), (K3) 360 g/plant (9 tons/ha). The second factor was the addition of gibberellin concentrations (GA3), namely (G0) 0 ppm, (G1) 50 ppm, (G2) 100 ppm and (G3) 150 ppm. The data obtained were then analyzed using variance analysis, if between treatments had a significant difference, then further tests were carried out by using Duncan's Multiple Distance Test at the level of 5%. The best treatment ofvermicompost organic fertilizer dosage and giberelin hormone concentration was found in the treatment of 360 g/plant or 9 tons/ha (K3) at the optimal concentration of 100 ppm giberelin hormone. Key words: Cucumber, Vermicompost fertilizer, Gibberellin (GA3), Production and Quality of Fruit ABSTRAK Mentimun merupakan produk hortikultura yang menjadi salah satu jenis sayuran buah yang banyak diminati oleh masyarakat. Potensi produktivitas mentimun sendiri masih tergolong rendah yaitu pada tahun 2016 sebesar 10,19 ton/ha, hal ini juga terjadi pada produktivitas mentimun jenis lokal yang cukup rendah, namun sampai saat ini masih banyak dibudidayakan oleh petani pada daerah Jember. Selain peningkatan terhadap produksi, perlu juga dilakukan perbaikan kualitas buah yang berpengaruh pada daya saing nilai jual buah mentimun. Terkait peningkatan produksi dan mutu buah, maka perlu dilakukan upaya perbaikan cara budidaya. Salah satunya adalah penggunaan pupuk organik yang mengandung unsur hara yang lebih kompleks dan pengaplikasian zat pengatur tumbuhan. Salah satu pupuk organik yaitu pupuk organik kascing, yang merupakan hasil dekomposisi oleh cacing yang berpengaruh terhadap pertumbuhan tanaman dan aplikasi giberelin (GA3) yang mampu meningkatkan kualitas buah dan meningkatkan persentase pembentukan buah. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi penggunaan pupuk kascing dan pemberian hormon giberelin (GA3) pada tanaman mentimun, menggunakan Rancangan Acak Kelompok (RAK) dengan 2 faktor dan 3 ulangan. Faktor pertama pemberian dosis pupuk kascing yaitu, (K0) 0 g/tanaman (kontrol), (K1) 120 g/tanaman (3 ton/ha), (K2) 240 g/tanaman (6 ton/ha), (K3) 360 g/tanaman (9 ton/ha). Faktor kedua pemberian konsentrasi giberelin (GA3) yaitu (G0) 0 ppm, (G1) 50 ppm, (G2) 100 ppm dan (G3) 150 ppm. Data yang diperoleh selanjutnya dianalisis menggunakan analisis sidik ragam, jika antar perlakuan mengalami perbedaan yang signifikan, maka dilakukan uji lanjut dengan menggunakan Uji Jarak Berganda Duncan pada taraf 5%. Perlakuan dosis pupuk kascing dan konsentrasi hormon Giberelin terbaik terdapat pada perlakuan 360 g/tanaman atau 9 ton/ha (K3) pada konsentrasi optimal hormon giberelin 100 ppm. Kata Kunci: Mentimun, Pupuk Kascing, Giberelin (GA3), Produksi dan Kualitas Buah

scholarly journals Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant

2019 ◽  
Author(s):  
Liyun Wan ◽  
Yong Lei ◽  
Liying Yan ◽  
Yue Liu ◽  
Manish Kumar Pandey ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Seed Coat ◽  
Coat Color ◽  
Expression Patterns ◽  
Tca Cycle ◽  
Tissue Expression ◽  
Processing Efficiency ◽  
Hormone Synthesis ◽  
Mbw Complex

Abstract Background: Coat color determines both appearance and nutrient quality of peanut. White seed coat in peanut can enhance the processing efficiency and quality of peanut oil.An integrative analysis of transcriptomes, metabolomes and histocytology was performed on wsc mutant and its wild type to investigate the regulatory mechanisms underlying color pigmentation. Result：Metabolomes revealed flavonoids were redirected in wsc, while multi-omics analyses of wsc mutant seeds and testae uncovered WSC influenced the flavonoids biosynthesis in testa as well as suberin formation, glycolysis, the TCA cycle and amino acid metabolism. The mutation also enhanced BR, GA, and JA biosynthesis as well as ABA, AUX, BR and JA signaling. Further, co-expression analysis showed that FLS genes co-expressed with MBW complex member genes. Combining tissue expression patterns, genetic analyses, and the annotation of common DEGs for these three stages revealed that three testa specific expressed candidate genes, Araip.M7RY3, Aradu.R8PMF and Araip.MHR6K were likely responsible for the white testa phenotype. WSC might be regulated expression competition between FLS and DRF by controlling hormone synthesis and signaling as well as the MBW complex. Conclusions: The results of this study therefore provide both candidate genes and novel approaches that can be applied to improve peanut with desirable seed coat color and flavonoid quality.

scholarly journals Genome-wide analysis of OVATE family proteins in cucumber (Cucumis sativus L.)

2019 ◽  
Author(s):  
Lijie Han ◽  
Xiaofei Song ◽  
Zhongyi Wang ◽  
Xiaofeng Liu ◽  
Liying Yan ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Expression Patterns ◽  
Ectopic Expression ◽  
Reproductive Organs ◽  
Cucumis Sativus L ◽  
Hormone Response Elements ◽  
Genome Wide ◽  
Cucumber Fruit ◽  
Specific Proteins ◽  
Gibberellin Treatment

Abstract Background: OVATE family proteins (OFPs) are plant-specific proteins with the conserved OVATE domain that regulating plant growth and development. Although these OFPs have been studied in several species, the biological functions of this OFP gene family remain largely unknown in cucumber (Cucumis sativus L.). Results: In this study, we identified 19 CsOFPs in cucumber. This CsOFPs are distributed on seven chromosomes and can be divided into four subgroups. Most CsOFP genes are expressed in reproductive organs although have different expression patterns. Cis-elements analysis showed that there are six kinds of hormone response elements in CsOFPs and exogenous gibberellin treatment leads to a ‘first increase then decrease’ expression pattern of CsOFP7, CsOFP11 and CsOFP12. Ectopic expression of CsOFP11 in Arabidopsis resulted in shorter and blunt siliques. Conclusions: Together, these results indicated that CsOFPs may play important roles in cucumber fruit development.

scholarly journals The Formation of Fruit Quality in Cucumis sativus L.

2021 ◽  
Vol 12 ◽  
Author(s):  
Juping Zhang ◽  
Shengjun Feng ◽  
Jing Yuan ◽  
Chen Wang ◽  
Tao Lu ◽  
...  
Keyword(s):  
Fruit Quality ◽  
Nutritional Quality ◽  
Rapid Development ◽  
Genetic Regulation ◽  
Control Network ◽  
Flavor Quality ◽  
High Quality ◽  
Cucumis Sativus L ◽  
Cucumber Fruit

Cucumber is one of the most widely grown vegetables in China and an indispensable fresh fruit in the diet. With the development of society, the demand of people for cucumber quality is higher and higher. Therefore, cultivating high-quality cucumber varieties is one of the main goals of cucumber breeding. With the rapid development of biotechnology such as molecular marker, cucumber quality control network is becoming clear. In this review, we describe the formation mechanism of cucumber fruit quality from three aspects: (1) the commercial quality of cucumber fruit, (2) nutritional quality formation, and (3) flavor quality of cucumber fruit. In addition, the determinants of cucumber fruit quality were summarized from two aspects of genetic regulation and cultivation methods in order to provide ideas for cucumber researchers and cultivators to improve fruit quality.

scholarly journals Evaluation of Growth and Fruit Quality of Cucumber (Cucumis sativus L.) Irrigated with African Catfish Cultured Wastewater

2020 ◽  
Vol 27 (2) ◽  
pp. 95-100
Author(s):  
A.O. Akinwole ◽  
A.B. Dauda ◽  
E.B. Oyewole
Keyword(s):  
Cucumis Sativus ◽  
Growth Yield ◽  
Integrated System ◽  
Fish Growth ◽  
Well Water ◽  
African Catfish ◽  
Cucumis Sativus L ◽  
Cucumber Fruit ◽  
Negative Effect

This paper reports findings of an experiment carried out in a greenhouse to evaluate the growth and fruits quality of cucumber grown in plots irrigated with African catfish cultured wastewater compared with those irrigated with ordinary well water. Six circular African catfish culture tanks and six cucumber planter boxes were used for this experiment, three each for the fish cum cucumber integrated system and the control system of cucumber irrigated with ordinary well water. The results of the research indicated that fish cultured wastewater had a better influence on the plant height, the number, length, and diameterof leaves, as well as the diameter and length of the fruits. Six experts, each independently assessed fruits from the two treatments for juiciness, sweetness, taste and crispiness. The fruits were assessed by experts who though were aware of the purpose of the evaluation, did not know the identity of fruits they were assessing. The fish effluent fertigated cucumber fruits had a higher mean score in juiciness, sweetness, taste and crispiness. Wastewater from the African catfish production system thus exhibits the potential for reuse in the production of cucumber plants as it had no negative effect on the organoleptic quality of cucumber fruit produced. Keywords: Cucumis sativus, Fish, Growth, Yield, Wastewater.

scholarly journals The Ethanol Dip and Vapor Delays Chilling Injury of Cucumber (Cucumis Sativus L. cv. Ceylan), During Storage.

2018 ◽  
Vol 8 ◽  
pp. 1339-1349
Author(s):  
Rezzan Kasim ◽  
Mehmet Ufuk Kasim ◽  
Kubra Yasar ◽  
Tugce Sahin
Keyword(s):  
Decay Rate ◽  
Cucumis Sativus ◽  
Chilling Injury ◽  
Ethanol Treatment ◽  
Polystyrene Foam ◽  
Cucumis Sativus L ◽  
Cucumber Fruit ◽  
Different Doses

In this study, the effects of ethanol which is applied in different forms as liquid and vapor after harvest on chilling injury, color and overall quality of cucumber (Cucumis sativus L. cv. Ceylan) during storage were examined. For the liquid ethanol treatments, cucumber fruits were immersed in water comprising different concentration of ethanol for 5 minutes. In order to apply ethanol in the form of steam, a pad was firstly impregnated with ethanol at different doses. This pad was placed in a polystyrene foam dishes, and after the cucumber fruit is placed on it, the dishes wrapped with stretch film. The doses for used the both ethanol treatment as: Controldip(CD), Controlvapor (CV), 200 µL/L, 400 µL/L , 800 µL/L and 1600 µL/L. It was concluded that the liquid ethanol treatment at the dose of 1600 µL/L was effectively delayed of chilling injury, softening and decay rate.

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